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Name: ______________________________________ # ______ Magnet Biology DNA Replication Directions – Use the link below and go through the DNA replication animation to answer the following questions. There are 8 sections; be sure to view all of them. http://www.wiley.com/college/pratt/0471393878/student/animations/dna_replication/ index.html 1) What is DNA Replication? ________________________________________________________________________ ________________________________________________________________________ 2) Explain what the term ‘semi-conservative’ means and why DNA replication is considered to have this property. ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 3) Explain the function of each of the following: Protein Structure Function DNA Helicase Single Stranded Binding Proteins Primase DNA Polymerase Sliding Clamp RNase H DNA Ligase 4) Explain the events that occur during the 1st step of DNA replication (be specific). ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 5) What does it mean to say DNA has 2 anti-parallel strands with complementary 5’ to 3’ strands running in opposite directions? ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 6) In which way can polymerase enzymes synthesize new DNA? ________________________________________________________________________ ________________________________________________________________________ 7) In what direction does DNA synthesis occur? ________________________________________________________________________ ________________________________________________________________________ 8) What is the function of primase? Would DNA polymerase be able to do it’s job with out primase? Why or why not? ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 9) Explain the difference between the discontinuous and continuous strand of DNA during replication. ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 10) Which is known as the leading strand and which is known as the lagging strand? ________________________________________________________________________ ________________________________________________________________________ 11) What are Okazaki fragments? ________________________________________________________________________ ________________________________________________________________________ 12) Why must Okazaki fragments be joined together AND how does this work? ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 13) RNase H aids DNA replication by: ________________________________________________________________________ ________________________________________________________________________ 14) Go through review question 7 and list the steps in DNA replication below: 1) _____________________________________________________________________ _____________________________________________________________________ 2) _____________________________________________________________________ _____________________________________________________________________ 3) _____________________________________________________________________ _____________________________________________________________________ 4) _____________________________________________________________________ _____________________________________________________________________ 5) _____________________________________________________________________ _____________________________________________________________________ 6) _____________________________________________________________________ _____________________________________________________________________ 7) _____________________________________________________________________ _____________________________________________________________________ 8) _____________________________________________________________________ _____________________________________________________________________ HINT: It would be a good idea to bookmark this site so you can use it to study for your test!