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Chapter 8 8.1 Energy and Life Energy • Plants and some other types of organisms are able to use light energy from the sun to produce food. • Organisms that make their own food are called autotrophs or producer. • Other organisms cannot use the sun’s energy directly. These organisms, called heterotrophs or consumer, obtain energy from the foods they consume. List some Autotrophs List some Heterotrophs ATP • One of the principal chemical compounds that cells use to store and release energy is adenosine triphosphate, or ATP. • ATP consists of adenine, a 5-carbon sugar called ribose, and three phosphate groups. ADP • Adenosine diphosphate (ADP) is a similar compound that has only two phosphate groups instead of three. • When a cell has energy available, it can store small amounts of energy by adding a third phosphate group to ADP, producing ATP. • The energy stored in ATP can be released by breaking the bond between the second and third phosphate groups. • Because a cell can subtract this third phosphate group, it can release energy as needed. • The characteristics of ATP make it exceptionally useful as the basic energy source of all cells. • Cells use energy from ATP to carry out many important activities, including active transport, synthesis of proteins and nucleic acids, and responses to chemical signals at the cell surface. 8.2 • Photosynthesis: An Overview PHOTOSYNTHESIS What is Photosynthesis? Plants using high-energy carbohydrates – sugars and starches – and oxygen, a waste product. What do you need for Photosynthesis? Van Helmont Priestley’s Experiment • 100 years after Van Helmont • Candle, Jar, plant Ingenhousz • showed that the effect observed by Priestley occurred only when the plant was exposed to light. • The experiments performed by van Helmont, Priestley, Ingenhousz, and other scientists reveal that in the presence of light, plants transform carbon dioxide and water into carbohydrates and release oxygen. PHOTOSYNTHESIS The sun Carbon dioxide from the air A plant Water from the soil PHOTOSYNTHESIS What does photosynthesis make? PHOTOSYNTHESIS Oxygen (waste) Food for plant (sugar) PHOTOSYNTHESIS water + Carbon dioxide sugar + Oxygen • Photosynthesis uses the energy of sunlight to convert water and carbon dioxide into high energy sugars and oxygen. • Plants use the sugars to produce complex carbohydrates such as starches. • Plants obtain the carbon dioxide they need for photosynthesis from the air or from the water in which they grow. The sun PHOTOSYNTHESIS Oxygen (waste) Carbon dioxide from the air Food for plant (sugar) Water from the soil PHOTOSYNTHESIS Where does photosynthesis take place? PHOTOSYNTHESIS vacuole Cell wall nucleus Cell membrane Chloroplasts!!! Plant Cell • In addition to water and carbon dioxide, photosynthesis requires light and chlorophyll. • Plants gather the sun’s energy with lightabsorbing molecules called pigments. • The plants’ principal pigment is chlorophyll. • There are two main types of chlorophyll: chlorophyll a and chlorophyll b. • Chlorophyll absorbs light in the blue-violet and red regions very well. • But it does not absorb light in the green region well. • Green light is reflected by leaves, which is why plants look green. • Any compound that absorbs light absorbs the energy in light. • When chlorophyll absorbs sunlight, much of the energy of the light is transferred directly to the electrons in the chlorophyll molecule, raising the energy level of the electrons 8.3 • The reactions of Photosynthesis PHOTOSYNTHESIS Chloroplasts are the GREEN parts of a plant cell. They contain a chemical called CHLOROPHYLL. Chlorophyll is a catalyst. A catalyst speeds up a chemical reaction, but doesn’t get used itself. PHOTOSYNTHESIS So PHOTOSYNTHESIS takes place in the Chloroplasts • Inside each chloroplast is a saclike structure known as a thylakoid. A stack of thylakoids forms a granum • Proteins in the thylakoid membrane organize chlorophyll and other pigments into clusters known as photosystems. • These photosystems are the light collecting units of chlorophyll. • The reactions of photosynthesis occur in two parts: – (1) the light-dependent reactions and – (2) the light-independent reactions, which are also called the Calvin cycle. • (Calvin cycle takes place in the stroma outside the thylakoid membranes) Light and Dark The Reactions of Photosynthesis • When sunlight excites electrons in chlorophyll, the electrons gain a great deal of energy. • electron carriers transfer high-energy electrons from chlorophyll to other molecules • A carrier molecule is a compound that can accept a pair of high-energy electrons and transfer them along with most of their energy to another molecule. • Formation of NADPH When NADP+ accepts a pair of highenergy electrons, it becomes NADPH. Light-Dependent Reactions • light-dependent reactions require light • The light-dependent reactions produce oxygen gas and convert ADP and NADP+ into the energy carriers ATP and NADPH. • The light-dependent reactions take place within thylakoid membranes of chloroplasts. • H+ ions cannot cross the membrane directly. However, the membrane contains a protein called ATP synthase (sin-thays) that allows H+ ions to pass through it. As H+ ions pass through this protein, the protein rotates like a turbine being spun by water in a hydroelectric power plant. As it rotates, ATP synthase binds ADP and a phosphate group together to produce ATP. The Calvin Cycle • The Calvin cycle uses ATP and NADPH from the light-dependent reactions to produce high-energy sugars. • the Calvin cycle does not require light, these reactions are also called the lightindependent reactions. Light-Dependent Reactions • Remove carbon dioxide • Begin with six carbon dioxide molecules • Produce high-energy sugars • Produce H+ ions within the thylakoid membrane Calvin cycle Reactions • Convert ADP and NADP+ into ATP and NADPH • Produce Oxygen gas • Occur in stroma of chloroplast • Occur in thylakoid membranes of chloroplast Factors Affecting Photosynthesis • Because water is one of the raw materials of photosynthesis, a shortage of water can slow or even stop photosynthesis. • Temperature is also a factor. Photosynthesis depends on enzymes that function best between 0°C and 35°C. • The intensity of light also affects the rate at which photosynthesis occurs. • After the light intensity reaches a certain level, however, the plant reaches its maximum rate of photosynthesis. The level at which light intensity no longer affects photosynthesis varies from plant type to plant type. The Steps in Photosynthesis • • • • • • • • • • • • • • • • • • 1) The light energy strikes the leaf, passes into the leaf and hits a chloroplast inside an individual cell. 2) The light energy, upon entering the chloroplasts, is captured by the chlorophyll inside a grana. 3) Inside the grana some of the energy is used to split water into hydrogen and oxygen. 4) The oxygen is released into the air. 5) The hydrogen is taken to the stroma along with the grana's remaining light energy. 6) Carbon dioxide enters the leaf and passes into the chloroplast. 7) In the stroma the remaining light energy is used to combine hydrogen and carbon dioxide to make carbohydrates. 8) The energyrich carbohydrates are carried to the plant's cells. 9) The energyrich carbohydrates are used by the cells to drive the plant's life processes. Review What is ATP? • adenosine triphosphate • ATP is used by all types of cells as their basic energy source. • For example, the energy needed by the cells of a soccer player comes from ATP. Can you explain this?