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DNA and Translation • Gene: section of DNA that creates a specific protein – Approx 25,000 human genes • Proteins are used to build cells and tissue • Protein synthesis involves two processes: 1) Transcription 2) Translation • Transcription takes place in the nucleus – 1) DNA double helix is broken apart – 2) mRNA nucleotides match up – 3) Finished mRNA detaches, and moves to a ribosome • Codon: Combination of 3 mRNA nucleotides • Each mRNA codon matches with 1 of 20 amino acids • Ribosome reads codons 1 at a time • Codon AUG = Methionine (Start) • Codon GUU = Valine • Codons UAA or UAG or UGA = Stop • Defined: Process of making proteins – Step 1: mRNA enters ribosome – Step 2: Ribosome reads one mRNA codon at a time (starting with the mRNA codon AUG) – Step 3: tRNA delivers amino acids until a stop codon (one of 3) is reached = a completed protein. Translation Details • Translation begins when the mRNA codon “AUG” is read by the ribosome • Each mRNA codon matches with a specific amino acid AUG = methionine GCU = alanine • tRNA carries over the proper amino acid – tRNA anticodon matches with the mRNA codon – Ensures proper match • One by one, amino acids are linked together • Translation ends when a “stop” codon is read by the ribosome • End result: Protein NowThis the is codon why and anticodon match. This the anticodon ensures the proper is important! amino acid (serine) is delivered. Because the codon and anticodon don’t match, the wrong amino acid will not be delivered. Questions to answer: 1) List the amino acids that will be delivered to this ribosome. 2) What is the anticodon of each codon? 3) When finished, how many amino acids in size is this protein? Methi onine Proline Serine Proline Glycine Serin e STOP CODON tRNA tRNA tRNA tRNA tRNA tRNA tRNA UAC GGC UCA GGU CCU AGA ACU CCG AGU CCA GGA GAU AUG Ribosome UCU UGA Practice Problem Always use mRNA to find the amino acid DNA mRNA codon tRNA anticodon Amino Acid Practice Problem Always use mRNA to find the amino acid DNA mRNA codon tRNA anticodon Amino Acid Practice Problem Always use mRNA to find the amino acid DNA mRNA codon tRNA anticodon C C A G G U C C A Amino Acid REview 1) Define transcription? Translation? 2) Where does transcription take place? Translation? 3) What are genes and how are they different from chromosomes? 4) How many nucleotides make a codon? 5) The anticodon can be found on which object? 6) The anticodon must match the ____________. 7) Place the following steps in order from start to finish: a. Proteins are assembled b. Ribosome reads a codon one at a time c. mRNA arrives at the ribosome d. tRNA delivers amino acids to the ribosome 7) Be able to solve practice problems similar to the three examples on slides # 9 -11