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Global analysis of the dehydration-responsive mitochondrial proteome in rice (Oryza sativa L.) Dipak Gayen1, Pragya Barua1, Nilesh Vikram Lande1, Amit Kumar Dey2, Tushar K Maiti2, Subhra Chakraborty1 and Niranjan Chakraborty1* 1 National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India. 2 Regional Centre for Biotechnology, NCR Biotech Science Cluster, 3rd Milestone GurgaonFaridabad Expressway, Faridabad, Haryana 121001, India. Rice is the most important crop, representing the staple food for more than half the world's population. The rice crop requires larger amount of water throughout its life cycle when compared to other crop species. Water-deficit or dehydration causes severe threat to rice production in the rainfed areas across the world. Dehydration response in plants is a complex phenomenon, and is poorly understood. Plants exposed to dehydration greatly rely on the protection of cellular integrity to prevent physiological injury by orchestrating mitochondrial energy metabolism. A detailed understanding of global analysis of protein expression in mitochondria may lead to cellular adaptation against water-deficit conditions. To dissect the molecular mechanism, fourweek-old rice seedlings were subjected to progressive dehydration by withdrawing water, and the stress severity was assessed by physicochemical reactions and organellar architecture. The comparative analysis of the mitochondrial proteome using iTRAQ led to the identification of differentially expressed proteins with diverse cellular functions including energy production, cellular metabolism, cell signaling and translation. The metabolite identification and quantification, based on gas chromatography (GC)-MS analysis, indicated the pathways involved and the network topology of dehydration response in rice. Additionally, several of the identified differentially expressed proteins, previously not known to be involved in dehydration response, were validated by transcript analysis using qPCR and their localization to the mitochondria was confirmed by transient expression in Nicotiana benthiana. Further molecular analysis will provide new insight into the underlying mechanism of dehydration response and involvement of mitochondrial proteins for better adaptation against water-deficit conditions.