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Intermediate 2 Revision notes Section 1 Introduction to cells Cells are the living units from which all organisms are made. Simple organisms have only one cell e.g. bacteria they are called unicellular Other organisms have many cells working together they are called multicellular e.g. humans This is the structure of an animal cell cell membrane allows certain substances into or out of the cell nucleus cytoplasm controls all activities in the cell chemical reactions take place here Iodine is used to stain a cheek cell. Stains make certain cell structures show up more clearly. This is the structure of a plant cell fluid filled sac stores water and minerals nucleus vacuole cell wall made of cellulose provides support for cell cytoplasm green chloroplast cell membrane contains chlorophyll traps light energy in photosynthesis Functions of parts of the cell structure nucleus cell membrane cytoplasm cell wall vacuole chloroplast feature contains genetic material function controls cell activities thin layer surrounding controls entry and cytoplasm exit of materials fluid jelly like material cellulose outer layer in plants only site of chemical reactions fluid filled sac in cytoplasm stores water and solutes contains chlorophyll traps light energy supports cell • cell wall Only plant cells contain : • chlorophyll • vacuole The cell wall and vacuole provide support to the cell. Plant cells with many chloroplasts would be found in parts of a plant which produces food by photosynthesis. Plant cells are rigid and have a fixed shape. Animal cells are flexible and can vary their shape. Comparing plant and animal cells nucleus Found in both plant and animal cells. cytoplasm Found in both plant and animal cells. membrane Found in both plant and animal cells. cell wall Only found in plant cells. vacuole Only found in plant cells. chloroplast Only found in plant cells. A microbe is a microscopic one-celled organism Examples of microbes are: bacteria amoeba yeast • Microbes are found almost anywhere that life exists. • Some are harmful because they can cause disease and rot food. • Others are useful for wine making, recycling nutrients in nature, and in biotechnology. bacteria •Bacteria feed on the milk sugar lactose. •The lactose is converted into lactic acid during this process. •The lactic acid changes the milk into yoghurt. yeast One yeast cell [Very small. Not much detail] Yeast is a unicellular fungus. The cells are not mobile. Not much detail is seen in the cells. The cells are smaller than plant or animal cells. Fermentation yeast + - Biological process by which yeast changes sugar into alcohol and carbon dioxide. sugar alcohol + carbon dioxide Humans have made use of 3 yeast industries for thousands of years. •Wine making •Baking •Brewing Uses of Yeast (1) Wine Making yeast + fruit sugar alcohol + carbon dioxide •Wines, beers, spirits for drinking. •Used as a fuel substitute for petrol! Uses of Yeast (2) Baking yeast + sugar carbon dioxide carbon dioxide makes dough rise + alcohol alcohol evaporates when dough is cooked Uses of Yeast (3) Brewing yeast + malted barley + hops Starch in barley changes to sugar added for flavour alcohol + carbon dioxide Antibiotics •Alexander Fleming discovered penicillin •Penicillin is an antibiotic •Antibiotics come from fungi •Antibiotics kill bacteria Antibiotics do not kill viruses (colds & flu are viruses) Some bacteria are now resistant to antibiotics Diffusion This is the natural movement of particles from an area of high concentration to an area of low concentration until they are evenly spread. A purple crystal is added to a beaker of water. purple dye spreads evenly through water water star t purple crystal after 15 mins molecules of the purple crystal move from an area of high concentration (the crystal) to an area of low concentration (the water) this is an example of diffusion The process of diffusion purple dye spreading out •The water molecules are moving all the time •The movement of the water molecules causes the crystal molecules to spread out •Diffusion will stop when the crystal molecules are evenly spread throughout the water Diffusion allows useful substances to pass into a cell high concentration of oxygen low concentration of CO2 high concentration of glucose And waste substances to pass out of a cell In multicellular organisms e.g. humans,oxygen cannot diffuse into the cells directly from the air, so there must be a method to take the oxygen to the cells. •We breathe in air which has 20% oxygen in it •The oxygen diffuses into the blood vessels in the lungs •The blood carries the oxygen to all body cells •Oxygen diffuses into the cells from the blood Cells use the oxygen and glucose to produce energy. glucose + oxygen energy + CO2 + water This process is called respiration. energy This is used up by the cell. CO2 This diffuses out of the cell into the blood and is taken to the lungs to be removed. water This diffuses into the blood and is taken to the kidneys to be removed. •Substances enter and leave cells by diffusion •They diffuse through the cell membrane •Substances diffuse from a high concentration to a low concentration •Diffusion stops when molecules are evenly concentrated Osmosis is a special type of diffusion osmosis Diffusion of water from an area of high water molecule concentration to an area of low water molecule concentration across a selectively permeable membrane. A selectively permeable membrane only allows small molecules to pass through it.It does not allow large molecules to pass through. The difference in concentration between 2 solutions is called a concentration gradient. membrane sugar molecule The water diffuses down the concentration gradient water molecule area of high water molecule concentration area of low water molecule concentration water molecules diffuse by osmosis from the area of high water molecule concentration to the area of low water molecule concentration. This shows a visking tubing experiment with sugar solutions of different concentrations. (99.5% water) 0.1% sugar solution H2O 0.5% sugar solution H2O H2O (99.9% water) H2O visking tubing Water will move out of the bag by osmosis and the bag will lose mass. • Water enters and leaves cells by osmosis • A selectively permeable membrane lets some substances through but not others • A concentration gradient exists between 2 solutions of different concentrations of the same substance • Osmosis is special diffusion of water from a high concentration to a low concentration through a selectively permeable membrane • A 5% sugar solution contains 5% sugar and 95% water Osmosis can be explained in terms of water concentrations A hypotonic solution has a higher water concentration than that inside the plant cell. area of higher water concentration hypotonic solution plant cell area of lower water concentration water molecules enter the cell by osmosis An isotonic solution has the same water concentration as that inside the plant cell. water concentrations are the same inside and outside the cell isotonic solution plant cell the same number of water molecules enter the cell as leave the cell A hypertonic solution has a lower water concentration than that inside the plant cell. area of higher water concentration area of lower water concentration hypertonic solution plant cell water molecules leave the cell by osmosis Osmosis in plant cells hypotonic solution (H2O) potato cylinder hypertonic solution (sugar solution) •Each potato was weighed and the mass recorded •Potatoes were left soaking for 15 minutes •After 15 minutes potatoes were blotted dry and weighed •The texture of each potato was felt hypotonic solution (H2O) H2 O potato cylinder H2O H2O •water moves into cell •cells become turgid •mass of cell increases hypertonic solution (sugar solution) H2O H2O H2O •water leaves cell •cells become flaccid •mass of cell increases Potato cells and osmosis nucleus cytoplasm cell membrane cell wall Potato cell in normal healthy condition is described as turgid. Potato cells in hypotonic solution vacuole is swollen due to uptake of water by osmosis cytoplasm is forced against cell wall by pressure from vacuole cell wall is stretched to its maximum Cell is swollen to its maximum size and is said to be fully turgid. Potato cells in hypertonic solution vacuole shrinks due to loss of water by osmosis cytoplasm is pulled away from cell wall because of reduced pressure from vacuole cell wall is unstretched The process of extreme water loss resulting in this situation is called plasmolysis. Plasmolysed cells are soft and limp. They are said to be flaccid. Osmosis in animal cells water enters cell Animal cells in hypotonic solution •water enters by osmosis •cell membrane stretches and bursts cell membrane bursts water leaves cell Animal cells in hypertonic solution •water leaves by osmosis •cell loses water and shrinks cell membrane shrinks This is the end of Section 1 Introduction to cells