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Consulta: subject:"Bivalve" Registros recuperados: 117 Data/hora: 09/06/2017 09:59:33 Bacterial problems associated with scallop Pecten maximus larval culture Provedor de dados: 5 Autores: Nicolas, Jean-louis; Corre, S; Gauthier, Gilles; Robert, Rene; Ansquer, Dominique. Scallop Pecten maximus larvae have been cultured at the Argenton and Tinduff (Brittany, France) hatcheries with antibiotic treatment (chloramphenicol at 8 ppm) for 15 yr. Without treatment, outbreak of disease has normally occurred between Day 12 and Day 19 or sometimes earlier. A bacteriological study of larvae reared with and without antibiotic was performed over a 4 yr period. Among the collected strains, 2 clusters (C and F) of vibrios were present at high densities only in larvae cultured without treatment. One cluster (C) was routinely isolated over the 4 yr of study, while the other (F) was collected only in the third year. Their virulence with respect to scallop larvae and their lack of infectivity with respect to oyster larvae were demonstrated in... Tipo: Text Palavras-chave: Larvae; Bivalve; Vibrio; Scallop. Ano: 1996 URL: http://archimer.ifremer.fr/doc/00090/20077/17716.pdf Hermaphroditism in Marine Mussel Perumytilus purpuratus (Lamarck, 1819), (Mollusca: Mytilidae) Provedor de dados: 77 Autores: Montenegro Villalobos,Diana; Olivares Paz,Alberto; González,María Teresa. Marine bivalves are essentially gonochoric species. However, some occasional hermaphrodites specimens can be found. A histological description of two (among 179) hermaphrodites of the marine mussel Perumytilus purpuratus (Lamarck, 1819) that were collected in San Jorge Bay (24S) is given. The low proportion of hermaphrodites suggests that P. purpuratus hermaphroditism could be an accidental phenomenon; nevertheless other causes are also discussed in the present study. Tipo: Journal article Palavras-chave: Bivalve; Hermaphroditism; Perumytilus purpuratus. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200038 Viruses of bivalve shellfish Provedor de dados: 5 Autores: Renault, Tristan. Bivalve molluscs are filter feeders and as a consequence they may bioaccumulate in their tissues viruses that infect humans and higher vertebrates. However, there have also been described mortalities of bivalve molluscs associated with viruses belonging to different families. Mass mortalities of adult Portuguese oysters, Crassostrea angulata, among French livestocks (between 1967 et 1973) were associated with irido-like virus infections. Herpesviruses were reported in the eastem oyster, Pacific oyster and European flat oyster and lately in scallops and clams. Disseminated neoplasia, a proliferative cell disorder of the circulatory system of bivalves, although of uncertain aetiology, has been suggested to be caused by retroviral infections. Other viruses... Tipo: Text Palavras-chave: Infection; Virus; Mollusc; Bivalve; Infection; Virus; Mollusque; Bivalve. Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2925.pdf Contrôle de la ploidie par imagerie numérique dans des expériences d'induction de la triploidie chez les mollusques bivalves Provedor de dados: 5 Autores: Gerard, Andre; Peignon, Jean-marie; Chagot, Dominique. Individual determination of ploidy level in experimentally induced triploid bivalve molluscs using image cytometry. The sexual maturation in bivalve molluscs causes decreased growth and sometimes increased mortalities. These drawbacks can be avoided by genetic sterilization (triploidy). Triploïdy induction require a egg treatment and a ploidy control at each generation. The ploidy level is usually determined by counting the chromosomes, a technique which is relatively easy for the trochophora stage and for juveniles measuring from 5 mm to 3 cm only. ln order to be able to follow up the evolution of the ploidy rate during any stage of animallife, we have developed an estimation method based on image cytometry. Individual DNA content is determined by... Tipo: Text Palavras-chave: Image cytometry; Caryology; Triploidy; Bivalve; Imagerie numérique; Caryologie; Triploïdie; Bivalve. Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-3202.pdf Does bivalve mucus favour the growth of their main food source, microalgae? Provedor de dados: 5 Autores: Cognie, B; Barille, Laurent. Bivalves, by releasing inorganic nutrients, can stimulate the growth of the primary producers on which they feed. Dissolved excretion and mineralization of biodeposits (pseudofaeces and faeces), two indirect processes related to their feeding activity, have already been identified. This study investigated whether microalgal growth is also dependent on the mucus secreted by bivalves and rejected with their biodeposits. An experimental study based on algal growth tests was conducted to determine whether the mucus coating the pseudofaeces and faeces of the oyster Crassostrea gigas (Thunberg) could stimulate the growth of nine marine microalgae: a Prasinophyceae [Tetraselmis suecica (Butcher)] and eight Diatomophyceae [Amphora sp. (Ehrenberg), Chaetoceros sp.... Tipo: Text Palavras-chave: Bivalve; Microphyte; Mucus; Fèces; Pseudofèces; Bivalve; Microalgae; Mucus; Faeces; Pseudofaeces. Ano: 1999 URL: http://archimer.ifremer.fr/doc/00325/43607/43926.pdf Analise morfométrica das conchas e rendimento da carne de Anomalocardia brasiliana no estuário adjacente a Praia de Barra Grande, Cajueiro da Praia ? PI. Provedor de dados: 119 Autores: FREITAS, S. T.; FOGACA, F. H. dos S.; LEGAT, J. F. A.; BARROS, R. F. M.; PAMPLIN, P.A. Z. Apesar da elevada importância ambiental, econômica, social e cultural, não existem estudos voltados para a pesca de moluscos bivalves no litoral piauiense. Esse estudo teve como objetivo avaliar a morfometria e o rendimento de carne do bivalve marinho venerídeo Anomalocardia brasiliana. Exemplares de Abrasiliana foram coletados em cinco pontos distantes 200 metros entre si, em estuário próximo a Praia de Barra Grande-PI, durante a maré baixa de sizígia. A temperatura e salinidade da água foram medidas na margem dos pontos de coleta com utilização de sonda multiparâmetros. Em cada amostragem foi coletado sedimento para análise de pH e matéria orgânica. Os parâmetros abióticos foram semelhantes nos pontos de coleta. A análise morfométrica determinou valores... Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Agregação de valor; Marisqueira; Molusco bivalve. Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/handle/doc/871113 Monitoramento histopatológico de mexilhão Perna perna da Lagoa de Itaipu, Niterói, RJ Provedor de dados: 45 Autores: Lima,F.C.; Abreu,M.G.; Mesquita,E.F.M. Cortes histológicos de 120 mexilhões Perna perna (Linnaeus, 1758), coletados na Lagoa de Itaipu, Niterói, RJ, por um período de 12 meses, foram examinados quanto a presença de parasitas e lesões. Noventa exemplares apresentaram cinco diferentes processos patológicos: bucefalose, presença de protozoários Nematopsis sp., inclusões basofílicas não específicas, encapsulamentos parasitários e cistos epiteliais branquiais. Cinquenta animais mostravam duas ou mais patologias concomitantes. Tipo: Journal article Palavras-chave: Mexilhão; Perna perna; Bivalve; Parasita; Histopatologia. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000200013 PRIMER REGISTRO EN COLOMBIA DE CORBICULA FLUMINEA (MOLLUSCA: BIVALVIA: CORBICULIDAE), UNA ESPECIE INVASOR Provedor de dados: 50 Autores: De La Hoz Aristizábal,Maria V. The bivalve Corbicula fluminea Müller is recorded for the first time in Colombia, in the National Park Isla de Salamanca. This protected area includes freshwater channels and mixohaline lagoons influenced by waters of both the Magdalena River and the Caribbean Sea. This exotic bivalve occurs locally in freshwater channels close to the Magdalena River as well as in the latter, whereas another corbiculid native species, Polymesoda solida, is commonly found in the mixohaline lagoons. Densities of C. fluminea ranged from 10 to 100 ind/m2 along with low numbers of P. solida. Lengths ranged form 10 to 43 mm in a predominant muddy substrate. Local fishermen have stated that C. fluminea was established approximately in 1992; this clam is being artisanally... Tipo: Journal article Palavras-chave: Corbicula; Invasive bivalve; Channels; Colombia; Caribbean. Ano: 2008 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97612008000100011 Recolonization of experimental gaps by the mussels Brachidontes darwinianus and B. solisianus in a subtropical rocky shore Provedor de dados: 52 Autores: Tanaka,Marcel Okamoto. In this study, the recolonization speed of the mussels Brachidontes darwinianus and B. solisianus was compared to evaluate their responses to gaps opened by disturbances in the rocky shore. B. darwinianus was much faster than B. solisianus, rapidly closing gaps in the mussel bed. When they co-occurred, distribution patterns of these species were related not only with distinct physiological tolerances but also with responses to disturbance. B. darwinianus was more tolerant to low salinities, dominated estuarine zones generally occuring lower in the midlittoral, and presented a faster response to opened gaps. Tipo: Journal article Palavras-chave: Bivalve; Intertidal; Rocky shore; Zonation; Mytilidae. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100015 Space-time variation of the relative abundance of Limnoperna fortunei in deep zones of São Gonçalo Channel, Rio Grande do Sul, Brazil Provedor de dados: 76 Autores: Lopes,Michelle; Vieira,João. This work describes the spatial-temporal variation of the relative abundance and size of Limnoperna fortunei (Dunker, 1857) collected in São Gonçalo Channel through bottom trawl with a 0.5 cm mesh, at depths between 3 and 6 m. The estimative of mean relative abundance (CPUE) ranged from 2,425.3 individuals per drag (ind./drag) in the spring to 21,715.0 ind./drag in the fall, with an average of 9,515.3 ind./drag throughout the year. The estimated mean density of L. fortunei for the deep region of São Gonçalo Channel ranged from 1.2 to 10.3 ind./m², and it was recorded a maximum density of 84.9 ind./m² in the fall of 2008. The method of sampling using bottom trawl enabled the capture of L. fortunei under the soft muddy bottom of the channel, in different... Tipo: Journal article Palavras-chave: Freshwater invasion; Frequency of sizes; Golden mussel; Bivalve. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212012000400002 In vivo RNA interference in oyster -vasa silencing inhibits germ cell development Provedor de dados: 5 Autores: Fabioux, Caroline; Corporeau, Charlotte; Quillien, Virgile; Favrel, Pascal; Huvet, Arnaud. This study investigated the potential of RNA interference, which is technically challenging in bivalve mollusc species, to assess gene function in the oyster Crassostrea gigas. We designed dsRNA targeting the oyster vasa-like gene (Oyvlg), specifically expressed in oyster germ cells. In vivo injection of oyvl-dsRNA into the gonad provokes a knockdown phenotype corresponding to germ cell underproliferation and prematurely arrested meiosis througout the organ. The most severe phenotype observed is sterile. This knockdown phenotype is associated with a decrease in Oyvlg mRNA level of between 39% and 87%, and a strong reduction in OYVLG protein, to an undetectable level. Therefore, Oyvlg appears to be essential for germ cell development in Crassostrea gigas,... Tipo: Text Palavras-chave: Vasa; RNAi; Marine bivalve; Germline; Crassostrea gigas. Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6641.pdf An amylase gene polymorphism is associated with growth differences in the Pacific cupped oyster Crassostrea gigas Provedor de dados: 5 Autores: Prudence, Marie; Moal, Jeanne; Boudry, Pierre; Daniel, Jean-yves; Quere, Claudie; Jeffroy, Fanny; Mingant, Christian; Ropert, Michel; Bedier, Edouard; Van Wormhoudt, A; Samain, Jean-francois; Huvet, Arnaud. This study investigated the non-neutrality of genetic polymorphism in two alpha-amylase genes (AMYA and AMYB) in the oyster Crassostrea gigas. Bi-parental oyster families, bred to be polymorphic for markers in these genes, were monitored for growth and survival for 1 year under standard culture conditions in two French production sites. Within-family genotype frequencies indicated that the two amylase genes were closely linked (c. 1.7 cM). Within two of three families, significant differences in growth were observed between genotypes at one of the two production sites, suggesting that this polymorphism is not neutral and might be under selection because of its role in digestive function. Estimated daily yields were different between amylase genotypes,... Tipo: Text Palavras-chave: Growth; Genotype; Digestive enzyme; Bivalve; Alpha amylase. Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1691.pdf Cell separation by centrifugal elutriation Provedor de dados: 5 Autores: Bachere, Evelyne; Chagot, Dominique; Grizel, Henri. There is an ongoing need to isolate and purify ceIls in bivalve mollusc disease research. Many separation techniques differentiate cells solely by a single factor and usually require additives in the medium that may damage live cells. Centrifugai elutriation is a technique that separates particles by size and density simultaneously and can be conducted in physiological media without additives. Living cells can be separated without chemical damage and, because the procedure can be conducted aseptically, cells can be maintained in vitro after elutriation. A description of the technique, methods to determine flow rates and rotor speeds, and a preliminary separation of oyster hyalinocytes is presented Tipo: Text Palavras-chave: Bivalve mollusc; Centrifugal elutriation; Cell separation. Ano: 1988 URL: http://archimer.ifremer.fr/doc/1988/publication-3118.pdf Evidence of a Biological Control over Origin, Growth and End of the Calcite Prisms in the Shells of Pinctada margaritifera (Pelecypod, Pterioidea) Provedor de dados: 5 Autores: Cuif, Jean-pierre; Burghammer, Manfred; Chamard, Virginie; Dauphin, Yannicke; Godard, Pierre; Le Moullac, Gilles; Nehrke, Gernot; Perez-huerta, Alberto. Consistently classified among the references for calcite simple prisms, the microstructural units that form the outer layer of the Pinctada margaritifera have been investigated through a series of morphological, crystallographical and biochemical characterizations. It is often said that the polygonal transverse shape of the prisms result from the competition for space between adjacent crystals. In contrast to this classical scheme the Pinctada prisms appear to be composed of four successive developmental stages from the concentrically growing disks on the internal side of the periostracum to the morphological, structural and compositional changes in both envelopes and mineral components at the end of the prisms. These latest structural and compositional... Tipo: Text Palavras-chave: Biomineralization; Bivalve shell; Microstructure; Calcite prisms. Ano: 2014 URL: http://archimer.ifremer.fr/doc/00249/36039/34574.pdf Heat shock protein 90 of Bonamia ostreae: characterization and possible correlation with infection of the flat oyster, Ostrea edulis Provedor de dados: 5 Autores: Prado Alvarez, Maria; Chollet, Bruno; Couraleau, Yann; Morga, Benjamin; Arzul, Isabelle. In this study we described the cytosolic HSP90 of Bonamia ostreae, an intracellular parasite of Ostrea edulis haemocytes. The complete open reading frame was assembled by Rapid Amplification cDNA Ends reactions on cDNA of B. ostreae infected haemocytes. HSP90 amplification was corroborated in infected oysters and B. ostreae purified cells. The functionality of the HSP90, studied by inhibitory assays with radicicol, suggests that this protein may play a role in haemocyte invasion. Our results inform the molecular basis that governs B. ostreae-O. edulis interactions. Tipo: Text Palavras-chave: Bivalve haemocytes; Gene; Haplosporidia; Pathogen; Protist; Radicicol. Ano: 2013 URL: http://archimer.ifremer.fr/doc/00137/24799/22878.pdf Quelques paramètres permettant de suivre la production organique d'un mollusque bivalve au cours d'un cycle saisonnier Provedor de dados: 5 Autores: Bodoy, Alain; Masse, H. Some parameters measuring the production of organic matter, in field conditions, by a population of the bivalve mollusc Donax trunculus have been stu-died. Those studies which are easily done when the population is composed of long lived species become difficult in Mediterranean Sea where the populations are instable and composed of short-lived species. A large qualitative sample has been made every month on which the inter-pretation of cohort succession has been done using the graphical method of CASSIE (1954) which is an useful mean to determine the modal class-size. The monthly values of the weight/size relationship, during a year, show important fluctuations indicating that size increment, tissue weight increment, gamet production and release are often... Tipo: Text Palavras-chave: Production; Mollusques bivalves; Production; Molluscs bivalve. Ano: 1979 URL: http://archimer.ifremer.fr/doc/00306/41696/40907.pdf Association among growth, food consumption-related traits and amylase gene polymorphism in the Pacific oyster Crassostrea gigas Provedor de dados: 5 Autores: Huvet, Arnaud; Jeffroy, F; Fabioux, C; Daniel, Jean-yves; Quillien, Virgile; Van Wormhoudt, A; Moal, Jeanne; Samain, Jean-francois; Boudry, Pierre; Pouvreau, Stephane. To examine further a previously reported association between amylase gene polymorphism and growth in the Pacific oyster Crassostrea gigas, ecophysiological parameters and biochemical and molecular expression levels of alpha-amylase were studied in Pacific oysters of different amylase genotypes. Genotypes that previously displayed significantly different growth were found to be significantly different for ingestion and absorption efficiency. These estimated parameters, used in a dynamic energy budget model, showed that observed ingestion rates (unlike absorption efficiencies) allowed an accurate prediction of growth potential in these genotypes. The observed association between growth and amylase gene polymorphism is therefore more likely to be related to... Tipo: Text Palavras-chave: Ingestion; Gene expression; Bivalve; Alpha amylase. Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-5165.pdf Apoptose et infections à virus de type herpès chez les bivalves marins : détection de séquences codant pour des protéines inhibitrices de l'apoptose Provedor de dados: 5 Autores: Didier, Yohann. Tipo: Text Palavras-chave: PCR; Apoptosis; Marine bivalve; Herpes like virus. Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/rapport-1768.pdf Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) Provedor de dados: 5 Autores: Fuhrmann, Marine; Petton, Bruno; Quillien, Virgile; Faury, Nicole; Morga, Benjamin; Pernet, Fabrice. Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-related effects on the pathogen itself. To obtain donors for OsHV-1 transmission, we transferred laboratory-raised oysters to an estuary during a disease outbreak and then back to the laboratory. Oysters that tested OsHV-1 positive were placed in seawater tanks (35‰, 21°C). Water from these tanks was used to infect naïve oysters in 2 experimental setups: (1) oysters acclimated or non-acclimated to a salinity of 10, 15, 25 and 35‰ and (2) oysters acclimated to a salinity of 25‰; the... Tipo: Text Palavras-chave: Bivalve; Herpesviridae; Infection; Disease transmission; Risk analysis. Ano: 2016 URL: http://archimer.ifremer.fr/doc/00350/46100/45781.pdf A flow-through rearing system for ecophysiological studies of Pacific oyster Crassostrea gigas larvae Provedor de dados: 5 Autores: Rico Villa, Benjamin; Woerther, Patrice; Mingant, Christian; Le Piver, David; Pouvreau, Stephane; Hamon, Michel; Robert, Rene. While literature is relatively abundant on adult shellfish, ecophysiological studies at the larval stage are scarce because of both technical difficulties and inadequate methodology. A tool dedicated to provide basic information for larval ecophysiology was accordingly developed. Two steps were followed: first a flow-through method of Crassostrea gigas larval culture was perfected during a set of experiments in which rearing systems and larval density were assessed. Then a continuous hydrobiological data recorder was adapted with modifications to comply with our experimental aim. SILO (Système d'Instrumentation des Larves en flux Ouvert) allowed the successive acquisition of hydrobiological parameters in ten 150 l larval tanks in which larvae were reared... Tipo: Text Palavras-chave: Temperature; Ecophysiology; Bivalve larvae; Crassostrea gigas; Flow through. Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4167.pdf Adaptation du métabolisme respiratoire de l'huître creuse Crassostrea gigas Provedor de dados: 5 Autores: Le Moullac, Gilles. Summer mortality of the oyster result from the interaction of many factors. A respiratory assumption proposes a link between the supposed causes of the energy state, the cultivation methods and the medium. This work is focused towards the search for specific metabolic markers of environmental stresses. The cDNA of genes coding for PK and PEPCK were cloned and sequenced in order to detect their regulation level. The sediment nearness affects the state of energy reserves, the metabolic pathways of energy production implying a regulation of the PK. Face to the dissolved oxygen decline, oysters were oxyregulator but with a low regulation ability. In hypoxia, glycolysis was slowed down. PK activity was inhibited resulting from an increase in alanine sensitivity... Tipo: Text Palavras-chave: Régulation; Enzyme; Expression génique; Anoxie; Bivalve. Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/these-4315.pdf Production of microalgal concentrates by flocculation and their assessment as aquaculture feeds Provedor de dados: 5 Autores: Knuckey, R; Brown, M; Robert, Rene; Frampton, D. A novel technique was developed for the flocculation of marine microalgae commonly used in aquaculture. The process entailed an adjustment of pH of culture to between 10 and 10.6 using NaOH, followed by addition of a non-ionic polymer Magnafloc LT-25 to a final concentration of 0.5 mg L&#8722;1. The ensuing flocculate was harvested, and neutralised giving a final concentration factor of between 200- and 800-fold. This process was successfully applied to harvest cells of Chaetoceros calcitrans, C. muelleri, Thalassiosira pseudonana, Attheya septentrionalis, Nitzschia closterium, Skeletonema sp., Tetraselmis suecica and Rhodomonas salina, with efficiencies &#8805;80%. The process was rapid, simple and inexpensive, and relatively cost neutral with... Tipo: Text Palavras-chave: Chaetoceros; Thalassiosira; Flocculation; Concentrate; Bivalve; Algal paste. Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1820.pdf Evidence for interspecies transmission of oyster herpesvirus in marine bivalves Provedor de dados: 5 Autores: Arzul, Isabelle; Renault, Tristan; Lipart, Cécile; Davison, Andrew. Since 1991, numerous herpesvirus infections associated with high mortality have been reported around the world in various marine bivalve species. In order to determine whether these infections are due to ostreid herpesvirus-l (OsHV1), a previously characterized pathogen of the Japanese oyster (Crassostrea gigas), PCR analysis was carried out on 30 samples of larvae collected from four bivalve species (C. gigas, Ostrea edulis, Ruditapes decussatus and Ruditapes philippinarum), most exhibiting mortality prior to collection. All samples were shown to be infected by OsHV1. Viral genomes in three samples of C. gigas and three of R. philippinarum that originated from the same hatchery were unusual in bearing a deletion of at least 2.8 kbp in an inverted repeat... Tipo: Text Palavras-chave: Bivalve; Crassostrea gigas; Herpesvirus; Japanese oyster; Transmission. Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-705.pdf First evidence of a potential antibacterial activity involving a laccase-type enzyme of the phenoloxidase system in Pacific oyster Crassostrea gigas haemocytes Provedor de dados: 5 Autores: Luna-acosta, Andrea; Saulnier, Denis; Pommier, Mylene; Haffner, Philippe; De Decker, Sophie; Renault, Tristan; Thomas-guyon, Helene. Phenoloxidases (POs) are a group of copper proteins including tyrosinase, catecholase and laccase. In several insects and crustaceans, antibacterial substances are produced through the PO cascade, participating in the direct killing of invading microorganisms. However, although POs are widely recognised as an integral part of the invertebrate immune defence system, experimental evidence is lacking that these properties are conserved in molluscs, and more particularly in the Pacific oyster Crassostrea gigas. In the present study, Vibrio splendidus LGP32 and Vibrio aestuarianus 02/041 growths were affected, after being treated with C. gigas haemocyte lysate supernatant (HLS), and either a common substrate of POs, l-3,4-dihydroxyphenylalanine (l-DOPA), to... Tipo: Text Palavras-chave: Phenoloxidases; Antibacterial activity; Vibrio; Bivalve; Immunity. Ano: 2011 URL: http://archimer.ifremer.fr/doc/00045/15598/13001.pdf Etude comparative de différents indices de condition chez l'huître creuse (Crassostrea gigas) Provedor de dados: 5 Autores: Bodoy, Alain; Prou, Jean; Berthome, Jean-paul. Among the different condition indices, some of them were compared on a population of the japanese oyster, Crassostrea gigas. The follow-up of experimental racks was performed monthly during four years. The use of dry weight in condition indices gave a more precise and more reliable representation for the condition of the oysters than the fresh weight. The sensitivity of these indices, which corresponds to their ability in revealing small changes in the physiological condition, was calculated for each indice. Those exhibiting the best sensitivity were ranked as follow: dry weight on the difference between total weight and shell weight, dry weight on intervalve volume. Other indices including the ratio between dry weight and various assessments of the volume... Tipo: Text Palavras-chave: Bivalve; Crassostrea gigas; Indice de qualité; Indice de condition; Crassostrea gigas; Bivalve; Condition factor; Condition index. Ano: 1986 URL: http://archimer.ifremer.fr/doc/00000/2961/9155.pdf Polyploidy in the Manila clam Ruditapes philippinarum. II. Chemical induction of tetraploid embryos Provedor de dados: 5 Autores: Diter, Alain; Dufy, Christian. Tetraploidy was induced in embryos of Ruditapes philippinarum by treating the eggs with cytochalasin B. (CB). Two treatment times were found to be effective in inducing tetraplidy : 0 to 10 minutes after insemination (before formation of the first polar body) and 45 minutes after insemination (just before the first cleavage). Treatments, started 5 and 45 minutes after insemination, produced 64.4 ± 7.8% and 28.3 ± 2.3% tetraploid embrios, respectively, after 6 hours (on the average over 3 experiments). However, no tetraploids were detected in 4-month-old spat. Sperm entry delayed by the CB could be involved in induction of tetraploidy by early treatment. Tipo: Text Palavras-chave: Ruditapes philippinarum; Bivalve; Tetraploidy; Cytochalasin B.; Ruditapes philippinarum; Bivalve; Tétraploïdie; Cytochalasine B. Ano: 1990 URL: http://archimer.ifremer.fr/doc/1990/publication-2753.pdf Temporal variation of currents, particulate flux and organism supply at two deep-sea hydrothermal fields of the Azores Triple Junction Provedor de dados: 5 Autores: Khripounoff, Alexis; Vangriesheim, Annick; Crassous, Philippe; Segonzac, Michel; Lafon, V; Waren, A. In order to provide information on the production and the distribution of hydrothermal particulate and biological material produced by deep-sea hydrothermal vents, three moorings with sediment traps and currentmeters were deployed for one year in the Azores Triple Junction region. Two moorings were positioned close to two hydrothermal vent fields at the Mid-Atlantic Ridge (Rainbow, 36 degrees 14'N, 33 degrees 54'W, 2250 in depth and Lucky Strike 37 degrees 17'N, 32 degrees 17'W, 1600 in depth). A third was moored out of the influence of the Rainbow vents representing the reference of the pelagic flux in this area. At the Rainbow field, particulate flux (range 341-634 g m(-2)d(-1)) showed a high temporal variation controlled essentially by the current... Tipo: Text Palavras-chave: Bivalve reproduction; Bivalve larvae; Particle composition; Particle flux; Currents; Deep sea hydrothermal vents. Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4059.pdf Etat des connaissances sur la pathologie chez les mollusques bivalves Provedor de dados: 5 Autores: Fouche, Dominique. A short description of the disease causing organisms affecting bivalves is presented in this document, including the diagnosis, the oustanding research and bivalve husbandry. Mollusc populations may be safe from disease epizootics if an early prevention of disease is coupled with a strict regulatory system on transfer (according to what happens in agriculture). The species to culture may be thoroughly selected and risky populations moved. Imported stocks are strictly controlled. The researchcs's target is to improve the diagnosis tools and try to find resistant mollusc strains. Epidemiological surveys are also necessary to control diseases. Tipo: Text Palavras-chave: Transfer; Husbandry; Prophylatics; Bivalve; Protozoan; Virus; Bacteria; Metazoan; Transfert; Zootechnie; Prophylaxie; Bivalve; Protozoaire; Virus; Bactérie; Métazoaire. Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/rapport-1923.pdf Bilan énergétique chez les mollusques bivalves : terminologie et méthodologie - Compte rendu du groupe de travail réuni au laboratoire national "Ecosystème conchylicole" de l'IFREMER La Tremblade (13 et 14 septembre 1984) Provedor de dados: 5 Autores: Heral, Maurice; Lucas, Albert; Deslous Paoli, Jean-marc; Goulletquer, Philippe; Masse, Henri; LABEIM (Laboratoire de Biologie et d'Ecologie des Invertébrés Marins). En septembre 1984, MM. HERAL et DESLOUS-PAOLI organisèrent à La Tremblade, une "réunion méthodologie expérimentale mollusques". Cette rencontre faisait suite à un bref état qui s'était engagé au Congrès de Montpellier sur les "Bases biologiques de l'Aquaculture", où il était apparu que la terminologie et la méthodologie concernant le métabolisme des bivalves devaient être clarifiées. Grâce à une large information auprès des spécialistes français de la physiologie des bivalves, la réunion à La Tremblade a réuni un grand nombre de participants très motivés. Pendant 2 jours des discussions très animées ont permis de bien comprendre les échanges entre les bivalves et leur environnement et d'y jeter un regard neuf, ce qui impliquait une analyse critique du... Tipo: Text Palavras-chave: Terminologie; Méthodologie; Bilan d'énergie; Physiologie; Bivalve; Terminology; Methodology; Energy budget; Physiology; Bivalve molluscs. Ano: 1987 URL: http://archimer.ifremer.fr/doc/00014/12562/9432.pdf Morphology and ecology of bivalve molluscs from Admiralty Bay, King George Island, Antarctica Provedor de dados: 52 Autores: Absher,Theresinha M.; Feijó,Angela R. Bivalve species were collected from shallow coastal areas of King George Island (Martel, Mackellar and Ezcurra Inlets of Admiralty Bay). Twenty one species belonging to 16 genera and 12 families were identified and their morphometric and morphological shell characteristics were described. Three main characteristics were found to be common to the majority of the bivalve species sampled: 1) thin fragile shells; 2) small size of individuals (76%), and 3) the lack of true cardinal teeth (72%). Comparison of calcium data from a tropical estuary and a subantarctic coastal shallow area suggested that the calcium in the sea water was not a constraint to shell building but shell thickness could be an adaptation to the efficiency of energy partitioning. Small... Tipo: Journal article Palavras-chave: Bivalve species; Antarctic bivalves; King George Island; Antarctica. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000400008 Molecular cloning and seasonal expression of oyster glycogen phosphorylase and glycogen synthase genes Provedor de dados: 5 Autores: Bacca, Helene; Huvet, Arnaud; Fabioux, Caroline; Daniel, Jean-yves; Delaporte, Maryse; Pouvreau, Stephane; Van Wormhoudt, A; Moal, Jeanne. To investigate the control at the mRNA level of glycogen metabolism in the cupped oyster Crassostrea gigas, we report in the present paper the cloning and characterization of glycogen phosphorylase and synthase cDNAs (Cg-GPH and Cg-GYS, respectively, transcripts of main enzymes for glycogen use and storage), and their first expression profiles depending on oyster tissues and seasons. A strong expression of both genes was observed in the labial palps and the gonad in accordance with specific cells located in both tissues and ability to store glucose. Cg-GPH expression was also found mainly in muscle suggesting ability to use glycogen as readily available glucose to. supply its activity. For seasonal examinations, expression of Cg-GYS and Cg-GPH genes... Tipo: Text Palavras-chave: Reproduction; Regulation; Oyster; Glycogen; Expression; Gene; Energy; Crassostrea gigas; Bivalve. Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-435.pdf Molecular identification of Vibrio tapetis, the causative agent of the brown ring disease of Ruditapes philippinarum Provedor de dados: 5 Autores: Paillard, Christine; Gausson, Sabrina; Nicolas, Jean-louis; Le Pennec, Jean Paul; Haras, Dominique. Vibrio tapetis is the marine bacterium responsible for the brown ring disease (BRD) affecting die manila clam, Ruditapes philippinarum. Identification of V tapelis has been previously performed using biochemical criteria and serological procedures. All of these methods are time consuming and ill-adapted to individual screening. This study describes an oligonucleotidic probe (Vt446) and two PCR primers, deduced from the 16S rDNA sequence, allowing a fast and specific V tapetis identification using dot blot hybridisation and species-specific primed PCR (SSP-PCR). The probe and primers have been tested on 60 strains, including referenced Vibrio sp., Gram negative and positive bacteria, marine bacteria samples and isolated clam bacteria. For all the 19 V... Tipo: Text Palavras-chave: DOT BLOT hybridisation; SSP PCR; Vibriosis; Bivalve; Detection; Diagnostic. Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1692.pdf A Dynamic Energy Budget (DEB) growth model for Pacific oyster larvae, Crassostrea gigas Provedor de dados: 5 Autores: Rico-villa, Benjamin; Bernard, Ismael; Robert, Rene; Pouvreau, Stephane. Tipo: Text Palavras-chave: Dynamic Energy Budget; Crassostrea gigas; Bivalve larvae; Ecophysiology; Growth. Ano: 2010 URL: http://archimer.ifremer.fr/doc/00009/11996/9040.pdf Transmission expérimentale du virus de type herpes chez l'huître creuse, Crassostrea gigas Provedor de dados: 5 Autores: Arzul, Isabelle. Au cours de l'été 1991, d'importantes mortalités de larves d'huître creuse, Crassostrea gigas, ont été observées dans des écloseries, simultanément en France (Comps et Cochennec, 1993) et en Nouvelle-Zélande (Hine & al., 1992). Les examens histologiques ainsi que des examens réalisés en microscopie électronique à transmission d'échantillons de larves présentant des mortalités ont permis de détecter la présence de particules virales associées à des lésions cellulaires. D'après la morphologie, la taille, les localisations intranucléaires et cytoplasmiques du virus, il est possible d'envisager son appartenance à la famille des Herpesviridae. De nouvelles mortalités sporadiques ont été observées au cours des étés suivants sur des lots de larves élevées... Tipo: Text Palavras-chave: Virus; Herpesviridae; Bivalve; Crassostrea gigas; Etiologie; Epidémiologie; Diagnostic; Reproduction infection. Ano: 1998 URL: http://archimer.ifremer.fr/doc/00106/21711/19289.pdf Why carrying capacity models are useful tools for management of bivalve molluscs culture Provedor de dados: 5 Autores: Heral, Maurice. In areas where oyster or mussel culture is very intensive, declines of growth rate and decreases of survival rate have occurred. For these reasons, plans have been proposed to regulate the cultivated biomasses in order to fit the carrying capacity of the different ecosystems (Heral et al 1990; Heral, 1991). In areas where new aquaculture of molluscs is beginning, oyster or mussel farmers need to know how large the extension of the culture could be and what the maximal densities should be in order to obtain the maximum economic benefit. Furthermore, as mollusc cultures are developed in coastal areas, they are very susceptible to changes in environmental conditions that can modify trophic relationships, or directly reduce growth rate, physiological... Tipo: Text Palavras-chave: Oyster model; Analytical model; Bivalve molluscs culture; Models. Ano: 1993 URL: http://archimer.ifremer.fr/doc/1993/publication-6104.PDF Characterization of a gonad-specific transforming growth factor-beta superfamily member differentially expressed during the reproductive cycle of the oyster Crassostrea gigas Provedor de dados: 5 Autores: Fleury, Elodie; Fabioux, Caroline; Lelong, C; Favrel, P; Huvet, Arnaud. Through differential screening between oyster families selected for high and low summer survival, we have characterized a new transforming growth factor-beta (TGF-beta) superfamily member. This novel factor, named oyster-gonadal-TGF beta-like (og-TGF beta-like), is synthesized as a 307 amino acid precursor and displays 6 of the 7 characteristic cysteine residues of the C-terminal, mature peptide. Sequence comparison revealed that og-TGF beta-like has a low percentage of identity with other known TGF-beta superfamily members, suggesting that og-TGF beta-like is a derived member of this large superfamily. Real-time PCR (RT-PCR) analysis in different oyster tissues showed that og-TGF beta-like is specifically expressed in both male and female gonads, at... Tipo: Text Palavras-chave: Reproduction; Oyster; Gene expression; Transforming growth factor; Bivalve mollusc. Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3899.pdf Bacterial disease in marine bivalves, a review of recent studies: Trends and evolution Provedor de dados: 5 Autores: Paillard, Christine; Leroux, Frederique; Borrego, Juan. The main microbial diseases affecting marine cultured bivalves have been revised on the basis of the etiologic agents, pathogenesis and pathogenicity. Several recent bivalve-interaction models have been studied, including Pecten larvae-Vibrio pectinicida, brown ring disease, juvenile oyster disease, Pacific oyster nocardiosis and summer mortalities of oysters. In addition, the taxonomy and phylogeny of new potential bivalve pathogens and their virulence factors have been established. Facing the difficulty of identifying bacterial strains associated with molluscan diseases (mainly vibriosis), polyphasic approaches have been developed to correlate the phenotype and genotype of potential pathogens. By evaluating likely virulence mechanisms, developing... Tipo: Text Palavras-chave: Diagnostic tools; Virulence factors; Pathogenicity; Pathogenesis; Etiology; Bivalve mollusc; Vibriosis. Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-407.pdf A microarray-based analysis of oocyte quality in the European clam Ruditapes decussatus Provedor de dados: 5 Autores: De Sousa, Joana Teixeira; Milan, Massimo; Pauletto, Marianna; Bargelloni, Luca; Joaquim, Sandra; Matias, Domitilia; Matias, Ana Margarete; Quillien, Virgile; Leitao, Alexandra; Huvet, Arnaud. A microarray-based analysis was performed with the objective of describing genomic features of oocytes and looking for potential markers of oocyte quality in the economically important European clam, Ruditapes decussatus. Oocytes of 25 females from Ria de Aveiro, Western coast of Portugal (40°42′N; 08°40′W) were selected for this study and oocyte quality was estimated by success of D-larval rate under controlled conditions, which appeared to vary from 0 to 95 %. By genome-wide expression profiling with a DNA microarray, 526 probes appeared differentially expressed between two groups representing the largest and smallest value of D larval rates, named good (represented by a mean D-larval yield of 57 ± 22%) and poor (9 ± 5%) quality oocytes. Enrichment... Tipo: Text Palavras-chave: Ruditapes decussatus; Oocyte; Developmental success; Gene expression; Marine bivalve; Transcriptomics. Ano: 2015 URL: http://archimer.ifremer.fr/doc/00260/37147/35676.pdf Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment Provedor de dados: 5 Autores: Morvezen, Romain; Cornette, Florence; Charrier, Gregory; Guinand, Bruno; Lapegue, Sylvie; Boudry, Pierre; Laroche, Jean. We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops. Tipo: Text Palavras-chave: Microsatellites; Polymerase chain reaction; Parentage; Assignment; Bivalve mollusc. Ano: 2013 URL: http://archimer.ifremer.fr/doc/00137/24822/22907.pdf Reduction of adhesion properties of Ruditapes philippinarum hemocytes exposed to Vibrio tapetis Provedor de dados: 5 Autores: Choquet, Gwenaelle; Soudant, P; Lambert, C; Nicolas, Jean-louis; Paillard, C. Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of other Vibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V tapetis compared to that of V splendidus and V pectenicida suggests a specific pathogenicity... Tipo: Text Palavras-chave: Cytotoxicity; Flow cytometry; Vibrio tapetis; Ruditapes philippinarum; Bivalve hemocytes; Pathogenicity. Ano: 2003 URL: http://archimer.ifremer.fr/doc/00000/10761/7590.pdf Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat Provedor de dados: 5 Autores: Luna-acosta, Andrea; Renault, Tristan; Thomas-guyon, Helene; Faury, Nicole; Saulnier, Denis; Budzinski, Helene; Le Menach, K.; Pardon, P.; Fruitier-arnaudin, I.; Bustamante, Paco. In the context of massive summer mortality events of the Pacific oyster Crassostrea gigas, the aim of this study was to investigate the early effects on genes, enzymes and haemocyte parameters implicated in immune defence mechanisms in C. gigas oysters exposed to a potentially hostile environment, i.e. to an herbicide alone or within a mixture. Following 2 h of exposure to the herbicide diuron at 1 mu g L-1 the repression of different genes implicated in immune defence mechanisms in the haemocytes and the inhibition of enzyme activities, such as laccase-type phenoloxidase (PO) in the plasma, were observed. The inhibition of superoxide dismutase (SOD) activity in the plasma was also observed after 6 and 24 h of exposure. In the mixture with the herbicides... Tipo: Text Palavras-chave: Bivalve; Pollution; Gene expression; Laccase; Superoxide dismutase; Phagocytosis. Ano: 2012 URL: http://archimer.ifremer.fr/doc/00083/19435/17475.pdf Temperature effect on survival, growth, and triacylglycerol content during the early ontogeny of Mytilus edulis and M. trossulus Provedor de dados: 5 Autores: Rayssac, Nathalie; Pernet, Fabrice; Lacasse, Olivia; Tremblay, Rejean. Temperature is a major factor contributing to the biogeography of intertidal poikilotherms. The population dynamics of intertidal invertebrates like mussels are strongly dependent on larval supply and larval mortality. Unlike adults, which are resistant to fluctuating environmental conditions, larvae are highly sensitive to the physicochemical conditions of the pelagic zone. The effects of rearing temperature on larval and post-larval performance of 2 coexisting mussels species, Mytilus edulis and M. trossulus, were examined in this study. In the first experiment, larvae of both species were reared separately at constant temperatures of 10, 17, and 24 degrees C from D-larvae to the dissoconch stage. In the second experiment, pediveligers were reared under... Tipo: Text Palavras-chave: Thermal adaptation; Bivalve mollusk; Selection; Biogeography; Lipid; Larval ecology. Ano: 2010 URL: http://archimer.ifremer.fr/doc/00019/13028/10159.pdf Reproductive effort of Pacific oysters: A trait associated with susceptibility to summer mortality Provedor de dados: 5 Autores: Huvet, Arnaud; Normand, Julien; Fleury, Elodie; Quillien, Virgile; Fabioux, Caroline; Boudry, Pierre. Summer mortality of the Pacific oyster Crassostrea gigas is the result of a complex interaction between oysters, their environment and their pathogens. The physiological status of an oyster, especially its reproductive status, is suspected to play a significant role in the outcome of this interaction. As genetic variability exists for susceptibility to summer mortality, resistant (R) and susceptible (S) oyster lines were produced using a divergent selection scheme. The present paper reports a histological study on gonad area, which is representative of reproductive effort, in randomly chosen five R and five S oyster lines. The R lines showed a significantly lower gonad area than the S lines (P<0.001). with an estimated mean difference of 12.5%, whereas,... Tipo: Text Palavras-chave: Bivalve mollusc; Crassostrea gigas; Reproduction; Selected lines; Summer mortality. Ano: 2010 URL: http://archimer.ifremer.fr/doc/00006/11766/8502.pdf Spatial distribution and nutritional requirements of the endosymbiont-bearing bivalve Loripes lacteus (sensu Poli, 1791) in a Mediterranean Nanozostera noltii (Hornemann) meadow Provedor de dados: 5 Autores: Rossi, Francesca; Colao, Elodie; Jose Martinez, Maria; Klein, Judith C.; Carcaillet, Frederique; Callier, Myriam; De Wit, Rutger; Caro, Audrey. Sulphur-oxidising endosymbiont-bearing bivalves often inhabit seagrass meadows, where they can control sulphide levels and variably contribute to carbon cycling, by feeding on endosymbiotic bacteria and/or on particulate organic matter from the water column. The patterns of variability in their feeding mode and their spatial distribution within the seagrass meadows are however poorly studied. Seagrass beds form naturally patchy habitats with seagrass-sand edges that may have variable effects on different organisms. The present study aims at understanding differences in feeding mode and abundance of the endosymbiont-bearing bivalve Loripes lacteus (sensu Poli, 1791) as well as the physiological conditions of its endosymbiotic populations between edge and... Tipo: Text Palavras-chave: Bivalve; Food web; Mediterranean; Seagrass; Stable isotopes; Symbiosis. Ano: 2013 URL: http://archimer.ifremer.fr/doc/00138/24911/26373.pdf Influence of food density and temperature on ingestion, growth and settlement of Pacific oyster larvae, Crassostrea gigas Provedor de dados: 5 Autores: Rico Villa, Benjamin; Pouvreau, Stephane; Robert, Rene. Ingestion, growth and metamorphosis of Pacific oyster, Crassostrea gigas, larvae were studied under controlled conditions of food density and temperature using a combination of a flow-through rearing system and a hydrobiological monitoring device. In a first experiment larvae were exposed to three different phytoplankton densities (12, 20 and 40 cells &#956;l-1) while in a second trial larvae were reared at five different temperatures (17, 22, 25, 27 and 32°C). Both food concentration and temperature significantly affected the larval physiology throughout the entire development from D-veliger to young spat. Larvae survived over a wide range of both environmental parameters with high survival at the end of experiments. The feeding functional response... Tipo: Text Palavras-chave: Temperature; Food; Growth; Ecophysiology; Bivalve larvae; Crassostrea gigas. Ano: 2009 URL: http://archimer.ifremer.fr/doc/2008/publication-4747.pdf Molecular characterization of two isoforms of defensin from hemocytes of the oyster Crassostrea gigas Provedor de dados: 5 Autores: Gonzalez, Marcelo; Gueguen, Yannick; Desserre, Geraldine; De Lorgeril, Julien; Romestand, Bernard; Bachere, Evelyne. Antimicrobial peptides (AMPs) are important components of the host innate immune response against microbial invasion. We previously characterized the first AMP from an oyster, a defensin, that was shown to be continuously expressed in the mantle of Crassostrea gigas. In this study, we report the cDNA cloning of two new isoforms of the defensin AMP family (Cg-defh1 and Cg-defh2) from the hemocytes of the oyster. The deduced amino acid sequences reveal two peptides of 73 amino acid residues with a mature portion consisting of 43 amino acid residues. Cy-Defh1 and CyDefh2 share 86% amino acid identity and belong to the "arthropod-molluscs defensin family". qRT-PCR analyses indicate that Cg-defh2 is continuously expressed in the hemocytes of C. gigas. In... Tipo: Text Palavras-chave: Defensin; Antimicrobial peptide; Hemocytes; Pacific oyster; Marine invertebrate; Mollusc bivalve. Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2331.pdf Influence of low temperatures on the survival of the Pacific oyster (Crassostrea gigas) infected with Ostreid herpesvirus type 1 Provedor de dados: 5 Autores: Pernet, Fabrice; Tamayo, David; Petton, Bruno. Mortalities of oyster seed of Crassostrea gigas associated with ostreid herpes virus OsHV-1 μVar have been observed in many oyster producing countries since 2008. The objective of this study was to further investigate whether low temperature treatments can offer a viable option to mitigate oyster mortalities. An experiment was set-up to further evaluate the effect of low temperature treatments (10 and 13°C vs. 21°C) and their duration (6 d to 83 d) on the survival of oysters previously infected with OsHV-1 μVar by means of exposure to field conditions in areas where mortalities were occurring. Average survival of oysters infected with OsHV-1μVar was 71% after 83 d at low temperatures compared to only 23% in controls maintained at 21°C. During... Tipo: Text Palavras-chave: Bivalve; Disease; OsHV-1; Oyster; Temperature; Virus. Ano: 2015 URL: http://archimer.ifremer.fr/doc/00259/37047/35555.pdf Effect of sampling location, release technique and time after activation on the movement characteristics of scallop (Pecten maximus) sperm Provedor de dados: 5 Autores: Suquet, Marc; Quere, Claudie; Mingant, Christian; Lebrun, Luc; Ratiskol, Dominique; Miner, Philippe; Cosson, Jacky. Sperm characteristics of scallops have not been well described in the scientific literature. The effects of sperm release technique (thermal shock versus serotonin injection), of sperm collection technique (testis sampling versus serotonin injection), of sperm sampling location along the genital tract, of in vitro sperm maturation, and of time post activation on scallop sperm characteristics were assessed in the present work. Whatever sperm release technique used, no significant differences were observed regarding the percentage of motile spermatozoa and the velocity of the average path (YAP). Compared to testicular sperm, a higher percentage of motile spermatozoa, YAP and intracellular adenosine triphosphate (ATP) content were observed for sperm shed... Tipo: Text Palavras-chave: Spermatozoa; CASA; Maturation; Sperm motility; Bivalve; Pecten maximus. Ano: 2013 URL: http://archimer.ifremer.fr/doc/00157/26852/24983.pdf Overwiew on selective breeding and genetic improvement in bivalve shellfish Provedor de dados: 5 Autores: Boudry, Pierre. Dr. Boudry focussed his talk on oysters, which are the most important bivalve species produced world wide (more than 4 million tons). One of the main characteristic of bivalve aquaculture is that most of the juveniles ("seed or "spat") are collected from natural recruitment (e.g. 100% of the European mussel production). Hatchery propagation is used in the case of introduced species (e.g. Crassostrea gigas in USA) or limited natural recruitment. The most significant genetic improvement for the production of Pacific oyster (C. gigas) to date has been obtained through the breeding of triploids, especially since the development of tetraploids. Triploidy lead to highly reduced gametogenesis and, as a result better growth and survival. Many quantitative... Tipo: Text Palavras-chave: Crassostrea gigas; Oyster; Genetic variability; Bivalve shellfish; Selective breeding; Genetic. Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3479.pdf Pacific oyster (Crassostrea gigas) hemocyte are not affected by a mixture of pesticides in short-term in vitro assays Provedor de dados: 5 Autores: Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan. Pesticides are frequently detected in estuaries among the pollutants found in estuarine and coastal areas and may have major ecological consequences. They could endanger organism growth, reproduction or survival. In the context of high mortality outbreaks affecting Pacific oysters, Crassostrea gigas, in France since 2008, it appears of importance to determine the putative effects of pesticides on oyster susceptibility to infectious agents. Massive mortality outbreaks reported in this species, mainly in spring and summer, may suggest an important role played by the seasonal use of pesticides and freshwater input in estuarine areas where oyster farms are frequently located. To understand the impact of some pesticides detected in French waters, their effects... Tipo: Text Palavras-chave: Immunity; Hemocytes; Pesticides; Pacific oyster; Crassostrea gigas; Flow cytometry; Bivalve. Ano: 2014 URL: http://archimer.ifremer.fr/doc/00144/25555/23700.pdf Modeling the impact of oyster culture on a mudflat food web in Marennes-Oleron Bay (France) Provedor de dados: 5 Autores: Leguerrier, D; Niquil, Nathalie; Petiau, A; Bodoy, Alain. We used a carbon-based food web model to investigate the effects of oyster cultivation on the ecosystem of an intertidal mudflat. A previously published food web model of a mudflat in Marennes-Oleron Bay, France, was updated with revised parameters, and a realistic surface area and density of existing oyster cultures on the mudflat. We developed 2 hypothetical scenarios to estimate the impact of oyster cultivation on the food web structure of the ecosystem: one with no oysters, the other with a doubled area devoted to cultivated oysters in the bay. Oysters are direct trophic competitors of other filter feeders, and their presence modifies benthic-pelagic coupling by forcing a shift from pelagic consumers to benthic consumers. Increasing the surface area of... Tipo: Text Palavras-chave: Carbon flux; Inverse analysis; Food web; Network analysis; Bivalve culture. Ano: 2004 URL: http://archimer.ifremer.fr/doc/00000/10816/7583.pdf A high load of non-neutral amino-acid polymorphisms explains high protein diversity despite moderate effective population size in a marine bivalve with sweepstakes reproduction Provedor de dados: 5 Autores: Harrang, Estelle; Lapegue, Sylvie; Morga, Benjamin; Bierne, Nicolas. Marine bivalves show among the greatest allozyme diversity ever reported in Eukaryotes, putting them historically at the heart of the neutralist-selectionist controversy on the maintenance of genetic variation. Although it is now acknowledged that this high diversity is most probably a simple consequence of a large population size, convincing support for this explanation would require a rigorous assessment of the silent nucleotide diversity in natural populations of marine bivalves, which has not yet been done. This study investigated DNA sequence polymorphism in a set of 37 nuclear loci in wild samples of the flat oyster Ostrea edulis. Silent diversity was found to be only moderate (0.7%), and there was no departure from demographic equilibrium under the... Tipo: Text Palavras-chave: Nucleotide; Polymorphism; Marine bivalve; Deleterious; Mutations; Genetic load; Ostrea edulis. Ano: 2013 URL: http://archimer.ifremer.fr/doc/00125/23610/21444.pdf Etude expérimentale de différentes conditions de mileu sur le comportement du parasite de la glande digestive de l'huître plate (Ostrea edulis L.) Provedor de dados: 5 Autores: Comps, Michel. During trials on the influence of environmental factors on the parasite of the flat oyster digestive gland, we observed that some experimental conditions including the re-immersion after a several day-exsiccation period could result in a decrease of the parasitism rate. Tipo: Text Palavras-chave: Glande digestive; Huître plate; Ostrea edulis; Bivalve; Pathologie; Parasitologie. Ano: 1975 URL: http://archimer.ifremer.fr/doc/1975/acte-6168.PDF Report of the workshop on shellfish bivalve cultivation, growth, modelling and impact on the ecosystem Provedor de dados: 5 Autores: Heral, Maurice; Bayne, B. The Workshop was held in Plymouth (6-10 October 1996) in the Royal Plymouth Corinthian Yacht club organised by the Plymouth Marine Laboratory under the joint charmanships of B. Bayne et M. Héral. 36 scientists were participated to this meeting from 11 countries, 8 belonging to ICES (Annex 1) 30 communications have been presented. This meeting was supported by an European Union contrat from DG14 with a concerted action AIR3 CT 942219 on Trophic capacity of coastal zone of oysters, mussels and cockles cultivation. This contract has paid the travel cost of all the participants. Tipo: Text Palavras-chave: Coastal systems; Carrying capacity; Physiological results; Oyster; Shefflish bivalve. Ano: 1996 URL: http://archimer.ifremer.fr/doc/1996/acte-7350.pdf Cellular and molecular hemocyte responses of the Pacific oyster, Crassostrea gigas, following bacterial infection with Vibrio aestuarianus strain 01/32 Provedor de dados: 5 Autores: Labreuche, Yannick; Lambert, Christophe; Soudant, Philippe; Boulo, Viviane; Huvet, Arnaud; Nicolas, Jean-louis. The strategies used by bacterial pathogens to circumvent host defense mechanisms remain largely undefined in bivalve molluscs. In this study, we investigated experimentally the interactions between the Pacific oyster (Crassostrea gigas) immune system and Vibrio aestuarianus strain 01/32, a pathogenic bacterium originally isolated from moribund oysters. First, an antibiotic-resistant V. aestuarianus strain was used to demonstrate that only a limited number of bacterial cells was detected in the host circulatory system, suggesting that the bacteria may localize in some organs. Second, we examined the host defense responses to V. aestuarianus at the cellular and molecular levels, using flow-cytometry and real-time PCR techniques. We showed that hemocyte... Tipo: Text Palavras-chave: Crassostrea gigas; Oyster; Pathogenesis; Vibrio aestuarianus; Bivalve immunity. Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2182.pdf Marine diatoms sustain growth of bivalves in a Mediterranean lagoon Provedor de dados: 5 Autores: Pernet, Fabrice; Malet, Nathalie; Pastoureaud, Annie; Vaquer, Andre; Quere, Claudie; Dubroca, Laurent. Carbon stable isotopes and fatty acids were measured in the suspended particulate organic matter (POM) of the Thau lagoon to study its qualitative temporal changes in relation to environmental factors and to identify the food sources of bivalves over a one-yr-cycle in relation to their growth. Reciprocally, the impact of shellfish farming on POM was also studied. Oysters and mussels were sampled and measured for biometry, stable isotopes and fatty acid composition. Water samples were collected at two sites, both inside and outside of the shellfish farming area, to determine concentrations in POM, chlorophyll a (Chl a) and stable isotopes. Carbon isotopes and fatty acids in bivalves reflected seasonal changes in food sources, which varied consistently with... Tipo: Text Palavras-chave: Aquaculture; Bivalve; Competition; Feeding ecology; Growth; Trophic markers. Ano: 2012 URL: http://archimer.ifremer.fr/doc/00075/18575/16177.pdf Image analysis : a new method for estimating triploidy in commercial bivalves Provedor de dados: 5 Autores: Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Noiret, Christophe; Peudenier, Isabelle; Grizel, Henri. For determination of ploidy levels in bivalves, three techniques are now routinely used: karyological determination. microfluorimetry and flow cytometry. This paper proposes an alternative technique which is based on determination of the optical density (OD) of stained nuclei using image analysis. A karyological and image analysis comparative experiment conducted on juveniles of Crassostrea gigas (Thunberg) and Ruditapes philippinarum (Adam & Reeve) is described and highlights the advantages of the image analysis technique. Initially developed on C. gigas and R. philippinarum, the image analysis method was easily extended to other bivalve species and to different developmental stages, as demonstrated by two experiments conducted on Ostrea edulis (L.).... Tipo: Text Palavras-chave: Estimation bivalve triploidy; Image analysis; Triploid; Genetic; Crassostrea gigas; Oysters. Ano: 1994 URL: http://archimer.ifremer.fr/doc/1994/publication-3200.pdf DEB2009 Symposium : Dynamic Energy Budget, 30 years of research for metabolic organization, Book of abstracts Provedor de dados: 5 We are pleased to welcome you to at the first Symposium on Metabolic organization in Brest, 19-22 April, 2009. This conference is being organized jointly by Ifremer (French Institute for the Research and Exploitation of the Sea), the Department of Theoretical Biology at Vrije Universiteit Amsterdam, the Universities of Brest (UBO), Marseille (LMGEM-UMR CNRS 6117), Caen (UMRPE2M, LBBM) and Lisbon (Instituto Superior Técnico), the IRD (French Research Institute for Development), the INRA (French National Institute for Agricultural Research), the NIOZ (RoyalNetherlands Institute for Sea Research), the IMARES Wageningen (Institute for Marine Resources and Ecosystem Studies), as all these research teams are involved in research on DEB theory. You are 81 persons... Tipo: Text Palavras-chave: Ecology; Bivalve; Chemistry; Ecotoxicology; Physiology; Dynamic energy budget; Biology. Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/acte-6790.pdf Toxicity assessment of peptaibols and contaminated sediments on Crassostrea gigas embryos Provedor de dados: 5 Autores: Poirier, Laurence; Quiniou, Francoise; Ruiz, Nicolas; Montagu, Monique; Amiard, Jean-claude; Pouchus, Yves François. Peptaibols are known membrane-modifying peptides that were recently detected in marine sediments and mussels collected from a shellfish farming area (Fier d'Ars, Atlantic coast, France). In this investigation, embryotoxicity bioassays with oysters (Crassostrea gigas) were performed to assess acute toxicity of alamethicin and different groups of peptaibols produced by a Trichoderma longibrachiatum strain isolated from marine environment. C gigas embryos appeared very sensitive to all the metabolites examined with higher toxic effects for long-sequence peptides (EC50 ranging from 10 to 64nM). D-shaped larvae with mantle abnormality were particularly noticed when peptaibol concentrations increased. Disturbances of embryogenesis were also observed following... Tipo: Text Palavras-chave: Risk assessment; Embryotoxicity; Bivalve bioassay; Mycotoxins; Marine fungi. Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2680.pdf Evidence in oyster of a plasma extracellular superoxide dismutase which binds LPS Provedor de dados: 5 Autores: Gonzalez, Marcelo; Romestand, Bernard; Fievet, Julie; Huvet, Arnaud; Lebart, M; Gueguen, Yannick; Bachere, Evelyne. We have characterized in the oyster Crassostrea gigas an extracellular superoxide dismutase (Cg-EcSOD) which appears to bind lipopolysaccharides (LPS). The protein has been purified from the oyster plasma and identified as a Cu/ZnSOD according to its N-terminal sequencing and biological activity. Cg-EcSOD expression and synthesis are restricted to hemocytes as revealed by in situ hybridization and immunocytochemistry. Cg-EcSOD-expressing hemocytes were seen in blood circulation, in connective tissues, and closely associated to endothelium blood vessels. Cg-EcSOD presents in its amino acid sequence a LPS-binding motif found in the endotoxin receptor CD14 and we show that the protein displays an affinity to Escherichia coli bacteria and with LPS and Lipid A.... Tipo: Text Palavras-chave: Beta integrin; Oxidative burst; Hemocyte; Crassostrea gigas; Invertebrate; Mollusc bivalve. Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-3583.pdf Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas Provedor de dados: 5 Autores: Bigot, Laetitia; Zatylny-gaudin, Celine; Rodet, Franck; Bernay, Benoit; Boudry, Pierre; Favrel, Pascal. Gonadotropin-releasing hormone (GnRH), a key neuropeptide regulating reproduction in vertebrates has now been characterized in a number of non-vertebrate species. Despite the demonstration of its ancestral origin, the structure and the function of this family of peptides remain poorly known in species as distant as lophotrochozoans. In this study, two GnRH-related peptides (Cg-GnRH-a and CgGnRH-G) were characterized by mass spectrometry from extracts of the visceral ganglia of the Pacific oyster Crassostrea gigas. These peptides showed a high degree of sequence identity with GnRHs of other mollusks and annelids and to a lesser extent with those of vertebrates or with AKH and corazonins of insects. Both the mature peptides and the transcript encoding the... Tipo: Text Palavras-chave: Mollusk; Bivalve; Oyster; Neuroendocrinology; Reproduction; GnRH. Ano: 2012 URL: http://archimer.ifremer.fr/doc/00079/19003/17477.pdf Anatomical study on Myoforceps aristatus, an invasive boring bivalve in S.E. Brazilian coast (Mytilidae) Provedor de dados: 84 Autores: Simone,Luiz Ricardo L.; Gonçalves,Eric Pedro. The bivalve Myoforceps aristatus (Dillwyn, 1817), also known as Lithophaga aristata, have been recently collected in the coasts of Rio de Janeiro and São Paulo, Brazil; a species that bores shells of other mollusks. This occurrence has been interpreted as an invasion of this species, originally from the Caribbean. The distinguishing character of the species is the posterior extensions of the shell crossing with each other. Because specimens with this character have also been collected in the Pacific Ocean, they all have been considered a single species. However, it is possible that more than one species may be involved in such worldwide distribution. With the objective of providing full information based on Atlantic specimens, a complete anatomical... Tipo: Journal article Palavras-chave: Myoforceps aristatus; Biological invasion; Boring bivalve; Brazil; Anatomy; Systematics. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0031-10492006000600001 Analysis of shell colour determinism in the Manila clam Ruditapes philippinarum Provedor de dados: 5 Autores: Peignon, Jean-marie; Gerard, Andre; Naciri, Yamama; Ledu, Christophe; Phelipot, Pascal. The Manila clam presents a large polymorphism for shell colours and patterns. No experiment has been conducted to study the determinism of such a polymorphism which has been well known for a long time. The aim of this study was firstly to determine the genetic variation for shell colour and secondly to produce some homozygous lines for the shell colouring. These lines could then be used as controls for laboratory experiments. The colouring is defined by three characters: asymmetry, ornamentation and hue. Clams with asymmetrical colouring are not fully pigmented, except for a posterior band on the left valve. Clams with symmetrical colouring are fully pigmented on the two valves. Four cross series were conducted between the different characters: Asymmetry... Tipo: Text Palavras-chave: Mollusque; Bivalve; Palourde; Ruditapes philippinarum; Génétique; Hérédité mendélienne; Coloration; Asymétrie; Mollusc; Bivalve; Manila clam; Ruditapes philippinarum; Genetics; Mendelian inheritance; Colouration; Asymmetry. Ano: 1995 URL: http://archimer.ifremer.fr/doc/1995/publication-3204.pdf Relations entre l’état d’une ressource et son exploitation via la compréhension et la formalisation des interactions de socio-écosystèmes. Application à la palourde japonaise (Venerupis philippinarum) du bassin d’Arcachon. Provedor de dados: 5 Autores: Caill-milly, Nathalie. Significant component of the marine benthic ecosystem, exploited bivalves are mainly located inside the coastal area, which temporal and spatial features generate natural population fluctuations and contribute to considerable biomass and demographic structure changes added to anthropogenic activities’ effects (fishing, pollution,…). Understanding of the relationships between the species, its environment and its exploitation is crucial and their formalization, including for management purposes, requires development of mathematical models that aim at describing the functioning of the system, to explain the observed phenomenon in terms of cause and effect or predict effects of new causes. Originating from venerid culture trials carried out in France in the... Tipo: Text Palavras-chave: Bivalve; Morphométrie; Allométrie; Dynamique des populations; Système dynamique; Gestion; Palourde japonaise; Venerupis philippinarum; Bivalve; Morphometry; Allometry; Population dynamics; Dynamic system; Management; Manila clam; Venerupis philippinarum. Ano: 2012 URL: http://archimer.ifremer.fr/doc/00139/25034/23143.pdf Effects of stocking-density in flow-through system on the mussel Perna perna larval survival Provedor de dados: 2 Autores: Turini, Caio Silva; Universidade Federal de Santa Catarina; Sühnel, Simone; Universidade Federal de Santa Catarina; Lagreze-Squella, Francisco José; Universidade Federal de Santa Catarina; Ferreira, Jaime Fernando; Universidade Federal de Santa Catarina; Melo, Claudio Manoel Rodrigues de; Universidade Federal de Santa Catarina. This study evaluated the effects of larval densities, cultured in flow-through system, in the yield and quantity of larvae. The first experiment compared the larval yield of a flow-through system (80 larvae mL-1) and a batch system (8 larvae mL-1), which resulted in a higher yield and number of larvae in the flow-through system, after 23 days. The second experiment compared two larval densities (20 and 45 larvae mL-1) in flow-through system. The densities affected the yield after 15 and 23 days and the larvae final quantity after all tested times (8, 15, and 23 days). After 23 days of larviculture, the yield was highest for 20 larvae mL-1 and the larval number for 45 larvae mL-1. The third experiment also compared two larval densities (100 and 150 larvae... Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.06.03.00-0 bivalve mollusc; Yield; Larviculture; Density. Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/23685 Ocurrence of Vibrio spp., positive coagulase staphylococci and enteric bacteria in oysters (Crassostrea gigas) harvested in the south bay of Santa Catarina island, Brazil Provedor de dados: 116 Autores: Ramos,Roberta Juliano; Pereira,Murilo Anderson; Miotto,Letícia Adélia; Faria,Renata D'Aquino; Silveira Junior,Nelson; Vieira,Cleide Rosana Werneck. The aim of this study was to assess the contamination of oysters (Crassostrea gigas), harvested in six different regions of the South Bay of Santa Catarina Island, with Coliforms at 45 ºC, Escherichia coli, Vibrio spp., positive coagulase staphylococci, and Salmonella sp. over a period of one year. One hundred eighty oyster samples were collected directly from their culture sites and analyzed. Each sample consisted of a pool of 12 oysters. All of the samples analyzed showed absence of Salmonella, 18 (10%) samples showed presence of Escherichia coli, 15 (8.3%) samples were positive for V. alginolyticus, and Vibriocholerae was detected in 4 samples (2.2%). The counts of positive-coagulase staphylococci varied from <10 to 1.9 x 102 CFU.g-1, whereas the... Tipo: Journal article Palavras-chave: Microbiological quality; Bivalve mollusks; Filter-feeders; Escherichia coli; Vibrio parahaemolyticus; Vibrio vulnificus. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000300009 Description de la prodissoconque de Teredo thomsoni Tryon et de Bankia anechoensis Roch Provedor de dados: 177 Autores: Rancurel, Paul. Tipo: Text Palavras-chave: ETUDE COMPARATIVE; ANATOMIE ANIMALE; TARET; CHARNIERE BIVALVE; TEREDO THOMSONI; BANKIA ANECHOENSIS. Ano: 1965 URL: http://www.documentation.ird.fr/hor/fdi:19475 Pectinidés des fonds meubles dans les lagons de Nouvelle-Calédonie et des Chesterfield : 2. Systématique et biogéographie = Pectinidae found on the soft bottom of the New Caledonian and Chesterfield lagoons : 2. Systematic and biogeography Provedor de dados: 177 Autores: Dikstra, H.H.; Richer de Forges, Bertrand; Clavier, Jacques; Lefort, Yves. Tipo: Text Palavras-chave: LAGON; FAUNE MALACOLOGIQUE; FOND MARIN; TAXONOMIE; DESCRIPTION; INVENTAIRE FAUNISTIQUE; REPARTITION GEOGRAPHIQUE; FOND MEUBLE; BIVALVE. Ano: 1990 URL: http://www.documentation.ird.fr/hor/fdi:010025826 Pectinidés des fonds meubles dans les lagons de Nouvelle-Calédonie et des Chesterfield : 1. Présentation et répartition géographique des espèces = Pectinidae found on the soft bottom of the New Caledonian and Chesterfield lagoons : 1. Presentation and geographical distribution of the species Provedor de dados: 177 Autores: Dikstra, H.H.; Richer de Forges, Bertrand; Clavier, Jacques; Lefort, Yves. Tipo: Text Palavras-chave: REPARTITION GEOGRAPHIQUE; FAUNE MALACOLOGIQUE; ESPECE COMMERCIALE; TAXONOMIE; INVENTAIRE FAUNISTIQUE; BIOTOPE; FOND MARIN; FOND MEUBLE; BIVALVE. Ano: 1989 URL: http://www.documentation.ird.fr/hor/fdi:010025825 In situ suspension feeding behaviour of the pearl oyster, Pinctada margaritifera: combined effects of body size and weather-related seston composition Provedor de dados: 5 Autores: Pouvreau, Stephane; Bodoy, Alain; Buestel, Dominique. In situ clearance rate (CR) and biodeposition of the black pearl oyster, Pinctada margaritifera, were followed during several field experiments from 1996 to 1998 in the lagoon of Takapoto. Serial measurements of total particulate matter (TPM, mg l(-1)), particulate inorganic matter (PLM, mg l(-1)), and particulate organic matter (POM, mg l(-1)) were related to meteorological conditions, especially wind speed. As a general case, POM and PLM increased with wind speed. Nevertheless, PLM increased faster than POM so that the organic content (OC, %) of the TPM decreased progressively when wind speed increased. These TPM variations induced direct changes in feeding processes of P. margaritifera. CR (l h(-1)) averaged 22 l h(-1) for a pearl oyster of 1 g dry... Tipo: Text Palavras-chave: Biodeposition; Clearance rate; Seston; Filter feedings behaviour; Tropical lagoon; Tropical bivalve. Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-382.pdf Remodeling of membrane lipids in gills of adult hard clam Mercenaria mercenaria during declining temperature Provedor de dados: 5 Autores: Parent, G; Pernet, Fabrice; Tremblay, R; Sevigny, J; Ouellette, M. In a previous study, a major remodeling of lipids, consistent with the theory of homeoviscous adaptation, was observed in the juvenile hard clam Mercenaria mercenaria during a temperature reduction from 24 to -1 degrees C. In addition, the lipid remodeling varied between genetically distinct lines of hard clams. The present study examined whether adult hard clams originating from different locations and a selectively bred variety show differences related to their genetic characteristics in the remodeling of lipids that normally occur during decreasing temperatures. Wild hard clams from 4 locations in Atlantic Canada and the selectively bred M mercenaria var. notata were held at an aquaculture growout site located at the northern distribution limit of the... Tipo: Text Palavras-chave: Sterol; Phospholipid; Membrane lipid; Intraspecific variation; Food; Fatty acid; Bivalve; Allozymes; Acclimatization. Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4743.pdf Fossil clams from a serpentinite-hosted sedimented vent field near the active smoker complex Rainbow, MAR, 36 degrees 13 ' N: Insight into the biogeography of vent fauna Provedor de dados: 5 Autores: Lartaud, Franck; De Rafelis, Marc; Oliver, Graham; Krylova, Elena; Dyment, Jerome; Ildefonse, Benoit; Thibaud, Remy; Gente, Pascal; Hoise, Eva; Meistertzheim, Anne-leila; Fouquet, Yves; Gaill, Francoise; Le Bris, Nadine. Hydrothermal circulation at ultramafic-hosted sites supports a large variety of high-and low-temperature hydrothermal vents and associated ecosystems. The discovery of abundant fossil vesicomyid and thyasirid shell accumulations at the ridge crest, approximately 2.5 km east of the active Rainbow vent field on the Mid-Atlantic Ridge (MAR, 36 degrees 13'N), increased our knowledge regarding the diversity of vent communities at slow spreading ridges. Bivalve molluscs of the family Vesicomyidae were represented by the genus Phreagena. Here we present the first record of this genus in the Atlantic Ocean. This second vesicomyid species known from the MAR, Phreagena sp., was found to be associated with a Thyasira species that is affiliated with T. southwardae (at... Tipo: Text Palavras-chave: Ultramafic-hosted; Mid-Atlantic Ridge; Bivalve shells; Vesicomyidae; Thyasiridae; Carbon and oxygen isotopes. Ano: 2010 URL: http://archimer.ifremer.fr/doc/00012/12301/9093.pdf Expression of immune-related genes in the oyster Crassostrea gigas during ontogenesis Provedor de dados: 5 Autores: Tirape, Ana; Bacque, Coralie; Brizard, Raphael; Vandenbulcke, Franck; Boulo, Viviane. The work presented here reports the expression of immune-related genes during ontogenesis in the oyster Crassostrea gigar. Expression patterns of 18 selected genes showed that RNAs detected in oocytes and 2-4 cell embryos are of maternal origin and that gene transcription starts early after fertilization. The expression patterns of 4 genes (Cg-timp, Cg-tal, Cg-EcSOD and Drac3) suggested that hemocytes appear in the gastrula-trochophore stages. The localization of Cg-tal expression suggested that hematopoietic cells were derived from vessels and/or artery endothelia cells. Moreover, a bacterial challenge affected the level of expression of genes. Indeed, a change in expression levels was observed for Cg-LBP/BPI, Cg-timp, Drac3 and Cg-MyD88 genes in larval... Tipo: Text Palavras-chave: Rel/NF kappa B pathway; Cell proliferation; Hematopoiesis; Mollusk immunity; Hemocytes; Bivalve mollusk. Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2612.pdf Bivalve hatchery technology: The current situation for the Pacific oyster Crassostrea gigas and the scallop Pecten maximus in France Provedor de dados: 5 Autores: Robert, Rene; Gerard, Andre. The French oyster farming industry relies almost exclusively on juveniles collected from the natural environment; the supply of spat produced by hatcheries is low, about 10% of the industry's requirements. Development through selective breeding of oyster stocks which are better suited for aquaculture purposes, is likely to reverse this tendency since only hatcheries will be able to supply such animals. Scallop farming, which at present is poorly developed in France, relies exclusively on hatchery produced spat. Although hatchery technology is constantly being improved, significant production problems remain which must be solved before hatcheries become a major supplier of juveniles for the industry. This paper describes the present state of hatchery... Tipo: Text Palavras-chave: Pecten maximus; Crassostrea gigas; Hatchery; Molluscan rearing; Pecten maximus; Crassostrea gigas; Ecloserie; Élevage de bivalve. Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/publication-861.pdf Identifying Robust Proxies of Gonad Maturation for the Protandrous Hermaphrodite maxima (Röding, 1798, Bivalvia) from Individual to Population Scale Tridacna Provedor de dados: 5 Autores: Menoud, Mathilde; Van Wynsberge, Simon; Le Moullac, Gilles; Levy, Peva; Andrefouet, Serge; Remoissenet, Georges; Gaertner-mazouni, Nabila. Monitoring gonadmaturation for protandrous and functional hermaphrodite species such as the giant clamTridacna maxima is difficult due to the juxtaposition and relative proportion ofmale and female tissues in the gonad [gonadal sex ratio (GSR)]. Here, the relevance of the widely used gonadosomatic index (GSI) as proxy of giant clam gonad maturation is tested with a large dataset (n = 265). Gonadosomatic index is compared with other indices, namely the proportion of the male part harboring spermatozoids, the proportion of empty oocyte follicles, the mean oocyte diameter, and the oocyte elongation. At gonad scale, high index variability highlighted partial spawning. At individual scale, male and female maturation proxies were contrasted, showing either... Tipo: Text Palavras-chave: Giant clam; Marine bivalve; Reproduction; Spawn; Tatakoto atoll; Histology; Tridacna maxima. Ano: 2016 URL: http://archimer.ifremer.fr/doc/00333/44441/44111.pdf Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPKalpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas Provedor de dados: 5 Autores: Guevelou, Eric; Huvet, Arnaud; Sussarellu, Rossana; Milan, Massimo; Guo, Xinmei; Li, Li; Zhang, Guofan; Quillien, Virgile; Daniel, Jean-yves; Quere, Claudie; Boudry, Pierre; Corporeau, Charlotte. AMP-activated protein kinase α (AMPKα) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated muscles, the amount of full-length AMP-activated protein kinase α (AMPKα) remained unchanged during hypoxia. However, hypoxia induced a rapid and muscle-specific response concerning truncated isoforms of AMPKα. In the smooth muscle, a truncated isoform of AMPKα was increased from 1 to 6 h of hypoxia, and was linked with accumulation of AKT kinase, a key enzyme of the insulin signaling pathway which controls intracellular glucose metabolism. In this muscle, aerobic metabolism... Tipo: Text Palavras-chave: Marine bivalve; Crassostrea gigas; Hypoxia; AMP-activated protein kinase; Alternative splicing; Adductor muscle. Ano: 2013 URL: http://archimer.ifremer.fr/doc/00119/22998/21397.pdf Multi-scale distribution and dynamics of bivalve larvae in a deep atoll lagoon (Ahe, French Polynesia) Provedor de dados: 5 Autores: Thomas, Yoann; Garen, Pierre; Bennett, Auguste; Le Pennec, Marcel; Clavier, Jacques. Bivalve larvae and hydrographic parameters were sampled over a range of spatio-temporal scales in a deep atoll lagoon. Bivalve larvae abundances were very high throughout the year: 18,550 m(-3) in average. Larvae were (i) concentrated at mid-depth with nocturnal ascent and diurnal descent, (ii) heterogeneously dispersed at the lagoon scale, (iii) subject to day-to-day variation in abundance and (iv) transferred between different parts of the lagoon providing evidence of intra-lagoonal connectivity. The primacy of physical factors was seen on large spatial scale with the diluting effect of water renewal and transfers by hydrodynamics. On smaller spatial scale, the primacy of biological processes was recognised, with larval swimming activity leading to dial... Tipo: Text Palavras-chave: Bivalve larvae; Distribution patterns; Vertical migration; Pinctada margaritifera settlement; Deep atoll lagoon; French Polynesia. Ano: 2012 URL: http://archimer.ifremer.fr/doc/00101/21200/20690.pdf Variability in particle retention efficiency by the mussel Mytilus edulis Provedor de dados: 5 Autores: Strohmeier, Tore; Strand, Oivind; Alunno-bruscia, Marianne; Duinker, Arne; Cranford, Peter J. Seasonal variation in blue mussel, Mytilus edulis, retention efficiency (RE) responses to natural seston concentrations were investigated in the Lysefjord, Norway. The smallest particles shown to be effectively retained (100%) were approximately 7 μm and this was observed on only one occasion. The most common response was for RE to progressively increase from small to large particles with maximum RE at 30 to 35 μm (8 of 12 experiments). Temporal changes in the seston size distribution towards a dominance of smaller particles coincided with a decrease in the RE maxima to particles between 7 and 15 μm diameter. The RE of 1 μm and 4 μm diameter particles ranged between 14–64% and 12–86%, respectively, showing that small particles occasionally serve as a... Tipo: Text Palavras-chave: Bivalve feeding physiology; Retention efficiency; Clearance rate; Marine ecophysiology; Seston; Suspension feeding; Mytilus edulis. Ano: 2012 URL: http://archimer.ifremer.fr/doc/00054/16509/14024.pdf Response of Mytilus edulis to enhanced phytoplankton availability by controlled upwelling in an oligotrophic fjord Provedor de dados: 5 Autores: Strohmeier, Tore; Strand, Oivind; Alunno-bruscia, Marianne; Duinker, Arne; Rosland, Rune; Aure, Jan; Erga, Svein R.; Naustvoll, Lars J.; Jansen, Henrice M.; Cranford, Peter J. The controlled upwelling of nutrient-rich deep water in oligotrophic coastal regions has been proposed as a means of increasing phytoplankton and, subsequently, bivalve aquaculture production. This was tested as part of a large-scale upwelling experiment in an oligotrophic environment (Lysefjord, Norway). The mean chlorophyll a concentration in the upwelling area was consistently higher than at the control site (mean ± SD: 3.3 ± 1.9 and 1.5 ± 0.6 mg Chl a m-3, respectively) during the 4 mo of controlled upwelling. After 2 mo with upwelling, the dry flesh weight of 1 yr-old and 2 yr-old mussels was 95% and 24% higher, respectively, than that of the mussels at the control site. The 1 yr-old mussels at the upwelling site achieved up to 2.4-fold higher dry... Tipo: Text Palavras-chave: Bivalve aquaculture; Production carrying capacity; Physiology; Fjord ecosystems; Shell growth; Tissue growth. Ano: 2015 URL: http://archimer.ifremer.fr/doc/00241/35200/33704.pdf Effect of Azadinium spinosum on the feeding behaviour and azaspiracid accumulation of Mytilus edulis Provedor de dados: 5 Autores: Jauffrais, Thierry; Contreras, Andrea; Herrenknecht, Christine; Truquet, Philippe; Sechet, Veronique; Tillmann, Urban; Hess, Philipp. Azadinium spinosum, a small toxic dinoflagellate, was recently isolated and identified as a primary producer of azaspiracid toxins (AZAs). Previous experiments related to AZA accumulation in blue mussels upon direct feeding with A. spinosum revealed increased mussel mortality and had negative effects on the thickness of the digestive gland tubules. Therefore we conducted follow up experiments in order to study effects of A. spinosum on mussel feeding behaviour. Individual assessment of mussel feeding time activity (FTA), clearance rate (CR), filtration rate (TFR), absorption rate (AR), faeces and pseudofaeces production were carried out on mussel fed either toxic (A. spinosum) or non-toxic (Isochrisis aff. galbana (T-Iso)) diets. Furthermore, AZA... Tipo: Text Palavras-chave: Bivalve molluscs; Mussel; Ecophysiology; AZA biotransformation; AZA accumulation; Trophic transfer; Dinoflagellate; Azaspiracid. Ano: 2012 URL: http://archimer.ifremer.fr/doc/00090/20174/17832.pdf A Functional Study of Transforming Growth Factor-Beta from the Gonad of Pacific Oyster Crassostrea gigas Provedor de dados: 5 Autores: Corporeau, Charlotte; Groisillier, Agnes; Jeudy, Alexandra; Barbeyron, Tristan; Fleury, Elodie; Fabioux, Caroline; Czjzek, Mirjam; Huvet, Arnaud. The transforming growth factor (TGF)-beta superfamily is a group of important growth factors involved in multiple processes such as differentiation, cell proliferation, apoptosis and cellular growth. In the Pacific oyster Crassostrea gigas, the oyster gonadal (og) TGF-beta gene was recently characterized through genome-wide expression profiling of oyster lines selected to be resistant or susceptible to summer mortality. Og TGF-beta appeared specifically expressed in the gonad to reach a maximum when gonads are fully mature, which singularly contrasts with the pleiotropic roles commonly ascribed to most TGF-beta family members. The function of og TGF-beta protein in oysters is unknown, and defining its role remains challenging. In this study, we develop a... Tipo: Text Palavras-chave: Transforming growth factor-beta; Bacterial expression; In vivo antibody inhibition; Reproduction; Crassostrea gigas; Marine bivalve. Ano: 2011 URL: http://archimer.ifremer.fr/doc/00042/15371/13438.pdf Effects of extracellular products from the pathogenic Vibrio aestuarianus strain 01/32 on lethality and cellular immune responses of the oyster Crassostrea gigas Provedor de dados: 5 Autores: Labreuche, Yannick; Soudant, Philippe; Goncalves, Madeleine; Lambert, Christophe; Nicolas, Jean-louis. Vibrio aestuarianus strain 01/32 was previously shown to be pathogenic to Crassostrea gigas juveniles. To investigate virulence mechanisms of this pathogen, we studied the toxicity to oysters of its extracellular products (ECPs). ECPs displayed lethality to animals, with a LD50 value of 3.3 mu g/g body weight. To determine the oyster cellular immune responses induced by these ECPs, we further examined in vitro their effects on C. gigas hemocytes, using flow cytometric-based hemocyte assays. Treatment of hemolymph with ECPs caused a significant inhibition of hemocyte phagocytosis and adhesive capabilities. In contrast, the pathway of reactive oxygen species production was enhanced by higher ECP concentrations. Exposure of hemocytes to live bacteria induced... Tipo: Text Palavras-chave: Vibrio aestuarianus; ECPs; Crassostrea gigas; ROS production; Adhesion; Phagocytosis; Flow cytometry; Bivalve immunity. Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1035.pdf Impact of 20 : 4n-6 supplementation on the fatty acid composition and hemocyte parameters of the Pacific oyster Crassostrea gigas Provedor de dados: 5 Autores: Delaporte, Maryse; Soudant, Philippe; Moal, Jeanne; Giudicelli, Emilie; Lambert, Christophe; Seguineau, Catherine; Samain, Jean-francois. Arachidonic acid (20:4n-6, ArA) and its eicosanoid metabolites have been demonstrated to be implicated in immune functions of vertebrates, fish, and insects. Thus, the aim of this study was to assess the impact of ArA supplementation on the FA composition and hemocyte parameters of oysters Crassostrea gigas. Oyster dietary conditioning consisted of direct addition of ArA solutions at a dose of 0, 0.25, or 0.41 mu g ArA per mL of seawater into tanks in the presence or absence of T-Iso algae. Results showed significant incorporation of ArA into gill polar lipids when administered with algae (up to 19.7%) or without algae (up to 12.1%). ArA supplementation led to an increase in hemocyte numbers, phagocytosis, and production of reactive oxygen species by... Tipo: Text Palavras-chave: Hemocyte parameters; Oyster immunity; Arachidonic Acid; Poly unsaturated fatty acids; Nutrition; Bivalve; Crassostrea gigas. Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1876.pdf Appearance and spread of diseases among bivalve molluscs in the northern hemisphere in relation to international trade Provedor de dados: 5 Autores: Renault, Tristan. Bivalve mollusc culture is a well-developed marine aquaculture activity in many countries around the world, notably in the northern hemisphere. During the development of this activity, numerous countries have been confronted with infectious diseases of varying severity and duration. Research has been conducted to determine the aetiology, epidemiology and control measures for these epizootics. Major epizootics in bivalve molluscs have been caused by viruses, bacteria, fungi and protozoan parasites. Moreover, important commercial relations exist in marine mollusc culture between different geographical areas. This must be taken into account in explaining the appearance and the spread of some infectious diseases in several countries around the world. The... Tipo: Text Palavras-chave: Northern hemisphere; International trade; Infectious diseases; Bivalve molluscs; Mollusques bivalves; Maladies infectieuses; Hémisphère Nord; Commerce internatioanal. Ano: 1996 URL: http://archimer.ifremer.fr/doc/1996/publication-2913.pdf Dissolved azaspiracids are absorbed and metabolized by blue mussels (Mytilus edulis) Provedor de dados: 5 Autores: Jauffrais, Thierry; Kilcoyne, Jane; Herrenknecht, Christine; Truquet, Philippe; Sechet, Veronique; Miles, Christopher O.; Hess, Philipp. The relationship between azaspiracid shellfish poisoning and a small dinoflagellate, Azadinium spinosum, has been shown recently. The organism produces AZA1 and -2, while AZA3 and other analogues are metabolic products formed in shellfish. We evaluated whether mussels were capable of accumulating dissolved AZA1 and -2, and compared the toxin profiles of these mussels at 24 h with profiles of those exposed to live or lysed A. spinosum. We also assessed the possibility of preparative production of AZA metabolites by exposing mussels to semi-purified AZA1. We exposed mussels to similar concentration of AZAs: dissolved AZA1+2 (crude extract) at 7.5 and 0.75 µg L-1, dissolved AZA1+2 (7.5 µg L-1) in combination with Isochrysis affinis galbana, and lysed and live... Tipo: Text Palavras-chave: Dissolved marine biotoxins; AZA; Tissue distribution; Bivalve molluscs; LC-MS/MS; Azaspiracid. Ano: 2013 URL: http://archimer.ifremer.fr/doc/00136/24757/22867.pdf Etude histoensymologique de la digestion chez Ruditapes philippinarum Provedor de dados: 5 Autores: Hily, A. Examinations of alimentary tract and digestive tubules after feeding give evidence of continuous digestive process in juveniles of Ruditapes philippinarum . Histoenzymologic study of digestive tract suggests important intracellular enzymatic activities in stomacal epithelium, digestive gland and intestinal cells. Main part of intracellular digestion occurred in digestive cells by lysosyme associated with vacuolar system. Tipo: Text Palavras-chave: Ruditapes philippinarum; Bivalvia; Digestive system; Enzymatic activity; Histology; Digestive glands; Digestion; Enzymes digestives; Digestion; Bivalve. Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1180.pdf Relations entre le contenu lipidique des ovocytes de Pecten maximus (Mollusque, bivalve) et les performances des larves en élevage Provedor de dados: 5 Autores: Le Pennec, Marcel; Gueguen,. The lipid content of spawned oocytes of Pecten maximus was studied by image analysis of semi -thin histological sections and a lipid index was defined. The female gametes were obtained by thermic stimulations of mature adults scallops from different populations after out of season conditioning or sampled in situ during the spawning season. After fertilization, the larval development was followed. The results reveal a large variability in lipid content of normal oocytes. The lipid content can be regarded as homogeneous inside a spawning but the heterogeneity is greater for wild scallops than for conditioned ones. A strong relationship appears between the lipid index and the hatching success, and the number of abnormal larvae after two days rearing. But no... Tipo: Text Palavras-chave: Mollusques; Bivalves; Pecten maximus; Ovocytes; Lipides; Elevages larvaires; Mollusc; Bivalve; Pecten maximus; Oocytes; Lipids; Larval development. Ano: 1990 URL: http://archimer.ifremer.fr/doc/00022/13345/10379.pdf Beta-N-Methylamino-L-Alanine: LC-MS/MS Optimization, Screening of Cyanobacterial Strains and Occurrence in Shellfish from Thau, a French Mediterranean Lagoon Provedor de dados: 5 Autores: Reveillon, Damien; Abadie, Eric; Sechet, Veronique; Brient, Luc; Savar, Veronique; Bardouil, Michele; Hess, Philipp; Amzil, Zouher. beta-N-methylamino-L-alanine (BMAA) is a neurotoxic non-protein amino acid suggested to be involved in neurodegenerative diseases. It was reported to be produced by cyanobacteria, but also found in edible aquatic organisms, thus raising concern of a widespread human exposure. However, the chemical analysis of BMAA and its isomers are controversial, mainly due to the lack of selectivity of the analytical methods. Using factorial design, we have optimized the chromatographic separation of underivatized analogues by a hydrophilic interaction chromatography coupled to tandem mass spectrometry (HILIC-MS/MS) method. A combination of an effective solid phase extraction (SPE) clean-up, appropriate chromatographic resolution and the use of specific mass spectral... Tipo: Text Palavras-chave: Cyanotoxins; BMAA; DAB; AEG; HILIC-MS/MS; Cyanobacteria; Bivalve mollusks; French Mediterranean. Ano: 2014 URL: http://archimer.ifremer.fr/doc/00245/35579/34112.pdf Detection of ostreid herpesvirus 1 DNA by PCR in bivalve molluscs: A critical review Provedor de dados: 5 Autores: Batista, Frederico; Arzul, Isabelle; Pepin, Jean-francois; Ruano, Francisco; Friedman, Carolyn S.; Boudry, Pierre; Renault, Tristan. Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1... Tipo: Text Palavras-chave: Oyster; Bivalve molluscs; PCR; Detection; OsHV 1; Herpesvirus. Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2322.pdf Combination of a pesticide exposure and a bacterial challenge: In vivo effects on immune response of Pacific oyster, Crassostrea gigas (Thunberg) Provedor de dados: 5 Autores: Gagnaire, Beatrice; Gay, Melanie; Huvet, Arnaud; Daniel, Jean-yves; Saulnier, Denis; Renault, Tristan. To assess the impact of pollution induced by pesticides on Pacific oyster, Crassostrea gigas, health in France, in vivo effects of combined pesticide exposure and bacterial challenge on cell activities and gene expression in hemocytes were tested using flow cytometry and real-time PCR. As a first step, an in vivo model of experimental contamination was developed. Pacific oysters were exposed to a mixture of eight pesticides (atrazine, glyphosate, alachlor, metolachlor, fosetyl-alumimium, terbuthylazine, diuron and carbaryl) at environmentally relevant concentrations over a 7-day period. Hemocyte parameters (cell mortality, enzyme activities and phagocytosis) were monitored using flow cytometry and gene expression was evaluated by real-time PCR (RT-PCR).... Tipo: Text Palavras-chave: Gene expression; Flow cytometry; Vibrio splendidus; Pathogenesis; Phagocytosis; Hemocyte; Pesticides; Bivalve immunity; Crassostrea gigas; Pacific oyster. Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3022.pdf In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas Provedor de dados: 5 Autores: Huvet, Arnaud; Fleury, Elodie; Corporeau, Charlotte; Quillien, Virgile; Daniel, Jean-yves; Riviere, Guillaume; Boudry, Pierre; Fabioux, Caroline. We investigated the role of oyster gonadal TGF beta (og-TGF beta) in the reproduction of Crassostrea gigas, using an in vivo RNA interference approach. We designed double-stranded RNA targeting og-TGF beta, which is specifically expressed in the somatic cells surrounding germ cells in the gonad of both male and female oysters. In vivo injection of this og-TGF beta dsRNA into the gonad led to knock-down phenotypes for both sexes, with significant reduction (77.52% relative to controls) of the gonad area, lowered reproductive effort and germ cell under-proliferation. Interestingly, half of the injected females halted their vitellogenesis, since we were only able to observe pre-vitellogenic oocytes. In addition, apoptotic germ cells and haemocytes infiltrated... Tipo: Text Palavras-chave: Marine bivalve; Crassostrea gigas; Reproduction; RNA interference; Transforming growth factor-beta. Ano: 2012 URL: http://archimer.ifremer.fr/doc/00087/19794/17486.pdf Influence du mode de stockage sur la toxicité d'une eau et d'un sédiment à l'aide du bio-essai embryon de bivalve - Etude préliminaire Provedor de dados: 5 Autores: Damee, Nicolas. La conservation des échantillons a souvent constitué un problème tant pour les chimistes que pour les biologistes. Si les chimistes semblent satisfaits de leurs techniques de conservation, les biologistes quant à eux sont indécis du fait que l'action toxique se modifie au cours du temps et selon la technique employée (réfrigération, congélation, lyophilisation, conservation en azote liquide à -196°C). Le présent rapport constitue une étude préliminaire qui, si elle est poursuivie, pourrait permettre de déterminer le mode de conservation le plus approprié pour chaque type d'échantillons. Tipo: Text Palavras-chave: Conservation; Bio-essai; Pourcentage net d'anomalies (PNA); Sédiment; Bivalve. Ano: 1993 URL: http://archimer.ifremer.fr/doc/00037/14828/12144.pdf Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas Provedor de dados: 5 Autores: Guevelou, Eric; Huvet, Arnaud; Galindo-sanchez, Clara E.; Milan, Massimo; Quillien, Virgile; Daniel, Jean-yves; Quere, Claudie; Boudry, Pierre; Corporeau, Charlotte. The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previously characterized; we identified AMPK beta, AMPK gamma, and mRNAs of putative AMPK-related targets following bioinformatics mining on existing genomic resources. We analyzed the mRNA expression of the AMPK alpha, beta, and gamma subunits in the gonads of male and female oysters through a reproductive cycle, and we quantified the mRNA expression of genes belonging to fatty acid and glucose metabolism. AMPK alpha mRNA levels were more abundant in males at the... Tipo: Text Palavras-chave: AMP-activated protein kinase; Crassostrea gigas; Gametogenesis; Marine bivalve; Reproduction. Ano: 2013 URL: http://archimer.ifremer.fr/doc/00168/27911/26470.pdf Temperature and seston quantity and quality effects on field reproduction of farmed oysters, Crassostrea gigas, in Bourgneuf Bay, France Provedor de dados: 5 Autores: Dutertre, Mickael; Beninger, Peter G.; Barille, Laurent; Papin, Mathias; Rosa, Philippe; Barille, Anne-laure; Haure, Joel. The proliferation of the voluntarily-introduced cupped oyster, Crassostrea gigas, has attained the proportions of species invasion in many intertidal habitats in Europe, presumably resulting from successful reproduction of farmed individuals. It is thus imperative to better understand the reproductive characteristics of farmed oysters, since they are directly under human control. We quantified the dry tissue mass (DTM), gametosomatic index (GSI), and reproductive cycle of farmed oysters at two sites in Bourgneuf Bay, France, in relation to environmental parameters using continuously-recording probes in 2005 and 2006. The GSI was developed for this study, based on the actual area occupied by gametes, rather than the area of the gonad previously used for... Tipo: Text Palavras-chave: Atlantic Ocean; Crassostrea gigas; Oyster farming; Bivalve; Gametosomatic index; Biological invasion; Atresia; Oocytes; Turbidity; Temperature; Reproduction. Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6871.pdf Feeding rate of the oyster Crassostrea gigas in a natural planktonic community of the Mediterranean Thau Lagoon Provedor de dados: 5 Autores: Dupuy, Christine; Vaquer, André; Lam Hoai, Thong; Rougier, Claude; Mazouni, Nabila; Lautier, Jacques; Collos, Yves; Le Gall, Solange. The Mediterranean Thau Lagoon is an important oyster farming area in Europe. Oyster growth rates are among the highest in France, although chlorophyll a concentration is low. Previous studies have demonstrated that picophytoplankton, nano-microphytoplankton, dinoflagellates and loricate ciliates such as tintinnids are abundant. However, heterotrophic flagellates and aloricate ciliates have not been investigated. The aim of this study was to assess picophytoplankton, protist and zooplankton abundances in the Lagoon and to investigate the particular structure of the microbial food web, which may explain such paradoxical oyster growth. In oligotrophic waters in the Thau Lagoon, the picoeukaryote Ostreococcus tauri is the dominant autotrophic picoplankter,... Tipo: Text Palavras-chave: Trophic link; Picophytoplankton; Heterotrophic protist; Microbial food web; Thau Lagoon; Food source; Oyster; Bivalve. Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-812.pdf Impact of the oyster Crassostrea gigas on a microbial community in Atlantic coastal ponds near La Rochelle Provedor de dados: 5 Autores: Dupuy, Christine; Pastoureaud, Annie; Ryckaert, Mireille; Sauriau, Pierre-guy; Montanie, Hélène. To assess the in situ impact of oysters Crassostrea gigas on planktonic protist and bacteria communities and the potential contribution of protozoa to their food resource intake, the abundance and the diversity of protists and bacteria were followed in 2 Atlantic coastal ponds, with and without oysters. The protist biomass in such ponds was high, with a maximum in spring of 982 mug C l(-1) and a minimum in winter of 179 pg C l(-1). Whatever the season, the presence of oysters (20 m(-2) corresponding to an average of 23 mg dry weight m(-2)) induced a significant decrease in >5 pm protist abundance. On the contrary, planktonic organisms <5 pm, such as Chlorophyta flagellates and bacteria, developed similarly in both ponds. It can be assumed that such... Tipo: Text Palavras-chave: Trophic link; Bacteria; Protists; Microbial food web; Coastal pond; Food source; Oysters; Bivalve. Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-822.pdf Physiological responses of Manila clams Venerupis (=Ruditapes) philippinarum with varying parasite Perkinsus olseni burden to toxic algal Alexandrium ostenfeldii exposure Provedor de dados: 5 Autores: Lassudrie, Malwenn; Soudant, Philippe; Richard, Gaelle; Henry, N.; Medhioub, Walid; Da Silva, P.m.; Donval, Anne; Bunel, M.; Le Goïc, Nelly; Lambert, Christophe; De Montaudoin, X.; Fabioux, Caroline; Hegaret, Helene. Manila clam stock from Arcachon Bay, France, is declining, as is commercial harvest. To understand the role of environmental biotic interactions in this decrease, effects of a toxic dinoflagellate, Alexandrium ostenfeldii, which blooms regularly in Arcachon bay, and the interaction with perkinsosis on clam physiology were investigated. Manila clams from Arcachon Bay, with variable natural levels of perkinsosis, were exposed for seven days to a mix of the nutritious microalga T-Iso and the toxic dinoflagellate A. ostenfeldii, a producer of spirolides, followed by seven days of depuration fed only T-Iso. Following sacrifice and quantification of protozoan parasite Perkinsus olseni burden, clams were divided into two groups according to intensity of the... Tipo: Text Palavras-chave: Bivalve; Harmful algal bloom; Oxidative stress; Venerupis philippinarum; Alexandrium ostenfeldii; Perkinsus olseni. Ano: 2014 URL: http://archimer.ifremer.fr/doc/00189/30022/28507.pdf Polymorphisme enzymatique de trois populations de palourdes (Ruditapes decussatus L.) de Bretagne Provedor de dados: 5 Autores: Moraga, D; Lucas, A. The three populations studied were situated on the west coast of Brittany: to the North (Diben), to the West (Tinduff) to the South (Ile Tudy). 25 to 57 analysis were carried out on each of the 19 enzymatic systems where 27 loci had been recognized. Six of which were not interpreted, 10 were monomorphic, 11 polymorphic. Among these latter, the analysis of 4 of the loci were not used as the determination was too confused. Therefore, the comparison of the 3 populations was carried out on 7 polymorphic loci (EST3, HK2, LAP2, MDH1, MDH2, GPI, PGM2). Only a rare allele showed one qualitative difference; this coupled with slight quantitative differences in the distribution of the alleles did not permit to distinguish the three various populations. The genetic... Tipo: Text Palavras-chave: ANE; France; Brittany Ruditapes decussatus Bivalvia Genetics Enzymes Biopolymorphism Bivalve Mollusque Heterozygotie Polymorphisme enzymatique. Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1183.pdf Disruption of amylase genes by RNA interference affects reproduction in the Pacific oyster Crassostrea gigas Provedor de dados: 5 Autores: Huvet, Arnaud; Beguel, Jean-philippe; Cavaleiro, Nathalia; Thomas, Yoann; Quillien, Virgile; Boudry, Pierre; Alunno-bruscia, Marianne; Fabioux, Caroline. Feeding strategies and digestive capacities can have important implications for variation in energetic pathways associated with ecological and economically important traits, such as growth or reproduction in bivalve species. Here, we investigated the role of amylase in the digestive processes of Crassostrea gigas, using in vivo RNA interference. This approach also allowed us to investigate the relationship between energy intake by feeding and gametogenesis in oysters. Double-stranded (ds)RNA designed to target the two α-amylase genes A and B was injected in vivo into the visceral mass of oysters at two doses. These treatments caused significant reductions in mean mRNA levels of the amylase genes: −50.7% and −59% mRNA A, and −71.9% and −70.6% mRNA B in 15... Tipo: Text Palavras-chave: Alpha-Amylase; DsRNA; Gametogenesis; Marine bivalve; Dynamic energy budget. Ano: 2015 URL: http://archimer.ifremer.fr/doc/00271/38239/36422.pdf Polyploidy in the Manila clam Ruditapes philippinarum - 1 - Chemical induction and larval performances of triploids Provedor de dados: 5 Autores: Dufy, Christian; Diter, Alain. An effective treatment using catochalasin B (1 mg CB/ml dimethylsulfoxyde in 1 litre of sea water) was developed to induce triploidy in the Manila clam Ruditapes philippinurum (Adams and Reeve). The percentage of induced triploidy was assessed by karyological examination of embryos and spat. The treatment was found to be optimal between 20 to 35 minutes post-insemination exposure to CB, at 25'C, and produced 75.8 ± 5.7% triploid embryos on the average over four experiments. Growth and survival of larvae to metamorphosis were recorded for two different rearings each split into two replicates. Both larval rearings showed similar survival up to settlement: 19.3 and 27.0%, on average over two replicates, in the treated batches relative to the controls. Mean... Tipo: Text Palavras-chave: Larval survival; Larval growth; Bivalves; Cytochalasin B; Triploidy; Sruvie larvaire; Croissance larvaire; Bivalve; Cytochalasine B; Triploïdie. Ano: 1990 URL: http://archimer.ifremer.fr/doc/1990/publication-2752.pdf French scallops: A new host for ostreid herpesvirus-1 Provedor de dados: 5 Autores: Arzul, Isabelle; Nicolas, Jean-louis; Davison, Andrew J.; Renault, Tristan. Sporadic high mortalities were reported among larval French scallops (Pecten maximus). Electron microscopy of moribund larvae revealed particles with the characteristics of a herpesvirus in association with cellular lesions. PCR and DNA sequencing showed that the virus is a variant of ostreid herpesvirus-1 that has already been described in clams and oysters. This is the first description of a herpesvirus infection of a scallop species. The virus was transmitted successfully from an extract of infected scallop larvae to uninfected scallop or oyster (Crassostrea, gigas) larvae, demonstrating that it is able to infect both species. Detection of viral DNA in asymptomatic adult scallops by in situ hybridisation indicates that the herpesvirus may have been... Tipo: Text Palavras-chave: Bivalve; Host range; Transmission; Polymorphism; OsHV 1; Pecten maximus; Scallop; Herpesvirus. Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-739.pdf Genetic structure at different spatial scales in the pearl oyster (Pinctada margaritifera cumingii) in French Polynesian lagoons: beware of sampling strategy and genetic patchiness Provedor de dados: 5 Autores: Arnaud, Sophie; Vonau, Vincent; Rouxel, Catherine; Bonhomme, Francois; Prou, Jean; Goyard, Emmanuel; Boudry, Pierre. In order to study further the genetic structure of the pearl oyster Pinctada margaritifera in French Polynesia with a special consideration for the sampling scale, we analyzed or re-analyzed sets of data based on nuclear DNA markers obtained at different spatial scales. At a large scale (several 1,000 km), the remote Marquesas Islands were confirmed to be significantly differentiated from Tuamotu-Gambier and Society archipelagos, with a marked difference however for the two main islands that are different from each other. At a medium scale (several 10 to several 100 km), overall homogeneity was observed within and between these two archipelagos, with some exceptions. This could be attributed both to large-scale larval dispersal and to human-driven spat... Tipo: Text Palavras-chave: Population genetics; Polynesia; Bivalve; Pearl oyster; Benthic organisms; Sampling strategy; Genetic patchiness. Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4960.pdf Effect of a mono-specific algal diet on immune functions in two bivalve species - Crassostrea gigas and Ruditapes philippinarum Provedor de dados: 5 Autores: Delaporte, Maryse; Soudant, Philippe; Moal, Jeanne; Lambert, Christophe; Quere, Claudie; Miner, Philippe; Choquet, Gwenaelle; Paillard, Christine; Samain, Jean-francois. The impact of diets upon the fatty acid composition of haemocyte polar lipids and consequently upon immune parameters has been tested in the oyster Crassostrea gigas and the clam Ruditapes philippinarum. Oysters and clams were fed each of three cultured algae: Chaetoceros calcitrans, which is rich in 20:5(n-3) and 20:4(n-6) and poor in 22:6(n-3) fatty acids; T-Iso (Isochrysis sp.), which is rich in 22:6(n-3) and deficient in 20:5(n-3) and 20:4(n-6); and Tetraselmis suecica, which is deficient in 22:6(n-3) and contains only small amounts of 20:5(n-3) and 20:4(n-6). Fatty acid composition of haemocyte polar lipids was greatly affected by the diet. Oysters and clams fed C. calcitrans maintained a higher proportion of 20:5(n-3) and 20:4(n-6) in their haemocyte... Tipo: Text Palavras-chave: Ruditapes philippinarum; Crassostrea gigas; Polyunsaturated fatty acids; Phagocytosis; Oxidative activity; Haemocyte membrane; Nutrition; Immunology; Bivalve; Immunologie; Bivalve; Ruditapes philippinarum; Crassostrea gigas; Acides gras poly insatures; Phagocytose; Flambee respiratoire; Membrane; Hemocyte; Nutrition. Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-354.pdf Le stock naturel de nacre PINCTADA MARGARITIFERA L. dans l'atoll de Scilly (Archipel de la Société - Polynésie Française) Provedor de dados: 177 Autores: Intès, André; Laboute, Pierre; Coeroli, M. Tipo: Text Palavras-chave: ATOLL; PERLICULTURE; NACRE; MILIEU NATUREL; ESTIMATION DE STOCK; ECHANTILLONNAGE; BIOTOPE; BIOMASSE; DYNAMIQUE DE POPULATION; DISTRIBUTION SPATIALE; MOLLUSQUE BIVALVE; DISTRIBUTION TAILLE. Ano: 1986 URL: http://www.documentation.ird.fr/hor/fdi:23130 Contribution à l'étude de virus de mollusques marins apparentés aux Iridoviridae et aux Herpesviridae Provedor de dados: 5 Autores: Le Deuff, Rose-marie. Among the marine molluscs, Pacific oyster, Crassostrea gigas, is actually the most reared species in the world. Noticeably in France, where it was imported between 1960 and 1970, and replaced Portuguese oyster, Crassostrea angulata. Intensification of breeding and the associated introductions of new species may be hazardous. Indeed, they might be responsible for the appearance and the dissemination of new diseases in bivalve molluscs. In addition to these observations, the quasi monobreeding of C. gigas in France enhances the necessity to develop research concerning the diagnosis of pathologies affecting this species, namely the diagnosis of viral diseases. Several families of viruses have been described within marine bivalves molluscs. The purpose of this... Tipo: Text Palavras-chave: Mollusques bivalves; Crassostrea gigas; Huître japonaise; Pathologie; Iridovirus; Herpèsvirus; Diagnostic; Bivalve molluscs; Crassostrea gigas; Pacific oyster; Pathology; Iridovirus; Herpesvirus; Diagnosis. Ano: 1995 URL: http://archimer.ifremer.fr/doc/00107/21792/19367.pdf Expression, tissue localization and synergy of antimicrobial peptides and proteins in the immune response of the oyster Crassostrea gigas Provedor de dados: 5 Autores: Schmitt, Paulina; De Lorgeril, Julien; Gueguen, Yannick; Destoumieux-garzon, Delphine; Bachere, Evelyne. Diverse families of antimicrobial peptides and proteins have been described in oysters. We investigated here how antimicrobials are involved in the immune response against a pathogenic strain of Vibrio splendidus. Oyster antimicrobials were shown to display a wide variety of expression profiles in hemocyte populations and tissues. Oyster defensins are constitutively expressed in specific tissues such as mantle (Cg-Defm) or hemocytes (Cg-Defhs), while Cg-BPI is inducible and Cg-Prp appears down-regulated in hemocytes upon infection. The migratory behavior of hemocytes that express the different antimicrobials was found to be involved in the oyster response to a pathogenic Vibrio infection. Indeed, it contributes to colocalize several antimicrobials that... Tipo: Text Palavras-chave: Pacific oyster; Bivalve mollusk; Innate immunity; Bactericidal/permeability-increasing protein; Proline-rich peptide; Defensin; Vibrio. Ano: 2012 URL: http://archimer.ifremer.fr/doc/00093/20382/18267.pdf Azaspiracid accumulation, detoxification and biotransformation in blue mussels (Mytilus edulis) experimentally fed Azadinium spinosum Provedor de dados: 5 Autores: Jauffrais, Thierry; Marcaillou-le Baut, Claire; Herrenknecht, Christine; Truquet, Philippe; Sechet, Veronique; Nicolau, Elodie; Tillmann, Urban; Hess, Philipp. Azadinium spinosum (Elbrächter and Tillmann), a small marine dinoflagellate, has been recently described as a de novo producer of azaspiracid-1 and -2 (AZA1 and -2) diarrhoeic toxins. A culture of A. spinosum was established in our laboratory and optimised for pilot-scale production of this organism, to evaluate and understand AZA1 and -2 accumulation and biotransformation in blue mussels (Mytilus edulis) fed with A. spinosum. Adult mussels were continuously exposed to A. spinosum over 1 week in 160 L cylindrical conical tanks. Three different diets were tested for contamination: 5000, 10 000 cells mL−1 of A. spinosum and a mixture of 5000 cells mL−1 of A. spinosum with 5000 cells mL−1 of Isochrysis aff. galbana (T-Iso, CCAP 927/14). During the subsequent... Tipo: Text Palavras-chave: Azaspiracid; Azadinium spinosum; Marine biotoxins; AZA; Tissue distribution; Histology; Bivalve molluscs; Liquid chromatography coupled to tandem mass spectrometry. Ano: 2012 URL: http://archimer.ifremer.fr/doc/00088/19877/17566.pdf Morphological Trends of Four Manila Clam Populations (Venerupis philippinarum) on the French Atlantic Coast: Identified Spatial Patterns and Their Relationship to Environmental Variability Provedor de dados: 5 Autores: Caill-milly, Nathalie; Bru, Noelle; Barranger, Melanie; Gallon, Laurent; D'Amico, Franck. For species' dynamics highly influenced by environmental conditions, sustainable management for conservation and/or exploitation purposes requires the ability to segregate natural processes from anthropogenic impacts. In this context, identification of phenotypic variations in morphological patterns, and clarification of the mechanisms involved, constitute useful tools to identify adequate management measures from Europe-wide to local conditions. Although such approaches are already used effectively in the management of fish stocks, they are more recent for bivalve stocks, which are also good candidates for such management tools. The current study examines morphological patterns in the Manila clam (Venerupis philippinarum) through morphometric studies of 4... Tipo: Text Palavras-chave: Shell shape; Linear morphometric methods; Contour detection; Environmental effect; Linear multilevel model; Bivalve; Atlantic Ocean; Venerupis philippinarum. Ano: 2014 URL: http://archimer.ifremer.fr/doc/00225/33605/32004.pdf Culturally significant fisheries: keystones for management of freshwater social-ecological systems Provedor de dados: 7 Autores: Noble, Mae; Fenner School of Environment and Society, The Australian National University; [email protected]; Duncan, Phil; Gamilaroi Traditional Owner, NSW Aboriginal Land Council; [email protected]; Perry, Darren; Murray Lower Darling Rivers Indigenous Nations; [email protected]; Prosper, Kerry; Paq'tnekek Mi'kmaq First Nations; [email protected]; Rose, Denis; Gunditj Mirring Traditional Owners Aboriginal Corporation; [email protected]; Schnierer, Stephan; School of Environment, Science and Engineering, Southern Cross University; [email protected]; Tipa, Gail; Tipa and Associates Ltd.; [email protected]; Williams, Erica; National Institute of Water and Atmospheric Research; [email protected]; Woods, Rene; National Cultural Flows Program; Murray Lower Darling River Indigenous Nations; [email protected]; Pittock, Jamie; Fenner School of Environment and Society, The Australian National University; [email protected]. Indigenous peoples of North America, Australia, and New Zealand have a long tradition of harvesting freshwater animals. Over generations of reliance and subsistence harvesting, Indigenous peoples have acquired a profound understanding of these freshwater animals and ecosystems that have become embedded within their cultural identity. We have identified trans-Pacific parallels in the cultural significance of several freshwater animal groups, such as eels, other finfish, bivalves, and crayfish, to Indigenous peoples and their understanding and respect for the freshwater ecosystems on which their community survival depends. In recognizing such cultural connections, we found that non-Indigenous peoples can appreciate the deep significance of freshwater animals... Tipo: Peer-Reviewed Synthesis Palavras-chave: Adaptive freshwater management; Aquatic resources; Bivalve; Comanagement; Crayfish; Cultural keystone species; Eel; Indigenous ecological knowledge; Indigenous water rights; Lamprey; Salmon; Social-ecological resilience. Ano: 2016 Аллометрия раковины двустворчатых моллюсков Provedor de dados: 13 Autores: Заика, В. Е. Для представителей девяти родов двустворчатых моллюсков (морских и пресноводных) приведены значения показателя степени в аллометрических уравнениях, связывающих массу раковины с общей сырой массой животного. Все исследованные случаи соответствуют положительной либо слабо отрицательной аллометрии, граничащей с изометрией. Приведенные данные расширяют фактическую основу, на которой базируется известное заключение о положительной аллометрии скелетных структур (как внутренних, так и наружных). В связи с этим, более общее значение получает и концепция автора о том, что положительная аллометрия скелета (и других опорных структур) является основной причиной отрицательной метаболической аллометрии (энергетического обмена). Tipo: Journal Contribution Palavras-chave: Двустворчатые моллюски; Раковина; Аллометрия; Bivalve mollusks; Shell; Allometry Allometry Shells Allometry http://aims.fao.org/aos/agrovoc/c_24962. Ano: 2004 URL: http://repository.ibss.org.ua/dspace/handle/99011/67 High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas Provedor de dados: 5 Autores: Rosa, Rafael D.; Alonso, Pascal; Santini, Adrien; Vergnes, Agnes; Bachere, Evelyne. We report here the first evidence in an invertebrate, the oyster Crassostrea gigas, of a phenomenon of Presence-Absence Variation (PAV) affecting immune-related genes. We previously evidenced an extraordinary interindividual variability in the basal mRNA abundances of oyster immune genes including those coding for a family of antimicrobial peptides, the big defensins (Cg-BigDef). Cg-BigDef is a diverse family composed of three members: Cg-BigDef1 to -3. Here, we show that besides a high polymorphism in Cg-BigDef mRNA expression, not all individual oysters express simultaneously the three Cg-BigDefs. Moreover, in numerous individuals, no expression of Cg-BigDefs could be detected. Further investigation at the genomic level revealed that in individuals in... Tipo: Text Palavras-chave: Mollusk bivalve; Antimicrobial peptide; Polymorphism; Gene copy number variation; High-throughput microfluidic RT-qPCR; Vibrio infection. Ano: 2015 URL: http://archimer.ifremer.fr/doc/00239/35009/33545.pdf An ecosystem-based approach and management framework for the integrated evaluation of bivalve aquaculture impacts Provedor de dados: 5 Autores: Cranford, Peter J.; Kamermans, Pauline; Krause, Gesche; Mazurie, Joseph; Buck, Bela H.; Dolmer, Per; Fraser, David; Van Nieuwenhove, Kris; O'Beirn, Francis X.; Sanchez-mata, Adoracion; Thorarinsdottir, Gudrun G.; Strand, Oivind. An ecosystem-based approach to bivalve aquaculture management is a strategy for the integration of aquaculture within the wider ecosystem, including human aspects, in such a way that it promotes sustainable development, equity, and resilience of ecosystems. Given the linkage between social and ecological systems, marine regulators require an ecosystem-based decision framework that structures and integrates the relationships between these systems and facilitates communication of aquaculture-environment interactions and policy-related developments and decisions. The Drivers-Pressures-State Change-Impact-Response (DPSIR) management framework incorporates the connectivity between human and ecological issues and would permit available performance indicators to... Tipo: Text Palavras-chave: Bivalve aquaculture management; Ecosystem-based approach; DPSIR framework; Indicators; Thresholds; Benthic effects; Pelagic effects; Social-ecological systems. Ano: 2012 URL: http://archimer.ifremer.fr/doc/00099/20997/18624.pdf A hemocyte gene expression signature correlated with predictive capacity of oysters to survive Vibrio infections Provedor de dados: 5 Autores: Rosa, Rafael Diego; De Lorgeril, Julien; Tailliez, Patrick; Bruno, Roman; Piquemal, David; Bachere, Evelyne. Background: The complex balance between environmental and host factors is an important determinant of susceptibility to infection. Disturbances of this equilibrium may result in multifactorial diseases as illustrated by the summer mortality syndrome, a worldwide and complex phenomenon that affects the oysters, Crassostrea gigas. The summer mortality syndrome reveals a physiological intolerance making this oyster species susceptible to diseases. Exploration of genetic basis governing the oyster resistance or susceptibility to infections is thus a major goal for understanding field mortality events. In this context, we used high-throughput genomic approaches to identify genetic traits that may characterize inherent survival capacities in C. gigas. Results:... Tipo: Text Palavras-chave: Marine invertebrate; Mollusk bivalve; Mass mortality; Transcriptome-wide analysis; Digital gene expression; Microfluidic qPCR; Survival signature; Polymorphism; Gene copy number; Survival predictive biomarkers. Ano: 2012 URL: http://archimer.ifremer.fr/doc/00095/20624/18262.pdf Les maladies des mollusques bivalves: risques et prévention Provedor de dados: 5 Autores: Grizel, Henri. La conchyliculture basée sur les activités de pêche et d'élevage dans le milieu naturel s'est considérablement développée au cours de ces dernières années, notamment grâce à la production de naissain dans les écloseries et les nurseries, L'accroissement des demandes des échanges de produits, facilité par les moyens modernes de transport, a provoqué une augmentation des risques de propagation de maladies. En s'appuyant sur des exemples, une analyse est faite sur les risques encourus liés aux pratiques conchylicoles et à l'état actuel des connaissances scientifiques. Des recommandations sont formulées sur la prévention, sur le besoin de réglementations et sur les difficultés rencontrées dans ce domaine, Enfin, quelques suggestions sont proposées pour mieux... Tipo: Text Palavras-chave: Analyse des risques; Commerce international; Conchyliculture; Maladies; Mollusques bivalves; Santé animale.; Animal health; Bivalve molluscs; Diseases; International trade; Risk analysis; Shellfish farming. Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/publication-6089.PDF Contribution à l'étude du système neuroendocrine de la moule Mytilus edulis (Mollusca: Bivalvia) Provedor de dados: 5 Autores: Kellner-cousin, Kristell. Dans le cadre de ce travail, l'étude du système neuroendocrine de la moule Mytilus edulis a été envisagée sous plusieurs aspects qui définissent les quatre grandes parties de la thèse. Dans une première partie est décrit le travail de préparation et de caractérisation d'anticorps monoclonaux (AcMc) spécifiques de ganglions cérébroïdes (GC) de Mytilus edulis. Le développement de techniques de préparation d'AcMc dirigés contre des antigènes non purifiés a en effet permis de produire un ensemble de réactifs de type AcMc dirigés contre différents antigènes issus d'un broyat de GC de moule. La spécificité de ces AcMc a été étudiée en microscopie optique puis électronique selon des techniques immunocytochimiques (immunopéroxydase indirecte et immunogold). La... Tipo: Text Palavras-chave: Mollusque; Bivalve; Mytilus edulis; Cellules neurosécrétrices; Système nerveux; Système immunitaire; Controle de la croissance; Insuline; Anticorps monoclonaux; Immunocytocbimie; ELISA; PCR; Banque d'ADNc. Ano: 1993 URL: http://archimer.ifremer.fr/doc/00109/22019/19642.pdf Menai Governance Baseline Provedor de dados: 20 Autores: Torell, Elin; Mmochi, Aviti; Palmigiano, Karen. Menai Bay Conservation Area (MBCA) is situated in the southwest of Unguja, the main island of the Zanzibar Archipelago, and covers an area of 467 km² including 6 islets, with a seaward boundary close to 61 km offshore. It is the largest marine conservation area in Zanzibar, managed locally by the community and government officials with technical assistance by the World Wild Fund (WWF). The area is extensively covered with coral reefs, sea grass beds, and mangrove forest. The average water depth is 10 meters at high tide. The area had remained relatively undisturbed until the mid-1990s, when uncontrolled fishing pressures combined with destructive fishing techniques became a serious environmental concern Tipo: Report Palavras-chave: Coastal area conservation; Turtle conservation; Bivalve farming Baseline studies Fishery management Fishery management http://aims.fao.org/aos/agrovoc/c_24026. Ano: 2006 URL: http://hdl.handle.net/1834/1326 Etude des interactions hôte/virus chez l’huître creuse Crassostrea gigas et son virus Ostreid herpesvirus 1 Provedor de dados: 5 Autores: Segarra, Amelie. Ostreid herpesvirus type 1 (OsHV-1) can be considered one of the major infectious agents in Pacific oysters, Crassostrea gigas, in France and all around the world. Differences in terms of mortality among infected animals were reported suggesting a genetic basis of the susceptibility to the OsHV-1 infection. In this context, the main objective of this thesis was to understand the interactions between Pacific oysters and OsHV-1, in particular, the molecular basis of the viral cycle. Results showed that the virus is able to replicate in the host regardless of its stage of development or its susceptibility. However, multiplication kinetics is faster in the most susceptible individuals compared to less susceptible ones. After an active replication phase, it... Tipo: Text Palavras-chave: Bivalves; Huître creuse; Crassostrea gigas; Mortalités; OsHV-1; Gènes viruax; Interaction hôte-virus; Transcriptome; Sensibilité; PCR en temps réel; Bivalve; Pacific oyster; Crassostrea ggias; Mortality; OsHV-1; Viral gene; Interaction host-virus; Transcriptome; Susceptibility; Real-time PCR. Ano: 2014 URL: http://archimer.ifremer.fr/doc/00252/36297/34826.pdf