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DNA Fingerprinting Lab Answer Sheet Names ____________________________________________________ 1. Who 1st developed the process of DNA fingerprinting? __________________________ 2. When was the process developed? _______________ 3. What country was the 1st to use DNA fingerprinting in court? In what year? _______________________ 4. What are nonsense codes? ______________________________________________________________ ______________________________________________________________________________________ 5. How do nonsense codes compare in different individuals? _____________________________________ _______________________________________________________________________________________ 6. What are core sequences? _______________________________________________________________ _______________________________________________________________________________________ 7. What did Jeffery’s use for a genetic marker? ________________________________________________ 8. What is the function of restriction enzymes? ________________________________________________ Each of the following statements is a step in the process of DNA fingerprinting; write the number of the step being describe. Step _____ a. DNA band pattern is transferred to nylon membrane. Step _____ b. Radio active DNA pattern transferred to x-ray film. Step _____ c. Blood or cell sample from which DNA is extracted. Step _____ d. DNA probe binds to specific DNA sequence. Step _____ e. The resultant visible pattern is the DNA fingerprint Step _____ f. Restriction enzyme used to cut DNA into fragments Step _____ g. Extra probe material is washed away, leaving unique DNA band pattern. Step _____ h. Fragments are separated into band using electrophoresis. Part II The Paternity Suit Case 1 Was the woman’s claim justified? ___________________________________________________ Explain ________________________________________________________________________ Case 2 On the basis of the evidence is the woman’s claim true? _________________________________ Explain ________________________________________________________________________ Part IV DNA in the Courtroom Case 3 1. Who was the murderer? _________________________ 2. Explain how you determined the murderer ____________________________________________ 3. Should suspects have to provide a blood sample if requested by police? (more than just a yes or no answer) _________________________________________________________________________________________ 4. Would you consider it an invasion of your rights? (more than just a yes or no answer) _________________ _________________________________________________________________________________________ Case #4 Could the man be the father of this child? _____________________ How do you know? ____________________________________________________________________