Download Intestinal Parasites Should Be Considered in HIV/AIDS

Intestinal Parasites Should Be Considered in HIV/AIDS Cases
Yaşar Ali Öner (1, *), Taner Yıldırmak(2), Sezer Sepetçioğlu (1), Semih Altıparmak(1), Kevser Atalık(1), Hayriye Kırkoyun Uysal(1), Özer Akgül(3)
1. Istanbul University, Istanbul Faculty of Medicine, Department of Medical Microbiology, Division of Parasitology, Istanbul, Türkiye.
2. Okmeydanı Training and Research Hospital, Department of Clinical Microbiology and Infectious Diseases, Istanbul, Türkiye.
3. Üsküdar University, Vocational School of Health Services, Istanbul, Türkiye.
*. Presenting Author
Objectives:
cayetanensis,
The intestinal spore-forming protozoa
Microsporidia species (5).
Cryptosporidium
and
have
important
methods,
be
positive
species
with
respectively.
for
these
parasitic infections should be performed
in HIV/AIDS cases.
Furthermore,
Microsporidia species positive samples
Keywords:
Among 91 feces included in the study
were examined with species-specific
HIV/AIDS,
clinical manifestations, diagnosis, and
were
the
PCR primer to detection of the definitive
Intestinal Parasites,
treatment (1, 2). Intestinal parasitic
prevalence
spore-
species of Microsporida, and 5 were
Staining Techniques,
infection has been a major source of
forming
sp.,
found as Enterocytozoon bieneusi, 8
Serological Techniques,
morbidity in many countries especially
Cryptosporidium sp. and Microsporidia
were
Molecular Techniques
among HIV/AIDS cases (3, 4). The aim
sp.) by different staining, serological and
intestinalis, and 2 were found as mixed
of
determine
molecular techniques. Modified Ziehl-
infection with Enterocytozoon bieneusi
prevalence of rare intestinal parasites
Neelsen staining and PCR techniques
and
and its association with immunological
were used to detection of Cyclospora
combination.
status and risk factors among HIV/AIDS
cayetanensis; and 1 and 2 samples
cases in Istanbul, Turkey.
were found to be positive for this
Conclusion:
parasite
methods,
Presented above are the results of the
this
disease
study
in
to
Results:
epidemiology,
similarities
found
Microsporidia
are identified gastrointestinal pathogens
that
were
pathogenesis,
was
to
examined
of
to
the
protozoa
determine
intestinal
(Cyclospora
with
these
found
as
Encephalitozoon
Encephalitozoon
intestinalis
Methods:
respectively.
Ziehl-Neelsen
first epidemiological study in Turkey on
Fecal samples were collected from 91
staining, ELISA and PCR techniques
the intestinal spore-forming protozoa
individuals with confirmed diagnosis of
were
prevalence in people with primary or
HIV. Modified Ziehl-Neelsen staining,
Cryptosporidium sp.; and 0, 1 and 1
secondary
trichrome
samples were found to be positive for
conclusion, we recommend considering
ImmunoSorbent Assay (ELISA), indirect
Cryptosporidium
infection
fluorescent antibody (IFA) and real-time
methods,
polymerase chain reaction (PCR) with
staining, IFA and PCR techniques were
Even
specific primers were used for detection
used
antiretroviral therapy (ART) are clearly
and confirmation of Cyclospora
species; and 18, 14 and 15 samples
staining,
Enzyme-Linked
1.
5.
Modified
used
to
to
detection
sp.
respectively.
detection
of
with
of
these
Trichrome
Microsporidia
immunodeficiencies.
with
the
intestinal
In
spore-
forming protozoa in HIV infected people.
the
success
rates
of
the
showed, routine diagnosis for intestinal
Sandra Wagner Cardoso, Thiago Silva Torres, Marilia Santini-Oliveira, Luana Monteiro Spindola Marins, Valdiléa Gonçalves Veloso, Beatriz Grinsztejn. Aging with HIV: a practical review. The Brazilian Journal of Infectious Diseases 2013; 17(4): 464-479.
2. Cegielski JP, Ortega YR, McKee S, Madden JF, Gaido L, Schwartz DA, et al. Cryptosporidium, enterocytozoon and cyclospora infections in pediatric and adult patients with diarrhea in Tanzania. Clin Infect Dis 1999; 28: 314-321.
3. Douek DC1, Roederer M, Koup RA. Emerging concepts in the immunopathogenesis of AIDS. Annu Rev Med. 2009; 60: 471-484.
4. Stark D, Barratt JL, van Hal S, Marriott D, Harkness J, Ellis JT. Clinical significance of enteric protozoa in the immunosuppressed human population. Clin Microbiol Rev 2009; 22: 634-650.
Karnika Saigal, Sumeeta Khurana, Aman Sharma, Rakesh Sehgal, Nancy Malla. Comparison of staining techniques and multiplex nested PCR for diagnosis of intestinal microsporidiosis. Diagnostic Microbiology and Infectious Disease. 2013; 77: 248–249.
Copyright © 2015
The presented work was supported by the Research Fund of Istanbul University. Project No. UDP-51694.
Yaşar Ali Öner, Taner Yıldırmak, Sezer Sepetçioğlu, Semih Altıparmak, Kevser Atalık, Hayriye Kırkoyun Uysal, Özer Akgül
[email protected]