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Vaccination with CD20 peptides induces a biologically
active, specific immune response in mice
by Wendy K. Roberts, Philip O. Livingston, David B. Agus, Javier Pinilla-Ibarz,
Andrew Zelenetz, and David A. Scheinberg
Blood
Volume 99(10):3748-3755
May 15, 2002
©2002 by American Society of Hematology
Serologic responses using different adjuvants.Mice were vaccinated with the huCD20-KLH
conjugate and the different adjuvants noted in the figure.
Wendy K. Roberts et al. Blood 2002;99:3748-3755
©2002 by American Society of Hematology
Serologic response to different peptide conjugates or peptide alone with QS21.Mice were
vaccinated with the indicated conjugates or KLH only.
Wendy K. Roberts et al. Blood 2002;99:3748-3755
©2002 by American Society of Hematology
Flow cytometry of CD19+ cells in spleens of vaccinated mice.(A) Flow cytometric analysis of
spleen cells from mice vaccinated with huCD20-KLH, msCD20-KLH, or P190-KLH control
conjugate stained with rat antimouse CD19-FITC antibody.
Wendy K. Roberts et al. Blood 2002;99:3748-3755
©2002 by American Society of Hematology
Serologic responses of the vaccinated mice to CD20+ cells.(A) Staining of Raji cells with B1 antiCD20 monoclonal antibody compared with isotype control (shaded).
Wendy K. Roberts et al. Blood 2002;99:3748-3755
©2002 by American Society of Hematology
Complement-mediated killing of Ramos cells with serum from a mouse vaccinated with huCD20avidin conjugate.Serum plus complement was serially diluted as shown along the X-axis.
Wendy K. Roberts et al. Blood 2002;99:3748-3755
©2002 by American Society of Hematology
Proliferation of spleen cells from vaccinated mice in response to various antigens, including
those as measured by incorporation of 3H-thymidine.Splenocytes were from mice vaccinated
with (A) msCD20-KLH and (B) huCD20-KLH.
Wendy K. Roberts et al. Blood 2002;99:3748-3755
©2002 by American Society of Hematology