Download Dr. D. Y. Patil Biotechnology And Bioinformatics Institute, Pune

Dr. D.Y. Patil Vidyapeeth’s
Dr. D. Y. Patil Biotechnology And
Bioinformatics Institute, Pune
Mail : [email protected], Website: biotech.dpu.edu.in.
M.Tech (Int). Bioitechnology Sem-II
Subject: Introduction to Biomolecules
Course Code: BS-202
Faculty: Dr. Manjusha Dake
L T P Hr C
3 0 2 5 4
Unit
Topics
Unit-1
Introduction
to
Biomolecules
Unit-2
Water
Types of Biomolecules in living system,
Structure-Function relationship.
-1
-1
Lecture
Serial
No
1
2
Water forms hydrogen bonds with polar solutes,
Water interacts electroststically with charged solutes,
Non polar compounds force energetically unfavourable
changes in the structure of water, Amphipathic
compounds in aqueous solution,
Solute affects the colligative properties of aqueous
solutions,
Osmosis and osmotic pressure.
-1
-1
3
4
-1
5
-1
6
-1
-1
7
8
1
9
-1
10
-1
11
-1
12
-1
13
-1
14
-2
15-16
Biological significance of carbohydrate and Classification
Unit -3
Carbohydrates of carbohydrate,
Monosaccharides: Aldehydes and ketones with multiple
hydroxyl groups,
Common monosaccharides occur in ring form: Formation
of hemiacetal and hemiketal. Fischer’s projection
formula,
Cyclization of D-Glucose to form D-Glucopyranose ring
structure,
Cyclization of D-Fructose to form D-Fructofuranose ring
structure,
Formation of glycosidic bond,
Disaccharides contain two monosaccharide units:
Structures for Maltose and Lactose,
No Of
Lectures
Unit
Unit -4
Lipids
Topics
No Of
Lectures
Lecture
Serial
No
Structure of sucrose,
Homopolysaccharides are stored forms of fuel: Structure
of Starch and Glycogen ,
Glycosaminoglycans are heteropolysaccharides of the
extracellular matrix,
Glycosaminoglycans: Structure and functionHyaluronate, Chondroitin sulfate, Keratan sulfate and
Heparin,
Glycoconjugates: Proteoglycan, Glycoproteins and
Glycolipids: Biological functions,
Glycoproteins are covalently attached Oligosaccharides:
Tissue glycoforms,
Structure and function of membrane glycoprotein:
Glycophorin,
Glycolipids and Lipopolysaccharides are membrane
components.
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17
-2
18-19
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20
-2
21-22
-3
23-24
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25
-1
26
-1
27
Biological functions
Fatty acids are hydrocarbon derivatives: Nomencleature
and occurrence of saturated and unsaturated fatty acids,
Physical properties of fatty acids: Solubility and Melting
points of saturated and unsaturated fatty acids,
Triacylglycerols are fatty acid esters of Glycerol,
Triacylglycerols provide stored energy and insulation
Structural lipids in membrane: General introduction
regarding structural lipids in membrane,
Glycerophospholipids –Parental compound-Phosphatidic
acid, types of phospholipids-phosphatidylethanolamine,
phosphatidyl choline, phosphatidyl serine, phosphadityl
inositol-4,5 bisphosphate,
Sphingolipids are derivatives of sphingosine:
Sphingolipids-a class of membrane lipids, structural
parent of sphingolipids as ceramide, sphingosine,
Sphingomyelin: Structure and location in mammals
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28
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29
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30
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31
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32
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33
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34
Unit
Unit-5
Amino acids
Unit -6
Proteins
Topics
No Of
Lectures
Glycosphingolipids: Neutral lipids: Cerebrosides,
Globosides, Complex Sphingolipids: Gangliosides:
Structure and location - GM, GD, GT and GQ series,
Sterols: have four fused carbon rings.
-1
Lecture
Serial
No
35
-1
36
Amino acids: amino acid share common structural
features: Naming carbon atoms in amino acids, Trivial
names and three letter abbreviations (Symbols) of
amino acids, optical activity and stereoisomerism in
amino acids,
Amino acids can be classified by R groups: Non polar
aliphatic R groups, Aromatic R groups. Aromatic R
groups, Polar Uncharged R groups,
Amino acids can be classified by R groups: Positively
charged (Basic) R gropus, Negatively charge (Acidic)
R gropus,
Amino Acids Can Act as Acids and Bases,
Amino acids have characteristic titration curves:
Titration curve of glycine. Applications of titration
curve of glycine.
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37
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38
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39
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40
Proteins:Amino acids are linked by peptide bonds to
form polypeptide chains,
Protein:The peptide Unit is rigid and planar: Peptide
unit is a rigid planar arrays of four atoms (N,C,H and
O),
Polypeptide chains can fold into regular structures
such as α-helix: Model of right handed α-helix along
with dimentions, stabilization of α-helix,
β -pleated sheets are stabilized by hydrogen bonding
between β strands: Conformation of dipeptide unit in a
β-srand. The polypeptide chain is almost fully
stretched out,
Ramchandran Plot: Ramchandran plot displays
allowed conformations of the main chain.
1
41
-1
42
-1
43
Unit
Topics
No of
Lectures
Unit-7
Nucleic acids
Nucleic acids: Structure of nitrogenous bases,
Ribose and Depxyribose sugar, Nucleosides and
nucleotides in DNA and RNA,
Backone structure of DNA-Formation of
phosphodiester bonds,
Watson-Crick Double Helical Model of DNA,
Nucleic acids: DNA double helix can be reversibly
melted. Hyperchromism in DNA,
A, B and Z types of DNA.
-1
Lecture
Serial
No
44
-1
45
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46
Books recommended:
1) Principles of Biochemistry: Lehninger and Nelson Cox 5th edition
2) Biochemistry: Lubert Stryer 4th edition
Evaluation scheme
Sno
Examination
Time
Marks
1
Minor test 1
45 Minutes
15
2
Minor test 2
45 Minutes
15
3
Internal & Assignment
4
Final Assessment
5
Total
40
2.5 hr
60
100
Practicals in Biomolecules:
1. Preparation of buffer solution.& demonstration of buffering action.
2. Determination of λmax & verification of Beer-Lambert’s law; Determination of molar extinction coefficient.
3. Preparation & standardization of laboratory reagents.
4. Qualitative detection of carbohydrates (Molish test, Benedicts test, Fehling’s test, lead acetate test,
inversion test, Seliwanoff’s test, Osazone test).
5. Titration curve of Glycine.
6. Qualitative detection of Lipids (solubility test, Acroline test (for glycerol), and test for cholesterol
7. Quantitative detection of Proteins
8. Introduction to molecular Graphics software RasMol.
9. Understanding Protein Structure using RasMol.
10.Understanding DNA structure using RasMol.