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REPRODUCTION REPRODUCTION: making offspring • A characteristic of living organisms is the ability to reproduce and pass on genetic material to maintain the survival of species. • Reproduction can be identified as either: 4. Asexual - involves a single parent - offspring or daughter cells are clones (ie. identical copies of each other and of the single parent) - offspring produced by mitosis 2. Sexual - Involves genetic contributions from two separate parents - Gametes ( sex cells) are the two sources in the form of eggs (female) and sperm (male) - Offspring are different from parents and each other - Variation due to meiosis TABLE: Differences between asexual and sexual reproduction. FEATURE ASEXUAL REPRODUCTION SEXUAL REPRODUCTION No. parents or parental contributions • one • two Processes involved • binary fission (prok) • cell replication involving mitosis (euk) • absent • gamete production involving meiosis (euk only) • no genetic variability; offspring are clones of single parent • faster • offspring vary from parents and each other • slower Fertilisation Offspring Time for completion • fusion of gametes required ASEXUAL REPRODUCTION • Is reproduction that does not involve the fusion (joining together) of two gametes (sex cells) is called asexual reproduction. In Prokaryotes: • binary fission (fission= ‘splitting’; binary= ‘two’) - replication of the circular molecule of DNA of the bacterial cell. - attachment of the two DNA molecules to the plasma membrane - lengthening of the cell - physical division of the cell into two via a constriction across the middle of the cell → each new cell contains one circular molecule of DNA • Simpler and faster than asexual reproduction in eukaryotic organisms • If resources are available one bacteria could produce 16 million descendants over an eight-hour period → process is called exponential growth • Organisms that are formed from the cells of a single parent are genetically identical to the parent. In Eukaryotes: • Some animals are capable of reproducing both asexually and sexually depending on the environmental conditions Forms of asexual reproduction in animals are: – Regeneration – parent breaks into 2 or more pieces and a new individual will arise from the piece e.g. planaria – Splitting in Half – e.g. amoeba – Budding – small groups of cells may bud or break away from the main organism and then settle on a substrate and reproduce mitotically to produce a new organism e.g. sponges, hydro – Parthenogenesis – ‘virgin birth’. Young are produced from unfertilised eggs. Offspring are identical to female parent e.g. honey bee • The ability to asexually reproduce is economically important. Examples of vegetative reproduction or vegetative propagation in plants: • Cuttings – piece of parent plant can be planted and new plants will grow from it e.g. roses • Runners (horizontal stems) – e.g. strawberries e.g. ferns and blackberry • Rhizomes – plants propagate through underground stems which sprout new daughter plants e.g. grasses and bracken • Suckers – plants that sprout from the roots of the parent plant • Plantlets without sex – e.g. ‘piggy-back’ plant. These are new small plants that drop off the parent and can strike • Gemme – small multicellular bodies produced in cup-like structures e.g. liverworts Artificial cloning of mammals Several techniques developed include: • Cloning using embryo cells - artificially separating the cells of an early embryo - transfer separated cells into surrogate mothers - results in identical twins, triplets or larger multiple identical siblings • Cloning using somatic nucleus - still an experimental and dramatic technique - first came to world attention through the birth of ‘Dolly’ in July 1996 - a somatic nucleus is cloned and transferred to an egg cell MEIOSIS • A cell divides twice to produce four cells with genetically different sets of chromosomes and half the original chromosome number. • This occurs in sexually reproducing organisms to produce GAMETES. • One diploid (2n) cell divides twice to form four genetically different haploid (n) cells. Meiosis Continued… Meiosis Continued… Meiosis Continued… Meiosis Continued… Meiosis Continued… Meiosis Continued… Meiosis Continued… Meiosis Continued… Meiosis Continued… • • Products of meiosis are not identical. The table below shows the possible outputs from meiosis – gametes in animals and spores in plants. SEXUAL REPRODUCTION IN PLANTS • Flower plays a key role in sexual reproduction Some Important Terms: • CALYX – made up of a ring of SEPALS, which are green and leaf-like • SEPALS – protect the flower when in bud • PETALS – form a ring inside the sepals (usually brightly coloured) an together form COROLLA • STAMEN – male reproductive parts form a ring inside corolla. Has a stalk called a FILAMENT with an ANTHER at its tip Sexual Reproduction in Plants Continued… • PISTIL – female reproductive part found at the centre of flower. At the base is an OVARY that extends into the STYLE which has an enlarged sticky part on top, the STIGMA • CARPEL – comprises an ovary, style and stigma. Some flowers have 1 carpel, some may have many Sexual Reproduction in Plants Continued… • Most flowering plants have both pollenproducing organs (stamens) and eggproducing organs (carpels). → these are called monoecious (= ‘one household’) plants, eg. Corn, oak • In other flowering plants the different flower types are on different plants → these are called dioecious (= ‘two household’) plants, eg. Holly, conifers Sexual Reproduction in Plants Continued… EGG PRODUCTION • An ovary contains at least one OVULE – a group of diploid (2n) cells attached to the wall of the ovary. • An inner cell divides by meiosis with three of the haploid cells degenerating • Mitosis eventually produces a 7-cell structure called the FEMALE GAMETOPHYTE or embryo sac • The cell closest to the micropyle (hole in layer around ovule) forms the egg Sexual Reproduction in Plants Continued… POLLEN PRODUCTION • Each anther contains four pollen sacs (diploid cells) • Each inner cell divides by meiosis and results in a 4 haploid spore • Each spore divides by mitosis once and develops into a POLLEN grain (microspore) containing 2 haploid cells, the tube cell and generative cell • The pollen grain is the MALE GAMETOPHYTE Sexual Reproduction in Plants Continued… POLLINATION • Occurs when pollen is transferred from an anther to a stigma • Transfer may be by: – Wind – Insects – Birds – Bats Two kinds of pollination exist: • 1. Self – Pollination – pollen from a flower falls onto the stigma of the same flower. Rarely happens because the anthers and ovules of the same flower mature at different times • 2. Cross – Pollination – more common. Occurs when pollen from one plant falls onto the stigma of another plant Sexual Reproduction in Plants Continued… FERTILIZATION • Once pollen is attached to stigma, the outer coat of the pollen grain begins to germinate • The POLLEN TUBE grows down through the style to the ovary • 2 nuclei enter the tube. One fertilises the egg and produces a zygote then an embryo. Second sperm fuses with the two polar nuclei and forms the ENDOSPERM, a food store for the developing embryo • Because 2 sperm cells involved – DOUBLE FERTILIZATION • Flowering plants DO NOT require presence of free water for fertilization Sexual Reproduction in Plants Continued… SEED FORMATION • After fertilization a SEED forms • Seeds consist of an outer seed coat formed from the outer layers of the ovule & an inner endosperm • Seeds are usually surrounded by a fruit that develops from the ovary of the plant Sexual Reproduction in Plants Continued… LIFE CYCLE OF A MOSS • Some plants require moisture for reproduction • Moss plants are of 2 distinct parts – the GAMETOPHYTE (haploid) and the SPOROPHYTE (diploid) • The green plant we see is the gametophyte. Male & female reproductive organs are on the end of separate stalks of the same plant or different ones • Eggs and sperm are produced by MITOSIS Sexual Reproduction in Plants Continued… • Sperm must swim to the egg therefore water must be present • Zygote formed after fertilization is DIPLOID and divide by MITOSIS to form the sporophyte • In the capsule on the tip of the sporophyte diploid cells within undergo MEIOSIS to give rise to SPORES (haploid) • Both flowering plants and moss show this ALTERATION OF GENERATION but it is more obvious in a moss plant Sexual Reproduction in Plants Continued… SEXUAL REPRODUCTION IN ANIMALS EXTERNAL FERTILIZATION • Eggs are laid and then male sheds sperm onto eggs and then fertilization occurs. • Many eggs released, increase chance of fertilisation but wastage is high. • Examples are animals that live in aquatic environments like most fish (not sharks), many amphibians, coral Sexual Reproduction in Animals Continued… INTERNAL FERTILIZATION • Copulation of male and female sees the transfer of sperm to the female. • The zygote is retained and develops in female. • Few eggs are produced, wastage is low. • Occurs in most land animals e.g. mammals, birds, reptiles, insects. Internal Fertilisation Continued… • Insects- complex genital structures at the end of the abdomen of a male insect enable him to couple with a female and transfer sperm packages into the reproductive tract of a female. • Reptiles- the first vertebrates (apart from sharks) to develop a penis. - the presence of a penis enables sperm to be directly transferred into the reproductive tract of a female. - high chance of fertilisation and low gamete wastage. - an evolutionary change was the egg with a protective outer shell, series of internal membranes and a food supply for the developing embryo. This self-contained aquatic environment allowed the egg to be laid on land Internal Fertilisation Continued… Lay Eggs No. of Eggs Parental care REPTILES BIRDS Moist environments Up to 150 (turtles) Some In nest 2-3 Lots • Yolk of egg provides nutrients for a developing zygote • Albumin (egg white) provides water Internal Fertilisation Continued… • Birds- sperm transferred through close contact of the urogenital openings (cloacas) of male and female birds. - also produce shelled eggs where the embryo develops • Mammals- in monotremes (platypus & echidna), embryonic occurs within a a shelled egg. - in marsupials, fertilised egg retained and embryonic development occurs in uterus. Young born at a very undeveloped stage. - in placental mammals, fertilised egg retained and embryonic development occurs in uterus. Young born at a much more developed stage. Sexual Reproduction in Animals Continued… • In some species, a single organism has both eggproducing and sperm-producing organs. These organisms are called hermaphrodites. eg. Garden snail and earthworm • When both sperm-producing and egg-producing organs are active at the same time it is called simultaneous (synchronous) hermaphrodism. • Animal species that can change sex are called sequential hermaphrodites. eg. Coral fish like anemonefish, wrasses • These fish start life as one sex and can transform to the other under certain conditions. Sexual Reproduction in Animals Continued… • Involves the fusion of two cells called gametes • Results in offspring that are quite different from each other • Essential process that ensures that when a ZYGOTE is formed after FERTILIZATION it has the same number of chromosomes as their parents – MEIOSIS • Meiosis occurs in reproductive tissues called GONADS • Gonads (‘gamete factories’) of female mammals – OVARIES; male mammals - TESTES Sexual Reproduction in Animals Continued… • Number of chromosomes in a somatic (body) cell is called the diploid number eg. Humans is 46 (2n = 46) • The number of chromosomes in a gamete (sex cell) is called the haploid number eg. Humans is 23 (n = 23) Sexual Reproduction in Animals Continued… REPRODUCTION IN HUMANS • Eggs have small amount of yolk • Sperm are motile • Internal fertilisation occurs so gametes are always in moist environment. MALE REPRODUCTIVE SYSTEM • Two testes are located in a loose pouch of skin – SCROTUM • Testes found outside body because lower temperature (35.5˚C) better for sperm development • Each testicle produces sperm and hormones • Onset of PUBERTY – Luteinising Hormone released from pituitary gland acts on testes and the testes produce TESTOSTERONE • Testosterone brings about: – – – – – Sex organ change Voice changes Muscle development Body hair growth Production of sperm Male Reproductive System Continued… Male Reproductive System Continued… MEIOSIS IN HUMAN MALES (SPERMATOGENESIS) • Sperm produced in TUBULES • Precursor sex cells develop during foetal development and remain dominant until puberty • Precursor sex cells reproduce by mitosis – results in primary spermatocytes • primary spermatocytes meiotically divide to produce 4 mature sperm Male Reproductive System Continued… Male Reproductive System Continued… Male Reproductive System Continued… Mature sperm move from: A testis ↓ EPIDIDYMIS – temporary storage ↓ VAS DEFERENS – runs from each epididymis to urethra ↓ URETHRA – sperm + a nutrient-rich fluid (SEMEN) secreted from SEMINAL VESICLE, COWPER’S GLAND and PROSTATE GLAND Male Reproductive System Continued… • During sexual arousal, large amount of blood enters PENIS, which becomes firm and erect • Semen leaves body through the urethra by EJACULATION • Each ejaculation – between 2.4 – 6mL of semen • Each mL of semen contains 50 – 100 x 106 sperm FEMALE REPRODUCTIVE SYSTEM • Female gametes – EGGS (or OVA) are produced in OVARIES • Precursor sex cells ↑ in no. by mitosis and enlarge to form potential egg cells • At birth each ovary has approx. 200,000 eggs • Ovaries also produce hormones – OESTROGEN & PROGESTERONE • MEIOSIS starts before birth but stops before completion and remains dormant until puberty Female Reproductive System Continued… Female Reproductive System Continued… MEIOSIS IN HUMAN FEMALES (OOGENESIS) • At puberty an ovarian cycle begins • One egg cell increases in size in response to hormonal signals • One egg cell divides – 2 cells of unequal size are produced - Smaller one is POLAR BODY and degenerates - Larger cell continues to develop within a FOLLICLE & is released at OVULATION into the FALLOPIAN TUBE • Meiosis continues after egg is released • The empty follicle becomes CORPUS LUTEUM Female Reproductive System Continued… Female Reproductive System Continued… Female Reproductive System Continued… • The egg moves into the funnel-like opening of a fallopian tube (10cm long) → UTERUS – muscular and shaped like an inverted pear • If egg is fertilised in fallopian tube then it becomes embedded in the uterus’ lining – ENDOMETRIUM • Endometrium is rich in blood vessels supplying developing EMBRYO with oxygen and nutrients • Lower end of uterus is CERVIX, which opens into VAGINA • Vagina has 2 functions: – Sperm enter female reproductive organs – Passageway for a baby during birth MENSTRUATION & MENSTRUAL CYCLE • From puberty → approx. 50 years old • Lasts 28 days • Governed by hormones – chemical messages transported in the bloodstream • Hormones released are: 1. Pituitary Gland - F.S.H – follicle stimulating hormone - L.H – luteinising hormone 2. Ovaries - Oestrogen - Progesterone Menstruation & Menstrual Cycle Continued… Menstrual cycle begins with: Pituitary gland releases FSH into the bloodstream stimulating eggs to develop. All but 1 degenerates. As ovum grows ovary releases OESTROGEN. Increasing levels causes 2 things: – Inhibits production of FSH so no more ova develop – Prepares lining of uterus (endometrium – capillaries) for fertilised ovum f. As ovum continues to ripen & oestrogen levels increase, the pituitary gland is stimulated to release LH. Menstruation & Menstrual Cycle Continued… LH causes release of ripe hormone ovum from ovary to nearby oviduct – OVULATION & causes follicle which held ovum to develop into CORPUS LUTEUM Corpus luteum releases PROGESTERONE into the bloodstream preventing pituitary releasing LH or FSH Increase in progesterone and decrease in LH causes basal body temp to rise by approx. 1˚C. This temp rise occurs just after OVULATION and is maintained till MENSTRUATION Menstruation & Menstrual Cycle Continued… If ripe ovum is not fertilised within a few days, corpus luteum stops producing progesterone and breaks up Decreasing the amount of progesterone causes the thickened lining of the uterus to break up and MENSTRUATION occurs 14 -16 days after ovulation Decrease in progesterone will stimulate release of FSH and a new ovum will start to grow and the cycle starts again Menstruation & Menstrual Cycle Continued… If Fertilization Occurs: c. If fertilization takes place, the placenta develops and produces 2 hormones responsible for preventing the corpus luteum breaking up so it continues to produce progesterone, therefore it: i. Prevents release of FSH so no more eggs mature ii. Prevents shredding of uterine lining NOTE: At time of ovulation egg is still undergoing meiosis. Two cells are produced and uneven. Again the smaller degenerates and becomes a polar body. The larger contains most of the cytoplasm and forms the mature egg. HUMAN LIFE CYCLE COMPARISON OF ANIMAL AND PLANT LIFE CYCLES