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Biology EOC Review #3 North Carolina Test of Biology 4. _____ 21. _____ name: _____________ 45. _____ Test 1: 34. _____ Test 2: 11. _____ 5. _____ 25. _____ 50. _____ 41. _____ 14. _____ 7. _____ 28. _____ 53. _____ 50. _____ 15. _____ & 26. _____ Purple Book: Biology EOC Biology Sample Items Goal 2____________________________________________________________ 10. ______ 11. ______ 12. _____ EOC Biology Sample Items Goal 3 13. _____ _________________________ 1. _____ 6. _____ 11. _____ 16. ____ 21. ____ 2. _____ 7. _____ 12. _____ 17. ____ 22. ____ 3. _____ 8. _____ 13. _____ 18. ____ 23. ____ 4. _____ 9. _____ 14. _____ 19. ____ 5. _____ 10. _____ 15. _____ 20. ____ 2.03 Investigate and analyze the cell as a living system including: maintenance of homeostasis, movement of materials into and out of cells, and energy use and release in biochemical reactions. Energy, Photosynthesis, and Cellular Respiration ATP: adenosine triphospate – usable form of energy for living organisms ADP: adenosine diphosphate – possesses no energy ATP ADP + P reaction showing the release of energy (energy is stored in the third phosphate) ADP + P ATP reaction showing the capture or gain of energy Photosynthesis o Definition: absorption of sunlight energy to make glucose (food) o Organisms that use photosynthesis: plants, phytoplankton, and bacteria o Summative formula for photosynthesis: Energy + carbon dioxide (CO2) + water (H2O) glucose (C6H12O6) + oxygen (O2) o Location: chloroplast o Pigments: absorb sunlight energy; chlorophyll is an example of a pigment o Plants absorb 6-carbon dioxide molecules; they use the carbons (C) to make glucose Cellular respiration o Definition: breakdown of glucose to make energy (ATP) o Organisms that use cellular respiration: every living thing, including plants o Summative formula for cellular respiration: Glucose (C6H12O6) + oxygen (O2) Energy + carbon dioxide (CO2) + water (H2O) o Location: mitochondria o Anaerobic respiration: reaction that occurs without oxygen present; produces little ATP o Fermentation: Breaking down of “leftover” glucose as waste; alcohol and lactic acid fermentation o Aerobic respiration: reaction that occurs with oxygen; produces the MOST ATP Use the following diagram to show where energy is released and where energy is used. Also use arrows on the lines attached to the circles to indicate the direction of the energy. a. How many phosphates? ATP? _________ ADP? ________ b. What is ATP energy used for? Give examples. c. What cellular process produces ATP? Rev #3 1 2.05 Investigate and analyze the bioenergetic reactions: aerobic respiration, anaerobic respiration, and photosynthesis. Section 1: What are the reactants and products for each of these? Process Reactant Product Example? Photosynthesis Cellular Respiration (Aerobic) Cellular Respiration (Anaerobic) Label the following molecules in these equations (water, glucose, oxygen, carbon dioxide, ethyl alcohol) A) B) a. Which of the above reactions is photosynthesis? b. Which of the above reactions is cellular respiration (aerobic)? c. Which reaction(s) requires or stores energy? d. Which reaction requires chlorophyll? e. Which reaction requires light? What is the purpose of the chlorophyll? What is the light used for? f. Which organisms carry out process A? g. Which organisms carry out process B? h. Which process uses chloroplasts in eukaryotes? i. Which process uses mitochondria in eukaryotes? j. What factors could speed up (or slow down) process A? k. What factors could speed up (or slow down) process B? 3.01: Analyze the molecular basis of heredity including: DNA replication, Protein Synthesis (transcription and translation), and gene regulation. Rev #3 2 DNA DNA – genetic make-up of organisms Monomers of DNA are nucleotides DNA nucleotide is made up of three components: sugar (deoxyribose), phosphate, and nitrogen base (adenine, thymine, guanine, and cytosine) Nitrogen bases base pair or combine -Adenine (A) pairs with Thymine (T) and Guanine (G) pairs with Cytosine (C) A=T and G=C Watson and Crick discovered the shape of DNA – it is a double helix which is shaped like a twisted ladder. The handles or sides of the ladder are made up of phosphate and sugar and the steps are the nitrogen bases (A, T, G, C) DNA replication: process in which DNA is copied. Process: (a) the nitrogen bases (steps) are separated (b) DNA polymerase (enzyme) adds complementary nitrogen bases to the exposed sides. Protein synthesis Protein synthesis is the process in which a gene from DNA is “read” and made into a protein. RNA (ribonucleic acid) is the main character in the process of protein synthesis. RNA is different from DNA is three ways: 1) it is single stranded (DNA double stranded) 2) is has ribose (DNA has dexoyribose) 3) it has uracil instead of thymine Three types of RNA: 1) mRNA (messenger RNA): copies message from DNA 2) tRNA (transfer RNA) brings amino acids to mRNA 3) rRNA (ribosomal RNA) found in ribosome and forms peptide bonds between amino acids Two processes in protein synthesis: o Transcription: takes place in the nucleus; mRNA copies the “message” from DNA If DNA read ATG/GGC/AAA/CTT; the complementary mRNA strand UAC/CCG/UUU/GAA o Translation: takes place in the cytoplasm on a ribosome; tRNA brings the matching amino acids to the codons found on mRNA Codon: three base sequence found on mRNA Anticodon: three base sequence found on tRNA To find the matching amino acids, use the chart of mRNA codons that will be provided. The amino acid sequence for the mRNA strand UAC/CCG/UUU/GAA would be as follows: Tyr-ProPhe-Glu Please remember that when using the chart USE MRNA CODONS!!!! Mutation: change in DNA Two types of mutations: 1) Point mutation: when there is an incorrect base within DNA; only one POINT of DNA is affected; for example, DNA sequence ATG and following replication sequence changes to AAG 2) Frameshift mutation: when a base is deleted or inserted into DNA; causes the whole sequence of DNA to change which leads to an incorrect protein; for example DNA sequence is ATG and following replication the sequence is AAT G…..; the A was inserted into DNA Section 2: Rev #3 3 a. Below is a strand of DNA. DNA in the cells exists as a double helix – what else needs to be added to this strand to make it a double helix? Give the nucleotide sequence. b. What are the black pentagons? c. What are the nitrogen bases? d. If the strand of DNA above undergoes transcription, what will the sequence of the mRNA be? e. After translation, what would the amino acid sequence be for this section of mRNA? (read from right to left) f. What is a codon? g. Compare RNA and DNA in the following table RNA DNA Sugars Bases Strands Where In Cell? h. What kind of bond hold the amino acids together in the protein that is formed? i. What are the three types of RNA and what are their functions? j. What kind of weak bonds hold the two strands of DNA together? k. Why is it important that these bonds be weak? l. What happens to DNA when a mutation occurs? m. How does this affect the mRNA? n. How can this affect translation? o. How does this affect the structure and shape of the resulting protein? Rev #3 4 3.02 Compare and contrast the characteristics of asexual and sexual reproduction. Mitosis and Meiosis Diploid (2n) – normal number of chromosomes within an organism; represents homologous pairs (chromosome from mom and dad); the majority of the cells in your body are 2n Haploid (n) – half the number of chromosomes; the only cells in an organism that are n are the gametes or sex cells (sperm and egg) Mitosis produces an identical copies following division Meiosis produces different cells with half the number of chromosomes Mitosis Meiosis Type of reproduction Asexual: one individual makes Sexual: two individuals another Chromosome # of mother cell (mother cell is Diploid (2n) Diploid (2n) original cell before division) Chromosome # of daughter cells (resulting Diploid (2n) Haploid (n) cells after division) # of cell divisions 1 2 # of cells produced 2 4 Section 3: MITOSIS MEIOSIS Type of reproduction (Asexual or sexual) Chromosome number of mother cell (1N=haploid or 2N=diploid) Chromosome number of daughter cells (1N=haploid or 2N=diploid) Number of cell divisions Number of cells produced When does replication happen? SOURCES OF VARIATION Crossing over Random assortment of chromosomes Gene mutations Nondisjunction Fertilization Put the following stages of mitosis (cell division) in order. 1st: _______ called ___________________ 2nd: _______ called ___________________ 3rd: _______ called _____________________ 4th: _______ called _____________________ a. How many chromosomes do humans have in their… Body cells? ________ Sex cells? __________ Rev #3 5 b. What does Diploid mean? c. What does Haploid mean? Assume an organism has 20 chromosomes: d. How many would be present in the cells after meiosis? e. How many would be present in the cells after mitosis? f. How many would be present in a zygote? Label the diagrams as mitosis, meiosis, or fertilization. Make sure to explain. n 2n a. 2n b. n n + n 2n c. n 2n n 2n n Rev #3 6 ANS KEY Biology EOC Review #3 North Carolina Test of Biology 4. __D___ 21. __C___ 45. __D___ Test 1: 34. _C___ 5. __A___ 25. __B___ 50. __B___ 41. __C___ 14. __B___ 7. __C___ 28. __A___ 53. __A___ 50. __A___ 15. __A__& 23. __A__ name: _____________ Purple Book: Biology Test 2: 11. __B___ EOC Biology Sample Items Goal 2____________________________________________________________ 10. __B____ 11. ___A___ 12. __C___ 13. ___A__ EOC Biology Sample Items Goal 3 _________________________ 1. __C___ 6. __A___ 11. __D___ 16. __C__ 21. _B_ 2. __B___ 7. __D___ 12. __C___ 17. __D__ 22. _D_ 3. __A___ 8. __A___ 13. __A___ 18. __A__ 23. _B_ 4. __D___ 9. __B___ 14. __D___ 19. __C__ 5. __A___ 10. _B___ 15. __A___ 20. __D__ & 23. __B___ 2.03 Investigate and analyze the cell as a living system including: maintenance of homeostasis, movement of materials into and out of cells, and energy use and release in biochemical reactions. Energy, Photosynthesis, and Cellular Respiration ATP: adenosine triphospate – usable form of energy for living organisms ADP: adenosine diphosphate – possesses no energy ATP ADP + P reaction showing the release of energy (energy is stored in the third phosphate) ADP + P ATP reaction showing the capture or gain of energy Photosynthesis o Definition: absorption of sunlight energy to make glucose (food) o Organisms that use photosynthesis: plants, phytoplankton, and bacteria o Summative formula for photosynthesis: Energy + carbon dioxide (CO2) + water (H2O) glucose (C6H12O6) + oxygen (O2) o Location: chloroplast o Pigments: absorb sunlight energy; chlorophyll is an example of a pigment o Plants absorb 6-carbon dioxide molecules; they use the carbons (C) to make glucose Cellular respiration o Definition: breakdown of glucose to make energy (ATP) o Organisms that use cellular respiration: every living thing, including plants o Summative formula for cellular respiration: Glucose (C6H12O6) + oxygen (O2) Energy + carbon dioxide (CO2) + water (H2O) o Location: mitochondria o Anaerobic respiration: reaction that occurs without oxygen present; produces little ATP o Fermentation: Breaking down of “leftover” glucose as waste; alcohol and lactic acid fermentation o Aerobic respiration: reaction that occurs with oxygen; produces the MOST ATP Use the following diagram to show where energy is released and where energy is used. Also use arrows on the lines attached to the circles to indicate the direction of the energy. a. How many phosphates? ATP? ___3______ ADP? ____2____ b. What is ATP energy used for? Give examples. ANYTHING THAT REQUIRES ENERGY c. What cellular process produces ATP? CELL RESPIRATION Rev #3 7 2.05 Investigate and analyze the bioenergetic reactions: aerobic respiration, anaerobic respiration, and photosynthesis. Section 1: What are the reactants and products for each of these? Process Reactant Product Example? Photosynthesis CO2, H20, LIGHT C6H12O6 (SUGAR), O2 PLANTS ENERGY Cellular Respiration C6H12O6 (SUG), O2 CO2, H2O, ATP CONSUMERS, (Aerobic) ANIMALS Cellular Respiration C6H12O6 (SUG), O2 ATP, LACTIC ACID, YEAST, BACTERIA (Anaerobic) CO2, ETHYL ALCOHOL Label the following molecules in these equations (water, glucose, oxygen, carbon dioxide, ethyl alcohol) A) WA TER, CARBON DIOXIDE, GLUCOSE, OXYGEN B) SUGAR, OXYGEN a. A b. B c. A d. CARBON DIOXIDE, WATER Which of the above reactions is photosynthesis? Which of the above reactions is cellular respiration (aerobic)? Which reaction(s) requires or stores energy? Which reaction requires chlorophyll? e. Which reaction requires light? GLUCOSE) A A What is the purpose of the chlorophyll? ABSORB LIGHT ENERGY What is the light used for? ENERGY PRODUCTION (MAKING f. Which organisms carry out process A? PRODUCERS (PLANTS) g. Which organisms carry out process B? HETEROTROPHS (ANIMALS) h. Which process uses chloroplasts in eukaryotes? A i. Which process uses mitochondria in eukaryotes? B j. What factors could speed up (or slow down) process A? AVAILABLE LIGHT, CO2, TEMP, PH k. What factors could speed up (or slow down) process B? AMT OF AVAILABLE GLUCOSE/O2, TEMP, PH Rev #3 8 3.01: Analyze the molecular basis of heredity including: DNA replication, Protein Synthesis (transcription and translation), and gene regulation. DNA DNA – genetic make-up of organisms Monomers of DNA are nucleotides DNA nucleotide is made up of three components: sugar (deoxyribose), phosphate, and nitrogen base (adenine, thymine, guanine, and cytosine) Nitrogen bases base pair or combine -Adenine (A) pairs with Thymine (T) and Guanine (G) pairs with Cytosine (C) A=T and G=C Watson and Crick discovered the shape of DNA – it is a double helix which is shaped like a twisted ladder. The handles or sides of the ladder are made up of phosphate and sugar and the steps are the nitrogen bases (A, T, G, C) DNA replication: process in which DNA is copied. Process: (a) the nitrogen bases (steps) are separated (b) DNA polymerase (enzyme) adds complementary nitrogen bases to the exposed sides. Protein synthesis Protein synthesis is the process in which a gene from DNA is “read” and made into a protein. RNA (ribonucleic acid) is the main character in the process of protein synthesis. RNA is different from DNA is three ways: 1) it is single stranded (DNA double stranded) 2) is has ribose (DNA has dexoyribose) 3) it has uracil instead of thymine Three types of RNA: 1) mRNA (messenger RNA): copies message from DNA 2) tRNA (transfer RNA) brings amino acids to mRNA 3) rRNA (ribosomal RNA) found in ribosome and forms peptide bonds between amino acids Two processes in protein synthesis: o Transcription: takes place in the nucleus; mRNA copies the “message” from DNA If DNA read ATG/GGC/AAA/CTT; the complementary mRNA strand UAC/CCG/UUU/GAA o Translation: takes place in the cytoplasm on a ribosome; tRNA brings the matching amino acids to the codons found on mRNA Codon: three base sequence found on mRNA Anticodon: three base sequence found on tRNA To find the matching amino acids, use the chart of mRNA codons that will be provided. The amino acid sequence for the mRNA strand UAC/CCG/UUU/GAA would be as follows: Tyr-ProPhe-Glu Please remember that when using the chart USE MRNA CODONS!!!! Mutation: change in DNA Two types of mutations: 1) Point mutation: when there is an incorrect base within DNA; only one POINT of DNA is affected; for example, DNA sequence ATG and following replication sequence changes to AAG 2) Frameshift mutation: when a base is deleted or inserted into DNA; causes the whole sequence of DNA to change which leads to an incorrect protein; for example DNA sequence is ATG and following replication the sequence is AAT G…..; the A was inserted into DNA Rev #3 9 Section 2: a. Below is a strand of DNA. DNA in the cells exists as a double helix – what else needs to be added to this strand to make it a double helix? Give the nucleotide sequence. ANOTHER STRAND, TCGGTC b. What are the black pentagons? DEOXYRIBOSE c. What are the nitrogen bases? GACCGA d. If the strand of DNA above undergoes transcription, what will the sequence of the mRNA be? CUGGCU e. After translation, what would the amino acid sequence be for this section of mRNA? (read from right to left) CEU-ALA (ALANINE) f. What is a codon? 3 BASE SEQUENCE ON MRNA THAT CODES FOR A PROTEIN g. Compare RNA and DNA in the following table RNA RIBOSE DNA DEOXYRIBOSE A, U, G, C A, T, G, C ONE TWO NUCLEUS, CYTOPLASM NUCLEUS Sugars Bases Strands Where In Cell? h. What kind of bond hold the amino acids together in the protein that is formed? PEPTIDE BOND i. What are the three types of RNA and what are their functions? MRNA-COPY GENE ON DNA, TRNA-BRING AMINO ACIDS TO MRNA, RRNA-USED IN MAKING PROTEINS (PROTEIN SYNTHESIS) j. What kind of weak bonds hold the two strands of DNA together? HYDROGEN BONDS k. Why is it important that these bonds be weak? SO DNA CAN UNZIP TO REPLICATE/COPY ITSELF l. What happens to DNA when a mutation occurs? INCORRECT BASE IN DNA m. How does this affect the mRNA? WRONG SEQUENCE n. How can this affect translation? WRONG PROTEIN WILL BE MADE Rev #3 10 o. How does this affect the structure and shape of the resulting protein? INCORRECT OR MUTATED PROTEIN (NOT RIGHT FOR THE JOB IT WAS SUPPOSED TO DO) 3.02 Compare and contrast the characteristics of asexual and sexual reproduction. Mitosis and Meiosis Diploid (2n) – normal number of chromosomes within an organism; represents homologous pairs (chromosome from mom and dad); the majority of the cells in your body are 2n Haploid (n) – half the number of chromosomes; the only cells in an organism that are n are the gametes or sex cells (sperm and egg) Mitosis produces an identical copies following division Meiosis produces different cells with half the number of chromosomes Mitosis Meiosis Type of reproduction Asexual: one individual makes Sexual: two individuals another Chromosome # of mother cell (mother cell is Diploid (2n) Diploid (2n) original cell before division) Chromosome # of daughter cells (resulting Diploid (2n) Haploid (n) cells after division) # of cell divisions 1 2 # of cells produced 2 4 Section 3: Type of reproduction (Asexual or sexual) Chromosome number of mother cell (1N=haploid or 2N=diploid) Chromosome number of daughter cells (1N=haploid or 2N=diploid) Number of cell divisions Number of cells produced MITOSIS ASEXUAL SEXUAL 2N 2N 2N N ONE TWO 2 4 When does replication happen? INTERPHASE SOURCES OF VARIATION Crossing over NO Random assortment of NO chromosomes Gene mutations YES Nondisjunction (INCORRECT NO SEPARATION OF CHROMOSOMES) Fertilization NO Put the following stages of mitosis (cell division) in order. MEIOSIS INTERPHASE YES YES YES YES YES 1st: ___C____ called __INTERPHASE______ 2nd: ___B____ called __PROPHASE_____ 3rd: ___E____ called __METAPHASE_____ 4th: ___A____ called __ANAPHASE______ 5TH-D-TELOPHASE Rev #3 11 a. How many chromosomes do humans have in their… Body cells? __46______ Sex cells? ___23_______ b. What does Diploid mean? 2N; HOMOLOGOUS PAIRS PRESENT c. What does Haploid mean? N-HALF THE NUMBER OF CHROMOSOMES (LIKELY A SEX CELL) Assume an organism has 20 chromosomes: d. How many would be present in the cells after meiosis? 10 e. How many would be present in the cells after mitosis? 20 f. How many would be present in a zygote? 20 Label the diagrams as mitosis, meiosis, or fertilization. Make sure to explain. MITOSIS FERTILIZATION MEIOSIS 2n a. 2n b. n n + n 2n c. n n 2n n 2n n Rev #3 12