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Dipartimento di Scienze e Tecnologie Chimiche e dei Biosistemi
(DSTCB)
Time Determination of Metabolic Modules in Saccharomyces cerevisae
Contact person details
Name:
RICCI
Name of
Organisation:
Dipartimento di Scienze e Tecnologie Chimiche e dei Biosistemi
(DSTCB)
URL:
E-mail:
Contact
Telephone:
+39-0577-234367
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Collaboration
Type: Project Proposal
Title: Time Determination of Metabolic Modules in Saccharomyces cerevisae
Participation: Type A
Type Details: During the past years we assisted in many improvements in the
metabo(l/n)omic research
which shifted biology from a reductionism paradigm (the observation of a limited set
of
metabolic reactions) to systems biology paradigm which considers the cell as the
whole
organism. Mathematical models based on the results of this research have been
proposed to identify modules which are, by definition, a discrete entity whose
function is separable from those of other modules. In order to understand the
dynamic processes behind the modules behavior we need to combine
phenomenological analysis with molecular studies.
The aim of this proposal is to correlate the time course evolution of the metabolites
with the expressed during the fermentative process in Saccharomyces cerevisiae.
The description of cellular networks and modules requires experimental techniques
able to
generate and interpret large time-series data. We plan to collect two kind of data
during
fermentative metabolism of yeast: metabolic profiles and genes expression levels.
Timecourse metabolic profiles will be performed using NMR on both, cell extract
(footprint) and cell growth medium (fingerprint); the experimental platform for
obtaining
time-courses of gene expression levels will be provided by microarray technology.
This kind of approach will imply, beside the acquisition of large amounts of data, the
challenging exploration and interpretation of the data themselves.
We will adopt Principal Component Analysis (PCA) in order to highlight which
changes in
metabolic profile -during the fermentative process- are related with changes at gene
expression level, subsequently we aim to derive a mathematical interpretation of this
dynamic behavior able to generate hypothesis regarding the modular organization of
metabolite and the correlated pool of genes.
Programme: FP7-HEALTH
Instruments: CP-FP Small or medium-scale focused research project
Research interest:
Expiry Date: 2008-12-04
Workprogramme area(s)/key action(s):
Target Partner
Expertise:
we are looking for scientist with experience in yeast or microorganisms metabolism
and scientist interested in mathematical models of life processes.
Country:
World
Organization Details
Name:
Dipartimento di Scienze e Tecnologie Chimiche e dei Biosistemi
(DSTCB)
Department:
Dipartimento di Scienze e Tecnologie Chimiche e dei Biosistemi
Address:
SIENA
ITALIA
Type:
Research, Education
Number of
Employees:
10 - 49
Details:
Keywords:
Partners already
acquired:
Yeast
NMR
Metabolism
microarray
mathematical model
module
fermentative process