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Consulta: subjectFacets:"Enzymes" Registros recuperados: 141 Data/hora: 09/06/2017 10:45:24 A brief review of nutritional studies for Penaeus monodon Provedor de dados: 5 Autores: Liao, I; Liu, F. In order to develop effective formulated for Penaeus monodon , sufficient information is needed on its nutritional requirements. Unfortunately, research on this area has been very limited. This paper outlines the results of such research. Since data on the nutritional requirements of P. japonicus are relatively well established, some of these data are also reviewed in this paper and are used as a basis for assessing the nutritional requirements of P. monodon . Protein, lipid, carbohydrate, mineral and vitamin requirements are evaluated from the parameters of growth survival and feed efficiency. Studies on the effects of enzymes and the development of microparticulate diet for larvae are also summarized. Tipo: Text Palavras-chave: Penaeus monodon; Pond culture; Shrimp culture; Amino acids; Fatty acids; Marine crustaceans; Diets; Enzymes; Feed efficiency; Survival; Growth; Vitamins; Carbohydrates; Lipids; Proteins. Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/acte-1471.pdf Activation of nitrate reductase of cashew leaf by exogenous nitrite Provedor de dados: 61 Autores: Viégas,Ricardo Almeida; Silveira,Joaquim Albenísio Gomes. Nitrate reductase (NR) plays a central role in plant primary metabolism and exhibits complex regulation mechanisms for its catalytic activity. There is controversy in the literature concerning the possible direct effect of NO2- on the stimulation and/or activation of NR activity. The influence of NO2- was studied on the NR activity in the leaves of 30-day-old cashew (Anacardium occidentale L.) seedlings. Addition of NO2- to the reaction mixture containing leaf enzymatic extract resulted in a remarkable increase in NR activity. A trace amount (5 mumol.L-1) of NO2- was required to achieve full NR activity. The in vitro NR-activity showed a steady time-dependent increase when incubated in the presence of only NO3- + NO2-. In contrast, in vitro NR activity was... Tipo: Journal article Palavras-chave: Anacardium occidentale; Enzymes; NADH; Nitrate metabolism; Nitrate reductase. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000100005 Actividad sanguínea de superóxido dismutasa y glutatión peroxidasa en novillas a pastoreo. Provedor de dados: 119 Autores: JARAMILLO, S.; VILLA, N.A.; PINEDA, A.F.; GALLEGO, A.B.; TABARES, P.; CEBALLOS, A. El objetivo de este estudio fue determinar el balance metabólico nutricional de Cu, Zn, Fe, Mn y Se, mediante la actividad sanguínea de SOD y GSH-Px y establecer la relación entre la concentración de Cu, Fe, Mn y Zn en el forraje y la actividad de SOD. Se tomaron 10 mL de sangre a 105 novillas seleccionadas en 15 rebaños lecheros de Caldas, Colombia (5º4' N y 75º3' O) y se tomaron muestras de forrajes para analizar Cu, Fe, Mn y Zn. El promedio de la actividad de SOD fue 1.390±1.299 U g-1 Hb, y estaba correlacionada con Cu, Mn y Fe en el forraje. La actividad promedio de GSH-Px fue 389±184 U g-1 Hb y fue observada una mayor frecuencia de valores deficitarios y bajo/marginales de 9%, habiendo sido más afectados los animales de zonas altas (>2.000 msnm).... Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bovinos; Oligoelementos; Antioxidantes; Enzimas; Bovine; Oligoelements; Antioxidants; Enzymes. Ano: 2005 URL: http://www.alice.cnptia.embrapa.br/handle/doc/116902 Activity of cellulase from Thermoactinomycetes and Bacillus spp. isolated from Brassica waste compost Provedor de dados: 63 Autores: Chang,Chen-Chin; Ng,Chang-Chai; Wang,Chung-Yi; Shyu,Yuan-Tay. Plant wastes present a high cellulose content, which is an ideal organic material for composting. Five strains of thermophiles from processed Brassica waste were isolated, and the hydrolytic activity on various cellulosic biomass substrata and their temperature profiles were determined. 16S rRNA sequencing identified these strains as Thermoactinomyces and Bacillus spp. Maximal cellulase activity corresponded to 2.3 U mL-1 of enzyme. The application of these strains on Brassica rapa residues demonstrates increased total nitrogen content). TA-3, a Thermoactinomycetes sp. strain, performs best among all inoculants, increasing the nitrogen content from 0.74 to 0.91%, and decreasing the carbon content from 15.4 to 12.2%, showing its high efficiency and... Tipo: Journal article Palavras-chave: Cellulose degrading; Enzymes; Vegetable wastes. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000300004 Alterations in muscular enzymes of horses competing long-distance endurance rides under tropical climate Provedor de dados: 45 Autores: Teixeira-Neto,A.R.; Ferraz,G. C.; Moscardini,A. R. C.; Balsamão,G. M.; Souza,J. C. F.; Queiroz-Neto,A. This study tried to monitor the alterations of muscle enzymes activity - creatinokinase (CK), lactate dehydrogenase (LDH), and aspartate aminotransferase (AST) - in a group of horses that participated of 70 and 100km distance rides in five competitions of an annual endurance championship under tropical climate. Pre ride levels (U/l) were 245.13±9.84 for CK, 496.61±14.76 for LDH, and 328.95±8.65 for AST. When compared to these levels, the results revealed a significant decrease in all enzymes activities in the first moment of the rides. Peak levels, significantly different, were reached, immediately after rides by CK (413.591±50.75); 24 hours post rides by LDH (628.61±33.30); and 48 hours after rides by AST (389.89±16.96). Monitoring of recovery period... Tipo: Journal article Palavras-chave: Horse; Endurance; Enzymes; Muscle. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000300004 &#946;-galactosidases from cowpea stems: properties and gene expression under conditions of salt stress Provedor de dados: 124 Autores: Sudério,Fabrício Bonfim; Gomes Filho,Enéas; Costa,José Hélio; Enéas Filho,Joaquim. The aim of this work was to evaluate the properties and gene expression of isoforms of &#946;-galactosidase, isolated from the stems of seedlings of the cowpea cv. Pitiuba, at different stages of development and sown in the absence and presence of NaCl. Seeds were sown onto sheets of filter paper soaked in distilled water (control) and a 100 mM NaCl solution. The plants were collected at the III (ED III) and VIII (ED VIII) stages of development. Isoforms of the enzyme &#946;-gal I, II and III presented molecular weights of 89, 146 and 124 kDa respectively, and the same pH (4.0) and optimum test temperature (55 °C) under all the conditions studied. The three isoforms were stable up to 50 °C. The thermal inactivation curves of the enzymes as a... Tipo: Journal article Palavras-chave: Vigna unguiculata; Enzymes; Purification. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000400018 Analysis of binding properties and specificity through identification of the interface forming residues (IFR) for serine proteases in silico docked to different inhibitors. Provedor de dados: 119 Autores: RIBEIRO, C.; TOGAWA, R. C.; NESHICH, I. A. P.; MAZONI, I.; MANCINI, A. L.; MINARDI, R. C. de M.; SILVEIRA, C. H. da; JARDINE, J. G.; SANTORO, M. M.; NESHICH, G. Background: Enzymes belonging to the same super family of proteins in general operate on variety of substrates and are inhibited by wide selection of inhibitors. In this work our main objective was to expand the scope of studies that consider only the catalytic and binding pocket amino acids while analyzing enzyme specificity and instead, include a wider category which we have named the Interface Forming Residues (IFR). We were motivated to identify those amino acids with decreased accessibility to solvent after docking of different types of inhibitors to sub classes of serine proteases and then create a table (matrix) of all amino acid positions at the interface as well as their respective occupancies. Our goal is to establish a platform for analysis of... Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Enzimas; Propriedades ligantes; Proteases; Binding properties; Enzymes; Interface Forming Residues. Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/handle/doc/867859 Anatomy and physiology of digestive tract of crustaceans decapods reared in aquaculture Provedor de dados: 5 Autores: Ceccaldi, H. The digestive tract of crustacea is complex. It is composed of a shor t oesophagum, a stomach with two bags, and internal wall with appendags specialized in grinding of feeds. These hard mastication pieces represent the gastric mill. Setae, filters included in the stomach structure allow the separation between food particles and liquid compounds. Those one are coming over through filters and more on to the digestive gland tubules or mid gut gland. In this organ, several types of cells play specialized functions: absorption, enzyme secretion, stocking function. Some cells of a new type, with undefined function, may be neurosecretions function, have been described recently. The end of tubules of digestive gland exhibits cells with embryonic characteristics.... Tipo: Text Palavras-chave: Decapoda; Neurosecretion; Feed; Rearing; Digestive glands; Enzymes; Stomach; Crustacean culture. Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/acte-1486.pdf Antioxidant compoundsand total antioxidant activity in fruits of acerola from cv. Flor Branca, Florida Sweet and BRS 366 Provedor de dados: 94 Autores: Souza,Kellina Oliveira de; Moura,Carlos Farley Herbster; Brito,Edy Sousa de; Miranda,Maria Raquel Alcântara de. Information on antioxidant properties at different ontological stages may help producers and food technologists to identify which cultivar and/or maturity stage are most adequate for their need, therefore this work aimed to study the changes in the antioxidant metabolism during acerola development. Fruit from cv. Flor Branca, BRS366 and Florida Sweet were harvested at different stages: immature green colored (I), physiologically mature with green color and maximum size (II), breaker (III) and full red ripe (IV). After harvest, fruits were selected, divided into four replications with 500 g each and evaluated regarding their titratable acidity, pH, soluble solids, total soluble sugar, vitamin C, polyphenol, anthocyanin, yellow flavonoid, total antioxidant... Tipo: Journal article Palavras-chave: Malpighia emarginata; Development; Vitamin C; Enzymes; Polyphenols. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452014000200004 Antioxidant system response induced by aluminum in two rice cultivars Provedor de dados: 61 Autores: Ribeiro,Cleberson; Cambraia,José; Peixoto,Paulo Henrique Pereira; Fonseca Júnior,Élcio Meira da. The antioxidant defense system response was evaluated in two rice cultivars (Oryza sativa L.), Fernandes (CNA-1158) and Maravilha (CNA-6843-1), treated with toxic levels of aluminum. After exposure to aluminum we determined plant growth, H2O2 and O2&#8226;- contents, lipid peroxidation, antioxidant enzymes activities and ascorbate and dehydroascorbate contents. Al predominantly accumulated in roots of both cultivars but it reduced root and shoot growth only in the Maravilha cultivar. Treatment with aluminum resulted in a reduction of 84 and 60% in the levels of H2O2 in the roots of the cultivars Maravilha and Fernandes, respectively, and of 26% in the levels of O2&#8226;- only in the roots of Fernandes cultivar. Increased lipid peroxidation was... Tipo: Journal article Palavras-chave: Aluminum; Enzymes; Reactive oxygen species; Oryza sativa; Oxidative stress. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202012000200004 Aplicação e pontos críticos no uso de enzimas para monogástricos. Provedor de dados: 14 Autores: KRABBE, E. L. 2013 Tipo: Artigo de divulgação na mídia (INFOTECA-E) Palavras-chave: Nutrição animal; Enzima; Dieta; Animal nutrition; Enzymes; Diet. Ano: 2013 Assessing joint research impacts Provedor de dados: 30 Autores: M.C.S. Palavras-chave: Pigeonpeas; Chickpeas; Millets; Sorghum; Hybrids; Germplasm; Genetic soil types; Enzymes; Groundnuts; Double cropping. Ano: 1998 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4201 Associação de carboidrase e fitase em dietas valorizadas para poedeiras semipesadas - doi: 10.4025/actascianimsci.v34i3.12735 Provedor de dados: 2 Autores: Silva, Leandro Moreira; Instituto Federal Minas Gerais - Campus Bambuí; Geraldo, Adriano; Instituto Federal Minas Gerais - Campus Bambuí; Vieira Filho, Javer Alves; Instituto Federal Minas Gerais - Campus Bambuí; Machado, Luiz Carlos; Instituto Federal Minas Gerais - Campus Bambuí; Brito, Jerônimo Ávito Gonçalves de; Universidade Federal do Recôncavo Baiano; Bertechini, Antonio Gilberto; Universidade Fderal de Lavras. O objetivo do estudo foi avaliar o efeito da associação de carboidrases e fitase em dietas de poedeiras. Foram utilizadas 300 poedeiras Isa Brown com 26 semanas, submetidas aos tratamentos: Controle Positivo: sem enzimas e valorização dos nutrientes; T2: valorização em 1,5 e 6% a EMA (kcal kg-1), do milho e o farelo de soja, respectivamente, e redução nos níveis nutricionais conforme a matriz nutricional para a fitase; T3: valorização da EMA do milho e o farelo de soja (idem T2) e redução nos níveis de cálcio e fósforo conforme matriz nutricional da fitase; T4: T2 suplementado com 100 g t-1 de carboidrases e 30 g t-1 de fitase; T5: T3 suplementado com 100 g t-1 de carboidrases e 30 g t-1 de fitase. Foi utilizado delineamento inteiramente casualizado, seis... Tipo: Pesquisa de campo Palavras-chave: 5.04.03.00-1 desempenho; Qualidade de ovos; Enzimas energia metabolizável; Fósforo disponível; Valorização de dietas performance; Egg quality; Enzymes; Metabolizable energy; Disponible phosphorus; Valorization of diets. Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/12735 Atividade de enzimas oxidativas em raízes de mandioca tolerantes e susceptíveis à deterioração fisiológica pós-colheita. Provedor de dados: 119 Autores: MOTA, J. V. R.; PEREIRA, M. E. C.; VENTURINI, M. T.; OLIVEIRA, E. J. de; SILVA, M. L. C. A produção de mandioca (Manihot esculenta Crantz) na forma "in natura" é destinada basicamente para a alimentação humana pelo consumo das raízes, mas a sua comercialização é limitada por causa da deterioração pós-colheita que se manifesta dentro de 24 a 48h nas raízes causando perdas de qualidade, tornando-as impalatáveis e sem valor comercial. Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Polifenoloxidase; Mandioca; Peroxidase; Cassava; Manihot esculenta; Enzymes. Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1011168 Atividade de glicosidases em sementes de Caesalpinia pyramidalis Tul. Provedor de dados: 119 Autores: OLIVEIRA, T. dos R. de; SOUSA, E. M. de; BRITO, M. S. de; FIGUEIREDO, M. F.; SALLES, H. O.; ANDRADE, L. B. da S. 2013 Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Glicosidase; Enzimas hidrolíticas; Amilase; Celulase; Enzima; Catingueira; Caesalpinia pyramidalis; Amylases; Cellulase; Enzymes; Hydrolyases. Ano: 2013 Atividade enzimática de um latossolo vermelho de cerrado sob sistemas integrados lavoura-pastagens. Provedor de dados: 14 Autores: SILVA, L. G.; MENDES, I. de C.; ROLIM, L. F. S.; SANTOS, A. B.; REIS JÚNIOR, F. B.; VILELA, L. 0 Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E) Palavras-chave: Bioquímica do solo; Enzima; Cerrado; Microbiologia do solo; População microbiana; Biomassa; Soil; Biochemistry; Enzymes; Microbiology; Microbial flora; Biomass. URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/569446 Atributos microbiológicos do solo de áreas mineradas em recuperação: I - atividade de urease e arginase. Provedor de dados: 119 Autores: DUARTE, E. S.; TEIXEIRA, G. D.; FONSECA, L. M. F.; REIS, D. P.; OLIVEIRA, C. M. R.; MARRIEL, I. E.; FAGGI, A. M. 2014 Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Bioindicador; Recuperação; Enzima; Enzymes. Ano: 2014 Avaliação do hemograma e das funções hepática e renal de cabras leiteiras alimentadas com farelo de mamona destoxificado. Provedor de dados: 119 Autores: FERNANDES, M. F.; MEDEIROS, A. N. de; BOMFIM, M. A. D.; QUEIROGA, R. de C. R. do E.; PINHEIRO, R. R.; SANTIAGO, L. B. O objetivo deste estudo foi avaliar a eficácia in vivo do processo de destoxificação do farelo de mamona através da avaliação do hemograma e das funções hepática e renal de cabras leiteiras. Foram utilizadas 28 cabras da raça Anglo-Nubiana e Saanen, alojadas em baias individuais e distribuídas em um delineamento inteiramente casualizado com quatro níveis de substituição (0%, 33,3%, 66,6% e 100%) do farelo de soja pelo farelo de mamona destoxificado e sete repetições. O período experimental foi composto por 30 dias de adaptação à dieta e 5 dias para a coleta dos dados. O exame hematológico consistiu na determinação do volume globular, hemáceas, hemoglobina, volume globular médio, concentração hemoglobínica globular média, hemoglobina globular média,... Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Farelo de mamona; Função renal; Enzima hepática; Caprino; Nutrição animal; Alimento alternativo; Alimento para animal; Biodiesel; Co-produto; Ricinus communis; Mamona; Ricina; Resíduo; Subproduto; Ricinus; Goats; Biodiesel; Enzymes; Hematology. Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/handle/doc/870294 Beta-glucosidase from normal and brittle-stem mutant rice cultivars (Oryza sativa L.) : isolation, purification and characterization Provedor de dados: 34 Autores: Bongon, Joseph R. xiii, 88 leaves : ill. Thesis (M.S.) -- University of the Philippines at Los Baños Tipo: Thesis Palavras-chave: Rice; Enzymes; Beta-glucosidase; Varieties; Mutation. Ano: 1994 URL: http://hdl.handle.net/123456789/696 Biological activity of lectins from grain legumes and garlic against the legume pod borer, Helicoverpa armigera Provedor de dados: 30 Autores: Arora, R.,( Richa Arora ). Palavras-chave: Garlic; Pigeonpeas; Proteins; Transgenics; Transgenic plants; Chickpeas; Genes; Enzymes; Animal developmental stages; Genetic transformation. Ano: 2005 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3230 Caractérisation morphologique et fonctionnelle des hémocytes chez l'huître plate, Ostrea edulis Provedor de dados: 5 Autores: Xue, Quiggang. The mechanism that bivalve molluscs react to infectious agents are important research subjects in the fIeld of disease control studies for these animals. While the selection of disease resistant populations have made decisive progression in some species, the mechanism behinds these resistant phenomena is not yet clear so far. However, hemocytes must represent one of the key factors in the reaction process. Based on this hypothesis, the structure and function of European flat oyster, Ostrea edulis, hemocytes have been investigated in aid of different techniques in the present study. ln addition, Pacific oyster, Crassostrea gigas, hemocytes have been analysed in certain aspects so as to provide some results for comparison. European flat oyster and Pacific... Tipo: Text Palavras-chave: Bivalvia; Ostrea edulis; Crassostrea gigas; Mécanismes de défense; Hémocytes; Granulocytes; Hyalinocytes; Séparation; Anticorps monoclonaux; Enzymes; Cytométrie de flux; Bivalvia; Ostrea edulis; Crassostrea gigas; Defence mechanisms; Hemocytes; Granulocytes; Hyalinocytes; Separation; Monoclonal antibodies; Enzymes; Flow cytometry. Ano: 1998 URL: http://archimer.ifremer.fr/doc/00107/21820/19406.pdf Caracterização bioquímica de germoplasma de fruteiras. Provedor de dados: 14 Autores: MARTINS, C. da S.; POLTRONIERI, M. C.; GAIA, J. M. D.; IKETANI, H.; KAJITA, T.; YOSHIOKA, H. 1997 Tipo: Outras publicações técnicas (INFOTECA-E) Palavras-chave: Isoenzima; Enzima; Pimenta-do-reino; Urucu; Cupuaçu; Germoplasma; Isoenzymes; Enzymes; Piper nigrum; Bixa orellana; Theobroma grandiflorum; Germplasm. Ano: 1997 Caracterização das lipases obtidas por duas cepas de Aspergillus niger. Provedor de dados: 119 Autores: SOUSA, F. do C. A. de; SOUZA, E. F. de; TERZI, S. da C.; BRIGIDA, A. I. S.; GOTTSCHALK, L. M. F.; PENHA, E. das M. As lipases são enzimas que catalisam a hidrólise parcial ou total de triacilglicerois produzindo ácidos graxos livres, diacilglicerol, monoacilglicerol e glicerol. Este trabalho caracterizou a especificidade, a temperatura ótima e o pH ótimo das lipases obtidas por duas cepas de Aspergillus niger, sendo uma selvagem C e outra mutante 11T53A14. Os resultados mostram que os extratos enzimáticos apresentam especificidade diferentes, sendo a cepa selvagem mais específica para ácidos graxos de 8 carbonos e a cepa mutante inespecífica em relação ao tamanho do ácido graxo. As duas cepas apresentaram atividade em uma ampla faixa de pH, porém observou-se uma redução da atividade de mais de 50% em pH acima de 9,0. Em relação à temperatura, as lipases das duas cepas... Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Aspergillus; Lipase; Temperatura; PH; Enzima; Lipase; PH; Aspergillus; Enzymes. Ano: 2016 Caracterização de uma amilase de sementes de Libidibia ferrea (Mart. ex. Tul.). Provedor de dados: 119 Autores: LIMA, T. C.; ALVES, J. P. de A.; BRITO, M. S. de; FIGUEIREDO, M. F.; SALLES, H. O.; ANDRADE, L. B. da S. Caracterizar uma amilase de extratos aquosos de sementes de L. ferrea quanto à estabilidade térmica e massa molecular. Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Glicosidase; Pau-ferro; Libidibia ferrea; Potencial biotecnológico; Jucá; Caesalpinia ferrea; Espécie nativa; Leguminosa; Proteína; Enzima; Amido; Enzymes; Amylases; Starch; Indigenous organisms; Glycosidases; Proteinases. Ano: 2013 Cellulase and xylanase activity during the decomposition of three aquatic macrophytes in a tropical oxbow lagoon Provedor de dados: 58 Autores: Sciessere,L; Cunha-Santino,M. B; Bianchini Jr,I. Due to the connection between enzymatic activity and degradation of different fractions of organic matter, enzyme assays can be used to estimate degradation rates of particulate and dissolved organic carbon in freshwater systems. The aim of this study was to quantify and model the enzymatic degradation involving the decomposition of macrophytes, describing temporal activity of cellulases (EC 3.2.1.4 and EC 3.2.1.91) and xylanase (EC 3.2.1.8) during in situ decomposition of three aquatic macrophytes (Salvinia sp., Eichhornia azurea and Cyperus giganteus) on the surface and water-sediment interface (w-s interface) of an oxbow lagoon (Óleo lagoon) within a natural Brazilian Savanna Reserve. Overall, the enzymatic degradation of aquatic macrophytes in Óleo... Tipo: Journal article Palavras-chave: Enzymes; Litterbags; Mass decay; Particulate organic matter. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300009 Chemical products induce resistance to Xanthomonas perforans in tomato Provedor de dados: 58 Autores: Itako,Adriana Terumi; Tolentino Júnior,João Batista; Silva Júnior,Tadeu Antônio Fernandes da; Soman,José Marcelo; Maringoni,Antonio Carlos. The bacterial spot of tomato, caused by Xanthomonas spp., is a very important disease, especially in the hot and humid periods of the year. The chemical control of the disease has not been very effective for a number of reasons. This study aimed to evaluate, under greenhouse conditions, the efficacy of leaf-spraying chemicals (acibenzolar-S-methyl (ASM) (0.025 g.L&#8722;1), fluazinam (0.25 g.L&#8722;1), pyraclostrobin (0.08 g.L&#8722;1), pyraclostrobin + methiran (0.02 g.L&#8722;1 + 2.2 g.L&#8722;1), copper oxychloride (1.50 g.L&#8722;1), mancozeb + copper oxychloride (0.88 g.L&#8722;1 + 0.60 g.L&#8722;1), and oxytetracycline (0.40 g.L&#8722;1)) on control of bacterial spot. Tomatoes Santa Clara and Gisele cultivars were... Tipo: Journal article Palavras-chave: Solanum lycopersicum; Bacterial spot; Control; Enzymes. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300701 CHICKPEA BIBLIOGRAPHY Provedor de dados: 30 Autores: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT). Palavras-chave: Chickpeas; Grain legumes; Amino acids; Peas; Proteins; Enzymes; Sowing rates; Vitamins; Wheats; Biosynthesis. Ano: 1977 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3940 Chitinase activity in rice in relation to infection by Rhizoctonia solani Kuhn Provedor de dados: 34 Autores: Shrestha, Chandra Laxmi. xx, 130 l. : ill. Thesis (Ph.D.) -- University of the Philippines at Los Baños Tipo: Thesis Palavras-chave: Rice; Chitinase; Rhizoctonia solani virus; Disease resistance; Enzymes. Ano: 1996 URL: http://hdl.handle.net/123456789/600 Cold induced changes on sugar contents and respiratory enzyme activities in coffee genotypes Provedor de dados: 65 Autores: Partelli,Fábio Luiz; Vieira,Henrique Duarte; Rodrigues,Ana Paula Dias; Pais,Isabel; Campostrini,Eliemar; Chaves,Maria Manuela Coelho Cabral; Ramalho,José Cochicho. The present research aimed to characterize some biochemical responses of Coffea canephora (clones 02 and 153) and C. arabica (Catucaí IPR 102) genotypes subjected to low positive temperatures, helping to elucidate the mechanisms involved in cold tolerance. For that, one year old plants were subjected successively to 1) a temperature decrease (0.5&deg;C a day) from 25/20&deg;C to 13/8&deg;C (acclimation period), 2) a three day chilling cycle (3x13/4&deg;C) and to 3) a recovery period of 14 days (25/20&deg;C). In Catucaí (less cold sensitive when compared to clone 02) there was an increased activity in the respiratory enzymes malate dehydrogenase and pyruvate kinase. Furthermore, Catucaí showed significant increases along the cold... Tipo: Journal article Palavras-chave: Coffee tree; Coffea sp.; Sucrose; Enzymes; Cold stress. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000400006 Combined application of Pseudomonas fluorescens and Trichoderma viride has an improved biocontrol activity against stem rot in groundnut Provedor de dados: 30 Autores: Manjula, K. Palavras-chave: Biological control; Biochemical compounds; Bacteria; Poultry equipment; Fungi; Enzymes; Fungicides; Antibiotics; Seed treatment; Chitin. Ano: 2004 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3322 Comparação da produção de enzimas celulolíticas e hemicelulolíticas por linhagens mutantes e parental do Aspergillus niger 3T5B8. Provedor de dados: 119 Autores: GOTTSCHALK, L. M. F.; TERZI, S. da C.; SOUZA, E. F. de; OLIVEIRA, E. M. M.; FAVARO, L. C. de L.; PENHA, E. das M. Diversos trabalhos têm procurado aumentar a eficiência da hidrólise enzimática da biomassa lignocelulósica. Nesse contexto, o melhoramento de cepas produtoras de enzimas celulolíticas e hemicelulolíticas pode resultar em misturas enzimáticas mais eficientes. A linhagem parental de Aspergillus niger 3T5B8, referenciada como produtora de poligalacturonase, foi utilizada para o melhoramento genético visando aumentar a produção de celulases e hemicelulases. A produção das enzimas CMCase, xilanase, beta-glicosidase e poligalacturonase por fermentação submersa usando duas linhagens mutantes P49 e P83 foi avaliada e comparada com a linhagem parental. Os resultados mostraram um destaque para a cepa P83 com um aumento na produção de 56% de CMCase, 76% de... Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Celulases; Xilanases; Beta-glicosidase; Aspergillus niger; Celulase; Enzima; Aspergillus niger; Enzymes. Ano: 2016 Comparação de duas soluções extratoras na resolução de cinco sistemas enzimáticos em folhas jovens de cupuaçuzeiro (Theobroma grandiflorum Schum.). Provedor de dados: 14 Autores: COSTA, M. R.; GAIA, J. M. D.; POLTRONIERI, M. C. 1999 Tipo: Circular Técnica (INFOTECA-E) Palavras-chave: Cupuaçu; Folha; Extração de enzima; Eletroforese de isoenzima; Substância extratora; Theobroma grandiflorum; Leaves; Enzymes; Electrophoresis; Isoenzymes; Analytical methods. Ano: 1999 Compostos antioxidantes e atividade antioxidante total em frutos das variedades Flor Branca, Florida Sweet e BRS 366 de aceroleira. Provedor de dados: 119 Autores: SOUZA, K. O.; MOURA, C. F. H.; BRITO, E. S. de; MIRANDA, M. R. A. de. 2014 Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Malpighia emarginata; Desenvolvimento; Vitamina C; Enzimas; Polifenóis; Malpighia emarginata; Development; Vitamin C; Enzymes; Polyphenols. Ano: 2014 Definição de protocolos de eletroforese para análise de isoenzimas em pimenta-do-reino (piper nigrum L.). Provedor de dados: 14 Autores: OLIVEIRA, M. R. C. de; POLTRONIERI, M. C.; MARTINS, C. da S.; GAIA, J. M. D. 1998 Tipo: Séries anteriores (INFOTECA-E) Palavras-chave: Pimenta-do-reino; Isoenzima; Extração de enzima; Macador genético; Eletroferese; Gel de amido; Gel poliacriamida; Piper nigrum; Pará; Amazônia; Brasil; Isoenzymes; Enzymes; Genetic markers; Electropharesis. Ano: 1998 Desempenho e atividade de amilase em tilápias-do-nilo submetidas a diferentes temperaturas Provedor de dados: 119 Autores: MOURA, G. de S.; OLIVEIRA, M.G.A.; LANNA, E.T.A.; JÚNIOR, A.M.; MACIEL, C.M.R.R. O objetivo deste trabalho foi avaliar o desempenho e a atividade de amilase em quimo de tilápias-do-nilo macho, linhagem tailandesa, submetidas a quatro diferentes temperaturas. O experimento foi conduzido em delineamento inteiramente casualizado, com quatro tratamentos (20, 24, 28 e 32oC), seis repetições e dez peixes por unidade experimental. A dieta utilizada foi igual para todos os tratamentos. Aos 55 dias do experimento, o consumo de ração aparente, ganho de peso, conversão alimentar aparente, atividade de amilase e atividade específica da amilase foram avaliados. O consumo de ração aparente e o ganho de peso aumentaram linearmente com o aumento da temperatura. Na conversão alimentar aparente, foi observado efeito quadrático em função da temperatura... Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Oreochromis niloticus; Enzimas; Fisiologia de peixes; Piscicultura; Enzymes; Fish physiology; Pisciculture. Ano: 2007 URL: http://www.alice.cnptia.embrapa.br/handle/doc/122831 Detecção da presença da enzima beta-galactosidase em bactérias láticas. Provedor de dados: 119 Autores: MATOS, C. R. de; SANTOS, K. M. O. dos. 2013 Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Â-galactosidase; Bactéria láctica; Intolerância à lactose; Lactose intolerance; Food intolerance; Caprino; Leite de cabra; Lactose; Enzima; Alpha galactosidase; Goat milk; Enzymes; Food allergies. Ano: 2013 Detection and analysis of C1p protease mRNA in rice using RT-PCR and northern hybridization Provedor de dados: 34 Autores: Chaudhary, Priyanka. xii, 76 l. : ill. Thesis (M.S.) -- University of the Philippines at Los Baños Tipo: Thesis Palavras-chave: Proteinases; Reverse transcription polymerase chain reaction; Rice; Plant genetics; Messenger RNA; Leaves; Enzymes. Ano: 1996 URL: http://hdl.handle.net/123456789/622 Development of cost-effective SNP assays for chickpea genome analysis and breeding Provedor de dados: 30 Autores: Varshney, R.K.,( Rajeev K. Varshney ). Palavras-chave: Genomes; Polymorphism; Genotypes; Enzymes; Nucleotides; Chickpeas; DNA; Bioinformatics; Genetic structures; Genes. Ano: 2007 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3282 Distribuicao da atividade enzimatica no perfil de um latossolo vermelho amarelo de cerrado sob vegetacao nativa e sob sistemas de plantio direto e convencional. Provedor de dados: 14 Autores: MENDES, I. de C.; REIS JUNIOR, F. B. dos; P. NETO, J. V. 0 Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E) Palavras-chave: ECOSOL160; Bioquimica do solo; Cerrado; Enzima; Perfil do solo; Plantio direto; Soil; Biochemistry; Enzymes; Soil profiles; Zero tillage. URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/567257 Diversidad bacteriana en el tracto digestivo de la lombriz de tierra Pontoscolex corethrurus. Provedor de dados: 32 Autores: Brito Vega, Hortensia. El objetivo del presente trabajo fue estudiar la diversidad bacteriana en el contenido digestivo de la lombriz de tierra endogea Pontoscolex corethrurus. El muestreo de la especie P. corethrurus se realizó en dos localidades de Veracruz, México: Plan de las Hayas, zona ganadera con suelo arcilloso y La Mancha, zona de reserva ecológica y con suelo arenoso. El intestino de las lombrices fue dividido en cuatro secciones (A, B, C y D). Bajo condiciones asépticas, se prepararon diluciones decimales con el contenido intestinal empleando el medio líquido Brain Heart Infusion. Posteriormente, la suspensión del contenido intestinal se sembró en medios específicos. La identificación taxonómica de los aislamientos bacterianos puros se realizó por dos... Palavras-chave: Anisosimbiosis; Lombriz de tierra; Enzimas; Biodiversidad; Earthworm; Enzymes; Biodiversity; Doctorado; Edafología. Ano: 2010 URL: http://hdl.handle.net/10521/265 Dynamique et enzymologie de la digestion chez l'huitre Crassostrea gigas Provedor de dados: 5 Autores: Boucaud, C; Lebesnerais, C; Lubet, P; Lihrmann, I. Feeding experiments carried out on Crassostrea gigas allowed the authors to set up the stages and the timing of the movement of food throughout the digestive tract. Various activities involved in digestion (glycosidases, esterases, and peptidases) were investigated histochemically in the digestive tract in order to localize the sites of absorption and the sites of protein and carbohydrate digestion. An explicative model of the different stages of digestion is proposed. Tipo: Text Palavras-chave: Crassostrea gigas; Bivalvia; Cytology; Histochemistry; Histology; Digestive system; Enzymatic activity; Digestive glands; Digestion; Enzymes; Transit digestif; Digestion; Huitre; Crassostrea gigas. Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1179.pdf Early weaning of marine fish larvae onto microdiets: Constraints and perspectives Provedor de dados: 5 Autores: Person, L. Weaning small sized marine fish larvae at first feeding directly onto compound pellets is still difficult, while good results can be obtained when used in combination with live prey or when a prefeeding period on live prey is provided up to a size of about 2-3 mg. Microdiets are generally well ingested as their acceptability and visual detection may be improved using feeding activators and increasing good perception contrast. In theory, food may be processed to have a correct nutritional balance, selection of high digestible components is very important as larval digestive system is not fully functional during the first 2 or 3 weeks of life. Tipo: Text Palavras-chave: Hatching; Fatty acids; Amino acids; Nutritional requirements; Feeding behaviour; Stomach; Digestive system; Enzymes; Digestion; Physiology; Diets; Pellet feeds; Feeding; Fish larvae. Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/acte-1422.pdf Efeito da insulação nos níveis enzimáticos de AST e ALT no plasma seminal de caprinos. Provedor de dados: 119 Autores: PINHEIRO, R. R.; SALLES, H. O; AZEVEDO, H. C.; SIMPLICIO, A. A.; SANTOS, D. O.; ANDRIOLI, A. 1993 Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Caprino; Sêmen; Bioquímica; Enzima; Goats; Enzymes; Biochemistry. Ano: 1993 Efeito do uso de fitase em dietas de frangos de corte sobre a biodisponibilidade de minerais e aproveitamento de energia. Provedor de dados: 119 Autores: AVILA, V. S. de; KRABBE, E. L.; KLEIN, C. H.; LOPES, L. dos S.; SANDRI, E. C.; MAIORKA, A. 2013 Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Nutrição animal; Dieta; Ração; Frango de corte; Animal nutrition; Broiler feeding; Enzymes; Phytases. Ano: 2013 Effect of Fenoxaprop on acetyl-CoA carboxylase (ACCase) activity in rice (Oryza sativa L.), Echinochloa crus-galli (L.) Beauv. and Echinochloa glabrescens Munro ex Hook f. Provedor de dados: 34 Autores: Completo, Gladys Cherisse J. ix, 52 leaves : ill. Thesis (M.S.) -- University of the Philippines at Los Baños Tipo: Thesis Palavras-chave: Rice; Enzymes; Herbicides; Fenoxaprop; Acetyl-CoA carboxylase; Echinochloa crus-galli. Ano: 1998 URL: http://hdl.handle.net/123456789/555 Effect of protease supplementation on production performance of laying hens Provedor de dados: 2 Autores: Vieira Filho, Javer Alves; Geraldo, Adriano; Machado, Luiz Carlos; Brito, Jerônimo Ávito Golçalvez de; Bertechini, Antônio Gilberto; Murakami, Elise Saori Floriano. The experiment was conducted with the objective of evaluating the effect of protease enzyme supplementation on performance parameters and quality shell of laying hens. We used 240 Isa Brown commercial laying hens with 44 weeks of age. A completely randomized split-plot design (5 periods of 21 days) with four treatments and six replications (10 hens per replication) was used. The experimental diets were formulated according to the requirements of the breed. The following parameters were evaluated: egg production, feed intake, feed conversion, average egg weight, egg loss, specific gravity, percentage and shell thickness. After collection, the data were analyzed with SISVAR Statistical Package, and the means compared by SNK test at 5% probability. It was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Laying hen farming; Enzymes; Grains; Nutrition. Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/22830 Effects of commercial enzymes on the adhesion of a marine biofilm-forming bacterium Provedor de dados: 5 Autores: Leroy, C.; Delbarre Ladrat, Christine; Ghillebaert, F.; Compere, Chantal; Combes, D. The antifouling potential of commercial hydrolases, four proteases, seven glycosidases and one lipase was evaluated on the adhesion of marine Pseudoalteromonas sp. D41. The experimental method, adapted to screen antifouling agents, was based on bacterial adhesion in natural sterile sea water in a microtiter plate and on total biomass quantification by the fluorescent dye DAPI (4[prime]6-diamidino-2-phenylindole). Savinase (subtilisin) was the most effective hydrolase in both the prevention of bacterial adhesion and the removal of adhered bacteria. However, some enzymatic preparations tested such as Amano protease were not only ineffective but also increased the number of adhered bacterial cells. Enumeration using epifluorescence microscopy of CTC... Tipo: Text Palavras-chave: Marine biofilm; Antifouling; Pseudoalteromonas; Enzymes; Adhesion. Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6140.pdf Effects of cover crops species on Passiflora edulis nutrition. Provedor de dados: 14 Autores: ANDRADE, L. R. M. de; CARVALHO, A. M. de; MENDES, I. de C.; VIVALDI, L.; KARIA, C. T.; JUNQUEIRA, N. T. V. 0 Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E) Palavras-chave: Planta de cobertura; Solo; Enzima; Produtividade; Nutricao vegetal; Maracuja; Cover plants; Soil; Enzymes; Plant nutrition; Crop yield. URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/567640 Effects of enzyme complex SSF (solid state fermentation) in pellet diets for Nile tilapia Provedor de dados: 96 Autores: Moura,Guilherme de Souza; Lanna,Eduardo Arruda Teixeira; Filer,Keith; Falkoski,Daniel Luciano; Donzele,Juarez Lopes; Oliveira,Maria Goreti de Almeida; Rezende,Sebastião Tavares de. The effects of enzyme complex SSF (solid state fermentation) on growth performance and the availability of sucrose and monosaccharides in the chyme of Nile were involved. The study included 360 fish (70g±4.43) in a completely randomized design with six dietary treatments (0, 50, 100, 150, 200 and 250 ppm of SSF) arranged in six replicates, with 10 fish per replicate. Every 15 days, one tilapia of each experimental unit was sacrificed for analyses of carbohydrate in the chyme. On day 60 of the experiment, the performance parameters were measured. There was a linear effect according to treatment for final weight and weight gain. For the other performance parameters, there were no differences. There was quadratic effect for sucrose and glucose in function of... Tipo: Journal article Palavras-chave: Aquaculture; Enzymes; Fish nutrition; HPLC; Oreochromis niloticus. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000001 Effects of hydric deficiency on gas exchange parameters and metabolism of Eucalyptus grandis clones Provedor de dados: 61 Autores: Coscolin,Renata B. S.; Broetto,Fernando; Marchese,José A.; Campohermoso,Marco C.; Paladini,Marcos V. The metabolic effects caused by hydric deficiency (HD) on Eucalyptus grandis clones were assessed by an experiment where plants were cultivated in four blocks. The first was the control block, normally irrigated, whereas the other three blocks were submitted to cycles of hydric deficiency. Analysis of photosynthetic efficiency, enzymatic activity of antioxidant response system, level of pigments and L-proline concentration were performed to evaluate the HD effects. Results showed that HD altered some parameters related to photosynthetic activity, pigments accumulation, proline and enzymatic activity. Clone 433 of E. grandis presented higher response ability to HD. Tipo: Journal article Palavras-chave: Enzymes; Eucalypt; Hydric deficiency; Photosynthesis. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000400002 Effects of lines and inoculants on nutritive value and production costs of triticale silages Provedor de dados: 96 Autores: Sucu,Ekin; Çifci,Esra Aydo&#287;an. ABSTRACT The current study was undertaken to investigate the ensilage characteristics in triticale lines treated by inoculants and their interaction on fermentation metabolites and rumen degradability. Costs were estimated for growing and feeding whole-crop triticale lines for animal production. Triticale hybrids were harvested at the dough stage of maturity (38% dry matter, DM). Plants were chopped approximately 2 cm after harvest and then treated with inoculants and were ensiled in 1.5-L mini laboratory silos. Two lactic acid bacterial inoculants with enzymes (LAB+enzymes I: Pediococcus acidilactici, Lactobacillus plantarum, and Streptococcus faecium with cellulase, hemicellulase, pentosanase, and amylase; LAB+enzyme II: P. acidilactici, L. plantarum,... Tipo: Journal article Palavras-chave: Cost; Degradability; Enzymes; Fermentation; Lactic acid bacteria; Triticale lines. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016000700355 Effet de l'incorporation de farines de soja et de poisson dans l'aliment sur la croissance et les enzymes digestives de Penaeus vannamei Provedor de dados: 5 Autores: Galgani, Francois; Ceccaldi, Hubert J.; Aquacop, Aquacop. In order to improve the formulation of diets fed to reared Penaeus vannamei, the effects of soya bean and fish meals on the growth, survival, and the characteristics of the hepatopancreas and digestive enzymes of the species were studied. Four soya bean meal concentrations (0, 5, 15, 40%), four fish meal concentrations (0, 5, 15, 30%), two forms of soya bean meal and three fish meals of different geographical origin were tested. Experimental conditions were as follows: ten individuals were placed in each tank (0.5 m2 ) and two tanks were subjected to each treatment. Food was given twice a day (08-09.00 h and 19.00 h) at 10% of the initial weight each day. For biochemical analyses, nine individuals per treatment were used for the determination of the... Tipo: Text Palavras-chave: Aquaculture; Nutrition; Pénéidés; Soja; Farine de poisson; Enzymes; Enzymes digestives.; Aquaculture; Nutrition; Penaeidae; Soya bean; Fish meal; Digestive enzymes. Ano: 1988 URL: http://archimer.ifremer.fr/doc/00135/24597/22626.pdf Effet des hautes pressions sur les indicateurs de maturation de la viande et d'altération du muscle de poisson Provedor de dados: 5 Autores: Cheret, Romuald. This study deals with the influence of the high pressure process on the ageing of meat and the degradation of the fish muscle. The aim is to characterize the effect of the high pressure treatment on the protease activities responsible for degradation for the sea bass muscle (Dicentrarchus labrax L.) and bovine muscle (Biceps femoris), but also on the texture, the colour and the microstructure. The samples were pressurized between 0 and 500 MPa during 5 minutes, and were stored at 4°C. The different proteasic systems involved in the post-mortem degradation of the muscle (mainly cathepsins and calpains) were quantified after the high pressure treatment and during the post-mortem storage. The results shown that the sensitivity of the proteases depended on... Tipo: Text Palavras-chave: Enzymes; Muscular proteins; Bovine muscle; Fish muscle; Proteases; High pressure; Protéines musculaires; Enzymes; Viande bovine; Muscle de poisson; Protéases; Hautes pressions. Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-553.pdf Eficiencia de uso de nitrogênio em sorgo: produtividade de grãos e atividade de enzimas de assimilação de carbono e nitrogênio. Provedor de dados: 119 Autores: PURCINO, A. A. C.; ANDRADE, S.R.M.; SANTOS, F.G.; OLIVEIRA, A. C.; SILVA, M.R. 1995 Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Microbiologia; Nitrogenio; Enzimas; Carbono; Nitrogenase; Microbiology; Nitrogen; Enzymes; Carbon. Ano: 1995 Electrochemical biosensors in pharmaceutical analysis Provedor de dados: 60 Autores: Gil,Eric de Souza; Melo,Giselle Rodrigues de. Given the increasing demand for practical and low-cost analytical techniques, biosensors have attracted attention for use in the quality analysis of drugs, medicines, and other analytes of interest in the pharmaceutical area. Biosensors allow quantification not only of the active component in pharmaceutical formulations, but also the analysis of degradation products and metabolites in biological fluids. Thus, this article presents a brief review of biosensor use in pharmaceutical analysis, focusing on enzymatic electrochemical sensors. Tipo: Journal article Palavras-chave: Biosensors; Pharmaceutical analysis; Electrochemical sensors; Enzymes. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300002 Electrophoretic protein pattern and acid phosphatase activity in the midgut extracts of Apis mellifera L. (Hymenoptera: Apidae) during metamorphosis Provedor de dados: 82 Autores: Cavalcante,Vagner M.; Cruz-Landim,Carminda da. The electrophoretic pattern of proteins and the activity of acid phosphatase were described in the midgut extracts during the post-embryonic stages of Apis mellifera L. in order to stablish a correlation with the metamorphosis events. The results show the greatest variation in proteic electrophoretical bands during prepupal stage and the smallest variation during the brown eyed pupae stage. The acid phosphatase activity was higher during the larval last instar and lower in white eyed pupae. The greatest variety of bands during the prepupal stage coincides with the histolysis of the larval midgut epithelium and the lowest variability in the brown eyed pupa coincides with the end of the midgut differentiation. In another hand, the greatest phosphatase... Tipo: Journal article Palavras-chave: Differentiation; Enzymes; Histolysis; Larva; Prepupa; Post-embryonic development; Pupa. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2004000200007 Environmental and physiological factors that affect the efficacy of herbicides that inhibit the enzyme protoporphyrinogen oxidase: a literature review Provedor de dados: 87 Autores: Matzenbacher,F.O.; Vidal,R.A.; Merotto Jr.,A; Trezzi,M.M. Herbicides that inhibit the enzyme protoporphyrinogen oxidase (PROTOX) are usually effective to control dicotyledonous weeds and their agronomic efficacy is affected by environmental and physiological factors. The objective of this review is to summarize the knowledge of those factors available in the scientific literature in the last decade. Environmental factors that influence PROTOX inhibitors include temperature, irradiance and relative humidity. The most relevant physiological factors are the activity of enzymes that can detoxify herbicides and also of enzymes that mitigate the effects of oxidative stress in plants. The study also suggests some possible management strategies that could optimize the activity of PROTOX-inhibiting herbicides. Tipo: Journal article Palavras-chave: Temperature; Light; Relative humidity; Enzymes. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582014000200024 Enzimas em líquidos iônicos: Uma combinação promissora para um biodiesel limpo. Provedor de dados: 119 Autores: MEDEIROS, G. A.; GONCALVES, S. B.; RODRIGUES, D. de S.; NETO, B. A. D. 2013 Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Biodiesel; Enzymes; Ionic liquids. Ano: 2013 Enzymatic Activity in the Gastrointestinal Tract of Pimelodus maculatus (Teleostei, Siluriformes) in Two Neotropical Reservoirs with Different Trophic Conditions Provedor de dados: 52 Autores: Duarte,Silvana; Bemquerer,Marcelo; Araújo,Francisco Gerson. Enzymatic activities for digestion of proteins and carbohydrates were compared among three organs of the digestive system of Pimelodus maculatus in two reservoirs with different trophic conditions during the winter of 2006. The aim was to test the hypothesis that enzymatic activity for the digestion of proteins and carbohydrates differed among organs and that such activities differ between the trophic state of the environment. Enzymatic activities were determined through the assays of specificity for trypsin, chymotrypsin and &#946;-glucosidase enzymes. The intestine had higher trypsin-like enzymatic activities compared to the stomach and liver. The highest &#946;-glucosidase activity was found in the liver compared to the stomach and intestine in... Tipo: Journal article Palavras-chave: Enzymes; Catfish; Physiology; Freshwater fishes; Digestive tract. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400605 Enzymatic activity of endophytic bacterial isolates of Jacaranda decurrens Cham. (Carobinha-do-campo) Provedor de dados: 52 Autores: Carrim,Aysha Jussara Ivonilde; Barbosa,Edweis Cândida; Vieira,José Daniel Gonçalves. The objectives of this work was to isolate endophytic bacteria from Jacaranda decurrens Cham. and screening of some enzymes of biotechnological interest. Ten (10) bacterial species were isolated and identified from the leaves and steams. All the isolates presented enzymatic activity, which was ranked as follows: proteolytic (60%) and amilolytic activity (60%), lipolytic (40%), esterasic (40%). However, cellulolytic and pectinolytic activities were not detected. This is the first report on the isolation and identification of endophytic bacteria from Jacaranda decurrens Cham. Tipo: Journal article Palavras-chave: Endophytic bacteria; Jacaranda decurrens; Enzymes. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400001 Enzymatic and immunological properties of Bungarus flaviceps (red-headed krait) venom Provedor de dados: 80 Autores: Tan,NH; Fung,SY; Ponnudurai,G. Bungarus flaviceps (red-headed krait) venom presents an intravenous LD50 of 0.32 &#956;g/g and exhibits enzymatic activities similar to other Bungarus toxins. ELISA cross-reactions between anti-Bungarus flaviceps and a variety of elapid and viperid venoms were observed in the current study. Double-sandwich ELISA was highly specific, since anti-B. flaviceps serum did not cross-react with any tested venom, indicating that this assay can be used for species diagnosis in B. flaviceps bites. In the indirect ELISA, anti-B. flaviceps serum cross-reacted moderately with three different Bungarus venoms (9-18%) and Notechis scutatus venom, but minimally with other elapid and viperid toxins. The results indicated that B. flaviceps venom shares common epitopes... Tipo: Journal article Palavras-chave: Bungarus flaviceps venom; Enzymes; ELISA; Neutralization. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100014 Enzymatic and toxinological activities of Hypnale hypnale (hump-nosed pit viper) venom and its fractionation by ion exchange high performance liquid chromatography Provedor de dados: 80 Autores: Tan,CH; Sim,SM; Gnanathasan,CA; Fung,SY; Ponnudurai,G; Pailoor,J; Tan,NH. Hypnale hypnale (hump-nosed pit viper) has been recently identified as one of the medically important venomous snakes in Sri Lanka and on the southwestern coast of India. The characterization of its venom is essential for understanding the pathophysiology of envenomation and for optimizing its management. In the present study, the biological properties of Hypnale hypnale venom and venom fractions obtained using Resource Q ion exchange chromatography were determined. The venom exhibited toxic activities typical of pit viper venom, comparable to that of its sister taxon, the Malayan pit viper (Calloselasma rhodostoma). Particularly noteworthy were its high activities of thrombin-like enzyme, proteases, phospholipase A2, L-amino acid oxidase and... Tipo: Journal article Palavras-chave: Hypnale hypnale; Venom; Enzymes; Toxins; Fibrinogen; Nephrotoxicity. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400015 Enzymatic programs for broilers Provedor de dados: 52 Autores: Sorbara,José Otávio Berti; Murakami,Alice Eiko; Nakage,Eliane Saiuri; Piracés,Francisco; Potença,Alexandra; Guerra,Rafael Lachinski Holanda. Two trials were conducted on floor pens to evaluate the performance of broilers fed with diets contained different carbohydrases enzyme programs at different ages. Trial 1- The body weight gain (BWG) was affected by the Negative Control (NC) treatment (P<0.05). However, when the birds were fed with the NC diet + Amilase (AMY)+Xilanase (XYL), the BWG improved and reached the same level as the Positive Control (PC). Trial 2- The birds received enzymes supplementation in the total phase and others only in the grower phase.The ME reduction by 120 kcal/kg increased the feed conversion ratio (FCR) (P<0.05) when compared to PC and none of the enzyme programs was able to recover the performance. It can be concluded that it is possible to supplement with AMY... Tipo: Journal article Palavras-chave: Broilers; Carbohydrase; Enzymes; Amylase; Xylanase. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700030 Enzyme activity in soybean seeds produced under foliar application of manganese Provedor de dados: 64 Autores: Carvalho,Everson Reis; Oliveira,João Almir; Von Pinho,Édila Vilela de Resende; Costa Neto,Jaime. Several factors affect the quality of soybean seeds, including the mineral nutrition of plants. Manganese (Mn) is an important nutrient because it has as one of its functions the enzyme activation. The aim of this study was to evaluate enzyme activity in seeds of soybean cultivars produced with foliar application of different doses of Mn. The experiment was conducted at the Universidade Federal de Lavras (UFLA) (Federal University of Lavras) in randomized blocks with three replications and 4 x 4 x 2 factorial arrangement consisting of four soybean cultivars, two conventional and its genetically modified RR derived (BRS Celeste and BRS Baliza RR; BRSGO Jataí and BRS Silvânia RR), four doses of Mn via foliar application (0; 200; 400 and 600 g Mn ha-1) and... Tipo: Journal article Palavras-chave: Enzymes; Glycine max; Quality; Micronutrients. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542014000400001 Enzyme polymorphism between Oryza sativa and O. minuta and its use to monitor introgression in O. sativa/O. minuta backcross derivatives Provedor de dados: 34 Autores: Romero, Gabriel Ortega. 87 l. : ill. Thesis (M.S.) -- University of the Philippines at Los Baños Tipo: Thesis Palavras-chave: Rice; Plant genetics; Enzymes; Polymorphism; Oryza minuta; Backcrossing; Introgression. Ano: 1989 URL: http://hdl.handle.net/123456789/1121 Enzyme polymorphism in rice : relation to pollen fertility and blast resistance Provedor de dados: 34 Autores: Dekuku, Roger Chris. 162 l. : ill. Thesis (Ph.D.) -- University of the Philippines at Los Baños Tipo: Thesis Palavras-chave: Rice; Enzymes; Polymorphism; Plant fertility; Blast disease; Disease resistance; Plant genetics. Ano: 1991 URL: http://hdl.handle.net/123456789/953 Enzymes of Significance to the Restoration of Polluted Systems: Traditional and Advanced Approaches Provedor de dados: 127 Autores: Gianfreda,L. Tipo: Journal article Palavras-chave: Xenobiotics; Enzymes; Soil bioremediation. Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912008000400004 Evaluating the effects of formulated moist diets on juveniles of Patagonian Octopus Enteroctopus megalocyathus (Gould 1852) Provedor de dados: 5 Autores: Farias, Ana; Pereda, Sandra V.; Uriarte, Iker; Doerner, Jessica; Cuzon, Gerard; Rosas, Carlos. The aim of this study was to evaluate the performance of Patagonian octopus fed with moist diets formulated with several local feed ingredients. All formulated diets were based on crab paste (70%) and the experimental feed ingredient (30%). Experiment I assayed salmon meal, prime sardine meal, and wheat gluten, using fresh fish as a control; experiment 2 assayed prime fish meal and macroalgal meal against crab paste alone as a control. The ingestion rate was lower than expected for all diets except those of fresh fish, crab paste alone, and crab paste plus prime sardine meal. No significant differences were found in the observed digestibility of the diets, indicating, in general, low digestibility, even for fresh fish. The highest protease values were... Tipo: Text Palavras-chave: Octopus; Emeroctopus megalocyathus; Digestibility; Enzymes; Consumption rate; Nutrition; Moist diets. Ano: 2010 URL: http://archimer.ifremer.fr/doc/00030/14106/11342.pdf Evaluation of agroindustrial residues as substrates for cellulolytic enzymes production under solid state fermentation. Provedor de dados: 119 Autores: RODRIGUEZ-ZUNIGA, U. F.; LEMO, V.; FARINAS, C. S.; BERTUCCI NETO, V.; COURI, S. 2008 Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Enzymes. Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/26632 Evaluation of nutrient excretion and retention in broilers submitted to different nutritional strategies. Provedor de dados: 119 Autores: GRAÑA, A. L.; TAVERNARI, F. de C.; LELIS, G. R.; ALBINO, L. F. T.; ROSTAGNO, H. S.; GOMES, P. C. 2013 Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Nutrição animal; Frango de corte; Enzima; Fósforo; Enzima; Animal nutrition; Broiler chickens; Enzymes; Phosphorus. Ano: 2013 Evaluation of the protective effect of guava fruits and leaves on oxidative stress 10.4025/actascibiolsci.v36i1.19839 - doi: Provedor de dados: 3 Autores: Freire, Juliana Mesquita; Faculdades Unidas do Vale do Araguaia; Abreu, Celeste Maria Patto de; Universidade Federal de Lavras; Duarte, Stella Maris da Silveira; Universidade Federal de Alfenas; Paula, Fernanda Borges Araújo de; Universidade Federal de Alfenas; Lima, Adriene Ribeiro; Universidade Federal de Lavras. Oxidative stress is an imbalance between reactive oxygen species and antioxidant capacity of action. Thus, research, alternative to mitigate the damaging effects of oxidative stress, improving the body´s antioxidant capacity, prevented the disease and its complicatons. The leaves and fruits of guava are rich in the antioxidants. This work aimed to study the effect of flour and ethanolic/acetone extracts leaves and fruits of guava on lipid oxidation in rats with hypercholesterolemic. The flour and extracts decreased leves of triglycerides and non – HDL cholesterol and increased in the HDL. Cholesterol levels decreased only leaves and fruits. The activity of GPx decreased in samples serum in all treatments and the SOD only the extracts were effective. There... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioquímica antioxidants; Enzymes; Malonic dialdehyde Metabolismo e bioenergéetica. Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/19839 Extraction de lipides en voie aqueuse par bioréacteur enzymatique combine à l'ultrafiltration : application a la valorisation de co-produits de poisson (Sardina pilchardus) Provedor de dados: 5 Autores: Dumay, Justine. The main goal of this work was to up-grade sardine (Sardina pilchardus) by-products using mild procedure, environmentally sound, in order to obtain valuable compounds involving moderate investment and low energy consumption. Hydrolysis and ultrafiltration techniques have been investigated. Firstly, selected enzymes have been calibrated in order to determine their optimal conditions with a model substrate and to permit the comparison between them. Then, hydrolysis on sardine head and viscera have been carried out in two steps. The first one had the objective to determine the enzyme efficiency and the study area. The second step was to optimise enzymatic hydrolysis using experimental designs. The aim of this second step was to obtain the highest lipid... Tipo: Text Palavras-chave: Up grading; By products; Ultrafiltration; Enzymes; Lipids; Sardina pilchardus; Valorisation; Co produits; Ultrafiltration; Enzymes; Lipides; Sardina pilchardus. Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/these-1556.pdf Feed efficiency in Mangalarga Marchador foals fed diet supplemented with probiotics or phytase Provedor de dados: 96 Autores: Moura,Raquel Silva de; Saliba,Eloísa de Oliveira Simões; Almeida,Fernando Queiroz de; Lana,Ângela Maria Quintão; Silva,Vinícius Pimentel; Rezende,Adalgiza Souza Carneiro de. The objective was to assess the effect of probiotic or phytase supplementation on dry matter intake (DMI), average daily gain (ADG) and feed conversion (FC) in 24 Mangalarga Marchador foals (16 males, 8 females), with 241.6 ± 23.2 days of age, and 216.7 ± 27.8 kg of body weight (BW). The study lasted 28 days, the first 23 days for diet and handling adaptation, and the last five days for feces collection. The basal diet consisted of Cynodon pasture and pelleted concentrate (1.2 kg/100 kg BW), as well as water and mineral salt ad libitum. Treatments groups were assigned as follows: basal diet (control); basal diet + 5 g/day of live yeast culture (10(9) CFU/g of S. cerevisiae); basal diet + 2 g/day of live bacteria (10(8) CFU/g of B. bifidum, E. faecium, L.... Tipo: Journal article Palavras-chave: Enzymes; Horses; Lactobacillus; LIPE®; Pasture intake; Saccharomyces cerevisiae. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009000600011 Fusarium graminearum growth inhibition mechanism using phenolic compounds from Spirulina sp Provedor de dados: 116 Autores: Pagnussatt,Fernanda Arnhold; Kupski,Larine; Darley,Francine Toralles; Filoda,Paula Freitas; Ponte,Émerson Medeiros Del; Garda-Buffon,Jaqueline; Badiale-Furlong,Eliana. The application of natural antifungal substances is motivated by the need for alternatives to existing methods that are not always applicable, efficient, or that do not pose risk to consumers or the environment. Furthermore, studies on the behaviour of toxigenic species in the presence of natural fungicides have enabled their safe application in the food chain In this study, Spirulina LEB-18 phenolic extract was assessed for its antifungal activity on 12 toxigenic strains of Fusarium graminearum isolated from barley and wheat. The susceptible metabolic pathways were assessed through the determination of structural compounds (glucosamine and ergosterol) and enzyme activity of the microorganisms' primary metabolism. The results indicate that phenolic... Tipo: Journal article Palavras-chave: Natural antifungal agent; Structural compounds; Enzymes. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000500012 Glycolysis and gluconeogenesis in the liver of catfish fed with different concentrations of proteins, lipids and carbohydrates Provedor de dados: 45 Autores: Melo,J.F.B.; Lundstedt,L. M; Inoue,L.A.K.; Metón,I.; Baanante,I.V.; Moraes,G. ABSTRACT The activities of enzymes from a number of metabolic pathways have been used as a tool to evaluate the best use of nutrients on fish performance. In the present study the catfish Rhamdia quelen was fed with diets containing crude protein-lipid-carbohydrate (%) as follows: treatment (T) T1: 19-19-44; T2: 26-15-39; T3: 33-12-33; and T4: 40-10-24. The fish were held in tanks of re-circulated, filtered water with controlled temperature and aeration in 2000L experimental units. The feeding experiment lasted 30 days. The following enzymes of the carbohydrate metabolism were determined: Glucokinase (GK), Phosphofructokinase 1 (PFK-1), Pyruvate kinase (PK), Fructose-1,6-biphosphatase 1 (FBP-1). The activities of 6 phosphogluconate dehydrogenase (6PGDH)... Tipo: Journal article Palavras-chave: Metabolism; Enzymes; Nutrition; Adaptation. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501251 Gongronema latifolium Modulates Rauwolfia vomitoria-Induced Behaviour and Histomorphology of the Cerebral Cortex Provedor de dados: 77 Autores: Ekong,Moses B; Peter,Aniekan I; Ekpene,Ubong U; Bassey,Enobong I; Eluwa,Mokutima A; Akpanabiatu,Monday I; Ekanem,Theresa B. Rauwolfia vomitoria (RV) has potent sedative effect, which may result in severe unpleasant consequences if not controlled. This necessitated this study on the effect of Gongronema latifolium (GL) on RV-induced behaviour, biochemical activities, and histomorphology of the cerebral cortex. Eighteen male Wistar rats of average weight 266 g were grouped into three (1-3). Group 1 was the control administered 0.5 mL of Tween®20, while groups 2 and 3 were administered 150 mg/kg of RV, and a combination of 150 mg/kg of RV and 200 mg/kg of GL (RV+GL), respectively for seven days. Twelve hours after treatments, open field neurobehavioral test was carried-out and the animals euthanized. Their sera were analyzed, and their cerebral cortices routinely processed by... Tipo: Journal article Palavras-chave: Rauwolfia vomitoria; Gongronema latifolium; Behavior; Enzymes; Lipid; Cerebral cortex; Histomorphology. Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100013 Groundnut bacterial wilt: past, present, and future Provedor de dados: 30 Autores: V.K. Palavras-chave: Bacteria; Groundnuts; Pathogens; PCR; DNA; Enzymes; Genotypes; Oil crops; Mutants. Ano: 1994 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4181 Growth factors in Penaeid shrimp feeding Provedor de dados: 5 Autores: Guillaume, Julien; Cruz, R; Cuzon, Gerard; Wormhoudt Van, A; Revol, A. Some nutrients known as non essential in vertebrates could be essential in invertebrates and could be related to some "unknown growth factors" which were evidenced or suspected, mainly in mollusc or crustacean flesh or meal, by several authors working on different species of crustaceans. A set of experiments was carried out in our laboratory in order to isolate one of these factors found in squid meal. Tipo: Text Palavras-chave: Penaeus japonicus; Food absorption; Digestive system; Haemolymph; Amino acids; Glucose; Digestion; Enzymes; Experimental research; Feeding; Shrimp culture; Laboratory culture. Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/acte-1464.pdf Hymenoptera venom review focusing on Apis mellifera Provedor de dados: 80 Autores: Lima,P. R. de; Brochetto-Braga,M. R. Hymenoptera venoms are complex mixtures containing simple organic molecules, proteins, peptides, and other bioactive elements. Several of these components have been isolated and characterized, and their primary structures determined by biochemical techniques. These compounds are responsible for many toxic or allergic reactions in different organisms, such as local pain, inflammation, itching, irritation, and moderate or severe allergic reactions. The most extensively characterized Hymenoptera venoms are bee venoms, mainly from the Apis genus and also from social wasps and ant species. However, there is little information about other Hymenoptera groups. The Apis venom presents high molecular weight molecules - enzymes with a molecular weight higher than... Tipo: Journal article Palavras-chave: Hymenoptera; Venom; Enzymes; Peptides. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200002 Identificação de enzimas vegetais com atividade coagulante sobre o leite para possível utilização na fabricação de queijos de cabra. Provedor de dados: 119 Autores: RODRIGUES, R. C.; LAGUNA, L. E. 2001 Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Caprino; Leite de cabra; Coagulação; Extrato vegetal; Queijo; Tecnologia de alimento; Enzima; Goat milk; Plant extracts; Coagulation; Goat cheese; Enzymes. Ano: 2001 Identification of new Sphingomyelinases D in pathogenic fungi and other pathogenic organisms. Provedor de dados: 119 Autores: DIAS-LOPES, C.; NESHICH, I. A. P.; NESHICH, G.; ORTEGA, J. M.; GRANIER, C.; CHÁVEZ-OLORTEGUI, C.; MOLINA, F.; FELICORI, L. Sphingomyelinases D (SMases D) or dermonecrotic toxins are well characterized in Loxosceles spider venoms and have been described in some strains of pathogenic microorganisms, such as Corynebacterium sp. After spider bites, the SMase D molecules cause skin necrosis and occasional severe systemic manifestations, such as acute renal failure. In this paper, we identified new SMase D amino acid sequences from various organisms belonging to 24 distinct genera, of which, 19 are new. These SMases D share a conserved active site and a C-terminal motif. We suggest that the C-terminal tail is responsible for stabilizing the entire internal structure of the SMase D Tim barrel and that it can be considered an SMase D hallmark in combination with the amino acid... Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bioinformática; Esfingomielinas; Enzimas; Bioinformatics; Sphingomyelins; Enzymes. Ano: 2013 Indicadores biológicos em solos de propriedades rurais sob plantio direto e convencional na região de Rio Verde-GO. Provedor de dados: 14 Autores: MENDES, I. de C.; SCOPEL, E.; REIS JÚNIOR, F. B.; MARCHETTI, A. D. 0 Tipo: Folderes / Folhetos / Cartilhas (INFOTECA-E) Palavras-chave: Biologia do solo; Cerrado; Enzima; Atividade enzimática; Soil biology; Enzymes; Enzymic activity. URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/567690 Influence of carbon source, pH, and temperature on the polygalacturonase activity of Kluyveromyces marxianus CCMB 322 Provedor de dados: 116 Autores: Oliveira,Rodrigo de Queiroz; Valasques Junior,Gildomar Lima; Uetanabaro,Ana Paula Trovatti; Koblitz,Maria Gabriela Bello; Góes-Neto,Aristóteles; Rosa,Carlos Augusto; Assis,Sandra Aparecida de. Microbial pectinolytic enzymes are known to play a commercially important role in a number of industrial processes. Two kinds of yeast can be discerned regarding the production of enzymes. One group includes those which can produce enzymes in the absence of an inducer, and the other group comprises the yeasts that produce enzymes in the presence of an inducer. The objective of this study was to investigate the influence of pectic substances, glucose, pH, and temperature on the polygalacturonase activity by Kluyveromyces marxianus CCMB 322. The yeast was grown in a fermentation broth containing different concentrations of glucose and pectic substances. The polygalacturonase activity was determined by the DNS method, and the pH and temperature were optimized... Tipo: Journal article Palavras-chave: Yeasts; Enzymes; Thermostability; Pectic substances; Biotechnology. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000300012 Influence of dietary live yeast on European sea bass (Dicentrarchus labrax) larval development Provedor de dados: 5 Autores: Tovar Ramirez, Dariel; Zambonino, Jose-luis; Cahu, Chantal; Gatesoupe, Joel; Vazquez Juarez, R. Recent results have demonstrated that live yeast in diets improved gut maturation in sea bass larvae. In this study, we tested the absence and two levels of live yeast in sea bass larvae diets. Specimens were fed from time of mouth opening to 37 days after hatching a diet of 0%, 1.1%, or 5.7% wet weight of live yeast (Debaryomyces hansenii CBS 8339). Yeast incorporation improved survival 10%, and reduced malformed larvae. In groups fed 1.1% yeast, only 1% of larvae were malformed, compared to 14% in the control group. Final mean weight in groups fed 1.1% yeast was twice that of other groups. Activities and concentrations of mRNA trypsin and lipase were higher in the two groups fed yeast than in the control group, whereas activity and concentration of mRNA... Tipo: Text Palavras-chave: Polyamines; Lipase; Trypsin; Amylase; Enzymes; Gut maturation; Fish larvae. Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-831.pdf Influence of nitrogen sources on the enzymatic activity and grown by Lentinula edodes in biomass Eucalyptus benthamii. Provedor de dados: 119 Autores: PEDRI, Z. C.; LOZANO, L. M. S.; HERMANN, K. L.; HELM, C. V.; PERALTA, R. M.; TAVARES, L. B. B. Lignocelulose é o componente mais abundante do meio ambiente e recurso orgânico renovável no solo. Alguns fungos filamentosos têm desenvolvido a habilidade de degradar e utilizar celulose, hemicelulose e lignina como fonte de energia. O objetivo deste trabalho foi analisar o efeito de três fontes de nitrogênio (sulfato de amônio, nitrato de potássio e farelo de soja) na atividade enzimática de Lentinula edodes EF 50 utilizando como substrato serragem de E. benthamii. Foi aplicado um planejamento experimental de mistura com três repetições no ponto central constituído de sete tratamentos (T) de iguais concentrações em nitrogênio de sulfato de amônia, nitrato de potássio e farinha de soja cozida. Foram determinadas a atividade enzimática da avicelase,... Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Basidiomicetos; Planejamento experimental de mistura; Basidiomycetes; Design of experimental mixture; Biomassa; Enzima; Eucalyptus benthamii; Lentinula edodes; Basidiomycota; Biomass; Enzymes. Ano: 2015 URL: http://dx.doi.org/10.1590/1519-6984.03214 Influence of stationary and bioreactor cultivation on Lentinula edodes (berk) pegler lignocellulolitic activity Provedor de dados: 52 Autores: Regina,Magali; Broetto,Fernando; Giovannozzi-Sermanni,Giovanni; Marabotini,Rosita; Peranni,Claudio. This work aimed to study the stationary and periodically mixed culture of L. edodes to the production of lignocellulolitic enzymes activity. LE 95/17, LE 96/22 and Leax strains were incubated in 25 g of eucalyptus sawdust substrate in Erlenmeyer flasks in stationary culture at 25º C and in a bioreactor with four complete rotations daily at 25º C and 3% CO2. The samples were collected at 8, 11, 14, 17 and 20 days after the incubation. Oxidative and hydrolytic enzymes analyses were performed. Lignin peroxidase enzyme was not found in the lignolytic system for LE 95/17, LE 96/22 and Leax strains in the different incubation methods. The use of bioreactor could be a practicable system to induce the laccase activity for L22 and Leax and MnP activity for L17 and... Tipo: Journal article Palavras-chave: Shiitake; Enzymes; Agricultural residues. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200001 Integration of Corynebacterium dapA and Escherichia coli lysC M4 genes to enhance lysine synthesis in rice endosperm Provedor de dados: 34 Autores: Julian, Russel Sadaba. xiv, 56 leaves : ill. Thesis (M.S.) -- University of the Philippines at Los Baños Tipo: Thesis Palavras-chave: Rice; Lysine; Enzymes; Proteins; Linkage (genetics); Transgenic plants; Corynebacterium; Genes; Escherichia coli. Ano: 2005 URL: http://hdl.handle.net/123456789/365 Interaction between xylanase, glucose oxidase and ascorbic acid on the technological quality of whole wheat bread Provedor de dados: 65 Autores: Silva,Camila Batista da; Almeida,Eveline Lopes; Chang,Yoon Kil. ABSTRACT: This study aimed to verify the performance of xylanase and its interaction with oxidants agents (glucose oxidase and ascorbic acid) on the quality of whole wheat bread. The experiment was based on a central composite rotational design and the Response Surface Methodology was used to analyze the results. None of the xylanase, glucose oxidase or ascorbic acid concentrations within the studied range led to a significant difference in the specific volume. The highest moisture content and the lowest firmness values were reported in the bread with lower and intermediate levels of xylanase and larger amounts of glucose oxidase and ascorbic acid. This effect was observed mainly at the end of the storage period. A minimum amount of xylanase (from 33 to 63... Tipo: Journal article Palavras-chave: Whole wheat bread; Enzymes; Oxidants; Texture; Response surface methodology. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202249 Interaction with Soil Constituents Determines the Environmental Impact of Proteins Provedor de dados: 127 Autores: Quiquampoix,H. Tipo: Journal article Palavras-chave: Proteins; Enzymes; Clay-humic complexes. Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912008000400012 Interactive effects between supplemental ultraviolet-B radiation and heavy metals on the growth and biochemical characteristics of Spinacia oleracea L. Provedor de dados: 61 Autores: Shweta,Mishra; Agrawal,S.B. The impact of supplemental UV-B (sUV-B) radiation and heavy metals: Cd (68 µmol.kg-1 soil) and Ni (68 µmol.kg-1 soil) singly and in combination was studied on photosynthetic and non-photosynthetic pigments, metabolites, enzyme activities, biomass accumulation and the net primary productivity of spinach (Spinacia oleracea L. var. All Green), a common leafy vegetable plant. Both the stresses individually and in combination resulted in the reduction of photosynthetic pigments, ascorbic acid, and catalase activity, whereas a reverse trend was observed for anthocyanin, flavonoids and proline content, lipid peroxidation and peroxidase activity. The interactive effects of both the stresses were, however, less than additive. Combined treatment of sUV-B + Cd was... Tipo: Journal article Palavras-chave: Spinaicia oleracea; Biomass; Cadmium; Enzymes; Nickel; Pigments; Ultraviolet-B. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000200007 International Chickpea and Pigeonpea Newsletter Provedor de dados: 30 Autores: Jagdish. Palavras-chave: Chickpeas; Pigeonpeas; Enzymes; Genotypes; Integrated pest management; Grafting; Biochemical compounds; Grain legumes; Sowing date; Abiotic injuries. Ano: 2002 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4648 Invertase and sucrose synthase activities in coffee plants sprayed with sucrose solution Provedor de dados: 63 Autores: Silva,José Carlos da; Alves,José Donizeti; Alvarenga,Amauri Alves de; Magalhães,Marcelo Murad; Livramento,Dárlan Einstein do; Fries,Daniela Deitos. One management practice of which the efficiency has not yet been scientifically tested is spraying coffee plants with diluted sucrose solutions as a source of carbon for the plant. This paper evaluates the effect of foliar spraying with sugar on the endogenous level of carbohydrates and on the activities of invertase and sucrose synthase in coffee (Coffea arabica L.) seedlings with reduced (low) and high (normal) levels of carbon reserve. The concentrations used were 0.5 and 1.0% sucrose, and water as a control. The use of sucrose at 1.0% caused an increase in the concentration of total soluble sugars in depauperate plants, as well as increased the activity of the following enzymes: cell wall and vacuole acid invertase, neutral cytosol invertase and... Tipo: Journal article Palavras-chave: Coffea arabica L.; Sugar; Spray; Enzymes. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000200006 Isolation and enzyme bioprospection of endophytic bacteria associated with plants of Brazilian mangrove ecosystem. Provedor de dados: 119 Autores: CASTRO, R. A.; QUECINE, M. C.; LACAVA, P. T.; BATISTA, B. D.; LUVIZOTTO, D. M.; MARCON, J.; FERREIRA, A.; MELO, I. S. de; AZEVEDO, J. L. Abstract: The mangrove ecosystem is a coastal tropical biome located in the transition zone between land and sea that is characterized by periodic flooding, which confers unique and specific environmental conditions on this biome. In these ecosystems, the vegetation is dominated by a particular group of plant species that provide a unique environment harboring diverse groups of microorganisms, including the endophytic microorganisms that are the focus of this study. Because of their intimate association with plants, endophytic microorganisms could be explored for biotechnologically significant products, such as enzymes, proteins, antibiotics and others. Here, we isolated endophytic microorganisms from two mangrove species, Rhizophora mangle and Avicennia... Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Mangrove; Endophytes; Bacteria; Biotechnological potential; Enzymes; Bactéria; Enzima; Mangue; Endophytes; Bacteria; Mangrove forests; Enzymes. Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1003307 Isolation of lipase producing fungi from palm oil Mill effluent (POME) dump sites at Nsukka Provedor de dados: 52 Autores: Nwuche,Charles Ogugua; Ogbonna,James Chukwuma. In this study, twelve fungal lipase producing strains belonging to Aspergillus, Penicillium, Trichoderma and Mucor genera were isolated from palm oil mill effluent composts. The Aspergillus spp. were more frequent (42%) and was present in all the samples assayed. Mucor sp. was the least encountered (8.3%).The lipase producing profile showed that Trichoderma (8.07-8.24 u/mL) and Aspergillus (6.25 -7.54 u/mL) spp. were the highest lipase producers while Mucor (5.72 u/mL) was the least. Tipo: Journal article Palavras-chave: Enzymes; Lipases; Triglycerides; Esterification; Waste water treatment. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100015 Kunitz trypsin Inhibitor and phytic acid levels in conventional and genetically modified soybean seeds from Londrina and Ponta Grossa, South Brazil. Provedor de dados: 119 Autores: GALÃO, O. F.; CARRÃO-PANIZZI, M. C.; MANDARINO, J. M. G.; LEITE, R. S.; CLAUS, T.; VISENTAINER, J. V. Diferenças em níveis de inibidor de tripsina Kunitz e ácido fítico foram determinadas para cultivares de soja convencionais e transgênicas cultivadas em Londrina e Ponta Grossa, Sul do Brasil. Os níveis de inibidor de tripsina na região de Londrina e Ponta Grossa para variedades convencionais variaram de 14,56 mg g-1 (BRS 267) a 20,40 mg g-1 (BRS 261) e de 13,51 mg g-1 (BRS 232) a 19,65 mg g-1 (BRS 268), respectivamente, enquanto para as variedades transgênicas foram encontrados valores de 14,16 mg g-1 (BRS 242 RR) a 18,21 mg g-1 (BRS 255 RR) para Londrina e 13,68 mg g-1 (BRS 244 RR) a 18,73 mg g-1 (BRS 256 RR) para Ponta Grossa. A média de teor de ácido fítico em variedades convencionais e transgênicas de Londrina teve variação de 2,05 a 1,78 mg g-1,... Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Soja convencional; Soja transgêncica; Enzimas; Melhoramento genético; Fatores antinutricionais; Conventional soybean; Genetically modified soybean; Enzymes; Genetic improvement; Antinutritional factors. Ano: 2014 La bonamiose: caractérisation du parasite Bonamia ostreae et étude des ses interactions avec l'hôte, l'huître plate Ostrea edulis Provedor de dados: 5 Autores: Besnard-cochennec, Nathalie. La bonamiose, maladie due au protozoaire Bonamia ostreae a été détectée en 1979 pour la première fois en Bretagne au cours d'épisodes de mortalité. Depuis, cette maladie s'est propagée à tous les centres ostréicoles français, puis européens. Cette étude a pour objectif de décrire les compartiments du modèle B. ostreae/hémocyte de l'huître plates, Ostrea edulis et d'analyser leurs interactions in vivo et in vitro. La caractérisation enzymatique, antigénique et moléculaire du parasite a été réalisée. Ceci a permis de disposer d'une palette de réactifs pour la comparaison de ce parasite avec d'autres protozoaires. Ainsi, un parasite de l'huître creuse chinoise Crassostrea rivularis a té décrit, puis rapproché du genre Bonamia. La morphologie et l'équipement... Tipo: Text Palavras-chave: Huître plate; Ostrea edulis; Huître creuse chinois; Crassostrea rivularis; Bonamia ostreae; Enzymes; Résistance; Mécanisme de défense. Ano: 1997 URL: http://archimer.ifremer.fr/doc/00211/32198/30635.pdf La ecotoxicidad y el pasto japonesa (Leersia sp.) en la fitorremediación de un Gleysol contaminado con petróleo fresco, intemperizado y sulfatos. Provedor de dados: 32 Autores: Arias Trinidad, Alfredo. El estudio de ecotoxicidad y fitorremediación de un Gleysol (GL) contaminado con petróleo fresco (PF) e intemperizado (PI) y sulfatos solubles (S-SO4), se realizo para seleccionar indicadores de toxicidad biológicos, evaluar el potencial del pasto japonesa (PJ) en la descontaminación de estos suelos, la presencia de S-SO4 en su interacción con los hidrocarburos, las bacterias sulfato reductoras y actividad enzimática de la arilsulfatasa (ASA). Dos GLs se usaron para establecer los bioensayos (BIO). El BIO1 con nueve tratamientos [T1: 2 607 (biogénico), T2: 6 000, T3: 10 000, T4: 30 000, T5: 60 000, T6: 90 000, T7: 120 000. T8: 150 000 y T9: 180 000 mg kg-1 suelo seco de hidrocarburos totales de petróleo fresco (HTPF)]. El BIO2 con seis tratamientos [T1: 2... Palavras-chave: Petróleo; Sulfatos; Bacterias; Enzimas; Degradación; Maestría; Producción Agroalimentaria en el Trópico; Oil; Sulfates; Enzymes; Degradation. Ano: 2012 URL: http://hdl.handle.net/10521/1762 Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems Provedor de dados: 52 Autores: Yan,Xu; Souza,Maria Aparecida; Pontes,Marcela Z. R.; Vitolo,Michele; Pessoa Júnior,Adalberto. From analytical to commercial scale, aqueous two-phase systems have their application in the purification, characterization and study of biomaterials. In order to improve the selectivity of the systems, the biospecific affinity ligands were introduced. In the affinity partitioning aqueous two-phase system, have many enzymes been purified. This review discusses the partitioning of some enzymes in the affinity aqueous two-phase systems in regard to the different ligands, including reactive dyes, metal ions and other ligands. Some integration of aqueous two-phase system with other techniques for more effective purification of enzymes are also presented. Tipo: Journal article Palavras-chave: Affinity partitioning; Aqueous two-phase systems; Reactive dyes; Ligands; Enzymes. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400030 Malassezia pachydermatis: enzymes production in isolates from external ear canal of dogs with and without otitis Provedor de dados: 45 Autores: Coutinho,S.D.A. The relationship between production of enzymes and pathogenicity of Malassezia pachydermatis strains was studied. Thirty strains of M. pachydermatis isolated from dogs were used: 15 originating from animals with external otitis and 15 obtained from ears of asymptomatic dogs. The samples, obtained by introduction of a sterile swab into the auditory canal after cleaning the auricle with alcohol-ether solution, were seeded onto Sabouraud dextrose agar with chloramphenicol (100mg/l) and were incubated at 37&deg;C for up to two weeks. The colonies were identified according to their macro-and-micromorphology. All strains were investigated for the production of chondroitin-sulphatase, hyaluronidase, phospholipase and proteinase. Most of the strains showed a... Tipo: Journal article Palavras-chave: Dog; Malassezia pachydermatis; Enzymes; Otitis; Microbiota. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000800003 Manual of laboratory procedures for quality evaluation of sorghum and pearl millet Provedor de dados: 30 Autores: M. I. Palavras-chave: Sampling; Flours; Distilling; Husking; Grain; Sorghum; Enzymes; Millets; Bakery products. Ano: 1997 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4197 Mecanismos de tolerância da variedade de milho "Saracura" (BRS 4154) ao alagamento. Provedor de dados: 119 Autores: ALVES, J.D.; MAGLHAES, M.M.; GOULART, P. de F.P.; DANTAS, B.F.; GOUVEA, J.A. de; PURCINO, R.P.; MAGALHAES, P. C.; FRIES, D.D.; LIVRAMENTO, D.E. do; MEYER, L.E.; SEIFFERT, M.; SILVEIRA, T. Esta revisao tem por objetivo discutir mecanismos de tolerancia desenvolvidos por plantula da variedade de milho "Saracura" (BRS 4154), que possui, como principal caracteristica, tolerancia a periodos intermitentes de encharcamento do solo. Os primeiros estudos de analise de tolerancia a baixa disponibilidade de oxigenio no meio, sob condicoes controladas, em sala de crescimento, confirmaram essa caracteristica, ao revelar que as plantulas suportam ate quatro dias de hipoxia sem nenhum dano aparente. A partir dai, apresentaram na regiao do mesocotilo, uma regiao translucida, caracteristica de lise celular, a qual evoluiu para uma constricao, como o prolongamento do estresse, causando murcha, tombamento e morte das plantulas. Estudos posteriores revelaram... Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Milho; Hipoxia; Calcio; Parede celular; Enzimas; Tolerancia; Alagamento; Zea mays; Maize; Calcium; Cell wall; Enzymes; Flooding. Ano: 2002 Metabolic adjustments in Satanoperca aff. jurupari (Perciformes: Cichlidae) Provedor de dados: 74 Autores: Chippari-Gomes,Adriana R.; Leitão,Marco Aurélio B.; Paula-Silva,Maria de Nazaré; Mesquita-Saad,Lenise S. B. de; Almeida-Val,Vera Maria F. In this paper, we describe the enzyme levels and isozyme distribution in skeletal and heart muscle of Satanoperca aff. jurupari, (Cichlidae, subgroup Geophaginae). LDH and CS were measured in skeletal and heart muscle. Starch gel electrophoresis was used to determine the isozyme/allozyme patterns in different tissues; LDH, MDHs, PGM, PGI, ADH, G-6-PDH and SOD were screened for the numbers of loci, presence of alleles, and tissue specificity. The LDH/CS ratio in heart and skeletal muscle were 173.36 and 6.1, respectively, indicating anaerobic metabolism in the former and aerobic metabolism in the latter muscle. No inhibition by pyruvate (based on the ratios of LDH activity with 1 mM and 10 mM pyruvate) was detected in heart and skeletal muscle, indicating... Tipo: Journal article Palavras-chave: Cichlid; Plasticity; Metabolism; Enzymes; Amazon. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100005 Metabolic responses of channel catfish (Ictalurus punctatus) exposed to phenol and post-exposure recovery Provedor de dados: 42 Autores: Moraes,Fernanda D.; Rossi,Priscila A.; Figueiredo,Juliana S.L.; Venturini,Francine P.; Cortella,Lucas R.X.; Moraes,Gilberto. Metabolic adjustments were studied in channel catfish Ictalurus punctatus exposed to 1.5 mg L-1 of phe nol (10% LC50) for four days and recovered for seven days. Lower triacylglycerol (TGA) stores and increased muscle fat free acids (FFA) suggest fat catabolism in muscle. Remarkable liver FFA decrease (-31%) suggests liver fat catabolism as well. Increased muscular ammonia levels and ASAT (aspartate aminotransferase) and decreased plasma aminoacids suggest higher muscular amino acid uptake. Constant levels of glucose and increased liver glycogen stores, associated with lower amino acids in plasma, indicate gluconeogenesis from amino acids. This is supported by higher hepatic ALAT and ASAT. Higher hepatic LDH followed by lower plasma lactate may indicate... Tipo: Journal article Palavras-chave: Metabolic adjustments; Enzymes; Lipid stores; Intermediary metabolism; Recovery; Xenobiotic. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000300865 Microbial alterations of the soil influenced by induced compaction Provedor de dados: 90 Autores: Pupin,Breno; Freddi,Onã da Silva; Nahas,Ely. Compaction is one of the most destructive factors of soil quality, however the effects on the microbial community and enzyme activity have not been investigated in detail so far. The objective of this study was to evaluate the effects of soil compaction caused by the traffic of agricultural machines on the soil microbial community and its enzyme activity. Six compaction levels were induced by tractors with different weights driving over a Eutrustox soil and the final density was measured. Soil samples were collected after corn from the layers 0-0.10 and 0.10-0.20 m. The compaction effect on all studied properties was evident. Total bacteria counts were reduced significantly (by 22-30 %) and by 38-41 % of nitrifying bacteria in the soil with highest bulk... Tipo: Journal article Palavras-chave: Microorganisms; Enzymes; Corn. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832009000500014 Microbial enzymatic activity and thermal effect in a tropical soil treated with organic materials Provedor de dados: 63 Autores: Cenciani,Karina; Freitas,Sueli dos Santos; Critter,Silvana Auxiliadora Missola; Airoldi,Cláudio. Bacteria and fungi are the most active decomposers of organic materials in soil. They directly affect plant nutrient availability, and chemical and physical properties of soils. This investigation aimed at quantifying the effect of several organic materials on microbial activity of a Rhodic Eutrudox. Soil samples were incubated over a period of 91 days with the following organic materials: cattle manure (CM), earthworm humus (HM), and city sewage sludge from Barueri (BA) and Franca (FR). The activities of cellulase, protease and urease enzymes, the soil microbial carbon content (by fumigation-extraction method) and the exothermal effect were evaluated. Experimental design was randomized and arranged as factorial scheme five treatments x seven samplings... Tipo: Journal article Palavras-chave: Enzymes; Biomass carbon; Organic materials; Soil microorganisms; Microcalorimetry. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000600016 Molecular key to seven Brazilian species of Trichogramma (Hymenoptera: Trichogrammatidae) using sequences of the ITS2 region and restriction analysis Provedor de dados: 82 Autores: CIOCIOLA JR,AMÉRICO I.; ZUCCHI,ROBERTO A.; STOUTHAMER,RICHARD. Using the ITS2 sequences of the ribosomal DNA together with some restriction enzymes, a simple and precise molecular key to some Brazilian species of Trichogramma was created. This key is very easy to make and quick results can be obtained on the identification of this minute parasitoid (0.25 mm). The methodology presented is easily implemented and can be used to detect possible contaminations under laboratory rearings. Tipo: Journal article Palavras-chave: Insecta; DNA; Enzymes; Biological control. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2001000200008 Mycoparasitic nature of Bionectria sp. strain 6.21. Provedor de dados: 119 Autores: MELO, I. S. de; VALENTE, A. M. M. P.; KAVAMURA, V. N.; VILELA, E. S. D.; FAULL, J. L. Abstract: In this study, a Bionectria sp. strain isolated from citrus rhizosphere was evaluated for its potential in inhibiting the growth of Rhizoctonia solani and Pythium aphanidermatum. It was demonstrated that Bionectria sp. 6.21 inhibited the growth of P. aphanidermatum and R. solani. In dual cultures, however, the antagonist only parasitised R. solani. Regarding the assay involving P. aphanidermatum, a lack of mycoparasitic ability was demonstrated. Crude extract of Bionectria completely inhibited the mycelial growth of both fungi. It appears that the main mechanism involved in the antagonism of Pythium by Bionectria is through antibiotic production. The antagonistic fungus released extracellular secondary metabolites. The metabolites were found to... Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Atividade antifúngica; Antifungal activity; Bionectria sp; Enzymes; Plant diseases; Fungo; Fungo para controle biológico; Controle biológico; Rhizoctonia solani; Pythium aphanidermatum; Doença de planta; Fungi; Plant diseases and disorders; Biological control agents; Antifungal properties; Mycoparasits. Ano: 2014 Nitrogen Nutrition of Groundnut in Alfisols Provedor de dados: 30 Autores: P.T.C. Palavras-chave: Root nodulation; Groundnuts; Nitrogen fixation; Genotypes; Rhizobium; Bacteria; Nitrogen; Enzymes; Application methods; Legumes. Ano: 1990 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4084 Nutritional Balance of broilers fed diets containing two calcium levels and supplemented with different phytase levels Provedor de dados: 89 Autores: Donato,DCZ; Ribeiro,P de AP; Magalhães,JD; Polycarpo,G do V; Garcia,PDSR; Burbarelli,MF de C; Albuquerque,R de. The objective of this study was to evaluate the effect of diets containing different calcium and phytase levels on the nutritional balance of broilers.A total of 108 male AG Ross 308 broilers were used in each of the replicates phases used in the study: starter (1-21 days), grower (29-35 days) and finisher (36-42 days). A completely randomized experimental design in a 3 x 2 factorial arrangement, with three phytase levels (0, 600 or 1200 FTU/kg) and two calcium levels (0.94 and 0.66%; 0.84 and 0.59%; e 0.78 and 0.54% in the starter, grower, and finisher phases, respectively), totaling six treatments with six replicates each.The experimental feeds also contained reduced available phosphorus levels and minimum crude protein level.The method of total excreta... Tipo: Journal article Palavras-chave: Enzymes; Calcium; Broilers. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000400010 Oxidative enzymes activity in sugarcane juice as a function of the planting system Provedor de dados: 116 Autores: Marques,Tadeu Alcides; Rampazo,Érick Malheiros; Marques,Patricia Angélica Alves. In Brazil, the largest producer of sugarcane in the world, the industrial process transforms this crop into ethanol and/or granulated sugar. Some cultivars exhibit enzymatic browning in the extracted sugarcane juice at levels harmful to the manufacturing process of white granulated sugar. The objective of this study was to assess the effect of sugarcane straw used as soil coverage, the use of different planting systems, and treatments with hydrogel polymer on enzymatic activity. The cultivar RB 86 7515 was sampled for 8 months; the first sample was obtained by cutting the upper portion of the stalk at the internode, which was taken to the laboratory for determination of the enzymatic activity of polyphenoloxidase (PPO) and peroxidase (POD). The soil... Tipo: Journal article Palavras-chave: Saccharum; Straw coverage; Enzymes. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000100021 Pathogenic characteristics of yeasts isolated from vaginal secretion preserved under mineral oil Provedor de dados: 80 Autores: Severo Gomes,B; Souza Motta,CM; Lima,AN; Porto,ALF. In order to evaluate the pathogenicity of yeasts isolated from vaginal secretion of pregnant and non-pregnant women - stored in mineral oil at the URM Mycology Collection, Department of Mycology, Federal University of Pernambuco - 30 samples belonging to the genera Candida, Rhodotorula, Trichosporon, and Kloeckera, were studied regarding their pathogenic characteristics, ability to grow at room temperature (28°C ± 1°C), 37°C, and 42°C for 72 hours, and production of both phospholipase and proteinase. Results showed that all 30 isolates (100%) were able to grow at room temperature and 37°C, and that 17 samples (57%) were able to grow at 42°C. Evaluation of enzymatic activity showed protease activity in only two isolates (7%), namely C. maritima and C.... Tipo: Journal article Palavras-chave: Pathogenicity; Yeasts; Enzymes. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400013 Performance and morphology of intestinal mucosa of broilers fed mannan-oligosaccharides and enzymes Provedor de dados: 45 Autores: Oliveira,M.C.; Rodrigues,E.A.; Marques,R.H.; Gravena,R.A.; Guandolini,G.C.; Moraes,V.M.B. The performance and the morphology of intestinal mucosa of broilers fed mannan-oligosaccharides (MOS) and enzymes (E) from one to 21-day-old were evaluated using 750 one-day-old chicks, assigned to a 2 x 2 + 1 factorial design - two levels of MOS (0 and 0.1%), two levels of E (0 and 0.05%) plus an antibiotic positive control diet - performing five treatments of five replications each one. MOS x E interaction was significant for both duodenal (P<0.002 and P<0.002) and ileal (P<0.04 and P<0.05) perimeters and heights of villi, being the values lower in the mucosa of birds fed non-supplemented diets. MOS based-diet determined an increase on perimeter of jejunal villi (P<0.05). Compared with antibiotic treatment group, villi perimeter... Tipo: Journal article Palavras-chave: Broiler; Nutrition; Enzymes; Prebiotic. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200025 Performance of transgenic rice expressing C4 photosynthesis enzymes Provedor de dados: 34 Autores: Jiao, D. M.; Ling, L. L.; Zhang, B. J. p.26 Palavras-chave: Transgenic rice; C4 photosynthesis; Enzymes. Ano: 2007 URL: http://www.irri.org/publications/irrn/pdfs/vol32no1/Plant%20breeding-1.pdf Phosphatases and antioxidant enzymes as potential biochemical indicators of toxicity in aquatic non-target organisms. Provedor de dados: 119 Autores: DANTZGER, D. D.; JONSSON, C. M.; AOYAMA, H. 2014 Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Phosphatases; Antioxidant Enzymes; Biochemical Indicators; Antioxidants; Enzymes; Diflubenzuron. Ano: 2014 Physicochemical and enzimatic transformations of "eva" apples stored under modified atmosphere Provedor de dados: 64 Autores: Thomas,Ariela Betsy; Pires,Caroline Roberta Freitas; Lima,Luiz Carlos de Oliveira. The need for a permanent market supply of fresh fruit has generated great interest in postharvest techniques that can promote useful life and quality of the fruit. The aim of this study was to evaluate the efficiency of atmospheric modification using different polymeric films in maintaining postharvest quality and prolonging the shelf life of Eva (Mallus sp) apples. For this, fruits from the city of Barbacena - MG, selected and sanitized were packed in plastic trays and wrapped in the corresponding films. The films used were: polypropylene - 20 µm, low-density polyethylene - 14 µm and high-density polyethylene - 7 µm. Another group of fruits (control) was not submitted to a modified atmosphere. Subsequently, all the the packages were stored under... Tipo: Journal article Palavras-chave: Mallus sp; Packaging; Firmness; Enzymes. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542014000400008 Polyphenoloxidase and peroxidase in avocado pulp (Persea americana Mill.) Provedor de dados: 116 Autores: Vanini,Lucimara Salvat; Kwiatkowski,Angela; Clemente,Edmar. The aim of the present investigation was to evaluate the enzymatic activity of polyphenoloxidase and peroxidase in avocado pulps, from the Northwest area of Paraná-Brazil, in order to compare the varieties on their enzymatic activity for both, minimum and industrial processing. Enzymatic extracts were prepared from avocado pulp of Choquete, Fortuna and Quintal varieties, in green and ripe maturation stage. Thermal treatment was applied with temperatures 60, 65, 70, 75 and 80 °C. The enzymatic activities were determined by using spectrophotometer. A decline of polyphenoloxidase activity was observed in all of the varieties when both, temperature and time increased. Total inactivation of enzymes was not observed in the largest temperature. Fortuna and... Tipo: Journal article Palavras-chave: Persea americana Mill; Enzymes; Temperature; Maturation stage. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000200036 Potencial biotecnológico de actinobactérias para produção de celulase. Provedor de dados: 119 Autores: OLIVEIRA, J. C. M. D. de; COTTA, S. P. M.; MELO, I. G.; REIS, D. P. dos; LANA, U. G. de P.; MARRIEL, I. E. 2016 Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Enzima; Streptomyces; Amycolatopsis; Enzymes. Ano: 2016 Potencial biotecnológico de actinomicetos para produção de amilase. Provedor de dados: 119 Autores: OLIVEIRA, J. C. M. D. de; BOMBA, J. P.; SOUSA, J. C. da C. de; MELO, I. G.; OLIVEIRA, C. A. de; LANA, U. G. de P.; MARRIEL, I. E. 2016 Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Enzima; Streptomyces; Kitasatospora; Enzymes. Ano: 2016 Production of Rhodotorula glutinis: a yeast that secretes alpha-L-arabinofuranosidase Provedor de dados: 69 Autores: Martínez,Claudio; Gertosio,Cecilia; Labbe,Angelica; Pérez,Rafael; Ganga,Maria Angélica. Rhodotorula glutinis is a yeast that secretes the enzyme alpha-L-arabinofuranosidase (E.C. 3.2.1.55) into the culture medium and thus has an interesting biotechnological potential. To determine improved culture conditions of this organism, different factors of the culture media were evaluated such as the use of peptone as nitrogen source, salts composition, pH and growth temperature. Likewise, beet molasses and beet cosette were tested as industrial carbon sources to induce the production of the enzyme and how they influence the yeast growth. Based on these studies a culture medium is proposed for growth of this yeast in a continuous system. By assaying different dilution rates an average specific activity for the enzyme of 82.4 U/mg of protein was obtained. Tipo: Journal article Palavras-chave: Alpha-L-arabinofuranosidase yeast; Enzymes; Wine. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400009 Proposta conceitual de estudo para a integração e gerenciamento do uso de marcadores bioquímicos de contaminação ambiental. Provedor de dados: 119 Autores: CASTRO, V. L. S. S. de; JONSSON, C. M. Resumo: A agricultura sustentável prioriza o uso racional da terra e da biota. Para tanto, é necessária a compreensão da interação entre os insumos agrícolas e os ecossistemas. Porém, há carência de métodos de fácil acesso para a verificação de contaminação ambiental por muitos desses produtos, como os agrotóxicos e de suas misturas. A aplicação de métodos alternativos no monitoramento de áreas degradadas pode gerar vantagens econômicas quando comparados à implementação de protocolos de análise que utilizam métodos de alto custo que demandam maior tempo de análise. Uma vez que a saúde ambiental pode ser avaliada por meio de estudos com indicadores biológicos, a abordagem aqui apresentada objetiva discutir alguns aspectos a respeito do monitoramento de... Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Monitoramento ambiental; Árvore de decisão; Enzimas; Indicadore biológico; Biomarcador; Agrotóxico; Impacto ambiental; Poluição ambiental; Pesticides; Environmental monitoring; Biomarkers; Enzymes. Ano: 2012 Pseudomonas spp. and P. fluorescens: population in refrigerated raw milk Provedor de dados: 65 Autores: Almeida,Kelly Molin de; Bruzaroski,Samera Rafaela; Zanol,Daniel; Melo,Marcela de; Santos,Joice Sifuentes dos; Alegro,Lina Casale Aragon; Botaro,Bruno Garcia; Santana,Elsa Helena Walter de. ABSTRACT: Raw milk samples were collected from cooling tanks (after they cooled for 48 h) in five dairy farms and the corresponding bulk tank (bulk milk transportation, BMT) when they arrived to the industry. Routine physical chemical analyzes and quantification of psychrotrophic ( Pseudomonas spp. and P. fluorescens ) and aerobic mesophilic (AM) populations were performed. Only relative density and titratable acidity values for samples of milk from three farms were in agreement to the quality parameters required by law. In the BMT, only the protein content has not reached the minimum value established by law, and counting was performed for AM (>105 colony forming units (CFU) mL-1) and psychrotrophic bacteria (2.8x106CFU mL-1). Pseudomonas spp.... Tipo: Journal article Palavras-chave: Psychrotrophic; Quality; Enzymes. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100451 Quality of fresh-cut avocado (Persea americana Mill.) stored under different temperatures Provedor de dados: 64 Autores: Pinheiro,Ana Carla Marques; Vilas Boas,Eduardo Valério de Barros; Silva,Lucas Carvalho e; Alves,Alessandra de Paiva; La Selva,Marcelo; Chitarra,Adimilson Bosco. The goal of this work was to evaluate the effect of different storage temperatures on the quality maintenance of fresh-cut 'Fortuna' avocado (Persea americana Mill.). The fruit was selected, washed and sanitized with sodium hypochlorite solution (190 ppm total residual chlorinee) for 15 minutes. After that, the tip was cut and the fruit was also cut in halves, peeled and the pit removed, manually. Then the halves were immersed in 0.5% calcium chlorite + 0.5% cysteine solution for 2 minutes, to avoid darkening and packed in rigid plastic covered with 20 micrometer thick plastic wrap (PVC film) with a permeability to O2, CO2 and water vapor of 15,000, 80,000 and 390 cm³/m²/day, respectively. The product was stored under 3 different temperatures 0, 5, 10ºC... Tipo: Journal article Palavras-chave: Appearance; Acceptability; Enzymes; Firmness; Avocado; Persea Americana. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542009000400021 Relations between bacterial extracellular enzyme activities and heterotrophic substrate uptake in a brackishwater environment. Provedor de dados: 5 Autores: Hoppe, H. Extracellular enzymes mediate the decomposition of polymeric organic compounds in natural waters. In many cases these enzymes comprise a component linked to the bacterial fraction of the aquatic community and react in close association with these cells. An annual survey of microbial activities in a brackish water fjord exhibited an excellent correlation between V sub(m) of proteases (ability to split the substrate analogue methylumbelliferyl-leucine) and V sub(m) as well as T sub(R) for leucine uptake by microorganisms. Hydrolysis rates (H sub(R)) for the decomposition of naturally occurring competitive substrate analogues of the model substrates (methylumbelliferyl-(MUF)-leucine, MUF- alpha -D-glucose, MUF-N-acetyl-glucosamine, MUF-phosphate) drastically... Tipo: Text Palavras-chave: Biogeochemistry; Fjords; Bacteria; Brackishwater environment; Seasonal variations; Dissolved organic matter; Interspecific relationships; Hydrolysis; Enzymes. Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-952.pdf ROLE OF ENZYMES IN THE REMEDIATION OF POLLUTED ENVIRONMENTS Provedor de dados: 126 Autores: Rao,M.A; Scelza,R; Scotti,R; Gianfreda,L. Environmental pollution is growing more and more due to the indiscriminate and frequently deliberate release of hazardous, harmful substances. Research efforts have been devoted to develop new, low-cost, low-technology, eco-friendly treatments capable of reducing and even eliminating pollution in the atmosphere, the hydrosphere and soil environments. Among biological agents, enzymes have a great potentiality to effectively transform and detoxify polluting substances because they have been recognized to be able to transform pollutants at a detectable rate and are potentially suitable to restore polluted environments. This brief review will examine some classes of pollutants and enzymes capable of transforming them effectively into innocuous products.... Tipo: Journal article Palavras-chave: Pollutants; Enzymes; Environmental pollution; Bioremediation. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162010000100008 Screening of actinomycetes from earthworm castings for their antimicrobial activity and industrial enzymes Provedor de dados: 58 Autores: Kumar,Vijay; Bharti,Alpana; Negi,Yogesh Kumar; Gusain,Omprakash; Pandey,Piyush; Bisht,Gajraj Singh. Actinomycetes from earthworm castings were isolated and screened for their antimicrobial activity and industrial enzymes. A total of 48 isolates were obtained from 12 samples of earthworm castings. Highest numbers of isolates were recovered from forest site (58.33 %) as compared to grassland (25%) and agricultural land (16.66%). The growth patterns, mycelial coloration of abundance actinomycetes were documented. The dominant genera Identified by cultural, morphological and physiological characteristics were Streptomyces (60.41%) followed by Streptosporangium (10.41%), Saccharopolyspora (6.25%) and Nocardia (6.25%). Besides these, other genera like Micromonospora, Actinomadura, Microbispora, Planobispora and Nocardiopsis were also recovered but in low... Tipo: Journal article Palavras-chave: Antimicrobial activity; Enzymes; Earthworm castings; Actinomycetes. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100022 SERUM BIOCHEMICAL CHANGES IN EXPERIMENTAL GAMBIAN TRYPANOSOMOSIS I. ENZYMES AND ELECTROLYTES Provedor de dados: 19 Autores: Abenga, J. N.; Anosa, V. O. Palavras-chave: Gambian trypanosomosis; Enzymes; Electrolytes; Trypanosoma brucei gambiense; Monkeys. Ano: 2004 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/643 Studies of chemical and enzymatic characteristics of Yacon (Smallanthus sonchifolius) and its flours Provedor de dados: 116 Autores: Pereira,Juciane de Abreu Ribeiro; Barcelos,Maria de Fátima Píccolo; Pereira,Michel Cardoso De Angelis; Ferreira,Eric Batista. Due to the importance of studies on yacon related to health, its in natura pulp, in natura peel, pulp flour, and peel flour were chemically analyzed in terms of its centesimal composition, specific minerals, total dietary fiber and fractions, pH, total soluble solids, total titratable acidity, tannins, oxalic acid, and nitrate. The polyphenoloxidase and peroxidase enzymatic activities were evaluated for in natura pulp and peel only. Yacon pulp and peel flour presented average yield of 7.94% and 10.86%, respectively. The in natura pulp presented a higher moisture and carboydrate content and lower lipid, protein, total dietary fiber, and ash than those of the peel flour. The same pattern was observed for pulp flour when compared to peel flour. The highest... Tipo: Journal article Palavras-chave: Dietary fiber; Antinutritional factors; Enzymes. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000100012 Temperature-dependent alterations in metabolic enzymes and proteins of three ecophysiologically different species of earthworms Provedor de dados: 52 Autores: Tripathi,G; Kachhwaha,N.; Dabi,I.; Bandooni,N. The effects of varying temperatures (12 - 44° C) on the specific activity of cytoplasmic malate dehydrogenase ((cMDH), mitochondrial malate dehydrogenase (mMDH) and lactate dehydrogenase (LDH) of some earthworms (Metaphire posthuma, Perionyx sansibaricus and Lampito mauritii) were studied. The effects of different temperatures on supernatant and mitochondrial protein contents were also investigated. The specific activities of cMDH, mMDH and LDH of the earthworms decreased gradually as a function of increasing temperature from 12 to 44°C. Higher metabolic energy was needed to maintain the activity at low temperatures. Hence, the earthworms showed increased enzyme specific activity at low temperatures. However, the protein content increased upto 28°C.... Tipo: Journal article Palavras-chave: Enzymes; Proteins; Earthworms; Temperatures. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400017 The influence of processing and long-term storage on the antioxidant metabolism of acerola (Malpighia emarginata) purée. Provedor de dados: 119 Autores: OLIVEIRA, L. S.; RUFINO, M. do S. M.; MOURA, C. F. H.; CAVALCANTI, F. R.; ALVES, R. E.; MIRANDA, M. R. A. de. 2011 Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Pulp; Enzymes; Vitamin C; Postharvest; Frozen. Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/901053 The influence of processing and long-term storage on the antioxidant metabolism of acerola (Malpighia emarginata) purée Provedor de dados: 61 Autores: Oliveira,Luciana S.; Rufino,Maria S. M.; Moura,Carlos F. H.; Cavalcanti,Fabio R.; Alves,Ricardo E.; Miranda,Maria R. A. This study evaluated the effect of the processing and long-term storage on the antioxidant potential and activity of antioxidant enzymes of frozen purées from six acerola clones. Ripe acerolas from clones BRS 235, BRS 236, BRS 237, BRS 238, II47/1 and BRS 152 were harvested; the pulp was processed, packed in sealed polyethylene plastic bags and stored in a domestic freezer at -18°C for 11 months. Samples of each clone were analyzed on harvest/processing day and every 30 days after for bioactive antioxidant compounds, antioxidant enzyme activity and total antioxidant activity. Acerola purées presented a decrease of non-enzyme antioxidants and an increase of antioxidant enzymes activities, indicatives of a compensatory mechanism between enzymatic and... Tipo: Journal article Palavras-chave: Pulp; Enzymes; Vitamin C; Postharvest; Frozen. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000200007 Thermotolerant fermenting yeasts for simultaneous saccharification fermentation of lignocellulosic biomass Provedor de dados: 69 Autores: Choudhary,Jairam; Singh,Surender; Nain,Lata. Lignocellulosic biomass is the most abundant renewable source of energy that has been widely explored as second-generation biofuel feedstock. Despite more than four decades of research, the process of ethanol production from lignocellulosic (LC) biomass remains economically unfeasible. This is due to the high cost of enzymes, end-product inhibition of enzymes, and the need for cost-intensive inputs associated with a separate hydrolysis and fermentation (SHF) process. Thermotolerant yeast strains that can undergo fermentation at temperatures above 40°C are suitable alternatives for developing the simultaneous saccharification and fermentation (SSF) process to overcome the limitations of SHF. This review describes the various approaches to screen and develop... Tipo: Journal article Palavras-chave: Bioethanol; Biofuel; Enzymes; Genome shuffling; Lignocellulosic biomass. Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300013 Two related thrombin-like enzymes present in Bothrops atrox venom Provedor de dados: 56 Autores: Petretski,J.H.; Kanashiro,M.; Silva,C.P.; Alves,E.W.; Kipnis,T.L. This article describes the presence of two new forms of a thrombin-like enzyme, both with apparent molecular masses of 38 kDa, in Bothrops atrox venom. Both share the ability to cleave fibrinogen into fibrin and to digest casein. Both present identical Km on the substrate BApNA. Their N-terminal amino acid sequences are identical for 26 residues, sharing 80% homology with batroxobin and flavoxobin. Two groups of monoclonal antibodies (mAbs) raised against the purified enzyme forms recognized different epitopes of the putative corresponding enzymes present in B. atrox crude venom. On Western blotting analysis of B. atrox crude venom, mAbs 5DB2C8, 5AA10 and 5CF11, but not mAbs 6CC5 and 6AD2-G5, revealed two or more protein bands ranging from 25 to 38 kDa. By... Tipo: Journal article Palavras-chave: Venoms; Bothrops atrox; Enzymes; Thrombin-like enzymes; Blood coagulation system. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100005 Ultrastructure et activite de la glande salivaire de Patella safiana LMK (Mollusque, Gasteropode) Provedor de dados: 5 Autores: Benmeradi, S; Benmeradi, N. The ultrastructural study of the Patella safiana salivary gland reveals two types of cells: the dark cells containing round-shaped secretory vesicles of lamellar structure and the light cells which have homogenous and fibrous secretory granules that are star-shaped sometimes. The two types of cells have the cytological characteristics of high proteosynthetic activity cells. Tipo: Text Palavras-chave: Patella safiana; Gastropoda; Biochemical analysis; Secretory organs; Cytology; Cells; Marine molluscs; Enzymes; Ultrastructure. Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-1712.pdf Une utilisation des enzymes proteolytiques extraites des visceres de poissons: La coagulation du lait. Provedor de dados: 5 Autores: Guerard, F. Chronic shortages in rennet have led to the use of enzyme preparations from various biological origins and capable of undergoing a milk-clotting process similar to that catalyzed by calf rennet. A proteolytic enzyme extracted from the gastric mucosa of dogfish Scyliorhinus canicula exhibits a clotting activity and several physical properties, which render it suitable for utilization in cheese-making industry. Tipo: Text Palavras-chave: Scyliorhinus canicula; Food technology; Enzymes; Shark utilization; Fishery products. Ano: 1985 URL: http://archimer.ifremer.fr/doc/1985/publication-1781.pdf Use of Enzymatic Biosensors as Quality Indices: A Synopsis of Present and Future Trends in The Food Industry Provedor de dados: 117 Autores: Serna Cock,Liliana; Zetty Arenas,Ana María; Ayala Aponte,Alfredo. Biosensors are an important alternative in the food industry to ensure the quality and safety of products and process controls with effective, fast and economical methods. Their technology is based on a specific biological recognition element in combination with a transducer for signal processing. The use of enzymatic biosensor technology in food processing, quality control and on-line processes is promising compared to conventional analytical techniques, as it offers great advantages due to size, cost, specificity, fast response, precision and sensitivity. This article reviews the development and use of some enzyme biosensors in the food industry, describes the most important application areas and analyzes the current situation and future possibilities.... Tipo: Journal article Palavras-chave: Biosensors; Enzymes; Analysis in food; Safety; Quality; Control processes. Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000200017 Utilização de complexo enzimático em rações para codornas de corte - doi: 10.4025/actascianimsci.v33i3.11216 Provedor de dados: 2 Autores: Iwahashi, Alexandre Shigueki; Universidade Estadual de Maringá; Furlan, Antônio Claudio; Universidade Estadual de Maringá; Scherer, Carina; Universidade Estadual de Maringá; Ton, Ana Paula Silva; UEM; Lorençon, Leticia; UEM; Scapinello, Cláudio; Universidade Estadual de Maringá. Foram conduzidos dois experimentos com o objetivo de avaliar os efeitos da suplementação enzimática das rações à base de milho e farelo de soja sobre o desempenho e metabolizabilidade dos nutrientes em codornas de corte. Os tratamentos foram: controle positivo para atendimento das exigências; controle positivo suplementado com complexo enzimático e controles negativos suplementados reduzidos em 2 e 4% nos teores de energia metabolizável e aminoácidos. Na fase inicial, observou-se aumento no consumo de ração (p < 0,05) no tratamento controle negativo reduzido em 4% na energia metabolizável e aminoácidos em comparação aos tratamentos controle positivos. Na fase de crescimento o consumo de ração, conversão alimentar e rendimento de carcaça não diferiram (p... Tipo: Pesquisa de campo ; análise laboratorial Palavras-chave: 5.04.03.02-8 balanço de nutrientes; Carboidrases; Coturnix coturnix sp.; Desempenho; Enzimas; Rendimento de carcaça nutrient balance; Carbohydrases; Coturnix coturnix sp.; Performance; Enzymes; Carcass yield. Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/11216 Variations des activites digestives en fonction des facteurs du milieu chez les crustaces Provedor de dados: 5 Autores: Galgani, Francois; Benyamin, Y; Vanworhmoudt, A; Cecaldi, J. Proteolytic enzymes of hepatopancreas of six species of peneids (Penaeus kerathurus, P. japonicus, P. monodon, P. stylirostris, P. merguiensis and P. vannamei ) have been studied. Some of their physiological variations have been characterised. Increase in acclimation temperature of crustaceans enhances increase of specific activities of proteases. Variations of salinity does not modify specific activities, nor quantities of trypsin, nor electrophoretic patterns of these different proteases. Tipo: Text Palavras-chave: Penaeus kerathurus; Penaeidae; Malacostraca; Diets; Growth; Larval development; Life cycle; Acclimation; Environmental effects; Digestive glands; Enzymatic activity; Salinity effects; Temperature effects; Enzymes; Endocrinology; Digestion; Développement larvaire; Croissance; Cycle d'intermue; Carboxypeptidase A and B; Leucine aminopeptidase; Chymotrypsin; Trypsine; Enzymes digestives; Homarus ganmarus; Palaemon serratus; P. vannamei; P. merguiensis; P. stylirostris; P. monodon; P. japonicus; Penaeus keratkurus. Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1195.pdf VCP1 PROTEASE DETECTION IN Pochonia chlamydosporia var. CATENULATA STRAIN IMI SD 187 Provedor de dados: 100 Autores: Peteira-Delgado,B; Hidalgo-Díaz,L. The mode of action of Pochonia as a biological control agent is the penetration by means of the hyphae into the target nematode eggs. The egg shell of nematodes has an outer vitelline membrane composed mainly by proteins. Thus, hyphal penetration is the result of a physic pressure and the specific hydrolytic activity of some enzymes such as proteases and chitinases. The most important protease studied in Pochonia chlamydosporia var. chlamydosporia is VCP1. The aim of this work was to detect and characterize the protease VCP1 in a strain of P. chlamydosporia var. catenulata, a potential biological control agent for root-knot nematodes. The specific activity of this protease was tested on different culture media supplemented with protein inductors using... Tipo: Journal article Palavras-chave: Nematode parasites; RFLP; Enzymes; Protease. Ano: 2009 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522009000300006 What mineral and vitamin levels to recommend in swine diets? Provedor de dados: 96 Autores: Gaudré,Didier; Quiniou,Nathalie. Minerals, including trace-elements, and vitamins are currently introduced into the pig diet in order to obtain suitable performances for the modern pig genotype bred in confinement. Most of the mineral and vitamin contents of cereals and usual protein compounds are characterized by their poor and variable availability. Supplemental diets are then usually needed. This paper, based on literature data, reviews the pig requirement for minerals and vitamins in order to ensure economic and profitable performance. Recent research has focused on phosphorus due to environmental considerations. The widespread use of microbial phytase has decreased the need for inorganic phosphates. Cereals are particularly deficient in calcium and sodium, consequently limestone and... Tipo: Journal article Palavras-chave: Environment; Enzymes; Feeding; Meat quality; Modern genotype; Nutritional requirements. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009001300019 Активность ферментов пероксидного комплекса тканей мидии Mytilus galloprovincialis Lam в норме и в условиях естественного окислительного стресса Provedor de dados: 13 Autores: Гостюхина, О. Л.; Солдатов, А. А.; Головина, И. В. Исследовано содержание продуктов перекисного окисления липидов (ПОЛ) и состояние ферментативного пероксидного комплекса (ПК) (глутатионпероксидазы (ГП), каталазы и пероксидазы) в гепатопанкреасе, жабрах и ноге черноморского моллюска Mytilus galloprovincialis Lam. в условиях физиологического покоя и нереста. Последнее рассматривали как модель естественного окислительного стресса. Изучали особей двух цветовых морф - черной и коричневой. Выявлена тканевая специфика активностей ферментов и распределения продуктов ПОЛ в состоянии нормы, а также особенности ответных реакций тканей моллюсков обеих морф при действии окислительной нагрузки. Наиболее чувствительной к действию перекисных продуктов в условиях естественного окислительного стресса оказались особи черной... Tipo: Journal Contribution Palavras-chave: Ферменты; Каталаза; Пероксидаза; Глутатионпероксидаза; Перекисное окисление липидов; Окислительный стресс; Мидии; Цветовые морфы; Нерест; Enzymes; Catalase; Peroxidase; Glutathione peroxidase; Lipid peroxidation; Spawning; Oxidative stress; Mussels; Color morphs Spawning Enzymes Lipids Spawning Enzymes Lipids http://aims.fao.org/aos/agrovoc/c_14490 http://aims.fao.org/aos/agrovoc/c_2603 http://aims.fao.org/aos/agrovoc/c_4362. Ano: 2005 URL: http://repository.ibss.org.ua/dspace/handle/99011/226 Действие цинка на активность супероксиддисмутазы и каталазы у Spirulina platensis (Nordst) geitl. Provedor de dados: 13 Autores: Шахматова, О. А. Изучали влияние двухвалентного цинка на активность антиоксидантных ферментов супероксиддисмутазы и каталазы у одноклеточной синезеленой водоросли Spirulina platensis. Двухвалентный цинк в концентрациях 0,09 - 9,0 мг/л угнетающе действовал на активность антиоксидантных ферментов супероксиддисмутазы и каталазы у спирулины. Зависимость активности каталазы и супероксиддисмутазы от концентрации цинка выражалась формулой y=а/(1+bх), где a=0,52 и b=5,28 для каталазы и а=0,49 и b=4,79 для супероксиддисмутазы, что подтверждает факт согласованности действия данных энзимов. Tipo: Journal Contribution Palavras-chave: Enzymes; Protein; Zn; Антиоксидантные ферменты; Спирулина Enzymes Enzymes http://aims.fao.org/aos/agrovoc/c_2603. Ano: 2002 URL: http://repository.ibss.org.ua/dspace/handle/99011/390