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Register or Login: Quick Search: withi n user name Passwor d: This Volume/Issue 3 of 13 Infection, Genetics and Evolution Volume 2, Issue 2 , December 2002, Pages 107-110 This Document Abstract Full Text + Links PDF (60 K) Actions E-mail Article doi:10.1016/S1567-1348(02)00090-4 Copyright © 2002 Elsevier Science B.V. All rights reserved. Detection of Salmonella typhi by polymerase chain reaction: Implications in diagnosis of typhoid fever Ashwani Kumar a , a, Vineet Aroraa, Anu Bashamboob and Sher Alib University College of Medical Sciences, GTB Hospital, Shahdara, Delhi 110095, India b National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India Received 22 March 2002; revised 9 July 2002; accepted 18 July 2002. Available online 12 September 2002. Abstract The present study was conducted to detect Salmonella typhi by polymerase chain reaction (PCR) in a clinical setting. A group of 40 clinically suspected cases of typhoid fever, lasting for about 3–11 days, with or without chills and rigors and hepatosplenomegaly were selected. Of these, 20 were culture positive and the remaining 20 were found to be negative by conventional blood culture technique. Primary PCR was followed by nested PCR using two sets of primers corresponding to flagellar gene of S. typhi strain. Two bands of about 458 and 343 bp were detected in 20 blood culture positive cases and 12 of the 20 culture negative ones. In the simulated group of samples, no amplification was detected. Our results suggest that PCR-based diagnosis is particularly useful for all clinically suspected cases of typhoid fever. The sensitivity of PCR and its potential use in routine diagnosis and epidemiological studies of typhoid fever can be exploited to complement studies by including bone marrow culture, faeces and bile samples. Author Keywords: DNA diagnosis; Salmonella typhi infection; Typhoid fever; Septicemia; Nested PCR Corresponding author. Tel.: +91-11-228-2971; fax: +91-11-228-2106. Infection, Genetics and Evolution Volume 2, Issue 2 , December 2002, Pages 107-110 This Document Abstract Full Text + Links PDF (60 K) Actions E-mail Article 3 of 13 Feedback | Terms & Conditions | Privacy Policy Copyright © 2005 Elsevier B.V. All rights reserved. ScienceDirect® is a registered trademark of Elsevier B.V. Register or Login: Quick Search: withi n user name Password: This Volume/Issue 3 of 13 Infection, Genetics and Evolution Volume 2, Issue 2 , December 2002, Pages 107-110 This Document Abstract Full Text + Links PDF (60 K) Actions E-mail Article doi:10.1016/S1567-1348(02)00090-4 Copyright © 2002 Elsevier Science B.V. All rights reserved. Detection of Salmonella typhi by polymerase chain reaction: Implications in diagnosis of typhoid fever Ashwani Kumar , a, Vineet Aroraa, Anu Bashamboob and Sher Alib a University College of Medical Sciences, GTB Hospital, Shahdara, Delhi 110095, India b National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India Received 22 March 2002; revised 9 July 2002; accepted 18 July 2002. Available online 12 September 2002. Abstract The present study was conducted to detect Salmonella typhi by polymerase chain reaction (PCR) in a clinical setting. A group of 40 clinically suspected cases of typhoid fever, lasting for about 3–11 days, with or without chills and rigors and hepatosplenomegaly were selected. Of these, 20 were culture positive and the remaining 20 were found to be negative by conventional blood culture technique. Primary PCR was followed by nested PCR using two sets of primers corresponding to flagellar gene of S. typhi strain. Two bands of about 458 and 343 bp were detected in 20 blood culture positive cases and 12 of the 20 culture negative ones. In the simulated group of samples, no amplification was detected. Our results suggest that PCR-based diagnosis is particularly useful for all clinically suspected cases of typhoid fever. The sensitivity of PCR and its potential use in routine diagnosis and epidemiological studies of typhoid fever can be exploited to complement studies by including bone marrow culture, faeces and bile samples. Author Keywords: DNA diagnosis; Salmonella typhi infection; Typhoid fever; Septicemia; Nested PCR Corresponding author. Tel.: +91-11-228-2971; fax: +91-11-228-2106. This Document Abstract Full Text + Links PDF (60 K) Actions E-mail Article Infection, Genetics and Evolution Volume 2, Issue 2 , December 2002, Pages 107-110 3 of 13 Feedback | Terms & Conditions | Privacy Policy Copyright © 2005 Elsevier B.V. All rights reserved. ScienceDirect® is a registered trademark of Elsevier B.V. Register or Login: Quick Search: withi n user name Passwor d: This Volume/Issue 5 of 12 Infection, Genetics and Evolution Volume 2, Issue 1 , October 2002, Pages 39-45 This Document Abstract Full Text + Links PDF (87 K) Actions E-mail Article doi:10.1016/S1567-1348(02)00089-8 Copyright © 2002 Elsevier Science B.V. All rights reserved. Salmonella typhi, the causative agent of typhoid fever, is approximately 50,000 years old*1 Claire Kidgella, Ulrike Reichardb, John Waina, Bodo Linzb, Mia Torpdahlc, Gordon Dougana and Mark Achtman , , b a Centre for Molecular Microbiology and Infection, Imperial College of Science, Technology and Medicine, The Flowers Building, Exhibition Road, South Kensington, London SW7 2AY, UK b Department of Molecular Biology, Max-Planck Institut für Infektionsbiologie, Schumannstrasse 21/22, 10117, Berlin, Germany c Danish Veterinary Laboratory, Bülowsvej 27 1790, Copenhagen, Denmark Received 11 March 2002; revised 22 April 2002; accepted 23 April 2002. Available online 27 September 2002. Abstract A global collection of 26 isolates of Salmonella typhi was investigated by sequencing a total of 3336 bp in seven housekeeping genes. Only three polymorphic sites were found and the isolates fell into four sequence types. These results show that S. typhi is a recent clone whose last common ancestor existed so recently that multiple mutations have not yet accumulated. Based on molecular clock rates for the accumulation of synonymous polymorphisms, we estimate that the last common ancestor of S. typhi existed 15,000–150,000 years ago, during the human hunter-gatherer phase and prior to the development of agriculture and the domestication of animals. Author Keywords: Age; Salmonella; Multilocus sequence typing; Housekeeping gene; Sequence diversity; Clone; Epidemic disease; Microbial evolution *1 Nucleotide sequence data reported in this paper are available in the GenBank database AY142218-41. Corresponding author. Tel.: +49-30-28460-751; fax: +49-30-28460-750; email: [email protected] Infection, Genetics and Evolution Volume 2, Issue 1 , October 2002, Pages 3945 This Document Abstract Full Text + Links PDF (87 K) Actions E-mail Article 5 of 12 Feedback | Terms & Conditions | Privacy Policy Copyright © 2005 Elsevier B.V. All rights reserved. ScienceDirect® is a registered trademark of Elsevier B.V.