Download 11. Lab – Hematopoeisis

Hematopoeisis
VIBS 443/602
113
Peripheral blood smear (May-Grunwald-Giemsa)
110
Peripheral blood smear (Leishman-Giemsa)
eosinophil, basophil, and neutrophils
19761 Bone marrow 19761
Bone marrow smear; low power view, showing negative images of fat
globules
Megakaryocyte: contain many joined nuclei. Each probably contain 8-32
times a normal diploid amount of DNA
1. Nuclei of megakaryocyte
2. Red blood cell
Macrophage: contains greenish, iron-containing granules due to poor
quality. Differentiating cells have been torn away during preparation.
1. Macrophage
2. Lymphocyte
3. Neutrophil
1. Pronormoblast
2. Basophilic normoblast
3. Polychromatophilic normoblast
4. Orthochromatic normoblast
5. Myelocyte
6. Late metamyelocytes
1. Pronormoblast
2. Basophilic normoblast
3. Polychromatophilic normoblast
4. Orthochromatic normoblast
5. Myelocyte
6. Late metamyelocytes
1. Late promyelocyte
2. Late myelocyte/early metamyelocyte
3. Nearly mature PMN
1. Smudge cell
2. Promyelocyte
3. Myelocyte
4. Metamyelocytes
1. Pronormoblast
2. Metamyelocytes
3. Band cell
Bone marrow: EM 12a
•
Reticular cell
•
Developing red blood cell
Bone marrow; EM 8a
1. Lymphocyte
2. PNM
3. Red blood cell
4. eosinophil
Bone marrow; EM 12b
1. PMN
2. Immature neutrophil
3. Red blood cell
Routine H&E of bone
•
Marrow spicules
•
Lipid in fat cells
H&E of bone marrow
1. megakaryocytes
19761
H&E of bone marrow:
•
Red blood cell
420
Rib
420
Rib
421
Tibia, fetal
583
83
112
Red bone marrow smear (May-Grunwald-Giemsa)
Cells of Erythropoisesis
Pronormoblast
round nucleus and thin rim of deep blue cytoplasm
Basophilic normoblast
nuclear condensation ("fractured nucleus") with a
deep blue cytoplasm
Polychromatophilic normoblast
cytoplasm has accumulated enough
hemoglobin so that it is no longer blue but a
bluish-gray to bluish-red
Orthochromatic normoblast
must have an almost mature cytoplasm with only
the faintest hint of blue: the nucleus is fully
Reticulocyte
no nucleus, mature cytoplasm with blue tint
Red blood cell
typical of mature cell seen in circulation
Cells of Granulopoiesis
Promyelocyte
oval nucleus with deep blue cytoplasm and azurophilic
granules
Myelocyte
cytoplasm becomes increasingly mature
(reddish/clear) but still contains blue, nucleus of
variable shape
Metamyelocyte
deeply indented nucleus with nearly mature
cytoplasm (no longer blue)
Band cell
nucleus just prior to lobulation with fully mature
cytoplasm
Neutrophil (mature)
lobulated nucleus with fully mature cytoplasm