Download Anti-Hsp70 antibody [EP1007Y] (HRP)

Product datasheet
Anti-Hsp70 antibody [EP1007Y] (HRP) ab198322
2 Images
Overview
Product name
Anti-Hsp70 antibody [EP1007Y] (HRP)
Description
Rabbit monoclonal [EP1007Y] to Hsp70 (HRP)
Conjugation
HRP
Tested applications
Suitable for: WB, IHC-P
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
corresponding to Human Hsp70 aa 600 to the C-terminus (C terminal).
Positive control
WB: HeLa, K562 and NIH3T3 whole cell lysates. IHC-P: Normal human tonsil tissue sections.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-Hsp70 antibody [EP1007Y] (ab45133)
Anti-Hsp70 antibody (Alexa Fluor® 488) [EP1007Y] (ab197870)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C.
Stable for 12 months at -20°C. Store In the Dark.
Storage buffer
pH: 7.4
Preservative: 0.1% Proclin
Constituents: PBS, 30% Glycerol, 1% BSA
Purity
Immunogen affinity purified
Clonality
Monoclonal
Clone number
EP1007Y
Isotype
IgG
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Applications
Our Abpromise guarantee covers the use of ab198322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
WB
1/5000. Detects a band of approximately 85 kDa.
IHC-P
1/167. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC
staining protocol.
Target
Function
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation
and mediate the folding of newly translated polypeptides in the cytosol as well as within
organelles. These chaperones participate in all these processes through their ability to
recognize nonnative conformations of other proteins. They bind extended peptide segments with
a net hydrophobic character exposed by polypeptides during translation and membrane
translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a
post-attachment receptor for the virus to facilitate entry into the cell.
Tissue specificity
HSPA1B is testis-specific.
Sequence similarities
Belongs to the heat shock protein 70 family.
Cellular localization
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Anti-Hsp70 antibody [EP1007Y] (HRP) images
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All lanes : Anti-Hsp70 antibody [EP1007Y]
(HRP) (ab198322) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma
cell line) Whole Cell Lysate
Lane 2 : K562 (Human erythromyeloblastoid
leukemia cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic
fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Western blot - Anti-Hsp70 antibody [EP1007Y]
(HRP) (ab198322)
Developed using the ECL technique
Performed under reducing conditions.
Observed band size : 74 kDa
Exposure time : 10 seconds
This blot was produced using a 4-12% Bis-tris
gel under the MOPS buffer system. The gel
was run at 200V for 50 minutes before being
transferred onto a Nitrocellulose membrane at
30V for 70 minutes. The membrane was then
blocked for an hour using 3% milk before
being incubated with ab198322 overnight at
4°C. Antibody binding was visualised using
ECL development solution ab133406.
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IHC image of Hsp70 staining in a section of
formalin-fixed paraffin-embedded normal
human tonsil*, performed on a Leica BOND.
The section was pre-treated using heat
mediated antigen retrieval with sodium citrate
buffer (pH6, epitope retrieval solution 1) for
20mins. The section was then incubated with
ab198322 at 1/167 dilution, for 15 mins at
room temperature. DAB was used as the
chromogen. The section was then
counterstained with haematoxylin and
Immunohistochemistry (Formalin/PFA-fixed paraffin-
mounted with DPX. The inset negative control
embedded sections) - Anti-Hsp70 antibody
image is taken from an identical assay without
[EP1007Y] (HRP) (ab198322)
primary antibody.
For other IHC staining systems (automated
and non-automated) customers should
optimize variable parameters such as antigen
retrieval conditions, primary antibody
concentration and antibody incubation times.
*Tissue obtained from the Human Research
Tissue Bank, supported by the NIHR
Cambridge Biomedical Research Centre.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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