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Name: ______________________________________ # ______
Magnet Biology
DNA Replication
Directions – Use the link below and go through the DNA replication animation to
answer the following questions. There are 8 sections; be sure to view all of them.
http://www.wiley.com/college/pratt/0471393878/student/animations/dna_replication/
index.html
1) What is DNA Replication?
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2) Explain what the term ‘semi-conservative’ means and why DNA replication is
considered to have this property.
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3) Explain the function of each of the following:
Protein Structure
Function
DNA Helicase
Single Stranded Binding Proteins
Primase
DNA Polymerase
Sliding Clamp
RNase H
DNA Ligase
4) Explain the events that occur during the 1st step of DNA replication (be
specific).
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5) What does it mean to say DNA has 2 anti-parallel strands with
complementary 5’ to 3’ strands running in opposite directions?
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6) In which way can polymerase enzymes synthesize new DNA?
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7) In what direction does DNA synthesis occur?
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8) What is the function of primase? Would DNA polymerase be able to do it’s
job with out primase? Why or why not?
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9) Explain the difference between the discontinuous and continuous strand of
DNA during replication.
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10) Which is known as the leading strand and which is known as the lagging
strand?
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11) What are Okazaki fragments?
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12) Why must Okazaki fragments be joined together AND how does this work?
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13) RNase H aids DNA replication by:
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14) Go through review question 7 and list the steps in DNA replication below:
1) _____________________________________________________________________
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2) _____________________________________________________________________
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3) _____________________________________________________________________
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4) _____________________________________________________________________
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5) _____________________________________________________________________
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6) _____________________________________________________________________
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7) _____________________________________________________________________
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8) _____________________________________________________________________
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HINT: It would be a good idea to bookmark this site so you can use it to study for
your test!