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University of Chester Department of Biological Sciences For general enquiries please contact Dr Matt Hartley [email protected] MRes Applied Science (Wildlife Behaviour and Conservation) A Masters of Research (MRes) programme differs from a conventional MSc course in terms of the balance between research and teaching. This particular MRes programme has been designed to enable extended periods of data collection in the laboratory or in the field not possible on our other postgraduate courses. This gives you the opportunity to work on a project, potentially with one of our research partners, either in the UK or overseas. Your work will contribute to ongoing research programmes and will address currently relevant questions in Wildlife Behaviour and Conservation, with peer-reviewed publication as a major focus. Students will spend between one and six months in the field. Wildlife Behaviour and Conservation is taught by staff who work with a diverse range of study species, including mammals, birds, fish and amphibians, in ecosystems as diverse as temperate woodlands, rainforests and coral reefs. They have close links with a variety of zoos, conservation organisations and NGOs both in the UK and world-wide. During Semester 1 (September to December) two taught modules will be delivered by our academic staff in Chester. These modules are designed to equip you with the key skills required for Masters level research. All MRes students will study a Wildlife Research Methods module, which provides an overview of field techniques, biodiversity data analysis, population models and research project management. Your second module will then focus on either Conservation Genetics or Behaviour and Welfare in Wildlife Conservation, depending on which is the most relevant area for your chosen research project. Compulsory Module BI1743 Wildlife Research Methods BI7140 Dissertation Project Optional Modules BI7130* Conservation Genetics BI7132* Behaviour and Welfare in Wildlife Conservation *You will select the module most relevant to your research project. From January until the following September, you will focus entirely on your research project, working on an individual basis with close support from your academic supervisor(s). Entry requirements: A minimum of 2:1 honours degree, which normally has a significant content of Biological Science (e.g. Biology, Biological Sciences, Environmental Science, Animal Behaviour etc), or substantial relevant working experience together with evidence of recent study or writing at an appropriate level. Overseas candidates should also be competent in English and have achieved, as a minimum, IELTS-6.5 Fees for this course are £4126 plus field and/or laboratory research fees to be discussed with the supervisor which will vary between project but average £3500. Candidates for this programme will select their preferred project upon application; however, in exceptional circumstances, we may consider students who wish to propose their own project. Applicants MUST apply for a specific project with a specific supervisor. To apply please fill in the application form found here: http://www.chester.ac.uk/research/degrees/mres Projects Being Offered in 2016/7 Distribution and Ecology of Yellow Necked Mice in North West England Dr Matt Hartley [email protected] The Yellow Necked Mouse (Apodemus flavicollis) is expanding its distribution into the North West of England. This study will examine distribution, population density and habitat preferences in this under studied animal. Methods will include live-trapping, use of population models and population mapping. Conservation of Endangered Primates in Ghana’s Rainforests (Multiple Students Accepted) [email protected] Dr Matt Hartley and Dr Christina Stanley Recent rainforest surveys have identified populations of endangered/critically endangered primates including roloway monkeys (Cercopithecus diana roloway), white-naped mangabeys (Cercocebus atys lunulatus), Geoffrey’s black & white colobus (Colobus vellerosus) and olive colobus (Procolobus verus), as well as the more common Lowe’s monkeys (Cercopithecus campbelli lowei) and lesser spot-nosed monkeys (Cercopithecus petaurista). However, there are still vast areas of rainforest which desperately require surveys, both to map the current distribution of these species and to identify their habitat requirements, allowing the identification of priority conservation areas and potential release sites. Community conservation projects are currently in operation which monitor and report illegal hunting and logging in some areas; further data on the efficacy of these schemes is also urgently required to ensure their success. This project will require two students to spend around four months in Ghana where they will carry out primate surveys in three rainforest regions using direct observations, primate presence evidence and camera trapping. This will form part of a long-term project working with West African Primate Conservation Action, a local NGO working in Ghana and Cote d’Ivoire. Conservation of Endangered Birds Species in Cambodia’s Dry Forests Dr Matt Hartley [email protected] The Bird Nest Protection Program was initiated by government partners, with support from WCS, to locate, monitor and protect nesting sites of globally threatened birds. Under the program, local people and contracted community rangers are rewarded with direct conservation payments to locate nests with a bonus if the nest successfully fledges. From 2002 to 2014 this incentive-based approach has safeguarded a total of over 3,631 nests consisting of ten globally threatened large waterbird species resulting in 6,481 fledglings. Data collected by the Bird Nest Protection Program is the primary method of verifying biodiversity indicators (nests identified each year and fledgling success) employed by WCS to monitor population trends of globally threatened bird species and assess the conservation impact of local livelihood schemes such as Ibis Rice. An MRes student would assist by working with existing data sets to analyze historical trends in nesting locations of globally threatened water bird populations by employing a nesting tree numbering system. They could also spend time in the field collecting further data to develop the project. (This project could include an optional period of 1-3 months in the field). Social and Predatory Behaviour in the Chestnut Mandibled Toucan (Ramphastos swainsonii) [email protected] Dr Matt Hartley There are few studies on the behaviour of toucan species. Anecdotal reports of social behaviour and co-operative breeding exist but the drivers of this affiliative behaviour are poorly understood. Toucans are generally considered frugivorous but have been seen feeding on eggs, nestlings and small mammals. Co-operative hunting behaviour of small birds has also been observed. This study will use citizen science survey techniques alongside field observations to study the feeding, predation, social and breeding of this key rainforest species. (Field work period 1-3 months to be discussed) Conservation of Tapirs in Costa Rican Wildlife Corridors Dr Matt Hartley [email protected] The tapir population of the adjacent Corcovado National Park is one of the best studied populations of the species providing comprehensive baseline population data to investigate abundance, habitat usage, resource needs and ecology of the tapirs in a contrasting modified environment. As the largest mammal in Costa Rica the tapir is an indicator species for the health and effectiveness of the biological corridor. The study will utilise three key methods. Firstly a comprehensive questionnaire study involving local communities will be conducted to record sightings, movements and fates of tapirs in the corridor. This will be supplemented by a web-based tool for tourist guides, officials and the public to record sightings This will identify key areas in which to focus survey efforts using camera traps. This data will be analysed using geographical and habitat modelling to identify key habitats, resources and movements in the corridor. This will enable management decisions to be made on further corridor regeneration and protection. (Field work period 3-6 months to be discussed) Feeding Ecology of the Two-Toed Sloth (Choloepus hoffmanni) in a human-modified environment. Dr Matt Hartley [email protected] Playa Matapalo is a settlement situated on the Pacific coast of Costa Rica consisting of tourism focused businesses. The environment has been significantly modified with the planting of non-native trees. In this environment there is a large population of two-toed sloth. Anecdotal reports claim that the high population density is due to the availability of the nuts from Terminalia catappa, known as the Almond Palm. This study will assess tree usage in the sloth, calculate population density and social behaviour in this modified environment and compare this to the neighboring wildlife reserve which has not be modified by non-native planting. (Field work period 1-3 months to be discussed Comparison of the Behaviour of White-faced Capuchin Monkeys (Cebus capuchinus) in Human Modified versus Natural Habitats Dr Matt Hartley [email protected] Playa Matapalo is a settlement situated on the Pacific coast of Costa Rica consisting of tourism focused businesses. The environment has been significantly modified with the planting of non-native trees. A population of White-faced Capuchin monkeys exploits this environment using the human habitation to its potential advantage. This has altered the foraging behaviour and increased human-primate interactions. This study will determine changes to behaviour, population structure and density caused by the use of a human modified environment by using populations in secondary and primary rainforest habitats where no or little human interaction occurs. (Field work period 1-6 months to be discussed) The use of ponies for conservation grazing in the UK Dr Christina Stanley [email protected] Grazing is essential for the maintenance of biodiversity in habitats such as grasslands, meadows, moorland and heathland which have historically been shaped by human activity. Ponies are often used for conservation grazing schemes as they are highly selective grazers, generally avoiding flowering plants and therefore allowing these to flourish. Pony faeces may also provide an important source of invertebrates for consumption by birds and other wildlife. However there is a general lack of evidence regarding the effects of pony grazing on both the biodiversity of vegetation and on invertebrate abundance. This project will involve both invertebrate/vegetation sampling and behavioural observations to quantify the effects of pony grazing on biodiversity and provide data to inform conservation grazing schemes. Population genetics of the Dee Estuary cockles: implications for resource management Dr Anna Muir [email protected] The Dee Estuary is a site of European nature conservation importance, particularly in terms of the bird life it supports. Natural Resources Wales manages the economically and environmentally important Dee Estuary cockle fishery. Careful management ensures that commercial exploitation of the cockle resource does not negatively impact cockle population size and ensures that there is sufficient food supplies for wildlife, in particular oystercatchers. In partnership with Natural Resources Wales, samples have been collected from the Dee Estuary cockle beds and will be collected from cockle beds in the vicinity of the estuary. The student will carry out DNA extractions and genetic/ genomic analyses to identify recruitment, gene flow and genetic diversity. Findings of this research will be relevant to the management of the Dee Estuary cockle fishery. Conservation Genetics of the Red Kite (Milvus milvus) Dr Ian McDowall and Amelia Glass (in the [email protected]) first instance please contact Globally the Red Kite is listed as a Threatened species by the IUCN, however the English population remains small, largely due to a sustained reduction in suitable habitat. To date little is known about the genetics of the contemporary English Red Kite population and the dispersal of reintroduced individuals, therefore genetic monitoring may be of significant value to future conservation efforts. This project offers the candidate an opportunity to access a unique sample collection of contemporary English Red Kites for the purposes of genetic monitoring and analysis (sourced through Natural England). Key areas of genetic research will include identification of molecular markers, analysis of population variation and biogeographical analysis, and contribution towards the sequencing of the whole mitochondrial genome for the species. Conserving the Largest Dry Forest Ecosystem: Cambodia’s Endangered Ungulates Dr Anna Muir and Dr Matt Hartley Almost all Indochinese large ungulates are now classified are globally threatened and the Eld’s Deer (Rucervus thiamine eldii), Indochinese Hog Deer (Axis porcinus) and Banteng (Bos javanicus) are listed as Endangered by the IUCN. Due to their rarity and cryptic nature, encounter rates with these species are low, making traditional field monitoring techniques ineffective. The University of Chester and the Wildlife Conservation Society (WCS) are developing a collaborative project to address the urgent need for data concerning species presence, abundance and habitat usage in Cambodia in order to inform conservation of these species. Banteng faecal samples will be collected during fieldwork in Cambodia and used for genetic analyses to identify population structure, genetic diversity and gene flow within this area. Further, using landscape genetic techniques, we will identify barriers to, and conduits for, dispersal in this species. This knowledge of how landscape relates to population structure will be used to inform conservation strategies. Monitoring the White-Faced Darter Dr Matt Geary [email protected] (Partially funded) (Leucorrhinia dubia) Reintroduction White-faced Darter are a habitat-specialist dragonfly which is currently classified as endangered on the British Red Data list. White-faced darter are patchily distributed in Britain with the largest populations in the Scottish Highlands and locally concentrated populations in Shropshire and Staffordshire. In recent years, re-introduction programmes have been undertaken to replace lost populations in Cumbria and, more recently, Cheshire. Larvae and eggs were initally reintroduced into Delamere forest Cheshire using their natural Sphagnum habitat as a transportation medium. Since them, further reintroductions have taken place along with monitoring and habitat management to improve the wider landscape for Whitefaced darter. Currently, monitoring efforts concentrate on exuviae counts which are known to be one of the most effective methods for monitoring Odonata populations. However, as the reintroduction progresses it becomes important to assess the accuracy of exuviae monitoring as well as to monitor the survival and movements of adult dragonflies. Information on dispersal and the use of flight corridors and habitat suitability assessments outside of the core reintroduction area would be especially useful for future habitat management. This MRes project would concentrate on these tasks and contribute to quantitative assessment of the reintroduction programme. The project is in partnership with Cheshire Wildlife Trust. This MRes project would be heavily fieldwork based and require good networking skills. Nest protection for woodland grouse Dr Matt Geary [email protected] (Partially funded) The nests of ground nesting birds are vulnerable to predation by terrestrial mammals. In the case of capercaillie (Tetrao urogallus) and black grouse (Tetrao tetrix), evidence indicates that foxes (Vulpes vulpes) and pine martens (Martes martes) are the two most significant mammalian predators. Pine martens are protected, but legal culling of foxes to reduce their impact on grouse is sometimes carried out. However, such management is expensive and raises ethical concerns. In addition, removal of foxes may result in compensatory increases in nest predation by martens. This MRes project would investigate the development of nonlethal management techniques for protecting the nests of capercaillie and black grouse. The project would involve field work in the highlands of Scotland testing the effectiveness of a number of nest protection techniques on protecting artificially constructed nests. The project is in partnership with Forest Enterprise Scotland, who would contribute to and support fieldwork equipment and costs. This project would suit a student who is keen to work in the field. It will require good networking skills and the ability to work independently. Using induced pluripotent stem cell technology to get doves into Noah’s Ark Professor Eustace Johnson [email protected] The Grenadian dove (Leptotila wellsi) is just one of many critically endangered species that are unlikely to avoid extinction, despite the efforts of conservation biologists. Their predicament is mirrored in some mammalian species, including the North African white rhino. In a major effort to avoid such extinction, stem cell biologists at the Scripps Institute in the USA have begun to apply newly developed methods, known as iPS (induced Pluripotent Stem) cell technology, to generate a bank of stem cells from the northern white rhino (Ceratotherium simum cottoni), which have the same potential for generating a whole organism as a normal embryonic stem cell. The iPS cells can be created from adult tissues, e.g. skin, and then either stored for later implantation into the womb of pseudo-pregnant rhinos of similar genetic make-up or they can differentiated to generate sperm and eggs for in vitro fertilisation. In principle, this will allow for the rebirth of the species if extinction were to occur. For birds, a similar approach could be adopted. However, very little research has been performed to test whether iPS technology will work with avian species. There is a further problem in that bird reproduction relies on the generation of an egg in the oviduct, and no research has examined whether iPS cells can be transplanted into birds to successfully generate eggs for hatching, let alone develop chicks. This research project will examine: (i) whether iPS technologies can be used to generate avian iPS cells; (ii) whether avian iPS cells can be differentiated to form sperm and eggs, which could in turn be used for in vitro fertilisation; (iii) whether a shell-less egg technique can be adapted to enable the develop of iPS cells or IVF generated zygotes to form chicks ex ova. The overriding aim is to examine the potential of iPS cells to preserve critically endangered species of avian origin.