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CELL DIVISION AP LAB PROTOCOL Part A – The Eukaryotic Cell Cycle 1. 2. Fold a piece of computer paper in half, then into thirds creating six sections. Using landscape orientation, in the top left section record the … Title Organism Represented Magnification Power Used Your Name 3. Title each of the remaining sections with the phases of the cell cycle in order, beginning with interphase. 4. Obtain a Whitefish Blastula slide. Scan several fields on low power. 5. Switch to high power and locate a representative cell for each phase of the cell cycle. Draw, color, and label the cell according to the following guidelines: All drawings must be in pencil. No pens or markers, please Use colored pencils for coloring and shading, always according to true color. Drawings should be large enough to see details. Please use at least ½ of the allotted space. All labeling is done to the right of the drawing with a ruler. 6. Label the following structures, if present and visible Chromatin Chromosomes – we are using this term to describe DNA in coiled, condensed form Please indicate in which phases the chromosomes consist of sister chromatids Nuclear envelope Nucleolus Cell membrane Cleavage furrow Spindle Fibers Centrosome Please note: You may work as a table to locate the different phases of the cell cycle; however, all drawings must be done from the microscope, not another student’s paper. This paper must be turned in at the end of each class period. Part B – Timing of the Cell Cycle in Onion Root Tips 1. 2. 3. 4. 5. 6. 7. Construct a Data Table in your Lab Notebook. Obtain an Onion (Allum) Root Tip slide. Locate the region of the root tip where there is meristem tissue and actively-dividing cells. Count 100 cells, following an organized, sequential pattern and identify them as representing interphase or mitosis. Have your partner keep a tally for you. Now switch places with your partner(s). Make sure each counter examines a different root tip. Tally your table data. Find the table means/100 cells. Record on the white board. Calculate the class mean for interphase versus mitosis. Meristem Part C – A Comparison of Mitosis and Meiosis 1. 2. Use the chromosome set provided to work through the cell cycle and meiosis with your partner. Please use the following information: You have materials to create a cell in which 2n = 6. Each strand of beads represents a molecule of DNA. The two different colors of beads represent maternal versus paternal genetic information. Like-chromosomes are represented by like-number of beads. The middle magnets represent centromeres. Each clear tube represents a centrosome containing one pair of centrioles. o Consider the orientation of the DNA/spindle fibers when placing the centrosome(s). o Consider whether the centrosome is located inside or outside of the nuclear envelope. The string represents the nuclear envelope. Mini-practical … without notes, of course! You will be asked to put your cell in three different phases of the cell cycle/meiosis. There is no partial credit for each phase.