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Biosafety Level III Biosafety Level 3 Introduction Suitable for work with infectious agents which may cause serious or potentially lethal disease as a result of exposure by the inhalation route. 2.5 Biosafety Level 3 Introduction Exposure potential to pathogens spread by aerosol n Infection serious, possibly lethal n Examples: n M. tuberculosis n St. Louis encephalitis virus n Coxiella burnetii n 2.5 Biosafety Level 3 Laboratory Facilities (Secondary Barriers) 2.5 Biosafety Level 3 Laboratory Facilities (Secondary Barriers) n BSL-1 and 2 Facilities PLUS: n Separate building or isolated zone n Double door entry n Directional inward airflow n Single-pass air; 10-12 air changes/hour 2.5 Biosafety Level 3 Laboratory Facilities (Secondary Barriers) n BSL-1 and 2 Facilities PLUS (cont.): n n n Enclosures for aerosol generating equipment Room penetrations sealed Walls, floors and ceilings are water resistant for easy cleaning 2.5 Biosafety Level 3 Laboratory Facilities ( Secondary Barriers) n BSL-1 and 2 Facilities PLUS: n Vacuum lines protected with liquid disinfectant traps or HEPA filters 2.5 Facility Design (Tertiary Barriers) n n Lab structure Lab ventilation 2.5 Biosafety Level 3 Standard Microbiological Practices 2.5 Biosafety Level 3 Safety Equipment (Primary Barriers) n BSL-1 and 2 Safety Equipment PLUS: n BSC class II or III to manipulate infectious material 2.5 Biosafety Level 3 Safety Equipment (Primary Barriers) n BSL-1 and 2 Safety Equipment PLUS: n Respiratory protection may be indicated 2.5 Biosafety Level 3 Special Practices n BSL-2 Special Practices PLUS: n n n Work in certified BSC Use bioaerosolcontaining equipment Decontaminate spills promptly 2.5 Biosafety Level 3 Special Practices Supervision § Supervisor is a competent scientist experienced working with agents § Establishes criteria for entry § Restricts access § Develops policies/procedures § Trains lab personnel 2.5 Biosafety Level 3 Special Practices Lab Personnel § Strictly follow guidelines § Demonstrate proficiency § Receive appropriate training § Report incidents § Participate in medical surveillance 2.5
