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Hematopoeisis VIBS 443/602 113 Peripheral blood smear (May-Grunwald-Giemsa) 110 Peripheral blood smear (Leishman-Giemsa) eosinophil, basophil, and neutrophils 19761 Bone marrow 19761 Bone marrow smear; low power view, showing negative images of fat globules Megakaryocyte: contain many joined nuclei. Each probably contain 8-32 times a normal diploid amount of DNA 1. Nuclei of megakaryocyte 2. Red blood cell Macrophage: contains greenish, iron-containing granules due to poor quality. Differentiating cells have been torn away during preparation. 1. Macrophage 2. Lymphocyte 3. Neutrophil 1. Pronormoblast 2. Basophilic normoblast 3. Polychromatophilic normoblast 4. Orthochromatic normoblast 5. Myelocyte 6. Late metamyelocytes 1. Pronormoblast 2. Basophilic normoblast 3. Polychromatophilic normoblast 4. Orthochromatic normoblast 5. Myelocyte 6. Late metamyelocytes 1. Late promyelocyte 2. Late myelocyte/early metamyelocyte 3. Nearly mature PMN 1. Smudge cell 2. Promyelocyte 3. Myelocyte 4. Metamyelocytes 1. Pronormoblast 2. Metamyelocytes 3. Band cell Bone marrow: EM 12a • Reticular cell • Developing red blood cell Bone marrow; EM 8a 1. Lymphocyte 2. PNM 3. Red blood cell 4. eosinophil Bone marrow; EM 12b 1. PMN 2. Immature neutrophil 3. Red blood cell Routine H&E of bone • Marrow spicules • Lipid in fat cells H&E of bone marrow 1. megakaryocytes 19761 H&E of bone marrow: • Red blood cell 420 Rib 420 Rib 421 Tibia, fetal 583 83 112 Red bone marrow smear (May-Grunwald-Giemsa) Cells of Erythropoisesis Pronormoblast round nucleus and thin rim of deep blue cytoplasm Basophilic normoblast nuclear condensation ("fractured nucleus") with a deep blue cytoplasm Polychromatophilic normoblast cytoplasm has accumulated enough hemoglobin so that it is no longer blue but a bluish-gray to bluish-red Orthochromatic normoblast must have an almost mature cytoplasm with only the faintest hint of blue: the nucleus is fully Reticulocyte no nucleus, mature cytoplasm with blue tint Red blood cell typical of mature cell seen in circulation Cells of Granulopoiesis Promyelocyte oval nucleus with deep blue cytoplasm and azurophilic granules Myelocyte cytoplasm becomes increasingly mature (reddish/clear) but still contains blue, nucleus of variable shape Metamyelocyte deeply indented nucleus with nearly mature cytoplasm (no longer blue) Band cell nucleus just prior to lobulation with fully mature cytoplasm Neutrophil (mature) lobulated nucleus with fully mature cytoplasm