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The Cutting of pGLO plasmid by Restriction Enzymes and Gel Electrophoresis
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Team Investigation Lab Report
12/6/13
Example paper in Journal of Immunology format
The Cutting of pGLO plasmid by Restriction Enzymes and Gel Electrophoresis
Introduction
Restriction enzymes and gel electrophoresis are an important part of the biological research field. Restriction enzymes cut the DNA backbone at specific nucleotide sequences. The
data collected from this cutting can be used to support a variety of different conclusions within a
research project. Often times, the restriction enzymes help to determine whether or not certain
genes are present within a DNA fragment, plasmid, etc. For example, an experiment was conducted to track Listeria Monocytogenes transmission in the cold smoked fish production chain.
With the help of restriction enzymes, EcoRI and PvuII, the scientists were able to support their
conclusion that the L. Monocytogenes can persist throughout the salmon production system and
may even be linked to human listeriosis cases (Klaebo et al., 2006). Many times, after cutting
DNA with restriction enzymes, the next step is to subject the DNA to agarose gel electrophoresis
in order to determine the lengths of the resulting DNA fragments. This is done in many lab experiments. For example, scientists were able to determine the role of human pepsins in acid reflux disease. They used agar gel electrophoresis in order to visualize the zones of activity of aspartic proteinases (Roberts, 2006).
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References
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Klaeboe, H., Rosef, O., Fortes, E., Wiedmann, M., 2006. Ribotype diversity of Listeria monocytogenes isolates of two salmon processing plants in Norway. Int. J. Environ. Health Res. 16,
375-393.
Roberts, N.B., 2006. Review article: Human pepsins – their multiplicity, function and role in reflux disease. Aliment. Pharmacol. Ther. 24, 2-9.