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KS4 Biology B9a / Side 1 Digestion Digestion is the process in which food is broken down and processed in the body. Large insoluble molecules are broken down into small soluble molecules that can pass by diffusion into the bloodstream. These soluble molecules then diffuse into body cells so that important reactions can take place. Digestion takes place by two processes: 1. Mechanical (Physical) The cutting and crushing action of the teeth as feeding takes place achieves physical digestion as well as the rhythmical contractions of the gut and stomach. 2. Chemical This is carried out by acid and biological chemicals called enzymes Feeding – The type of human teeth Activity Using B9a side 2 and mirrors, examine your teeth and record the number of each type of tooth that you possess. Sha Tin College Science Department - June 17 KS4 Biology B9a / Side 2 The Position of Human Teeth The following diagram shows half the lower and upper jaw of a human: Using different coloured pencils for each tooth type colour the diagram. Using the mirror, count the number of each type of tooth that you posses and record your results in the following table. Type of tooth Number Incisor Canine Pre-molar Molar (including wisdom teeth) Total number of adult human teeth: Reasons that this number may not be actually present: Sha Tin College Science Department - June 17 KS4 Biology B9b / Side 1 The Types of Human Teeth Complete the following table: Diagram Appearance (shape) Function INCISOR CANINE PRE-MOLAR MOLAR Sha Tin College Science Department - June 17 KS4 Biology B9c / Side 1 Structure of a Human Tooth Using the table provided, label the following diagram Number Structure Function Pulp Cavity Central space containing nerves and blood supply Fibres Combine with cement to anchor root to jaw Crown Part of tooth above the gum Nerve Sensory endings that respond to join and hot and cold Enamel Extremely hard material forming sharp points and edges – dead material Cement Helps attach root to jaw and acts as a shock absorber Root Part of tooth that lies below the gum Blood vessels Supply food and oxygen to the living cells of the tooth Gum Soft tissue surrounding the bone Jaw Bone that provides the support for the root of the tooth Dentine Hard, bone-like material containing living cells Sha Tin College Science Department - June 17 KS4 Biology B9c / Side 2 Structure of a Human Tooth Complete the following paragraph below using the words provided. Each word may be used more than once or not at all. Food Points 32 Canines Molars Enamel Non-living Diet Snipping Swallowing Hard Bone Oxygen Nerve Pulp Fibres Teeth are designed for breaking down _______________. _______________ teeth. Cells Edges Front An adult human has Teeth are covered in _______________ which is extremely _______________ and _______________ - _______________. It forms sharp _______________ and _______________. Beneath it lies the dentine and which is _______________. hard and These _______________ are supplied with -like contains living _______________ _______________ by blood vessels in the _______________ cavity. and Incisor teeth are found at the _______________ of the mouth and are used for cropping and _______________ at food. The 3 other kinds of teeth found in mammals are _______________, premolars and _______________. Sha Tin College Science Department - June 17 KS4 Biology B9d / Side 1 Tooth Decay Activity Take a portion of tablet and let it dissolve in your mouth for a few minutes. Using the mirrors provided, look at your teeth. What has happened? Reference: Nelson P.143 Applin P.201 What is tooth decay caused by? What is the substance formed on the surface of teeth called? How is this substance formed? What would happen if you did not clean your teeth? What type of foods are bad for your teeth and why? Sha Tin College Science Department - June 17 KS4 Biology B9e / Side 1 The Digestive System The human gut, sometimes called the Alimentary Canal is a long coiled tube, which is 7-9m long. At one end food enters through the mouth (ingested). At the other end the undigested remains of a meal are removed through the anus (egested). Digestion and absorption take place in between. Reference: Nelson P.136 Applin P.204 Label the following diagram of the Alimentary Canal Sha Tin College Science Department - June 17 KS4 Biology B9e / Side 2 Digestion Once food has been taken into the alimentary canal (ingestion) it needs to be broken down into small soluble compounds that are able to be absorbed through the walls of the intestines (mainly the small intestine) into the bloodstream where it can then be used by the body. The 3 main food groups that need to be digested are: Starch (A large insoluble carbohydrate) Proteins Fats Complete the following table: Type of molecule Broken down into Glucose Elements present Food types CHO Margarine, oil, butter CHON What molecules are absorbed into the bloodstream? Sha Tin College Science Department - June 17 KS4 Biology B9f / Side 1 Peristalsis – Moving food through the gut The gut wall is made up of muscle layers that contract and relax thus enabling food to move through the gut. This muscular action is called Peristalsis. How does fibre in the diet help peristalsis to happen? Why can humans not digest fibre? Sha Tin College Science Department - June 17 KS4 Biology B9f / Side 2 Digestive Enzymes The chemical process of digestion involves many different enzymes found at different sites in the human gut. Enzymes: Are biological catalysts Speed up chemical reactions without being used up Made of protein Non-living Substrate specific. They have a special shaped groove that allows them to bind with one specific substrate molecule. This groove is called the active site of the enzyme. (Lock and Key Hypothesis) The shape of the enzyme will change with a change of temperature or pH. If the change in pH is extreme or the temperature rises above the optimum working temperature for that enzyme the enzyme will become denatured (change shape so much that it can not fit with the substrate) and its activity will be destroyed. Activity This experiment demonstrates that digestion is required to break down molecules so that they are small enough to pass through into the bloodstream. Amylase is an enzyme, which breaks down starch into smaller glucose molecules. Where is amylase found in the body? Set up the following experiment using Visking Tubing. This mimics the gut by allowing small soluble molecules to pass through by diffusion. Sha Tin College Science Department - June 17 KS4 Biology B9f / Side 3 1. Set up the Visking Tubing bags as shown in the above diagram. Your teacher will demonstrate this. 2. Fill the labelled bags with the following solutions and put in a 37C water bath for 15 minutes. 3. Using a pipette, take 2 samples from the water in each boiling tube. Test one sample for starch and the other for sugar. Record your results in this table: Test-tube No Solution 1 Starch 2 Glucose 3 Starch + Amylase 4 Amylase Starch test Sugar test What does the water surrounding the visking tubing represent? Sha Tin College Science Department - June 17 KS4 Biology B9f / Side 4 What is the purpose of test-tube 4? Why does the body need to digest starch? Why was warm water used to cover the pieces of visking tubing? Why is it best to use only enough water to just cover the pieces of visking tubing? Sha Tin College Science Department - June 17 KS4 Biology B9g / Side 1 Digestive Enzymes These can be classified under three broad headings: Reference: Nelson P.138 Applin P.203 Complete the following table: Enzyme Reaction speeded up Site of production Site of action Amylase Protease Lipase Sha Tin College Science Department - June 17 KS4 Biology B9g / Side 2 Factors Which Affect Enzyme-Controlled Reactions The rate of an enzyme-controlled reaction is determined by some important factors. Temperature pH This is the reason that enzymes are found working at different locations in the body. Temperature Explain what is happening in Part A of the graph? Sha Tin College Science Department - June 17 KS4 Biology B9g / Side 3 What is the optimum temperature for this enzyme? What does this mean? What happens to the rate of the reaction as temperature continues to increase after the optimum temperature? pH Most enzymes have an optimum pH of around 7, which is the pH found within most cells. However, there are exceptions. Where in the body would you expect to find an enzyme, which has a pH optimum of 1-2? Explain why. Give an example of this type of enzyme. What would happen if the pH of a cell suddenly changed? Try to think of an example, which would cause the pH of the cell to change. Sha Tin College Science Department - June 17 KS4 Biology B9h / Side 1 Activity: The Effect of Temperature on the Enzyme Amylase Amylase Starch --------------------------- Maltose 1. Water baths are set up at the following temperatures 37C 55C 75C Room temperature – desktop 2. For each temperature, label 2 test tubes, starch and enzyme. Measure 2ml of each solution and place into the correct tubes. 3. Place the labelled tubes into the appropriate water bath. 4. Leave for 5 minutes. 5. Pour enzyme solution into the starch test tube and stir. Start a stopwatch. 6. Every minute from then on put on one-drop of the mixture on to a dropping tile using a clean glass rod. Test with iodine. 7. Repeat for each temperature. Results Produce a table of results and plot a graph. Then answer the following questions. Sha Tin College Science Department - June 17 KS4 Biology B9h / Side 2 Why is the temperature so important? Why is it important to keep the solutions separate at first? At what temperature is there the faster breakdown of the starch solution? Is this what you would expect? What has happened to the enzyme when the temperature is too hot? What would happen if you changed the pH of the solutions? Sha Tin College Science Department - June 17 KS4 Biology B9i / Side 1 Absorption of Food – Small Intestine Food has to be absorbed into the bloodstream so that the body can make use of it. This takes place in the small intestine. The small intestine possesses many features that ensure that this process is efficient. Reference: Nelson P.139, Applin P.206. Complete the table below: Feature Reason Approximately 5m long Very narrow Inner surface is folded into villi projections Each villi is covered with microvilli The cell lining is only 1 cell thick Well supplied with blood vessels Lacteal present Sha Tin College Science Department - June 17 KS4 Biology B9i / Side 2 Answer the following questions. 1. Which blood vessel does the absorbed food pass into? 2. Where does this vessel take the blood to in the body? 3. What is the process called that allows the passage of food through the wall of the intestine? 4. How would you describe the food at this stage of digestion? 5. What is the purpose of the Lacteal? 6. Why is the Lacteal important? 7. The lining of the gut is selectively permeable. What does this mean and why is this important for absorption? 8. Why do vitamins and minerals not need to be digested before they are absorbed? Sha Tin College Science Department - June 17 KS4 Biology B9i / Side 3 Egestion By the time food reaches the rectum most of the food substance have been absorbed into the blood. All that remains is undigestable food (fibre), bacteria and some dead cells from the lining of the alimentary canal wall. This mixture forms the faeces, which are egested through the anus. Complete the following table. (Remember that some of the process occur in more that one place along the gut.) Process Where does it happen in the gut Digestion Absorption of digested food molecules Absorption of water Egestion What is the difference between excretion and egestion? Give 3 examples of excretion Sha Tin College Science Department - June 17