Download theoretical and practical course techniques in molecular biology and

 THEORETICAL AND PRACTICAL COURSE TECHNIQUES IN MOLECULAR BIOLOGY AND GENETIC ENGINEERING OF PLANTS AND BACTERIA Sheda Science & Technology Complex (FMST) Km 10 (from Gwagwalada) Abuja – Lokoja Expway, Abuja, NIGERIA PRELIMINARY WORKSHOP PROGRAMME Date Time Activity Venue Resource Sun July 3, 2016 All day 6.00 – 8.00 pm 7.00 a.m. Arrival of resource persons and participants /Registration Welcome reception Breakfast Registration OPENING CEREMONY Introduction of resource persons and participants Workshop overview Presentations by participants (interaction with resource persons) Pre-­‐‑course Questionnaire Welcome Address Tea Break / Photographs LECTURE Laboratory Safety / Biosafety: Principles and Practices The Gene and Gene Expression (Review) Introduction of participants to the Laboratory and grouping them in 5 working Units and distribution of Lab coats. Lunch Break Laboratory safety and biotechnology regulations & technique Sterile technique and Stock preparation of needed reagents (Each group) Types of culture Media and their Preparation and storage (Bacteria and rice) for transformation Country Presentations/ expectations Departure to hotel(s) Hotel Secretariat Hotel Admin Bldg. Biotechnology Advanced Lab (BAL) Secretariat SHESTCO Admin Bldg Lecture room DG, SHESTCO All Dr. Solebo-­‐‑
Ajenifuja (NABDA) Prof. Ralf Greiner Mon July 4, 2016 9.00 a.m. 10.00 a.m. 10.20 a.m. 10.30 a.m. 11.30 p.m. 12..30 p.m. 1.00 pm 2.00 p.m. 5.00 p.m. 6.00 p.m. Lecture room Laboratory Dr. Afolabi / BAL Staff Laboratory Lecture room Odeyemi / Solebo-­‐‑
Ajenifuja BAL Staff BAL Staff 1 Tuesday July 5, 2016 9.00 a.m. 10.00 am 11.00 a.m. 11.30 am 12. 30 a.m. 1.30 p.m. 2.30 p.m. 5.00 p.m. Wednesday 9.00 a.m. July 6, 2016 10.30 a.m. 11.00 a.m. 1.30 p.m. 2.30 p.m. Thursday July 7, 2016 5.30 p.m. 9.00 a.m. 11.30 a.m. Overview of methods in conventional and plant biotech in crop improvement Overview of Recombinant DNA Technology Tea / Coffee Break Vectors: properties, types and their various usages. Promoters and their uses Techniques in Molecular Biology and Basic cloning techniques Theory -­‐‑ Restriction endonucleases, De-­‐‑
phosphorylation, Ligases and new recombinants Electrophoresis etc. Lunch Break Culturing of rice seeds on Callus Induction medium and growing of bacteria in broth Departure to hotel(s) Lecture room Dr. Onasanya Dr. Onasanya Lecture room Dr. Afolabi Lecture room Dr. Afolabi Laboratory BAL Staff Practical: Observation of previous lab work and mini discussion on the (media and bacterial culture) Basic Cloning techniques: Plasmid extraction, restriction digestion, de-­‐‑phosphorylation, and ligation Tea Break Continuation of practical Bacterial(E. coli (dH5α) transformation with vectors (plasmids) – Chitinase and heat shock genes, agarose gel, purification of DNA from gel etc. Lunch Break Continuation of Practical: Departure to hotel(s) Plant and Bacterial Transformation Techniques: Definitions, Plant Transformation techniques, Selectable marker genes, Problems associated with gene transfer Transgenes, transgene expression and transgene behavior Tea / Coffee Break Laboratory All Afolabi, Solebo, Odeyemi and BAL Staff Afolabi Solebo Odeyemi Dr. Onasanya / Dr. Afolabi Prof. Ralf Greiner Lecture room 2 Thursday July 7, 2016 (cont’d) 12.00 p.m. Laboratory Afolabi / Solebo/ Onasanya and BAL Staff Practical: Sub culturing of rice callus into fresh medium and media preparation for rice transformation Departure to hotel(s) Genetic markers in plant transformation: selectable and screenable / reporter marker) PCR (and qPCR principle and practice Tea / Coffee Break Methods of Selection marker gene(s) removal and why Lunch Break Practical Preparation of all media and reagents for Agrobacteria-­‐‑ mediated transformation of rice with chitinase / heat shock genes Departure to Hotel(s) Usuma Dam, Jabi lake and Abuja City Tour FREE AND PERSONAL TIME ACTIVITIES Laboratory Odeyemi and BAL Staff Lecture Afolabi Lecture Prof. Ralf Greiner Lecture Afolabi Laboratory BAL Staff OUTING DAY Agrobacterium transformation of rice calli (co-­‐‑cultivation) Tea / Coffee Break Agrobacterium transformation of rice calli cont’d Laboratory Laboratory 1.00 p.m. Lunch Break 2.00 p.m. Practical Restriction enzymes digestion of λ DNA, running of the product on agarose and viewing Review of Workshop to date and discussions Departure to Hotel(s) Practical: Agrobacterium-­‐‑mediated transformation of rice (cont’d) Transfer of co-­‐‑cultivated explants on CIM Tea Break Laboratory Classrom Laboratory All resource persons Afolabi Solebo Odeyemi Transformation of A. tumefaciens 1.30 p.m. 2.30 p.m. 5.30 p.m. Friday 9.00 a.m. July 8, 2016 10.15 a.m. 11.30 a.m. 12.00 p.m. 1.30 p.m. 2.30 p.m. 5.30 p.m. Saturday July 9, 2016 Sunday July 10, 2016 Monday 9.00 a.m. July 11, 2016 10.30 p.m. 11.00 p.m. 4.00 p.m. Tuesday July 12, 2016 Tuesday Practical: 5.00 p.m. 9.00 a.m. 11.00 a.m. with rice transformation vectors (Cold shock and Electroporation) Lunch Break 3 July 12, 2016 11.30 p.m. 1.30 p.m. 2.30 p.m. 4.00 p.m. 5.00 p.m. Wednesday 9.00 a.m. July 13, 2016 11.30 a.m. 12.00 p.m. 1.30 p.m. 2.30 p.m. Thursday July 14, 2016 5.00 p.m. 9.00 a.m. 10.00 a.m. 10.30 a.m. 12.30 p.m. 1.30 p.m. 2.30 p.m. 3.30 p.m. Friday July 15, 2016 4.30 p.m. 5.30 p.m. 9.00 a.m. 10.00 a.m. 11.00 a.m. 12.00 p.m. Saturday July 16, 2016 Agrobacterium-­‐‑mediated transformation (cont’d) Laboratory Lunch Break Research Grant Proposal writing IPR Issues Departure to Hotel(s) Practical: GUS Assay Lecture room Lecture room Laboratory Tea Break Practical: DNA extraction, PCR, electrophoresis of transgenic Lunch DNA extraction, PCR, electrophoresis of transgenic and discussion on results Departure to Hotel(s) Plant regeneration, organogesis and Somatic embryogenesis Laboratory Tea / Coffee Break Practical Transfer of co-­‐‑cultivated rice calli to Pre-­‐‑regeneration Medium. Overview of global biosafety practices – environmental, food and feed, socio-­‐‑economic aspects Lunch Overview of successfully transferred crops/ traits Process for commercial release of GM crops Group Discussions/Interactions Departure to hotel(s) Designing product oriented research Post course evaluation /assessment Summary of course accomplishments DG/CEO Lunch/ Award of certificates END OF WORKSHOP Departure Laboratory Afolabi Lecture room Prof. Ralf Greiner Lecture room Afolabi Dr. Addae (AATF) Laboratory Lecture room Lecture room Lecture room Afolabi Solebo Odeyemi Prof. Onyenekwe Solebo-­‐‑Ajenifuja All Resource persons All Resource persons All Resource persons Lecture room All All Resource persons All All All 4