Download Powerpoint - Blood Journal

Generation of T cells from adult human hematopoietic
stem cells and progenitors in a fetal thymic organ culture
system: stimulation by tumor necrosis factor-α
by Steven F. A. Weekx, Hans W. Snoeck, Fritz Offner, Magda De Smedt, Dirk R. Van
Bockstaele, Griet Nijs, Marc Lenjou, Adriaan Moulijn, Inez Rodrigus, Zwi N.
Berneman, and Jean Plum
Blood
Volume 95(9):2806-2812
May 1, 2000
©2000 by American Society of Hematology
Sorting criteria for CD34++CD38− , CD34+CD38+, and CD34+Lin− cells.(A) Side (SSC) versus
forward (FSC) scatter for ABM mononuclear cells.
Steven F. A. Weekx et al. Blood 2000;95:2806-2812
©2000 by American Society of Hematology
Scatter profile of sorted ABM CD34+ cells.SSC versus FSC at day 0 (A) and after a 36-hour
preincubation with IL-3 + SCF + TNF-α (B).
Steven F. A. Weekx et al. Blood 2000;95:2806-2812
©2000 by American Society of Hematology
Kinetic analysis of the development of ABM CD34+Lin− cells in hybrid FTOC.CD34+Lin− ABM
cells were sorted and cultured together with mouse thymic lobes.
Steven F. A. Weekx et al. Blood 2000;95:2806-2812
©2000 by American Society of Hematology
Effect of IL-3 + SCF and IL-3 + SCF + TNF- on the expression of CD34 and CD127.CD34+Lin−
cells were analyzed at day 0 immediately after cell sorting and after a preincubation period of 36
hours.
Steven F. A. Weekx et al. Blood 2000;95:2806-2812
©2000 by American Society of Hematology