Download transcription factor IIB (TFIIB)

2942 Nucleic Acids Research, 1993, Vol. 21, No. 12
Complete nucleotide sequence of an archaeal (Pyrococcus
encoding a homolog of eukaryotic
transcription factor IIB (TFIIB)
woeset) gene
Roberta Creti, Paola Londei and Piero Cammarano*
Istituto Pasteur-Fondazione Cenci Bolognetti, Universita di Roma 1, Dipt. Biopatologia Umana, Sez.
Biologia Cellulare, Policlinico Umberto 1, Viale Regina Elena 324, 00161, Rome, Italy
EMBL accession no. X70668
Received May 7, 1993; Accepted May 13, 1993
We have previously reported the sequence of the streptomycin
(str) operon-equivalent of the archaeum Pyrococcus woesei (1).
Subsequently, Ouzounis and Sander found that a partial ORF
located downstream (and on the complementary strand) of the
P.woesei str-operon-equivalent cluster, encodes the C-terminal
152 amino acids of a polypeptide that shares significant similarity
with the eukaryotic transcription factor TFIB (2). We have now
sequenced the P. woesei DNA region encompassing the missing
(N-terminal) portion of the TFIIB gene and the upstream 296
nucleotides (EMBL Data Bank accession number X70668). The
archeal gene is preceeded (at -73) by a consensus promoter.
The structural similarities between the P. woesei and the
eukaryotic TFIIB are shown in Figure 1. The archaeal sequence
(300 amino acids) is 27.5% -33.8% identical with that of eukarya
and exhibits unique structural motifs characteristic of eukaryotic
TFII (4-6) such as (i) an imperfect amino acid repeat
(encompassing residues 136-182 and 232-278) with 38.3%
identity and 63.8% similarity between the N-terminal and the
C-terminal repeats (ii) an intervening sequence characteristically
rich (32%) in basic amino acids between repeats (iii) Zn(ll)-finger
motif (Cys-X2-Cys-XI5-Cys-X2-Cys) located in close proximity
to the N-terminus and (iv) a predicted amphipatic ca-helix sitite
at the end of the N-terminal repeat (residues 167-184, P. woesei
numbering). On the whole, the distinguishing structural motifs
of TFlIB appear to predate the phylogenetic divergence of archaea
and eukarya and have been stringently conserved upon ensuing
evolution.
ACKNOWLEDGEMENT
R.Creti is recipient of a fellowship from the Fondazione A.
Buzzati-Traverso.
REFERENCES
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
Creti,R. et al. (1991) J. Mol. Evol. 33, 332-342.
Ouzounis,C. and Sander,C. (1992) Cell 71, 189-190.
Sharp,P.A. (1991) Nature 351, 16-18.
Brown J.W. et al. (1989) CRC Crit. Rev. Microbiol. 16, 287-338.
Malik S. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 9553-9557.
Pinto,I. et al. (1992) Cell 68, 977-988.
Ha,I. et al. (1991) Nature 352, 689-695.
Wampler,S.L. and Kadonaga,J.T. (1992) Genes and Develop. 6,1542-1552.
Hisatake,K. et al. (1991) Nucleic Acids Res. 19, 6693.
Tsuboi,A. et al. (1992) Nucleic Acids Res. 20, 3250.
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Figure 1. Alignment of the P.woesei TFIB sequence with those from Saccharomyces cerevisiae (Sce) (5), Drosophila melanogaster (Dme) (7), Xenopus laevis
(Xa) (8), Homo sapiens (Hsa) (6) and rat (9). Asterisks identify the Zn finger motifs. Arrows indicate the N-terminal and the C-terminal elements of the direct
repeat; plus symbols identify the putative amphipatic a-helix of the P.woesei TEIB sequence. Pairwise identities (percent) between all sequences are shown in a
matrix form.
*
To whom correspondence should be addressed