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OIE Reference Laboratory Reports Activities in 2011 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Address of laboratory: White spot disease Maricultural Organism Diseases Control & Molecular Pathology Laboratory Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences 106 Nanjing Road Qingdao, SD 266071 THE PEOPLE’S REPUBLIC OF CHINA Tel.: Phone: +86-532-5823062 Mobile: +86-138-05421513 Fax: +86-532-5811514 e-mail address: [email protected] [email protected] website: www.ysfri.ac.cn Name (including Title and Position) of Head of Laboratory (Responsible Official): Dr Jie Huang, Senior Researcher Name(including Title and Position) of OIE Reference Expert: Dr Jie Huang, Senior Researcher Name (including Title and Position) of writer of this report (if different from above): Annual reports of OIE Reference Centres, 2011 Ms Bing Yang, Associate Researcher Dr Jie Huang, Senior Researcher 1 White spot disease Part I: Summary of general activities related to the disease 1. Test(s) in use/or available for the specified disease/topic at your laboratory Maricultural Organism Diseases Control and Molecular Pathology Laboratory (MODCAMPL) of Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (YSFRI, CAFS) provided diagnosis and surveillance services for shrimp viral diseases using the recommended methods outlined the Manual of Diagnostic Tests for Aquatic Animals (OIE 2011) in 2011, as well as has been engaged in the development of new technology for the diagnosis, surveillance and prevention for shrimp viral diseases. Tests in use for white spot disease included polymerase chain reaction (PCR), real time PCR (qPCR), histopathology, loop-mediated isothermal amplification (LAMP) and bioassay. 2. Test For Specificity Total PCR Diagnosis and surveillance All known WSSV genotypes 51 qPCR Confirmatory diagnosis and virus quantification All known WSSV genotypes 540 Histopathology Initial diagnosis Lesions 6 LAMP Confirmatory diagnosis All known WSSV genotypes 230 bioassay Reference material preservation, virus material preparation, and disease-preventing probiotics tests All known WSSV genotypes 16 Production and distribution of diagnostic reagents MODCAMPL of YSFRI has made a series of field-using high sensitive detection kits for detection of white spot syndrome virus (WSSV), infectious hepodemal and haematopoitec necrosis virus (IHHNV), Taura syndrome virus (TSV), yellow head virus (YHV), hepatopancreas parvovirus (HPV), spherical baculovirus (MBV), infectious myonecrosis virus (IMNV), tetrahedral baculovirus (BP), Penaeus vannamei nodavirus (PvNV), Macrobrachium rosenbergii nodavirus (MrNV), necrotising hepatopancreatitis bacteria (NHPB), Spiroplasma penaei. The kits were provided for the shrimp farms, hatcheries, aquaculture disease control stations, some research laboratoriesin the coastal provinces of China in 2011. The total of 269 field-using high sensitive detection kits for WSSV were distributed. Part II: Activities specifically related to the mandate of OIE Reference Laboratories 3. International harmonisation and standardisation of methods for diagnostic testing or the production and testing of vaccines As a OIE expert for WSD, Dr Jie Huang was invited to review the WSD chapter in the Manual of Diagnostic Tests for Aquatic Animals (OIE 2011) and provided 28 comments and revisions for 33 location in the chapter including an addition of a recommend protocol for wet mount diagnostic method. 2 Annual reports of OIE Reference Centres, 2011 White spot disease 4. 5. Preparation and supply of international reference standards for diagnostic tests or vaccines Date Contact person, Institution, Country Type of material provided Quantity of material Purpose 7/25/11 Dr Dong-Chun Yan Ludong University, China WSSV-infected tissues 30g Positive infected shrimp tissues 9/25/11 Dr Hong Liu CIQ of Shenzhen, China WSSV-infected tissues 35g Positive infected shrimp 9/28/11 Dr Riu Liu IOCAS, China WSSV-infected tissues 35g Positive infected shrimp 10/10/11 Dr Chen Sun Ocean University of China Purified WSSV preparation 1 tube Positive control 11/27/11 Dr Hong Liu CIQ of Shenzhen, China Polyclonal antibodies for VP24 and VP28 of WSSV 2 tubes Positive control Research and development of new procedures for diagnosis and control LAMP diagnostic method and a relavant field using kit for WSD was developed in MODCAMPL in recent years. The specificity and sensitivity have been improved and reevaluated in 2011. Moreover, three technologies for kit shipment and preservation under normal temperature was developed in 2011. Auniform and easy sample preservation technology under normal temperature for DNA, RNA, and protein in aquatic animal tissue has been developed and evaluated for diagnostic and surveillance purposes. The laboratory isolated three probiotics which have the activity to decrease the shrimp mortalityby WSSV infection by 30—50%. More bioassay testing, oral administration technology, and shrimp immune response analysis, etc. were carried out in this year. The research on interaction between WSSV and host cellullar receptor was an on-going topic in the laboratory. 6. Collection, analysis and dissemination of epizootiological data relevant to international disease control Dr Jie Huang was noticed with the epizootiological information for massive shrimp disease outbreak in Vietnam by Dr Eduardo Leano from NACA in 2011 and rapid disseminated this information to the OIE focal point of China and the Fisheries Bureau of Ministry of Agriculture. 7. Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the pathogen and the disease concerned Dr Jie Huang and other experts in MODCAMPL were invited to participate in the meetings for the administration regulation for the international reference laboratories in China and the agreements for facilitating of rapid international materials and reagents shipment organized by the Veterinary Authority of China in 2011. The laboratory set up two specific rooms with biosafety separation for the disease diagnostic tests and the kit production. In order to standardize and enhance the quality assurance management of the laboratory, four staff of the laboratory was assigned to take the training course for inner examination and verification in this year. 8. Provision of consultant expertise to OIE or to OIE Member Countries Mr. Huang, a manager of a diagnostic kit manufacturer in Chinese Taipei visit our laboratory and present their new products and asked for technical advicesand opinion on the applicability for their equipment on Sept. 21, 2011. Dr Annual reports of OIE Reference Centres, 2011 3 White spot disease Jie Huang and two more staff in the laboratory receive the visiting and provide a series of opinions for their products. Dr Jie Huang was invited by the diagnostic kits assessment officer Dr Dr François Diaz in OIE to provide the consultatant expertise for the request from a kit manufacturer in the framework of the OIE Procedure for validation and certification of diagnostic assays Dr Jie Huang provided the encouraging suggesting and technical opinions based on the knowledge to the new equipment and kits developed by the manufacturer on Nov. 29, 2011 via email. As an OIE expert for WSD and an AAC member, Dr Jie Huang was invited to review Dr Lightner’s manuscript for the 2nd Globle OIE Conference on Aquatic Animal Health. Dr Jie Huang finished the revision and provided the expertise for the manuscript on Sept. 22, 2011. 9. Provision of scientific and technical training to personnel from other OIE Member Countries None in 2011. 10. Provision of diagnostic testing facilities to other OIE Member Countries Dr Megahed Mohamed, a PhD student at Suez Canal University, Egypt emailed Dr Jie Huang for the help for diagnostic test materials and technical training for the Mediterranean coast in Egypt. Dr Jie Huang answered the email immediately and indicate that the lab can provide specific field-using high sensitive detection kits for shrimp pathogens, histopathology services, and some technological training on disease diagnosis.The PhD student provide his personal CV and required materials listing and phone calls to Dr Huang. According to his requirement, our laboratory sent kits samples for WSSV and TSV to FedEx in Beijing for the safety identification for international shipment. The Identification and Classification Reports for Air Transport of Goods issued by the Beijing DGM Air Transport Technology Co. Ltd Transport Hazard Inspection Laboratory were just received. We are preparing to air-mail the kits to him. 11. Organisation of international scientific meetings on behalf of OIE or other international bodies The laboratory organised an opening ceremony for the designation of OIE Reference Laboratories for WSD and IHHN in Aug. 2011. The President of the Aquatic Animal Health Standards Commission of the OIE, the OIE Delegate of China, and the officials from the Veternary Authority, the Fisheries Bureau of Ministry of Agriculture, the General Administration of Quality Supervision, Inspection and Quarantine, and other governmental and local organisations attended the ceremony. A workshop on the knowledge of OIE was organised after the ceremony. Dr Barry Hill, the President of AAC presented the presentationsof ‘the OIE Aquatic Animal Health Standards’ and ‘OIE Reference Laboratories’. Dr Jie Huang, as a member of AAC, prsented the presentation of ‘Approaches for the application of OIE standards for aquatic animal health in Asia’. 12. Participation in international scientific collaborative studies Dr Qing-Gang Xue, a scientist in Department of Veterinary Science, Louisiana State University, USA was invited to visit the laboratory as a visiting scholar for one month collaboration on aquatic animal health during July – Aug. 2011. The interaction between WSSV and shrimp cellular receptor was investigated and concluded during his visiting. 13. Publication and dissemination of information relevant to the work of OIE (including list of scientific publications, internet publishing activities, presentations at international conferences) Presentations at international conferences and meetings Huang, J., The Aquatic Animals Commission and the OIE Aquatic Code. OIE Workshop for Chinese Delegation. Feb. 2011, OIE Headquarter, Paris, France. 4 Annual reports of OIE Reference Centres, 2011 White spot disease Huang, J., Approaches for the application and development of OIE standards for aquatic animal health in eastern Asia. OIE Training Workshop for Focal Points in Asian-Pacific, April, 2011, Hu Chi Ming City, Vietnam. AAC, OIE.Outcomes from OIE General Session (May 2011) and AAC meeting (October 2011) (presented by Dr Barry Hill, prepared by Dr Jie Huang). NACA AGM10, Nov., 2011, Mangalore, India. AAC, OIE. Chapter 1.3.Diseases Listed by the OIE.(Due to Indian visa, the presentation was presented by Dr Barry Hill). NACA AGM10, Nov., 2011, Mangalore, India. Zhang,Q.-L., Huang, J.*, Li, M.-X., Du, Y.-B., Wang, Q.-T., Yang, B., Song, X.-L.Establishment of the technological platform for the development of rapid detection kits of aquatic animal pathogens. The 8th International Symposium on Diseases in Asian Aquaculture, Nov. 2011, Mangalore, India. (Due to Indian visa problem, the presentation was not presented in the conference). Liang, Y., Cheng J.-J., Saksmerprome V., Huang J.*. The role of F1-ATP synthase beta subunit in WSSV infection in the shrimp, Litopenaeus vannamei. The 8th International Symposium on Diseases in Asian Aquaculture (DAA8), Nov. 2011, Mangalore, India. (Due to Indian visa problem, the presentation was not presented in the conference). Scientific publications in peer-reviewed journals Zhang, L., Huang, J., Yang, B.*, Niu, F. 2011. Screening vaccine candidate antigen genes from white spot syndrome virus (WSSV) by bioinformatics methods. 2011 International Conference on Remote Sensing, Environment and Transportation Engineering, vol. 8, 7086-7089. Qiu, Z.-G., Liu, Q.-H.*, Huang, J. 2011. Preferential expression of WSSV-VP37 peptide in E. coli. (Chinese J.) Progress in Fishery Sciences, 32(2): 96-101. Qiu, Z.-G., Zhang, Y., Liu, Q.-H.*, Huang, J. 2011. Prokaryotic expression and antibody preparation of white spot syndrome protein VP24 and VP26. (Chinese J.) Progress in Veterinary Medicine, 32(6): 9-13. Zhang, Z., Huang, J.*, Gao, Q., Cheng, J.-J., Wang, M.-S. 2011. Genome cloning and sequence analysis of hepatopancreatic parvovirus (HPV) isolated from Fenneropenaeus Chinensis in China. Journal of Fishery Sciences of China, 18(1): 59-65. Li, H.-B., Song, X.-L.*, Wei, S., Liu, J., Li, Y. 2011. Impacts of WSSV on the activity of haemocyte phagocytosis and hepatopancreas phosphatase in Procambarus clarkia. (Chinese J.) Progress in Fishery Sciences, 32(2): 78-82. Yu, H.-T., Zhang, X.-L., Huang, J.*, Zhang, S.-C. 2011. The utmost length of PCR product in detection of white spot syndrome virus (WSSV) using dUTP. (Chinese J.)Progress in Fishery Sciences 32(6): 63-68 Other communications Answering and providing materials for OIE requests for the short introduction for OIE Bulletin on the OIE RLs for WSD and IHHN, recommendation of experts and their contacting address to review some diseases chapters of Aquatic Manual, the photos for the OIE Aquatic Code Cover, Prepareing the presentation ‘Guidelines for the OIE-NACA Regional Core’ for NACA AGM10, according to the document made byMs. Simona Forcella. the OIE Animal Health Information Department. Participating in the national consultation meeting organised by the Chinese Veternary Authority and providing the comments for OIE Aquatic Manual and OIE Aquatic Code. Participating in the International Scientific Program Committee of DAA8 and proposing tentative keynote and invited presentations. Receiving the short visiting of a delegation of scientists from Belgium and Norway on aquaculture technology. _______________ Annual reports of OIE Reference Centres, 2011 5