Download DRAFT Patient`s weight (kg) Recommended Volume (mL) of Blood

Patient’s
weight (kg)
<5
5-7
7-15
16-20
20+
Recommended
Volume (mL) of
Blood
Aerobic
1
3
4
8
10
Recommended volume
(mL) of blood if
anaerobic culture added
Aerobic
Anaerobic
0.5
0.5
1.5
1.5
3
1
5
3
5
5
If total blood obtained for blood culture is < 4 ml, use a pediatric blood culture bottle
If total blood obtained for blood culture is > 4 ml use adult blood culture bottles
If an anaerobic culture is desired place recommended volume of blood (up to 5 mls) into the aerobic
bottle and the remaining into the anaerobic bottle.
In general, the more blood that can be obtained, the better the potential yield on that blood culture.
*There may be clinical situations where the volume of blood that is obtained for sampling will differ
from recommendations according to patient condition and diagnosis. If an LIP wants a different volume
than the one listed, he or she should discuss that with the practitioner drawing the blood.
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diagnosis of infectious diseases: 2013 recommendations by the infectious diseases society of
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e22-e121.
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Multidisciplinary team review of best practices for collection and handling of blood cultures to
determine effective interventions for increasing the yield of true-positive bacteremias, reducing
contamination, and eliminating false-positive central line associated bloodstream infections.
Infection Control and Hospital Epidemiology, 43, 1222-1237.
Gaur, A., & Huskins, W. (2015). Guidance for collection of blood cultures from children & adolescents
with a central line. ().Children's Hospital Association.
Mayo Medical Laboratories. (2015). Recommended pediatric weight-based blood culture volumes.
Retrieved from http://a1.mayomedicallaboratories.com/webjc/attachments/128/2d2d720recommended-pediatric-weight-based-blood-culture-volumes.pdf
Lancaster DP, Friedman DF, Chiotos K, Sullivan KV. Blood Volume Required for Detection of Low Levels
and Ultralow Levels of Organisms Responsible for Neonatal Bacteremia by Use of Bactec Peds
Plus/F, Plus Aerobic/F Medium, and the BD Bactec FX System: an In Vitro Study. J Clin Microbiol
2015;53:3609-3613.
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detect common neonatal pathogens. J Pediatr 1996;129:275-278.
Yaacobi, N., Bar-Meir, M., Shchors, I., & Bromiker, R. (2015). A prospective controlled trial of the optimal
volume for neonatal blood cultures. The Pediatric Infectious Disease Journal, 34(4), 351-354.