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Post-Transplant Cyclophosphamide and Sirolimus Are Synergistic in Preventing Rejection and Inducing Stable Mixed Chimerism Independently of Regulatory T Cells. by Courtney Fitzhugh, Matthew M. Hsieh, Oswald Phang, Camille Madison, Leo Luznik, Jonathan Powell, and John F. Tisdale Blood Volume 114(22):3540-3540 November 20, 2009 ©2009 by American Society of Hematology PT-cy and sirolimus are synergistic. 25 to 40 C57BI6 mice received 200cGy TBI, 22-25 × 106 bone marrow cells from BalbC mice, and PT-cy (Cy) 200mg/kg IP 2 days post transplant with or without sirolimus (Sir) 3mg/kg IP for 30 days starting from 1 day before ... Courtney Fitzhugh et al. Blood 2009;114:3540 ©2009 by American Society of Hematology Fourteen days of sirolimus is sufficient to maintain stable mixed chimerism. 25 to 40 C57BI6 mice received 200cGy TBI, 22-25 × 106 bone marrow cells from BalbC mice, and PT-cy (Cy) 200mg/kg IP 2 days post transplant with or without sirolimus (Sir) 3mg/kg IP... Courtney Fitzhugh et al. Blood 2009;114:3540 ©2009 by American Society of Hematology