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Topic Histology of liver And Gall bladder
Learning objectives:
At the end of the practicle student will able:
To identify the classical lobule of liver.
To explain the arrangement of hepatocytes and sinusoidal space of liver.
To give the different arrangement of hepatic lobules.
To give the normal histology of gall bladder.
 It is the largest gland of body.
 Covered by connective tissue capsule.
 Parenchyma consists of hepatocytes.
 Histologically liver consists of innumerable pyramidal lobule
 Lobules consists of hepatocytes.
 They are located in flat irregular plates that are arranged radially like
the spokes of a wheel around a branch of the hepatic vein, called the
central vein or central venule since it really has the structure of a
 Between these plates of hepatocytes are blood spaces known as liver
 Liver
(medium magnification)
 Section of Liver (in two dimension)
 Hepatocytes are arranged in rows that radiate out from the central
 These rows are one cell wide and are surrounded by sinusoidal
capillaries or sinusoids.
 This arrangement ensures that each hepatocyte is in very close contact
with blood flowing through the sinusoids, i.e. bathed in blood.
 Hepatocytes
 They are large cells.
 Nucleus is large and rounded with prominent nucleolus
 25% cells are binucleated.
 Cytoplasm is strongly eosinophilic.
 There is fine basophilic granularity due to the presence of free
 Lipofuscin granules are also present in cytoplasm and increase with
 The sinusoidal lining cells are endothelial cells, kupffer cells and cells
of The endothelial cells lining sinusoids are fenestrated and in most
species lack a basal lamina
 A narrow space is present between the surface of the hepatocyte and
the surface of the endothelial cell.
This is called the space of Disse; it is filled with numerous microvilli
from the hepatocytes
 The Kuffer cells are found on the luminal surface of endothelial cells.
 These are phagocytic cells that remove particulate material and old
red blood cells from circulation.
 Kupffer cells are members of the mononuclear phagocyte system.
Basic Functional Unit of The Liver
1) Classical hepatic lobule.
2) Portal acinus
3) Hepatic acinus.
Classical hepatic lobule:
 The hepatic lobule is defined as having a central vein (CV) at its
center with hepatocytes radiating from it in the form of plates.
 The classical hepatic lobule is hexagonal in shape
 Terminal branches of hepatic artery, portal vein and bile ductule are
located at the angels of hepatic lobule boundary, known as portal
 Classical Hepatic Lobule
 Portal Lobules
 Portal lobule
 This lobule is triangular in shape.
 Having a triad in the center and three hepatic veins at the corners.
 The Hepatic Acinus
 It is the functional unit of liver, comprises the territory supplied by
each terminal branch of hepatic artery and portal vein and
accompanied by bile ductule.
 It is diamond shape.
 It is situated in adjacent area of two classical hepatic lobules.
 Termial branch of hepatic artery and portal vein run transversely in
the centre of acinus.
 Hepatocytes and sinus radiate towards hepatic venule
The hepatic acinus has three zones.
a) Hepatocytes in Zone 1 are the first to receive blood and it is high in
b) Hepatocytes in Zone 2 are the second cells to receive blood and it is
lower in oxygen.
c) Hepatocytes in Zone 3 are the last to receive blood from a branch of
the hepatic artery and it is lowest in oxygen.
 Hepatic Acinus (Zones)
 Thus, the cells with the highest metabolic potential are found in Zone
 Those with the least are found in Zone 3.
 Importantly, the cells in Zone 3 are the most susceptible to ischemic
conditions as edges defined by portal canals (PC).
 Duct System of Liver
 Bile is produced and secreted by hepatocytes into a special "duct"
called a bile canaliculus.
 This "duct" is actually just a space formed between two hepatocytes
that is separated from the connective tissue space around the
hepatocytes by the presence of tight junctions.
 The bile canaliculi empty into branches of the bile ductules.
 In the duct system, bile flows in the direction opposite to the flow of
blood in the sinusoids.
 Blood Supply of Liver
 Functions of Liver
Digestive and Metabolic Functions
 Synthesis and secretion of bile
 Storage of glycogen and lipid reserves
 Maintaining normal blood glucose, amino acid and fatty acid
 Synthesis and release of cholesterol bound to transport proteins
 Inactivation of toxins
 Storage of iron reserves
 Storage of fat-soluble vitamins
Non-Digestive Functions
• Synthesis of plasma proteins
• Synthesis of clotting factors
• Synthesis of the inactive angiotensinogen
• Phagocytosis of damaged red blood cells
• Storage of blood
• Breakdown of circulating hormones (insulin and epinephrine) and
• Inactivation of lipid-soluble drugs