Download Data/hora: 06/05/2017 09:47:53 Biblioteca(s): Embrapa

Data/hora: 08/06/2017 17:09:43
Biblioteca(s): Embrapa Agrobiologia.
Data corrente: 01/12/1997
Data da última atualização: 01/12/1997
Autoria: COSTA LIMA, M.A.; VILAR, C.V.; REIS, V.M.; OLIVARES, F.L.; BALDANI, J.I.;
FERREIRA, P.C.G.; HEMERLY, A.S.
Título: Detection of sugarcane (Saccharum officinarum L.) genes induced by endophytic
nitrogen-fixing bacteria.
Ano de publicação: 1996
Fonte/Imprenta: In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E
BIOLOGIA MOLECULAR, 25., maio 1996, Caxambu. Resumos... Caxambu: Sociedade
Brasileira de Bioquimica e Biologia Molecular, 1996. p.42. Resumo E-74.
Idioma: Inglês
Conteúdo: Nitrogen is an essential element for all living organisms,beig used for protcin and nucleic
acids biosynthesis. it is assimilated by plants and animals as ammoniom(NH4) or nitrate
(NO3).atmospheric nitrogen(N2)despite its abundance, cannot be utilized
directly.biologic nitrogen fixation (BNF) is the process by wich atmosphcric nitrogen
becone available and consiste basically in nitrogen reduction,in process caralyzed by
enzymes. only a few species of bacteria and algae are able to do this process. endophytic
nitrongen-fixing bacteria as acetobacter and herbaspirillum were foud in sugarcane
tissues.in this symbiotic association, BNF occurs withont induction of nodule formation,
in a system that differs from rhizobium-leguminoscre interaction. in this system, litle is
known about plant-bacteria interaction of BNF in distinc sugarcane genotypes indicates
that plant genetic factors are contributing to the efficiency of the process. the isolation and
characterization of plant genes induced during the processes of recognition, colonization
and nitrogen fixation by the bacteria wil provid tools for future genetic manipulation to
obtain high rales of BNF. In order to study the role of the plant in this new system of
symbiotic association we have useda modification of the differential disply technique to
detect and isolate cDNAs corresponding to transcripts expressed in sugarcane plants
infected wit Acetobacter diazotrophichs and Herbaspirillum spp..plants were propagated
and infected in vitro. In a preliminary screenig, eight different random primers were
selected to amplify cDNAs present only in infected plants. The differentially expressed
bands obtained were eletud and reaplifiend using the same primers. Southern blot
hybridizations using total cDNA-obtained from infected plants as probe are beig
performed to confim differential expression. The induced genes will be cloned and further
characterized.
Palavras-Chave: Bacteria; BNF; Cana de acucar; Endophytes; FBN; Fixacao biologica de nitrogenio;
Nitrogen fixing bacteria; Parasito de planta; Saccharum officinarum; Sugar cane.
Categoria do assunto: -Registro original: Embrapa Agrobiologia (CNPAB)
Exemplares: CNPAB - (24347-1) - UPC - PL - -- - - - 22188 - DIS