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Wayne_sp2017 EKG and Arterial Pulse Pressure EKG Purpose: This exercise is designed to familiarize the student with taking an EKG and observing the phenomenon known as the Mammalian Diving Reflex. Performance Objectives: At the end of this exercise the student should be able to: 1. Take an EKG of another person using the stand limb leads and IWORX equipment. 2. Explain and demonstrate the Mammalian Diving Reflex. 3. Calculate heart rate given the duration of the cardiac cycle. 4. Identify the P, QRS, T wave forms of an EKG. 5. Describe what each wave form of the EKG represents. 6. Calculate a cardiovascular index. Background Activity stimulates the heart to contract more vigorously than it does when the body is at rest. The EKG (electrocardiogram) sensor measures cardiac electrical potential waveforms (voltages) produced by the heart as its chambers contract. The EKG does not directly measure of heart muscle activity. A comparison of the EKG measured during rest and the EKG measured after mild exercise show the changes that take place in the heart cycle due to activity. The Electrocardiogram R P Wave T Wave Q S QRS Complex The part of the EKG (electrocardiogram) before any measurement is taken is called the baseline. The first deviation from the baseline (isoelectric point) in a typical EKG is an upward pulse following by a return to the base line. This is called the P wave and it lasts about 0.04 seconds. 1 Wayne_sp2017 After a return to the baseline there is a short delay while the heart’s AV node depolarizes and sends a signal along the atrioventricular bundle of conducting fibers (Bundle of His) to the Purkinje fibers, which bring depolarization to all parts of the ventricles in a wave that is almost simultaneous. When the AV node depolarizes there is a downward pulse called the Q wave. Shortly after the Q wave there is a rapid upswing of the line called the R wave followed by a strong downswing of the line called the S wave and then a return to the baseline. These three waves together are called the QRS complex. This complex is caused by the depolarization of the ventricles and is associated with the contraction of the ventricles. Next there is an upward wave that then returns to the base line. This upward pulse is called the T wave and indicates repolarization of the ventricles. The sequence from P wave to T wave represents one heart cycle. The number of such cycles in a minute is called the heart rate and is typically 70-80 cycles (beats) per minute at rest. P-R interval... 120-200 milliseconds (0.120 to 0.200 seconds) QRS interval... under 100 milliseconds (under 0.100 seconds) Q-T interval... under 380 milliseconds (under 0.380 seconds) A. Activity: EKG and Finger Pulse: Baseline Recordings The IWorx system will be used to record individual EKG’s and finger pulses. Each student should make a baseline recording of their own EKG and finger pulse. Each student should only use ONE set of electrodes for EKG, do not remove them until you are finished with this exercise 1. The equipment should be set up and preprogrammed for you. 2. Prepare the subject for recording by having them remove all jewelry from wrists and ankles. Then swab the ventral surface of each wrist and an area on the left leg just above the ankle with an alcohol swab. You will only be using three of the leads. 3. Place the plethysmograph (PPG) on the volar surface (area of fingerprint) of the distal segment of the index or thumb finger and wrap the Velcro to attach the unit firmly to the end of the finger. Plethysmograph can be used to record changes in blood volume as the arterial pulse expands and contracts the microvasculature. 4. Have the subject to sit quietly with hands in their lap 5. Click <Record> and, if needed, <AutoScale> in the channel two title area 2 Wayne_sp2017 6. You should see a rhythmic EKG tracing and pulse appear on the screen if the trace is upside down, click <Stop> and switch the positive and negative electrode leads or right click and <invert>. If a cleaner signal is required, the electrodes should be moved from the wrists to the area immediately below each clavicle and the ground next to the umbilicus. 7. When you are getting a suitable trace, type the “’subjects name’ and ‘resting’” and press Enter on the keyboard. 8. Click <stop> to halt recording 9. Proceed to the data analysis section of the exercise 8. Proceed to the data analysis section of the exercise 9. Each student should print a copy of their resting EKG, label the major waves on their EKG and paste one in the indicated space. 10. To save a picture of the EKG: Go to file, export and save as Jpeg on a flash drive or desktop. Email the picture to yourself and then print at home or on the lab printer. If the picture is placed in a word document, it can be resized to fit the indicated space. Place Tracings Here 3 Wayne_sp2017 B. Determining heart rate. 1. Determine the heart rate as follows: Heart rate = (beats / minute) 60 sec./min. time interval between successive intervals in seconds. (R-R interval)* *This is the heart rate at one moment in time, for a more reliable estimation of heart rate take the average of 10, R-R intervals. Heart Rate (EKG) using 1, R-R interval: ________________ Heart Rate (EKG) using 10, R-R intervals: ______________ Using the formula above, calculate heart rate using two peaks from the pressure waves of the PPG, or again for a better estimate use the average of 10 wave forms. Heart Rate (PPG) using 1, R-R interval: _______________ Heart Rate (PPG) using 10, R-R intervals: ______________ 2. Pick one complete cycle (Heart Beat) and calculate the time difference between the generation of a heart beat and the actual pulse (PPG). 1. Press the 2-cursor icon and place one cursor on the peak of the QRS wave in channel1 2. Place the other curser in the peak of the finger pulse in window #2 3. Find the time difference in the T2-T1 window; this is a rough estimate of the time it took for the pressure pulse generated by the heart to reach the artery in your finger Time Difference: _________________ 3. Let the EKG run for approximately 10 seconds and watch for a normal rhythm (If an arrhythmia is present, call your instructor to verify). Also observe the relationship between atrial and ventricular rhythms. _____________________________________________________________ 4 Wayne_sp2017 4. _____________________________________________________________ Record the EKG and pulse of a student lying down after resting quietly for a few minutes. Then have the student stand quickly. Observe differences in the heart rate. Explain. HR (Lying Down): _____________ HR (Standing): ________________ __________________________________________________________________ __________________________________________________________________ __________________________________________________________________ 5. Describe the effect of exercise on the EKG. Make sure the electrodes are securely placed, disconnect the leads from the at the limb electrodes and allow the subject to engage in several minutes of vigorous exercise, preferably by placing one foot on a chair and raising and lowering the body repeatedly. Rapidly return the subject to the supine position; reconnect the limb leads. Why is speed essential in re-connecting the subject to the EKG? _______________________________________________________________ _______________________________________________________________ _______________________________________________________________ C. Calculating Intervals from the EKG 1. Determine the P-Q interval from your EKG and record the time. 2. Determine the Q-T interval from your EKG and record the time. 3. Determine the R-R interval and record the time in the table below. Item P - Q interval Q -T interval R -R interval Rest (sec) Exercise (sec) Compare your values for the P-Q, and Q-T intervals for the EKG at rest to the values for the P-Q, and Q-T intervals for the EKG after mild exercise. How do the time intervals for the EKG after mild exercise compare to the time intervals for the EKG at rest? Explain the differences. 5 Wayne_sp2017 _______________________________________________________________ _______________________________________________________________ _______________________________________________________________ What percent of the time are the ventricles contracting? Use the QT interval to help you with the calculation. ______________________________________ 6 Wayne_sp2017 Part III: The Mammalian Diving Reflex Using a student volunteer, the instructor will demonstrate the Mammalian Diving Reflex A sphygmomanometer, stethoscope, IWORX System alcohol swabs, and volunteer are needed for the test. A pan of ice water, pan of room temperature water and towels are needed, along with all the previously mentioned materials, to demonstrate the diving reflex. Initially, a three lead EKG is performed on a subject that is sitting and resting with their arms resting on their thighs breathing normally. For the diving reflex, the subject is asked to hold their breath and then immerse their entire face (including forehead) into the pan of ice water while continuing to hold their breath as long as possible. The EKG and blood pressure “before and after” results are recorded. Heart Rate at end of breath holding in warm water: _____________ Heart Rate at end of breath holding in cold water: ______________ Part IV: Harvard Step Test INTRODUCTION The Harvard Step Test was developed in the Harvard Fatigue Laboratory in World War II to screen men for physical fitness and to evaluate the progress of physical training programs. The Harvard Step Test measures general endurance or physical condition. The test consists of having the subject step up and down on a bench 20" high (16" for women) for 5 minutes, if possible and then measuring heart rate recovery during a post-exercise period. Subjects should stop if they feel that they cannot continue1. This exercise should only be done by those individuals who are in good physical health. PROCEDURE 1. The subject stands in front of a bench of appropriate height. An observer stands behind the subject. The subject steps up and down on the bench at a rate of 30 steps (all the way up and down constitutes one step) per minute for a maximum of 5 minutes. The observer should watch the subject to insure the subject maintains the correct pace. The observer should also watch the subject to prevent accidental falls. 2. When the subject stops at the end of 5 minutes or sooner if the subject cannot keep pace, the subject should relax while the observer gets ready to take the subject's pulse. The duration of the exercise should also be noted. 7 Wayne_sp2017 3. Taking the Pulse: A. One minute after finishing the test take your pulse rate (bpm)- Pulse 1 B. Two minutes after finishing the test take your pulse rate (bpm) - Pulse 2 C. Three minutes after finishing the test take your pulse rate (bpm) - Pulse 3 4. The physical fitness index is computed from the following formula: Index = 5. duration of the exercise in seconds x 100 --------------------------------------------------2 (Sum of Pulse 1, Pulse 2, Pulse 3) Interpretation of Scores: Gender Excellent Above Average Average Below Average Poor Male >90 80-90 65-79 55-64 <55 Female >86 76-86 61-75 50-60 Table Reference: McArdle W.D. et al; Essential of Exercise Physiology; 2000 6. <50 Record your result or that of someone from the class________________. The Harvard Step Test can be modified to equalize the work for persons of different height. If the equipment is available, use the following adjusted bench heights: HEIGHT BENCH HEIGHT less than 5' 5' to 5' 3" 5' 3" to 5' 9" 5' 9" to 6' greater than 6' 12" 14" 16" 18" 20" Useful References: 1. D. F. Speck, D. S. Bruce, Undersea Biomed. Res. 5(1), 9-14 (1978). 2. C. D. Moyes, P. M. Schulte, Principles of Animal Physiology. (Benjamin Cummings, 2007). 3. S. Colberg, Against all odds, toddler Gore Otteson survives a near-drowning and an hour with no heartbeat (2010). Available at http://newsok.com/against-all-odds-toddler-gore-otteson-survives-a-near-drowning-and-an-hour-with-no-heartbeat/article/3496460 (September 2010). 4. When breathing stopped, the miracle began: 21-month-old Gore Otteson defies odds with recovery from near-drowning (2010). Available at: http://www.gunnisontimes.com/index.php?content=C_news&newsid=6715 (September 2010). 5. J. Blevins, Watery tragedy averted as Lakewood toddler’s life “miraculously” revived (December 2010). Available at: http://www.denverpost.com/news/ci_16852219 8