Download Cellular Respiration and Fermentation Pre

BIOL 1107L
Cellular Respiration and Fermentation
Cellular Respiration and Fermentation
Pre-Lab Objectives
These objectives should be completed and entered into your laboratory notebook before
coming to lab. This information will be helpful for any lab quizzes/exams and is expected to be
in your notebooks during notebook checks.
Introduction
1. Define the following:
Fermentation
Cellular respiration
Adenosine triphosphate (ATP).
2. What is the purpose of ATP in the cell?
3. Differentiate between an aerobic and anaerobic process.
4. Define oxidation and reduction (use the definition involving electrons). Why must oxidation
and reduction occur together?
5. How is NAD+ reduced to NADH? What is the relevance in cellular respiration.
6. List the stages of cellular respiration (glycolysis, Kreb’s cycle, and electron transport chain) in
order. What is the cellular location of each? Which ones require oxygen? What is the purpose
and products of each? (You do not have to do the questions on pp. 105, but they may be
helpful in doing this section.)
7. What is the purpose of fermentation? Under what conditions does fermentation occur in
select cells? What are the end products alcoholic and lactic acid fermentation? What happens
to NADH in each?
8. How many ATP are produced by aerobic respiration? Anaerobic respiration?
Exercise 5.1, Experiment A
9. What organism that is used in this experiment? (common and scientific name).
10. How is the rate of fermentation measured in this experiment? (Think about what byproduct
from fermentation is measured). (You may need to look at Fig. 9.17A, p.178 in your textbook
for this to make sense.)
1
BIOL 1107L
Cellular Respiration and Fermentation
11. What factors (listed in the manual) affect the rate of fermentation? Which one is measured
by this experiment?
12. What is the dependent variable in this experiment?
13. What is the independent variable in this experiment?
14. After studying Table 1 (page 108 of your lab manual), formulate a general hypothesis for
this experiment. (Keep in mind that the hypothesis must be testable using the experiment in
the book.)
15. Predict the outcome for the relative rate of fermentation for the 4 tubes listed in Table 1.
16. What is the purpose of the experiment?
Exercise 5.2, Experiment A
17. Why are we using a mitochondrial suspension for this experiment? What is the source of
the mitochondrial suspension?
18. Which reaction in the Kreb’s cycle are we measuring in this experiment?
19. What is DPIP? What is the source of its electrons?
20. What causes DPIP to change color from Blue to “colorless”? What role does the color
change play in this experiment?
21. How is the DPIP color change measured in this experiment?
22. How does a spectrophotometer works? (We will be measuring percent transmittance.)
23. What is percent transmittance?
24. What is the purpose of a blank when using the spectrophotometer?
25. After studying Table 5.3, formulate a general hypothesis for this experiment. (Keep in mind
that the hypothesis must be testable using the experiment in the book.)
26. State a prediction for the experiment
27. State the purpose of the experiment.
28. What is the dependent variable in this experiment?
29. What is the independent variable in this experiment?
2