Download Lesson 5: Enzymes

Lesson 5:
Enzymes
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Competencies
1. describe the components of an
enzyme – (STEM_BIO11/12-Ii-j-17)
2. determine how factors such as pH,
temperature, and substrate affect
enzyme activity- (STEM_BIO11/12 -Ii-j19)
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Youtube Clip: Fun Facts About Enzyme
Guide Questions:
1. What are enzymes?
2. What functions of enzymes in our
body?
3. What are the types of enzymes?
4. How does enzyme work?
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What Are Enzymes?
• Most enzymes are
Proteins (tertiary
and quaternary
structures)
• Act as Catalyst to
accelerates a
reaction
• Not permanently
changed in the
process
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Enzymes
• Are specific for
what they will
catalyze
• Are Reusable
• End in –ase
-Sucrase
-Lactase
-Maltase
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How do enzymes Work?
Enzymes work by
weakening
bonds which
lowers
activation
energy
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Enzymes
Without Enzyme
With Enzyme
Free
Energy
Free energy of activation
Reactants
Products
Progress of the reaction
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Enzyme-Substrate Complex
The substance
(reactant) an
enzyme acts on
is the substrate
Substrate
Joins
Enzyme
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Active Site
• A restricted region of an enzyme
molecule which binds to the substrate.
Active
Site
Substrate
Enzyme
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Induced Fit
• A change in the
shape of an
enzyme’s active
site
• Induced by the
substrate
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Induced Fit
• A change in the configuration of an
enzyme’s active site (H+ and ionic
bonds are involved).
• Induced by the substrate.
Active Site
substrate
Enzyme
induced fit
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What Affects Enzyme Activity?
• Three factors:
1. Environmental Conditions
2. Cofactors and Coenzymes
3. Enzyme Inhibitors
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1. Environmental Conditions
1. Extreme Temperature are the most
dangerous
- high temps may denature (unfold)
the enzyme.
2. pH (most like 6 - 8 pH near
neutral)
3. Ionic concentration (salt ions)
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2. Cofactors and Coenzymes
• Inorganic substances (zinc, iron) and
vitamins (respectively) are sometimes need
for proper enzymatic activity.
• Example:
Iron must be present in the quaternary
structure - hemoglobin in order for it to
pick up oxygen.
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Two examples of Enzyme
Inhibitors
a. Competitive inhibitors: are
chemicals that resemble an
enzyme’s normal substrate and
compete with it for the active
site.
Substrate
Competitive inhibitor
Enzyme
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Inhibitors
b.
Noncompetitive inhibitors:
Inhibitors that do not enter the
active site, but bind to another part
of the enzyme causing the enzyme to
change its shape, which in turn
alters the active site.
Substrate
active site
altered
Enzyme
Noncompetitive
Inhibitor
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Enzymes are used all over your body!
http://en.wikipedia.org/wiki/File:Digestive_system_diagram_en.svg
Life Sciences-HHMI Outreach. Copyright 2009 President and Fellows of Harvard College
http://upload.wikimedia.org/wikipedia/commons/thumb/6/6f/Major_digestive_enzymes.png/750px-Major_digestive_enzymes.png
Life Sciences-HHMI Outreach. Copyright 2009 President and Fellows of Harvard College
Application:
Sketch an enzyme
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