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BIRLA INSTITUTE OF TECHNOLOGY AND SCIENCE, PILANI
INSTRUCTION DIVISION
Course Handout (Part II)
In addition to part-I (General Handout for all courses appended to the timetable) this
portion gives further specific details regarding the course.
Course No.
Course Title
: BIO G512
: Molecular Mechanism of Gene Expression
1. Course Description:
Prokaryotic and Eukaryotic genomes and their topology; DNA protein interaction, RNA
transcription
and
transcriptional
control,
Translation,
RNA
processing,
Posttranscriptional control and other mechanisms of gene expression
2. Scope & Objective:
This course is designed to impart knowledge of molecular genetics, which is one of the
fundamental requirements for the biotechnology. This course is designed to make the
student understand the various mechanisms, which regulate the biological processes at
genetic level including transcriptional, and post transcriptional regulation of gene.
.
3. Text Books;
T1: Gene VII: by Benjamin Lewin; Oxford New York, Tokyo, 2000
4. Course Plan :
Lecture
No
Learning Objective
Topics to be covered
1
Genes are DNA
2.
3.
4.
5.
Overview of molecular
Genetics
--do---do---do-Prokaryotic Transcription
6.
-do--
7.
8.
9.
10
-do-Prokaryotic Translation
-do--do--
From Genes to Genome
How many gene are there
Clusters and repeats
Structure and role of RNA
Polymerase
Promoter recognition and
binding
Role of sigma and rho factor
t-RNA
Translation in Prokaryotes
Translation in Eukaryotes
1
Reference*
Chap/Sec
(Books)
Chap.1
Chap.2
Chap.3
Chap.4
Chap.9
--do---do-Chap.5
--do---do--
11
Initiation
Chap.6
12
13
Prokaryotic Protein
Synthesis
-do--do--
Elongation
Role of ribosomes and r-RNA
--do---do--
14
Use of genetic code
Structure and wobbling in
codons, anticodones
Mitochondrial and archetypal
code, mutated anticodones
Accuracy of translation
Protein folding and protein
insertion into membrane and cell
organelles
How do protein enter and leave
membranes
Role of nuclear pore,
degradation of proteins
Structural gene clusters,
Repressor
Operator and regulator gene,
Specificity of protein-DNA
interaction
Positive and negative control,
Catabolite repression
Stringent response,Autogenous
control,
Attenuation and regulation by
small RNA molecules
Control of lytic cascades in T4,
T7 and lambda phage,
Maintenance of lysogeny
Repressor synthesis and binding
Chap.7
15
16
17
-do--do-Protein Localization
18
-do--
19
-do--
20
The Operon concept
21
-do--
22
-do--
23
-do--
24
-do--
25
Phage Strategies
26
-do--
27
-do--
28
Eukaryotic Transcription
29
-do--
30
-do--
31
Regulation of
transcription in
eukaryotes
Balance between lysogeny and
lysis
Initiation of transcription;
Eukaryotic RNA Polymerase-I
& III
Eukaryotic RNA Polymerase- II,
Connection between trancription
and repair
Promoter & Enhancers,
Transcription factors
Response elements and DNA
binding domains; Zinc finger
motifs, steroid receptors
2
--do---do-Chap.8
--do---do-Chap.10
--do--
-do--do--do-Chap.11
-do—
-do-Chap.20
-do--
-do-Chap.21
32
-do--
33
-do--
34
35
36
Post Transcriptional
modification
--do--
Catalytic RNA
37
--do--
38
Immune Diversity
39
--do--
40
--do--
Topics for Self Study &
Broad area of Seminar
Topic
Homeodomains,Helix-turn helix,
Leucine zippers, Chromatin
modeling
Histone acetylation and
deacetylation control,
demethylation and gene
expression
Nuclear Splicing; Splice
junctions, lariate formation and
spliceosomes
Splicing of group II introns,
Alternative splicing, Yeast tRNA splicing
Role of catalytic RNA in Group
I intron removal ribozyme,
Ribonuclease acivities and RNA
editing
Clonal selection,Assembly of
immunoglobulin gene in
lymphocytes Diversity of
germline
,Recombination between V & C
segments, Allelic exclusion,
Class switching, Somatic
mutations
, B-cell development and
Memory, T-cell receptors, Major
histocompatibility locus
Transposons
Retroviruses and Retroposones
Protein Trafficking
Signal Transduction
Cell Cycle and Growth
Regulation
Oncogenes and Cancer
5. Examination Scheme:
3
-do--
-do--
Chap.22
--do--
Chap.23
--do-Chap.24
--do--
--do--
Chap. 15
Chap.16
Chap. 25
Chap.26
Chap.27
Chap.28
EC
No
Evaluation
Component
1
2
3.
4.
5.
Test-1
Test-II
Seminar
Assignment
Comprehensive
Examination
Duration
Date, Time
50 min
50 min
20 min
20 min
3 Hrs.
& Venue
Weitage(%)
20
20
10
10
40
Remarks
CB
OB
CB
6. Chamber Consultation Hour: To be announced
7. Notices; Notices will be displayed on Biological Science Notice Board
8. Make-up Policy: Prior Permission has to be obtained from the Instructor-In-Charge
for make-ups.
Instructor-in-charge
BIO G512
4