Download Tag-it Violet™ Proliferation and Cell Tracking Dye

Version: 1
Revision Date: 12/21/2015
Tag-it Violet™ Proliferation and Cell Tracking Dye
Catalog# / Size
425101 / 1 kit (5 vials, 122.25 µg per vial, 500 µl DMSO)
Other Names
Tag-It Violet Proliferation Cell Tracking Dye
Ave. Rating
★★★★★ 2 reviews
Description
Tag-it Violet™ Proliferation and Cell Tracking Dye can be used for cell
tracking and for proliferation assays. Tag-it Violet™ passively diffuses into
the cell where esterases cleave acetoxymethyl esters (AM) on the molecule,
Tag-it Violet™ then covalently attaches to intracellular proteins enabling its
long-term retention.
Tag-it Violet™ is excited with a fluorescence wavelength of 395 nm, and
emits at 455 nm. Cells labeled wtih Tag-it Violet™ can be analyzed by flow
cytometry in instruments equipped with a violet laser, or visualized by
fluorescence microscopy using a DAPI filter.
Human peripheral blood mononuclear cells
were stained with Tag-it Violet™
Proliferation and Cell Tracking dye, and then
stimulated with PHA for four days. On day
four, cells were harvested, stained with CD3PE and the Tag-it Violet™ signal was
analyzed by flow cytometry.
Mouse spleen 72 hours after adoptive transfer
of Tag-it Violet™-labeled splenocytes
(purple). Nucleated cells are stained using 25
µM DRAQ™ (red). Image was captured at a
40X magnification.
See high resolution IF
data...
Preparation
The Tag-it Violet™ Proliferation and Cell Tracking Dye is composed of lyophilized Tag-it
Violet™ and anhydrous DMSO. For reconstitution, bring the kit to room temperature, add 50 µl
of DMSO to one vial of Tag-it Violet™ dye until fully dissolved.
Storage & Handling
The Tag-it Violet™ Proliferation and Cell Tracking Dye should be stored at -20°C upon receipt.
Do not open vials until needed. Once the DMSO is added to the Tag-it Violet™, use
immediately, or store at -20°C in a dry place and protected ifrom light, preferably in a dessicator
or in a container with dessicant for no more than one month. The unopened kit stored at -20°C
is guaranteed for 1 year after purchase.
Application
FC - Quality tested
IF - Validated
Recommended Usage
This lot has been tested by flow cytometry for in vitro cell proliferation. It can be used at
concentrations ranging from 1 - 20 µM for cell labeling. It is recommended that the reagent be
titrated for optimal performance for each cell type, culturing condition, or application.
Application Notes
The molecular weight of Tag-it Violet™ Proliferation and Cell Tracking Dye is 489 kD. The
maximum excitation and emission wavelengths of Tag-it Violet™ Proliferation and Cell
Tracking Dye are 395 nm and 455 nm, respectively. Each 122.25 µg of Tag-it Violet™
Proliferation and Cell Tracking Dye may be reconstituted with 50 µl of anhydrous DMSO to yield
a stock concentration of 5 mM.
Materials Provided:
5 vials x 122.25 µg Tag-it Violet™
500 µl anhydrous DMSO
Tag it-Violet™ Labeling Procedure:
1. Prepare stock solution by reconstituting 1 vial of lyophilized Tag-it Violet™ dye in 50 µl of
anhydrous DMSO to make a 5 mM solution.
2. Prepare a 5 µM working solution by diluting 1 µL of 5 mM Tag-it Violet™ stock solution in 1
mL PBS for every 1 mL of cell suspension (or at an optimal working concentration as
determined by titration).
3. Spin down and resuspend cells at 10-100 x 106 cells/mL in the Tag-it Violet™ working
solution.
4. Incubate cells for 20 minutes at room temperature or at 37°C, and keep protected from light.
5. Quench the staining by adding five times the original staining volume of cell culture medium
containing 10% FBS.
6. Pellet cells and resuspend in pre-warmed cell culture medium.
7. Incubate cells for 10 minutes.
8. After incubation, Tag-it Violet™ labeled cells are ready for downstream applications or
analysis.
Antigen Details
Structure
489 kD.
Gene ID
NA
Documentation
Technical data sheet
Certificate of Analysis
Safety Data Sheet
Reviews
Title
Rating
Date
Tag-it Violet™ Proliferation and Cell Tracking Dye
★★★★★
04/10/2017
In vivo Proliferation Measured with Tag-It Violet™ Labeled OT-II T Cells
★★★★★
04/14/2016
Related FAQs
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Related Categories
Cell Function/Viability Kits >
Buffers, Solutions & Chemicals > Chemicals >
Buffers, Solutions & Chemicals >
Antibodies and More > Cell Biology > Cell Cycle/DNA Replication > Tag-it Violet™ Proliferation and Cell Tracking Dye >
NeuroscienceProducts > Cell Death > Cell Viability Kits/Nuclear Staining Dyes >
Cell Function and Viability Kits >
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other violations that may occur with the use of our products.
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animal diagnostic, therapeutic or commercial use.
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