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Chapter 9 Lecture Chemical Energy/ Cell Respiration Food provides chemical building blocks to make muscle and new cells. It also provides energy for growth and movement. There is a lot of energy in food. One gram of sugar had 4 Calories which is 4000 calories. The capital “C” means it is a kilocalorie. 1C= 1000 c. Our ___________________measured in Kilocalories. Our body slowly releases this energy in the form of ______________________ (Latin: Sugar-cutting). This slow release is important. You wouldn’t want your car to burn up all the gasoline at once. The same is true with your body. Cellular respiration is the process that releases energy by breaking down food molecules and it must have __________________________. Key Notes ATP, used for cell energy NADH: Electrons holders to be converted later top ATP FADH: Electrons holders to be converted later top ATP Glycolysis takes place in the _________________________. The other stage of the cell respiration takes place inside the mitochondria and the cell membrane of the mitochondrion. 6O2+ C6H12O6 6CO2 + 6H2O + energy Notice the formula is opposite from ___________________________ GLYCOLYSIS One glucose molecule (C2H12O6) is broken in half, which are 2 molecules of __________ ________ Glucose Needs Energy ______ATP Units Pyruvic Acid (next stop Krebs Cycle) Releases _____ATP Units To break the glucose in half requires 2 units of ATP energy. When the 3 carbon molecules are rearranged however, they get 4 ATP, for a net gain of 2 ATP. NADH is another energy ________________________ molecule. It holds the electrons that are removed from the 2 pyruvic acid molecules and then later ____________________ them to create more ATP in the mitochondrion. Thousands of ATP each millisecond can be produced in Glycolysis. It doesn’t even need oxygen from the cell. This part of cellular respiration is ___________________________. If oxygen is present the 2 pyruvic acids go to the_______________________. Sometimes oxygen is not present in the cell or not enough oxygen is present. When this lack of oxygen occurs, the 2 pyruvic acids go through a process called _________________________. Step 1: The NADH electron holder gives back the 2 electrons to the Pyruvic acids.(pg 224) Step 2: the e- enhanced pyruvic acid molecules can go to lactic acid fermentation or alcoholic fermentation. Step 3: Alcoholic or Lactic acids fermentation (only yeast and bacteria create _________________) Alcoholic Fermentation Bread, Wine, Alcohol Pyruvic acid + NADH alcohol + CO2 + NAD+ Bread / Pizza dough rises because of AL-Ferm. CO2 is given off in beer and the bread rises due to CO2 bubbles. Alcohol evaporates when the bread is cooked. Lactic Acid Fermentation: L-A Ferm: L.A.F The pyruvic acid is converted into lactic acid which is still 3-carbon molecule, but it has a different structural shape. L.A build up is what makes ____________________ store and burn. It takes a few days for the LA to be broken down in the muscle cells. Krebs’s Citric Acid Cycle Nobel prize 1953 ( If there is Oxygen!) Aerobic- requires oxygen There are parts- steps involved to get ALL the energy from the pyruvic acid. Takes place in the mitochondrion Phase A pg227 1. Py-A, enters the mitochondrion and ________________ one carbon to create CO2. 2. The 2 carbon molecule attach to a Co-enzyme ( part vitamin/ part enzyme) 3. Acetyl-Co enzyme connects to the 4 carbon citric acid molecules- the first molecule of the Krebs cycle. ( the Co-enzyme A detaches to be reused) Phase B 1. Citric acid and the Acetyl formed a 6 carbon molecule but one Carbon is released as Co2. 2. This allows energy ( electrons) to be stored by NADH 3. the citric acid removes 2 more carbon which create more NADH and one ATP Phase C. Net profit of one Pyruvic acid molecule 1 _________ 3 _________ 1 _________ Summary of one glucose molecule before electron transport Glycolysis= 2 ATP and 2 NADH ( for both pyruvic acids) Krebs Cycle= 2 ATP, 8 NADH, 2 FADH2 Before going to the ETC ( electron transport chain) 2 units of ATP- usable energy 8 NADH 2 FADH stored electron energy to be converted into ATP, by the ETC. The ETC converts high energy electrons into ATP The electrons are removed from the NADH and FADH by integral channel proteins in the cell membrane of the mitochondrion. As each e- is moved from protein to protein it creates water molecules by attaching to H+ ions in the cell. The oxygen that you breathe is where the O2 comes from to create the water. The movement of positive H+ ions and the movement of negative e- given off from NADH, cause the plasma membrane to flip flop from positive to negative inside the mitochondrion. When the inside is negative, an H+ ion enters through the specialized protein channel attached to an enzyme make ADP turn into ATP. On average, each electron (e-) makes 3 ATP. Fill out pg 229 Krebs’ Cycle and ETC Total ATP Produced Energy and Power Shopping(or other Moving Activity) Given: 1. Muscles have a small amount of stored ATP for energy 2. Muscles can get energy by LA- Ferm. 3. Muscles with 2 can get 36 ATP unit from glucose stored in cells Speed shopping- Anaerobic Huge shoe sale! Doors open at 8:00 am! Only one shoe size for each other type of shoe! A certain biology teacher who has a severe shoe habit has to park 300 meters away from the door! There are others that are also in the parking lot. There are others that are also already standing at the door. Ms. Bio must sprint to the door and push her way to the front. Ms. Bio would be using her quick stored energy in her muscles. Sadly, this energy last for a few seconds or in her case 50 meters. Her muscles are now producing lactic acid. However this lactic acid energy will only last for about one minute. After a minute the muscles cannot keep the high speed. Will Ms. Bio make it to the door? Can she sprint faster than the other ladies in the parking lot? No! She wore a pair of 4 inch strappy sandals. The lady who beat her was wearing “sensible” shoes. “Sensible” means ugly shoes. Ms. Bio is determined to buy shoes. She no longer has to sprint. She jogs over to her size. Her muscles are in aerobic cellular respiration. She can shop for hours now and not just for shoes! Real Lesson: Lactic Acid fermentation can only supply a small amount of energy before the muscle fatigues. Or If your muscles cannot get oxygen fast enough for the work load you are requiring, it will resort to anaerobic fermentation. Your muscles have a type of sugar stored called glycogen. This sugar will supply energy for about 20 minutes, after 20 minutes such as all day shopping; other molecules such as fat are used for energy. Sadly one must exercise 20 minutes before any fat is even burned! * Fatty acids and amino acids also enter the Krebs Cycle. Fats produce over 2.5 x the ATP energy of one glucose.