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The Cutting of pGLO plasmid by Restriction Enzymes and Gel Electrophoresis NAME Team Investigation Lab Report 12/6/13 Example paper in Journal of Immunology format The Cutting of pGLO plasmid by Restriction Enzymes and Gel Electrophoresis Introduction Restriction enzymes and gel electrophoresis are an important part of the biological research field. Restriction enzymes cut the DNA backbone at specific nucleotide sequences. The data collected from this cutting can be used to support a variety of different conclusions within a research project. Often times, the restriction enzymes help to determine whether or not certain genes are present within a DNA fragment, plasmid, etc. For example, an experiment was conducted to track Listeria Monocytogenes transmission in the cold smoked fish production chain. With the help of restriction enzymes, EcoRI and PvuII, the scientists were able to support their conclusion that the L. Monocytogenes can persist throughout the salmon production system and may even be linked to human listeriosis cases (Klaebo et al., 2006). Many times, after cutting DNA with restriction enzymes, the next step is to subject the DNA to agarose gel electrophoresis in order to determine the lengths of the resulting DNA fragments. This is done in many lab experiments. For example, scientists were able to determine the role of human pepsins in acid reflux disease. They used agar gel electrophoresis in order to visualize the zones of activity of aspartic proteinases (Roberts, 2006). Each in-text citation must have a corresponding citation in the reference list Label citation list “References” References Include all authors in the References List Klaeboe, H., Rosef, O., Fortes, E., Wiedmann, M., 2006. Ribotype diversity of Listeria monocytogenes isolates of two salmon processing plants in Norway. Int. J. Environ. Health Res. 16, 375-393. Roberts, N.B., 2006. Review article: Human pepsins – their multiplicity, function and role in reflux disease. Aliment. Pharmacol. Ther. 24, 2-9.