Download Automatic Classification of tissue samples

Using Ensemble Models in
the Histological Examination
of Tissue Abnormalities
M. Coakley, G. Crocetti, P. Dressner, W. Kellum, T. Lamin
The Michael L. Gargano 12th Annual Research Day
Friday, May 2nd, 2014
Objective
The objective of this study is:
1. to investigate the possibility of automatically
identifying abnormalities in tissue samples
through the use of an ensemble model on data
generated by histological examination
2. to minimize the number of false negative cases.
Introduction
 As part of breast cancer prevention screening if a lump is found a fineneedle aspiration biopsy (FNAB) is performed.
 Normally the sample is analyzed visually by a pathologist that looks for
cancerous tissues with abnormal characteristics.
 This procedure is time consuming
 Automatic procedures do exists that evaluate cytology features derived
from a digital scan of breast FNAB slides.
 These procedure achieve very high accuracies, and better than manual
procedure, but still have a certain level of false negative
 Our goal is to reduce the false negative rate
The Data
 Wisconsin Breast Cancer Dataset
 Containing 569 samples classified as “normal” or “abnormal”
 12 attributes
 Dataset split:
 Training set: 448 samples.
 Test set: 121 samples.
The Data Cont.…
 Table Structure (12 fields)
Id
Diagnosis (A=Abnormal/ N=Normal)
Radius (mean of distances from center to points on the perimeter)
Texture (standard deviation of gray-scale values)
Perimeter
Area
Smoothness (local variation in radius lengths)
Compactness (perimeter^2 / area - 1.0)
Concavity (severity of concave portions of the contour)
Concave points (number of concave portions of the contour)
Symmetry
Fractal dimension ("coastline approximation" - 1)
Exploratory Data Analysis
The data set was of very good quality
No missing values
Outliers detected through the use of Z-Score, with a
possible outlier falling outside of the interval [-4,+4]
𝑥∗
𝑥 − 𝜇𝑥
=
𝜎𝑥
We detected some outliers, but further investigation
excluded errors in the data.
Exploratory Data Analysis (Cont.…)
 Normality Assumption: variables normally distributed within
acceptable variations
 Skewness within [-2,+2]
 Kurtosis within [-2,+2]
Exploratory Data Analysis (Cont.…)
Normalization: to avoid that variables will influence
the model due to their scales we normalized the
data using the min-Max transformation
𝑥 − 𝑚𝑖𝑛𝑥
𝑥 =
𝑚𝑎𝑥𝑥 − 𝑚𝑖𝑛𝑥
∗
All resulting variables were within the interval of [0,1]
Exploratory Data Analysis (Cont.…)
 Normalization: to avoid that variables will influence the
model due to their scales we normalized the data using the
min-Max transformation
𝑥∗
𝑥 − 𝑚𝑖𝑛𝑥
=
𝑚𝑎𝑥𝑥 − 𝑚𝑖𝑛𝑥
 All resulting variables were within the interval of [0,1]
 Correlation
 We kept radius and dropped the other variables.
Clustering
 Derived a new “cluster” variable by applying the KMeans algorithm with k=2.
Modeling
 Due to the characteristics of the data we applied two
algorithms
 CART (with misclassification costs)
 Logistic Regression
 Confusion Matrixes & Error Rates
Ensemble Model
 We leveraged the confidence interval measures
produced by these models.
 Applied a voting scheme in which the prediction with
the highest confidence wins.
𝑂𝑣𝑒𝑟𝑎𝑙𝑙 𝑒𝑟𝑟𝑜𝑟 𝑟𝑎𝑡𝑒 =
4+1
= 0.04 = 4%
121
𝐹𝑎𝑙𝑠𝑒 𝑛𝑒𝑔𝑎𝑡𝑖𝑣𝑒 𝑟𝑎𝑡𝑒 =
𝐹𝑎𝑙𝑠𝑒 𝑝𝑜𝑠𝑖𝑡𝑖𝑣𝑒 𝑟𝑎𝑡𝑒 =
1
= 0.01 = 1%
77
4
= 0.9 = 9%
44
Conclusions
 The voting-based ensemble model derived through the combination of
decision trees and logistic regression proved to be a very efficient way of
helping in improving the detection of abnormal biopsy samples.
 The very low false negative rate of 1% is a clear indication that this problem
can be solved by the generation of high quality classification solutions,
representing an improvement when compared to other classification
systems developed in the past.
References
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Schnitt, J. L. Connolly, C. A. Gatsonis, and B. J. McNeil, Fine-Needle Aspiration Biopsy of
Nonpalpable Breast Lesions in a Multicenter Clinical Trial, Radiology, 2001, Vol. 219, Issue 3,
pp. 785-792
 W. H. Wolberg, W. N. Street, O. L. Mangasarian, Breast Cytology Diagnosis Via Digital
Image Analysis, Dept. of Surgery, Universit of Wisconsin, 1993
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