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Sunlight energy Energy Flow and Matter Cycling (Carbon) ECOSYSTEM Photosynthesis in chloroplasts CO2 Glucose + + H2O O2 Cellular respiration in mitochondria ATP (for cellular work) Heat energy Cellular Respiration • The process by which an organism can extract energy from organic molecules (ex: glucose) and use that energy to make ATP Cellular Respiration has 3 parts • Glycolysis • Krebs cycle • Oxidative phosphorylation (AKA the electric transport chain) Quick overview of the Cell More detail of a plant cell Structure of the Mitochondria Glycolysis • • • • Occurs in the cytoplasm of the cell ATP is required to start the process Starts with glucose (6 carbons) produces 2 pyruvate, AKA pyruvic acid(3 carbons each) • What do you gain (energy) at the end? – ATP (2) – NADH • This is an electron carrier that will be used later Fig. 6-7a Glucose 2 ADP 2 NAD+ + 2 P 2 NADH 2 ATP + 2 H+ 2 Pyruvate Fig. 6-7c ENERGY INVESTMENT PHASE Glucose ATP Steps 1 – 3 A fuel molecule is energized, using ATP. Step 1 ADP P Glucose-6-phosphate P Fructose-6-phosphate P Fructose-1,6-bisphosphate 2 ATP 3 ADP P Step 4 A six-carbon intermediate splits Into two three-carbon intermediates. 4 P Step 5 A redox reaction generates NADH. Glyceraldehyde-3-phosphate (G3P) P NAD+ NAD+ 5 P NADH 5 NADH + H+ ENERGY PAYOFF PHASE P + H+ P P ADP P P 1,3-Bisphosphoglycerate ADP 6 6 ATP ATP P P 3-Phosphoglycerate 7 Steps 6 – 9 ATP and pyruvate are produced. 7 P P 2-Phosphoglycerate 8 H2 O P P ADP Phosphoenolpyruvate (PEP) ADP 9 ATP 8 H2 O 9 ATP Pyruvate Modeling Glycolysis • • • • • Beans = electrons Blue balls = carbon atoms Yellow balls = phosphate Red ovals = ADP Green rectangles = NAD+ Fig. 6-7b ATP made in Glycolysis is by the process of substrate level phosphorylation Enzyme Enzyme P ADP + P Substrate P Product ATP What’s next? • Pyruvate (pyruvic acid) goes into the mitochondrial matrix – Here it gets converted into acetyl-CoA • This will enter the Krebs (citric acid) cycle • Krebs cycle – Our sugar continues to be broken down releasing Carbon dioxide – ATP (1 per pyruvate) is made – NADH and FADH2 are made • These carry electrons to the electron transport chain Modeling the Krebs cycle • • • • • • • Beans = electrons Blue balls = carbon atoms 7 Yellow balls = phosphate 1 Red ovals = ADP 1 Green rectangles = NAD+ 4 Green hearts = FAD 1 Red square = Coenzyme A (CoA) 1 Oxidative phosphorylation electron transport chain The electron carriers (NADH and FADH2) take the electrons to the electron transport chain located on the inner membrane Energy from the electrons moving through the chain provide the power to make ATP About 34 ATP are made • NADH and FADH2 give electrons at the beginning of the chain • Oxygen takes electrons away at the end – Forming water Modeling oxidative phosphorylation (the electron transport chain) • • • • • • • • • H+ = small green balls Oxygen = medium blue balls Electrons = beans proteins of the electron transport chain = large red ATP synthase = Large blue ADP = red oval Phosphate = yellow ball NADH = green rectangle with electrons FADH 2 = green heart with electrons Aerobic Respiration This process occurs in the mitochondria