Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Histology D502 11.22.04 The Eye Outline: I. Introduction II. Structure of the eye III. Genesis of the eye IV. Sclera V. Uvea VI. Lens VII. Retina VIII. Conjunctiva, Eyelids and Glands associated with the eye I. Introduction: a) organ collects light of various intensity and color b) translates this information into neural signals c) signals processed to give image d) depth of field conferred via binocular vision II. Structure of the Eye (tunics and others): a) Sclera (corneosclera): i) fibrous coat over most of the eye ii) cornea over anterior eye b) Uvea (vascular layer): i) choroid ii) ciliary body iv) iris c) Retina: i) pigmented epithelium (retinal pigmented epithelium) ii) photosensitive retina proper (neural retina) d) Lens e) Internal, liquid filled, chambers: i) anterior chamber: between cornea and iris ii) posterior chamber: between iris and lens iii) vitreous body: between lens and retina, 99% water, remainder salts, collagen and hyaluronic acid (very hydrated) III. Genesis of the eye: a) obvious development by day 22 in the embryo and made of neuroectoderm, surface ectoderm, and mesoderm b) neuroectoderm develops into brain and forebrain outgrowth occurs giving the optic stalk and optic vesicle, invagination of vesicle occurs forming the double layered optic cup i) inner layer develops into the neural retina ii) outer layer develops into the retinal pigmented epithelium, pigmented epithelium of iris and ciliary body, and dilator and sphincter muscles for the pupil (within iris) c) surface ectoderm invaginates within the optic cup: i) this initially forms the lens vesicle that pinches off and develops into the lens ii) the contiguous surface ectoderm develops into the corneal epithelium and the lining of the eyelids d) the mesoderm gives rise to: i) stroma of sclera and cornea ii) uvea: stroma of choroid, ciliary body, and iris IV. Sclera (outermost layer) a) posterior 5/6 covered with opaque connective tissue capsule containing flat collagen bundles and fibroblasts (sclera proper) that approximates 3 layers: i) episclera: outer most layer in contact with eye socket made of loose connective tissue ii) sclera proper (Tenon’s capsule): middle layer, dense network of collagen fibers, tendons of extraocular muscles attach to Tenon’s capsule iii) lamina fuscia: inner layer, adjacent to choroid, made of collagen and elastic fibers and contains pigmented cells b) anterior 1/6 covered with the cornea i) 5 layers thick: (outer to inner) - epithelium: strat. sq. epith, non-keratinized, 5 - 6 cell layers thick: - rich in sensory nerves - high regenerative capacity - cells turnover is 7 days - Bowman’s membrane: 7 -12 micron thick acellular layer of collagen fibers with little ground substance, provides barrier preventing infection but does not regenerate - stroma: about 60 layers of parallel collagen bundles that cross at right angles giving transparency to the cornea, flattened fibroblasts between layers, ground substance of chondroitin and karatan sulfate, thickest layer of cornea, - Descemet’s membrane: 5 - 10 micron thick basal lamina of corneal endothelial cells, can regenerate and increases in thickness with age - Endothelium: simple squamous epithelia with directional ion transport properties ii) cornea is avascular so receives nutrients via transport across the endothelium from anterior chamber and oxygen via diffusion iii) corneal transparency results from uniform arrangement of collagen fibers and their low light scattering iv) junction between cornea and sclera (corneoscleral junction) called limbus: - transition from clear to opaque collagen coat - highly vascular region - location of the canal of Schlemm V. Uvea (vascular or middle layer) a) 3 parts: i) choroid ii) ciliary body iii) iris b) choroid: largest component, 3 layers (out to in) i) vessel layer: medium sized arteries and veins, loose CT and melanocytes ii) chorocapillary layer: capillaries arranged in one plane, fenestrated type iii) Bruch’s membrane: 3 - 4 micron thick amorphous hyaline membrane that the retinal pigmented epithelia rests upon c) ciliary body: expansion of the stroma of choroid near the lens i) contacts three regions: vitreous body, sclera, posterior chamber/lens ii) contains 2 layers of stroma; a vascular loose CT layer with melanocytes, and a smooth muscle (ciliary muscle) layer - loose CT stroma lined with two layers of columnar cells with the basal layer being pigmented - two bundles of smooth muscle - one stretches the choroid - contraction of the other relaxes tension on lens iii) has projections called ciliary processes - project toward lens - fibers composed of oxytalin fibers extend from these projections and attach to the lens - called zonule fibers - lined with two layers of columnar cells as body - cells joined by tight junctions and desmosomes and actively transport ions from the plasma into the posterior chamber forming the aqueous humor of the internal eye - much lower protein (0.1%) than plasma - intraocular pressure slightly higher than intracranial pressure helping to maintain the shape of the eye and the corresponding arrangement of retractile elements of the eye iv) trabecular meshwork within ciliary body near limbus - aqueous humor produced by the ciliary processes passes from the posterior chamber into the anterior chamber (between iris and lens) - aqueous humor drained from anterior chamber via the trabecular meshwork - from here, the humor passes into the canal of Schlemm that drains into the venous system - there is no direct connection between the canal and trabecula, the humor percolates through the tissue into the canal d) iris: covers lens, regulates amount of light reaching retina (pupil diameter) i) anterior aspect made of vascular, loose CT with interspersed melanocytes, number of melanocytes in this layer determines eye color ii) posterior surface lined with a double layer of pigmented epithelium - absorbs light iii) two muscle masses rest upon the pigmented epithelium and regulate iris opening (pupil diameter) - radially arranged myoepithelial cells form the dilator pupillae muscle between the vascular and pigment layer - sympathetic innervation - concentric smooth muscle bundles at the pupil margin (inner aspect of iris) form the sphincter pupillae muscle - parasympathetic innervation VI. Lens: avascular structure that facilitates image focus, made of 3 components: a) lens capsule: the basement membrane of the epithelial cells that is an homogenous translucent CT matrix rich in glycoprotein i) zonule fibers attach to this around the periphery of the lens b) subcapsular epithelium: single layer of cuboidal cells c) Lens fibers: elongated cells derived from the subcapsular epithelia near the equator of the lens i) as the cells grow and are pushed to the optical axis of the lens, they loose their nuclei ii) at the optical axis, the cells are hexagonal and pack in a highly organized fashion with little intercellular space iii) cells contain few organelles but are high in protein (60 -70%) - major protein is crystallins - function to increase refractive index of cytosol d) accommodation of lens: i) lens thinner when focused on distant objects, relaxed ciliary muscles ii) lens thicker when focusing on near objects, ciliary muscles contract, relaxing tension on zonule fibers thus thickening lens (anterior directed movement of ciliary body) VII. Retina: location of photosensitive cells and neural networks a) vascularized cellular layer b) essentially 4 cell (nuclei) layers (out to in) i) retinal pigmented epithelium ii) photosensitive layer iii) intermediate layer iv) internal layer c) pigment epithelium: i) rest upon Bruch’s membrane of choroid ii) rich in melanin iii) send processes into photosensitive layer - processes contain melanin but the photosensitive cells do not bite-off the melanosomes iv) pigment prevents light scattering after passing through photosensitive layer v) cells involved in recycling vitamin A of photopigments via phagocytosis of shed membrane from photoreceptor cells d) photosensitive layer: 2 cell types i) Rod cells: rod-shaped, light intensity sensors, dark accommodation - elongate cells with inner and outer segment - outer segment contains stacks of membrane containing vit. A bound to rhodopsin - inner segment contains nucleus, cell organelles and synapse with bipolar cells - photons cause isomerization of retinal from 11-cis to all trans while bound to rhodopsin (a trimeric GTP-binding protein linked receptor) - isomerization results in a cascade of events (st) mediated by transducin (the G-protein trimer associated with rhodopsin activation): - activated rhodopsin binds to transducin and weakens the binding of the alpha subunit to the trimeric G protein - the alpha subunit dissociates, binds to cyclic GMP (cGMP) phosphodiesterase and increases the activity of this enzyme - enzyme activation increases the hydrolysis of cGMP, thus decreasing the cellular cGMP - Na+/Ca2+ channels within the membrane with bound cGMP are open, without it is closed - the decreased cGMP results in cGMP dissociating from the cGMP-dependent Na+/Ca2+ channel and close the channel - decreased Na+ permeability results in membrane hyperpolarization thus generating an electrical signal (not depolarization) that is sent to the brain - decreased Ca2+ permeability results in decreased cytosolic Ca2+, Ca2+ dissociates from a protein called recoverin, without Ca2+, recoverin can bind guanyl cyclase and increase the activity of this enzyme - increased guanyl cyclase activity “recovers” the lowered cGMP levels induced by the photons - the increased cytosolic cGMP results in cGMP binding to the cGMP-dependent Na/Ca2+ channel thus re-opening the channel - trans retinal released from rhodopsin - pigment epithelium recycles vit. A ii) Cone cells: cone-shaped outer segment, similar inner segment to rod cells - color sensitive cells - pigment protein is iodopsins - thought to be three different forms for blue, green and red - photochemistry similar to rhodopsin but wavelength specific e) intermediate layer: bipolar cells i) synapse with inner segment of photosensitive cells ii) synapse with ganglion cells of inner layer f) internal layer: ganglion cells i) form synapse with bipolar cells ii) fuse together to form optic nerve and carry information to brain g) additional cells of retina: i) horizontal cells: connect photoreceptor cells, integrative function ii) amacrine cells: contact ganglion cells, conducting cells iii) supporting cell:Muller cells - Muller cells: ramify through the retinal layer (extend the full its full thickness) and form a basement membrane adjacent to the vitreous humor, support function h) specialized structures of retina: i) fovea: depression in which retina is very thin - bipolar and ganglion cells at periphery giving thinness - region devoid of rod cells - region of increased visual acuity at the optical axis ii) optic papilla: - off optical axis - devoid of photosensitive cells - location of exit of optic nerve from eye VIII. The Conjunctiva, Eyelid, and Glands of the eye: a) conjunctiva: a mucous membrane covering the lateral margins of the cornea, the anterior aspect of the sclera and the internal surface of the eyelid: i) stratified squamous (near cornea) to columnar epithelia (other regions) with goblet cells ii) subdivided into: - ocular conjunctiva (that over the orbit) - palpebral conjunctiva (that lining the interior of the eyelid) b) Eyelid: protects the eye i) thin skin on exterior ii) eyelashes at the palpebral (lid to lid) junction with 2 to 3 rows of hair follicles iii) orbicularis oculi skeletal muscle iv) tarsal plate: fibroelastic tissue v) conjunctiva vi) glands within the lids - sebaceous glands for lashes (Glands of Zeis) - sweat glands for epidermis (Glands of Moll) - tear-contributing glands (Meibomian or tarsal glands) see below c) Non-integumental glands of the eye: function to secrete liquids to protect eye surface from dehydration and bug growth (lysozyme) i) tear film: 3 layers - outer lipid layer to prevent evaporation (tarsal glands) - middle aqueous layer (lacrimal gland) - inner mucous layer (goblet cells of conjunctiva) ii) two main glands involved: - tarsal (Meibomian) gland: - located within eyelids on the interior aspect and within the tarsal plate - 20 to 30 per lid - specialized sebaceous gland that secretes oily product onto eye (does not secrete onto hair follicle) - lacrimal gland: - located in the anterior, superior, temporal region of eye socket - tubuloalveolar serous gland - tear secreting gland, lubricates and protects eye epithelium