Download Disease name

OIE Reference Laboratory Reports
Activities in 2011
Name of disease (or topic) for
which you are a designated OIE
Reference Laboratory:
Address of laboratory:
White spot disease
Maricultural Organism Diseases Control &
Molecular Pathology Laboratory
Yellow Sea Fisheries Research Institute,
Chinese Academy of Fishery Sciences
106 Nanjing Road
Qingdao, SD 266071
THE PEOPLE’S REPUBLIC OF CHINA
Tel.:
Phone: +86-532-5823062
Mobile: +86-138-05421513
Fax:
+86-532-5811514
e-mail address:
[email protected]
[email protected]
website:
www.ysfri.ac.cn
Name (including Title and
Position) of Head of Laboratory
(Responsible Official):
Dr Jie Huang, Senior Researcher
Name(including Title and
Position) of OIE Reference
Expert:
Dr Jie Huang, Senior Researcher
Name (including Title and
Position) of writer of this report
(if different from above):
Annual reports of OIE Reference Centres, 2011
Ms Bing Yang, Associate Researcher
Dr Jie Huang, Senior Researcher
1
White spot disease
Part I: Summary of general activities related to the disease
1.
Test(s) in use/or available for the specified disease/topic at your laboratory
Maricultural Organism Diseases Control and Molecular Pathology Laboratory (MODCAMPL) of Yellow Sea
Fisheries Research Institute, Chinese Academy of Fishery Sciences (YSFRI, CAFS) provided diagnosis and
surveillance services for shrimp viral diseases using the recommended methods outlined the Manual of Diagnostic
Tests for Aquatic Animals (OIE 2011) in 2011, as well as has been engaged in the development of new technology
for the diagnosis, surveillance and prevention for shrimp viral diseases. Tests in use for white spot disease included
polymerase chain reaction (PCR), real time PCR (qPCR), histopathology, loop-mediated isothermal amplification
(LAMP) and bioassay.
2.
Test
For
Specificity
Total
PCR
Diagnosis and surveillance
All known WSSV genotypes
51
qPCR
Confirmatory diagnosis and virus
quantification
All known WSSV genotypes
540
Histopathology
Initial diagnosis
Lesions
6
LAMP
Confirmatory diagnosis
All known WSSV genotypes
230
bioassay
Reference material preservation,
virus material preparation, and
disease-preventing probiotics tests
All known WSSV genotypes
16
Production and distribution of diagnostic reagents
MODCAMPL of YSFRI has made a series of field-using high sensitive detection kits for detection of white spot
syndrome virus (WSSV), infectious hepodemal and haematopoitec necrosis virus (IHHNV), Taura syndrome virus
(TSV), yellow head virus (YHV), hepatopancreas parvovirus (HPV), spherical baculovirus (MBV), infectious
myonecrosis virus (IMNV), tetrahedral baculovirus (BP), Penaeus vannamei nodavirus (PvNV), Macrobrachium
rosenbergii nodavirus (MrNV), necrotising hepatopancreatitis bacteria (NHPB), Spiroplasma penaei. The kits
were provided for the shrimp farms, hatcheries, aquaculture disease control stations, some research laboratoriesin
the coastal provinces of China in 2011. The total of 269 field-using high sensitive detection kits for WSSV were
distributed.
Part II: Activities specifically related to the mandate
of OIE Reference Laboratories
3.
International harmonisation and standardisation of methods for diagnostic testing or the
production and testing of vaccines
As a OIE expert for WSD, Dr Jie Huang was invited to review the WSD chapter in the Manual of Diagnostic Tests
for Aquatic Animals (OIE 2011) and provided 28 comments and revisions for 33 location in the chapter including
an addition of a recommend protocol for wet mount diagnostic method.
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Annual reports of OIE Reference Centres, 2011
White spot disease
4.
5.
Preparation and supply of international reference standards for diagnostic tests or vaccines
Date
Contact person,
Institution, Country
Type of material
provided
Quantity of
material
Purpose
7/25/11
Dr Dong-Chun Yan
Ludong University, China
WSSV-infected tissues
30g
Positive infected shrimp
tissues
9/25/11
Dr Hong Liu
CIQ of Shenzhen, China
WSSV-infected tissues
35g
Positive infected shrimp
9/28/11
Dr Riu Liu
IOCAS, China
WSSV-infected tissues
35g
Positive infected shrimp
10/10/11
Dr Chen Sun
Ocean University of China
Purified WSSV preparation
1 tube
Positive control
11/27/11
Dr Hong Liu
CIQ of Shenzhen, China
Polyclonal antibodies for VP24
and VP28 of WSSV
2 tubes
Positive control
Research and development of new procedures for diagnosis and control
LAMP diagnostic method and a relavant field using kit for WSD was developed in MODCAMPL in recent years.
The specificity and sensitivity have been improved and reevaluated in 2011. Moreover, three technologies for kit
shipment and preservation under normal temperature was developed in 2011. Auniform and easy sample
preservation technology under normal temperature for DNA, RNA, and protein in aquatic animal tissue has been
developed and evaluated for diagnostic and surveillance purposes.
The laboratory isolated three probiotics which have the activity to decrease the shrimp mortalityby WSSV
infection by 30—50%. More bioassay testing, oral administration technology, and shrimp immune response
analysis, etc. were carried out in this year. The research on interaction between WSSV and host cellullar receptor
was an on-going topic in the laboratory.
6.
Collection, analysis and dissemination of epizootiological data relevant to international disease
control
Dr Jie Huang was noticed with the epizootiological information for massive shrimp disease outbreak in Vietnam
by Dr Eduardo Leano from NACA in 2011 and rapid disseminated this information to the OIE focal point of
China and the Fisheries Bureau of Ministry of Agriculture.
7.
Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the
pathogen and the disease concerned
Dr Jie Huang and other experts in MODCAMPL were invited to participate in the meetings for the administration
regulation for the international reference laboratories in China and the agreements for facilitating of rapid
international materials and reagents shipment organized by the Veterinary Authority of China in 2011.
The laboratory set up two specific rooms with biosafety separation for the disease diagnostic tests and the kit
production. In order to standardize and enhance the quality assurance management of the laboratory, four staff of
the laboratory was assigned to take the training course for inner examination and verification in this year.
8.
Provision of consultant expertise to OIE or to OIE Member Countries
Mr. Huang, a manager of a diagnostic kit manufacturer in Chinese Taipei visit our laboratory and present their new
products and asked for technical advicesand opinion on the applicability for their equipment on Sept. 21, 2011. Dr
Annual reports of OIE Reference Centres, 2011
3
White spot disease
Jie Huang and two more staff in the laboratory receive the visiting and provide a series of opinions for their
products.
Dr Jie Huang was invited by the diagnostic kits assessment officer Dr Dr François Diaz in OIE to provide the
consultatant expertise for the request from a kit manufacturer in the framework of the OIE Procedure for validation
and certification of diagnostic assays Dr Jie Huang provided the encouraging suggesting and technical opinions
based on the knowledge to the new equipment and kits developed by the manufacturer on Nov. 29, 2011 via email.
As an OIE expert for WSD and an AAC member, Dr Jie Huang was invited to review Dr Lightner’s manuscript for
the 2nd Globle OIE Conference on Aquatic Animal Health. Dr Jie Huang finished the revision and provided the
expertise for the manuscript on Sept. 22, 2011.
9.
Provision of scientific and technical training to personnel from other OIE Member Countries
None in 2011.
10. Provision of diagnostic testing facilities to other OIE Member Countries
Dr Megahed Mohamed, a PhD student at Suez Canal University, Egypt emailed Dr Jie Huang for the help for
diagnostic test materials and technical training for the Mediterranean coast in Egypt. Dr Jie Huang answered the
email immediately and indicate that the lab can provide specific field-using high sensitive detection kits for shrimp
pathogens, histopathology services, and some technological training on disease diagnosis.The PhD student provide
his personal CV and required materials listing and phone calls to Dr Huang. According to his requirement, our
laboratory sent kits samples for WSSV and TSV to FedEx in Beijing for the safety identification for international
shipment. The Identification and Classification Reports for Air Transport of Goods issued by the Beijing DGM
Air Transport Technology Co. Ltd Transport Hazard Inspection Laboratory were just received. We are preparing
to air-mail the kits to him.
11. Organisation of international scientific meetings on behalf of OIE or other international bodies
The laboratory organised an opening ceremony for the designation of OIE Reference Laboratories for WSD and
IHHN in Aug. 2011. The President of the Aquatic Animal Health Standards Commission of the OIE, the OIE
Delegate of China, and the officials from the Veternary Authority, the Fisheries Bureau of Ministry of Agriculture,
the General Administration of Quality Supervision, Inspection and Quarantine, and other governmental and local
organisations attended the ceremony. A workshop on the knowledge of OIE was organised after the ceremony. Dr
Barry Hill, the President of AAC presented the presentationsof ‘the OIE Aquatic Animal Health Standards’ and
‘OIE Reference Laboratories’. Dr Jie Huang, as a member of AAC, prsented the presentation of ‘Approaches for
the application of OIE standards for aquatic animal health in Asia’.
12. Participation in international scientific collaborative studies
Dr Qing-Gang Xue, a scientist in Department of Veterinary Science, Louisiana State University, USA was invited
to visit the laboratory as a visiting scholar for one month collaboration on aquatic animal health during July – Aug.
2011. The interaction between WSSV and shrimp cellular receptor was investigated and concluded during his
visiting.
13. Publication and dissemination of information relevant to the work of OIE (including list of
scientific publications, internet publishing activities, presentations at international conferences)

Presentations at international conferences and meetings
Huang, J., The Aquatic Animals Commission and the OIE Aquatic Code. OIE Workshop for Chinese Delegation.
Feb. 2011, OIE Headquarter, Paris, France.
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Annual reports of OIE Reference Centres, 2011
White spot disease
Huang, J., Approaches for the application and development of OIE standards for aquatic animal health in eastern
Asia. OIE Training Workshop for Focal Points in Asian-Pacific, April, 2011, Hu Chi Ming City, Vietnam.
AAC, OIE.Outcomes from OIE General Session (May 2011) and AAC meeting (October 2011) (presented by Dr
Barry Hill, prepared by Dr Jie Huang). NACA AGM10, Nov., 2011, Mangalore, India.
AAC, OIE. Chapter 1.3.Diseases Listed by the OIE.(Due to Indian visa, the presentation was presented by Dr
Barry Hill). NACA AGM10, Nov., 2011, Mangalore, India.
Zhang,Q.-L., Huang, J.*, Li, M.-X., Du, Y.-B., Wang, Q.-T., Yang, B., Song, X.-L.Establishment of the
technological platform for the development of rapid detection kits of aquatic animal pathogens. The 8th
International Symposium on Diseases in Asian Aquaculture, Nov. 2011, Mangalore, India. (Due to Indian visa
problem, the presentation was not presented in the conference).
Liang, Y., Cheng J.-J., Saksmerprome V., Huang J.*. The role of F1-ATP synthase beta subunit in WSSV infection
in the shrimp, Litopenaeus vannamei. The 8th International Symposium on Diseases in Asian Aquaculture (DAA8),
Nov. 2011, Mangalore, India. (Due to Indian visa problem, the presentation was not presented in the conference).

Scientific publications in peer-reviewed journals
Zhang, L., Huang, J., Yang, B.*, Niu, F. 2011. Screening vaccine candidate antigen genes from white spot
syndrome virus (WSSV) by bioinformatics methods. 2011 International Conference on Remote Sensing,
Environment and Transportation Engineering, vol. 8, 7086-7089.
Qiu, Z.-G., Liu, Q.-H.*, Huang, J. 2011. Preferential expression of WSSV-VP37 peptide in E. coli. (Chinese J.)
Progress in Fishery Sciences, 32(2): 96-101.
Qiu, Z.-G., Zhang, Y., Liu, Q.-H.*, Huang, J. 2011. Prokaryotic expression and antibody preparation of white spot
syndrome protein VP24 and VP26. (Chinese J.) Progress in Veterinary Medicine, 32(6): 9-13.
Zhang, Z., Huang, J.*, Gao, Q., Cheng, J.-J., Wang, M.-S. 2011. Genome cloning and sequence analysis of
hepatopancreatic parvovirus (HPV) isolated from Fenneropenaeus Chinensis in China. Journal of Fishery Sciences
of China, 18(1): 59-65.
Li, H.-B., Song, X.-L.*, Wei, S., Liu, J., Li, Y. 2011. Impacts of WSSV on the activity of haemocyte phagocytosis
and hepatopancreas phosphatase in Procambarus clarkia. (Chinese J.) Progress in Fishery Sciences, 32(2): 78-82.
Yu, H.-T., Zhang, X.-L., Huang, J.*, Zhang, S.-C. 2011. The utmost length of PCR product in detection of white
spot syndrome virus (WSSV) using dUTP. (Chinese J.)Progress in Fishery Sciences 32(6): 63-68

Other communications
Answering and providing materials for OIE requests for the short introduction for OIE Bulletin on the OIE RLs for
WSD and IHHN, recommendation of experts and their contacting address to review some diseases chapters of
Aquatic Manual, the photos for the OIE Aquatic Code Cover,
Prepareing the presentation ‘Guidelines for the OIE-NACA Regional Core’ for NACA AGM10, according to the
document made byMs. Simona Forcella. the OIE Animal Health Information Department.
Participating in the national consultation meeting organised by the Chinese Veternary Authority and providing the
comments for OIE Aquatic Manual and OIE Aquatic Code.
Participating in the International Scientific Program Committee of DAA8 and proposing tentative keynote and
invited presentations.
Receiving the short visiting of a delegation of scientists from Belgium and Norway on aquaculture technology.
_______________
Annual reports of OIE Reference Centres, 2011
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