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B3 Question Answer Which process occurs in the mitochondria in cells? Why do the liver and muscle cells have large number of mitochondria? What is the function of the ribosomes? Respiration occurs in the mitochondria in cells. Liver and muscle cells require more energy so have more mitochondria. Ribosomes are the site of protein synthesis. Where are the ribosomes situated? What do you need to use to see ribosomes and some other structures in cells? What substance are chromosomes made of? What do chromosomes carry? What is the name of the coded information in genes? What does DNA control? What are proteins needed for? What is the structure of DNA? Ribosomes are in the cytoplasm A microscope. Chromosomes are made of DNA Coded information in the form of genes. The genetic code DNA controls the production of proteins Proteins are needed for growth and repair. DNA has two strands coiled to form a double helix Each strand contains chemicals called bases – there are four types Cross-links are formed between the strands by pairs of bases. Genes. Chromosomes are divided into areas called? What does each gene contain? Each gene contains a different sequence of bases Each gene codes for a particular protein. Proteins are made in the cytoplasm (ribosomes) A copy is needed because the gene itself cannot leave the nucleus. A, T, G and C A-T and G-C. The base sequence determines the amino acid sequence in proteins. What is the name of the molecule that takes information from the DNA to the ribosomes? How does DNA control cell function? mRNA takes information from DNA to the ribosomes What are the names of the scientists that first worked out the structure of DNA? DNA controls cell function by controlling the production of proteins – some of which are enzymes. Watson and Crick were the first to work out the structure of DNA. What information from other scientists did Watson and Crick use to determine the structure of DNA? Why wasn’t Watson and Crick’s discovery accepted immediately? X-ray data showing the double helix structure Data indicating that the bases occurred in pairs Work of scientists needs to be repeated or tested by other scientists before it is accepted. Give three examples of proteins. Collagen Where are proteins made? Why is a copy of the gene needed to make proteins? What are the four letters that represent the four bases in DNA? What are the complimentary base pairs?? What does the base sequence in DNA determine in proteins? What are proteins made of? What are the functions of proteins? Why do proteins have different functions? What do enzymes do to chemical reactions? What are enzymes made of? When do enzymes work best? Which biological processes do enzymes catalyse? What is meant by the ‘lock and key’ mechanism? Insulin Haemoglobin. Long chains of amino acids Structure (collagen) Hormones (insulin) Carrier molecules (haemoglobin) Enzymes Different proteins have different functions because they have different sequences of amino acids. Enzymes speed up chemical reactions Enzymes are proteins. Enzymes work best at certain temperatures Respiration Photosynthesis Protein synthesis. Enzymes have a high specificity for their substrate Enzymes have an active site that only fits certain substrates By changing the pH By changing the temperature. How can the rate of an enzyme catalysed reaction be changed? What happens to enzymes at high temperature or pH? What does denaturing do to the enzyme’s active site? What are gene mutations? What will different cells produce? What might gene mutations lead to? How can mutations occur? Are mutations always harmful? Do all cells use the full set of genes contained in each cell? How do genetic mutations lead to different protein production? Where does the energy for all life processes come from? What is the word equation for respiration? Which processes require energy from respiration? Respiration Glucose + oxygen carbon dioxide + water Muscle contraction Protein synthesis Control of body temperature in mammals C6H12O6 + O2 CO2 +H2O. ATP What is the symbol equation for photosynthesis? Apart from carbon dioxide and water what substance does respiration result in the production of? Enzymes denature at extreme temperature or pH Denaturing in an irreversible process and stops the enzyme from working properly Denaturing changes the shape of the active site. Changes to genes Different cells will produce different proteins. Gene mutations may lead to the production of different proteins. Spontaneously Due to radiation Due to chemicals Mutations are not always harmful; they can sometimes be beneficial or have no effect. No, some genes are switched off. Which cells are switched off determines the function of the cell Gene mutations change the sequence of bases in the gene which leads to changes in the amino acid sequence in the protein produced. What is ATP used for? Why can oxygen consumption be used as an estimate for metabolic rate? Why is the rate of respiration influenced by changes in pH and temperature? Why do breathing and pulse rate increase during exercise? Why does anaerobic respiration take place during hard exercise in addition to aerobic respiration? What is the name of the substance produced by anaerobic respiration that causes pain and fatigue? What is the word equation for anaerobic respiration? Which process produces the most energy per glucose molecule – aerobic or anaerobic respiration? What is fatigue in terms of lactic acid build up (oxygen debt) and how is this removed during recovery? ATP is the energy source for many processes in cells Because aerobic respiration requires oxygen Enzymes are used in respiration and changes in pH and temperature will cause the enzymes to denature. The muscle cells require more oxygen when exercising. Oxygen cannot be supplied to the muscles fast enough for all energy required to be produced by aerobic respiration. Lactic acid Glucose lactic acid Aerobic respiration Hard exercise causes lack of oxygen in cells This results in the incomplete breakdown of glucose. Continued panting after exercise to replace oxygen allowing aerobic respiration Heart rate increased to ensure that blood carries lactic acid away to the liver. In terms of cells, what is the difference between an amoeba and a dog? What are the advantages of being multicellular? Why do multicellular organisms require specialised organ systems? How are chromosomes arranged in most body cells? Why do chromosomes have to be copied to produce new cells? Why is cell division necessary? What is the name of the process that creates new cells for growth? What are cells that have two copies of each chromosome called? Why does DNA have to be replicated before cells divide? How does DNA replicate? What happens to chromosomes during mitosis? What are sperm and egg cells called? What happens to gametes during fertilisation? An amoeba is a unicellular organism and a dog is a multicellular organism. Allows organisms to be larger Allows for cell differentiation Allows organism to become more complex. Communication between cells Supplying the cells with nutrients Controlling exchanges with the environment. They are arranged in pairs. So that the new cells have exactly the same genetic information as the original. To replace worn out cells To repair damaged tissue For asexual reproduction Mitosis Diploid So when the cell divides the daughter cells have a complete set of DNA. The DNA ‘unzips’ to form single strands. New double strands forming by complementary base pairing. The chromosomes line up along the centre of the cell. They then divide The copies move to opposite poles of the cell. Gametes The gametes join during fertilisation. How many chromosomes do gametes have in comparison to body cells? When creating a unique individual where do half the genes come from? Why are sperm cells are produced in large numbers? Gametes have half the number of chromosomes compared to body cells. In sexual reproduction to produce a unique individual half the genes come from each parent. To increase the chance of fertilisation. How are gametes produced? What is the term for a cell with one copy of each chromosome? Why does fertilisation result in genetic variation? Gametes are produced by meiosis. Haploid Gametes combine to form a diploid zygote Genes on the chromosomes combine to control the characteristics of the zygote. To provide energy Why does a sperm cell have many mitochondria? What does the acrosome in a sperm cell do? Why is the chromosome number halved in meiosis? The acrosome releases enzymes to digest the egg membrane. One chromosome from each pair separate to opposite poles of cells in the first division In the second division the chromosomes divide and move to the opposite ends of the cell This makes each cell genetically different. What is the function of the red blood cells? What is the function of the white blood cells? What is the function of platelets? How is a red blood cell adapted to its function? Red blood cells carry oxygen around the body White blood cells protect the body from infection What is the function of plasma? What is the name of the substance formed when oxygen and haemoglobin react? Where does the reverse of the above reaction take place? What are the three blood vessels in the body? Platelets help blood to clot Small enough to fit through the membranes of capillaries Shaped so that it has a large surface area Contains haemoglobin to carry oxygen and carbon dioxide No nucleus to maximise space to carry oxygen and carbon dioxide Carries other chemicals around the body Oxyhaemoglobin In the tissues Arteries Veins Capillaries Arteries transport blood away from the heart Veins transport blood to the heart Capillaries exchanging materials with tissues Arteries have thick muscular and elastic walls Veins have large lumen and values to prevent back flow Capillaries have permeable walls The right side of the heart pumps blood to the lungs What do the three blood vessels in the body do? How are the three blood vessels adapted to their functions? Where does the right side of the heart pump blood to? Where does the left side of the heart pump blood to? Where is blood at higher pressure – veins or arteries? Why does blood flow from one place to another? The left side of the heart pumps blood to the rest of the body Blood is at higher pressure in the arteries Blood flows from areas of high pressure to areas of low pressure What do the left and right ventricles in the heart do? Why does the left ventricle have a thicker muscle wall than the right ventricle? What do the left and right atria in the heart do? They pump blood. The left ventricle has to pump blood around the entire body. The receive blood. What do the semilunar, tricuspid and bicuspid valves do in the heart? What are the four main blood vessels of the heart? They prevent backflow. Aorta Vena cava Pulmonary vein Pulmonary artery Can pump at higher pressure Therefore greater rate of flow to the tissues Contains cell sap and provides support Cellulose to provide support Bacterial cells are simpler and smaller than animal and plant cells. A ‘true’ nucleus Mitochondria Chloroplasts No nucleus in a bacterial cell Circular strand in a bacterial cell and chromosomes in an animal/plant cell As an increase in height As an increase in wet or dry mass Dry mass One just after birth One in adolescence Cell division then cells becoming specialised Cell differentiation Stem cells Embryonic tissue Different cells, tissues and organs Adult stem cells can only become certain cells. Embryonic stem cells can become all types of body cell Animals only grow in the early stages of their lives and reach a finite size Plants grow continually All parts of an animal are involved in growth Plants grow at specific parts of the plant. Meristems What is the advantage of the double circulatory system in mammals? What is the function of the vacuole in a plant cell? What is the cell wall made of? What is the difference between bacterial and animal and plants cells? What do bacterial cells lack? How is the arrangement of the DNA in a bacterial cell and an animal/plant cell different? How can growth be measured? What is the best measurement of growth? When are the two rapid phases of growth in human growth? How can growth be described in terms of cell division? What is the process by which cells become specialised called? What are undifferentiated cells called? Where are stem cells obtained from? What can stem cells develop into? What is the difference between adult and embryonic stem cells? How is animal and plant growth different? What is the name of the areas of plants where cell division happens? What is the main method by which plants gain height? What do many plant cells keep but most animals lose at an early stage? What is the process of selective breeding? Cell enlargement The ability to differentiate Selection of desired characteristics How can selective breeding reduce the gene pool? What is artificially transferring genes from living to another called? Cross breeding individuals with those characteristics Selection of suitable offspring over may generations Inbreeding which can cause health problems within the species Accumulation of harmful recessive characteristics Reduction in variation Genetic engineering or genetic modification Give an advantage of genetic engineering Give a risk involved with genetic engineering Give three examples of genetic engineering Organisms with desired features are produced rapidly Inserted genes may have unexpected harmful effects Inserting the gene from carrots that produces betacarotene into rice so that humans can process betacarotene into vitamin A from rice. Production of human insulin by genetically engineered bacteria Transferring resistance to herbicides, frost damage or disease to crop plants Selection of desired characteristics Isolation of genes responsible Insertion of genes into other organisms Replication of these organisms Change a person’s genes and cure certain disorders Gene therapy Body cells or gametes If genes can be changed to cure disorders before conception, what other genetic characteristics could be changed prior to conception of a baby? What can selective breeding lead to? What is the process of genetic engineering? What might it be possible for genetic engineering to do in the future? What is it called when a person’s genes are changed in an attempt to cure disorders? What type of cells could gene therapy involve? Why is gene therapy involving gametes controversial?