Volume 1, Issue 1, November 2012
... Sciences and Biomedical Research. The journal welcomes research papers, review articles, case studies and short communications allied to the scope of the journal. In order to be considered for publication, a manuscript must meet the general criteria of authenticity, timeliness, purpose and importanc ...
... Sciences and Biomedical Research. The journal welcomes research papers, review articles, case studies and short communications allied to the scope of the journal. In order to be considered for publication, a manuscript must meet the general criteria of authenticity, timeliness, purpose and importanc ...
RNA interference - genemol de Jean
... The machinery is however also used by the cell itself to regulate gene activity: certain parts of the genome are transcribed into microRNA, short RNA molecules that fold back on themselves in a hairpin shape to create a double strand. When the RNA interference machinery detects these double strands, ...
... The machinery is however also used by the cell itself to regulate gene activity: certain parts of the genome are transcribed into microRNA, short RNA molecules that fold back on themselves in a hairpin shape to create a double strand. When the RNA interference machinery detects these double strands, ...
Biotechnology - Education Vijay
... best suited crops, having the highest yields, to produce enough food to support a growing population. As crops and fields became increasingly large and difficult to maintain, it was discovered that specific organisms and their by-products could effectively fertilize, restore nitrogen, and control pe ...
... best suited crops, having the highest yields, to produce enough food to support a growing population. As crops and fields became increasingly large and difficult to maintain, it was discovered that specific organisms and their by-products could effectively fertilize, restore nitrogen, and control pe ...
Southern Analysis: - California State University San Marcos
... 0.1 M Tris (pH 7.5), 0.15 M NaCl 1 min 0.1 M Malate, 0.15 M NaCl,0.5% ...
... 0.1 M Tris (pH 7.5), 0.15 M NaCl 1 min 0.1 M Malate, 0.15 M NaCl,0.5% ...
DNA Dots - miniPCR
... pig’s organs much more similar to those of humans. This was the first step in trying to create pig organs that could potentially be used for transplantation in humans! Researchers are also utilizing CRISPR/Cas9 to create animal models of human diseases, allowing them to engineer model animals in a w ...
... pig’s organs much more similar to those of humans. This was the first step in trying to create pig organs that could potentially be used for transplantation in humans! Researchers are also utilizing CRISPR/Cas9 to create animal models of human diseases, allowing them to engineer model animals in a w ...
+SDS - Creighton Chemistry Webserver
... Serum albumin - protein used to transport nonsoluble molecules through the bloodstream Carries: bilirubin, fatty acids, hormones, dyes ...
... Serum albumin - protein used to transport nonsoluble molecules through the bloodstream Carries: bilirubin, fatty acids, hormones, dyes ...
Bio-Chemistry Course Descriptions
... Fayetteville State University Department: Natural Sciences Program: Biochemistry Courses Course Descriptions ...
... Fayetteville State University Department: Natural Sciences Program: Biochemistry Courses Course Descriptions ...
MOLECULAR BIOLOGY AND BIOTECHNOLOGY
... MOLECULAR BIOLOGY AND BIOTECHNOLOGY Course Contnts MBB 501 PRINCIPLES OF BIOTECHNOLOGY 3+0 SEM - I ObjectiveT o familiarize the students with the fundamental principles of Biotechnology, various developments in Biotechnology and its potential applications. Theory UNIT-I: History, scope and importanc ...
... MOLECULAR BIOLOGY AND BIOTECHNOLOGY Course Contnts MBB 501 PRINCIPLES OF BIOTECHNOLOGY 3+0 SEM - I ObjectiveT o familiarize the students with the fundamental principles of Biotechnology, various developments in Biotechnology and its potential applications. Theory UNIT-I: History, scope and importanc ...
Synthetic Biology: Gene Transformation and Protein Purification
... – Bioremediation: bacteria can be transformed to have genes that enable them to digest oil spills – Production of medicine: bacteria can be transformed to produce proteins like insulin to help diabetics. ...
... – Bioremediation: bacteria can be transformed to have genes that enable them to digest oil spills – Production of medicine: bacteria can be transformed to produce proteins like insulin to help diabetics. ...
A Conversation about Central Dogma of Molecular
... of sequence-based information? After all the 3dimensional structure of a prion is dependent on its sequence, and a prion ‘converts’ the structure of only those ‘normal’ proteins that have the same sequence as the prion protein. Teacher: It is indeed true that the sequence of a prion protein is a per ...
... of sequence-based information? After all the 3dimensional structure of a prion is dependent on its sequence, and a prion ‘converts’ the structure of only those ‘normal’ proteins that have the same sequence as the prion protein. Teacher: It is indeed true that the sequence of a prion protein is a per ...
Laboratory Analytical Methods
... difference of sibling pairs (n=322) on the proportion of alleles shared identical-by-descent. Because sibpairs in the same sibship may be nonindependent statistically, significance levels are calculated using effective degrees of freedom (edf), which reflects the number of independent sibling pairs ...
... difference of sibling pairs (n=322) on the proportion of alleles shared identical-by-descent. Because sibpairs in the same sibship may be nonindependent statistically, significance levels are calculated using effective degrees of freedom (edf), which reflects the number of independent sibling pairs ...
Microfluidic Tools for Systems Biology
... powerful as PCR and gene microarrays used in genomic research. With limited amounts of sample available covering a broad spectrum of protein concentrations, analytical precision and efficient sample use become critical experimental factors [3], [7]. Estimates for the number of different proteins pro ...
... powerful as PCR and gene microarrays used in genomic research. With limited amounts of sample available covering a broad spectrum of protein concentrations, analytical precision and efficient sample use become critical experimental factors [3], [7]. Estimates for the number of different proteins pro ...
emboj7601486-sup
... Equilibrium-flotated vacuoles were diluted in 0% Ficoll buffer (10 mM PIPES-KOH [pH 6.8], 200 mM sorbitol) and collected by centrifugation. The pelleted vacuoles were lysed in NP-40 lysis buffer (50 mM Tris-HCl [pH 8], 0.5% NP-40, 150 mM NaCl, 5 mM MgCl2) containing the COMPLETE protease inhibitor c ...
... Equilibrium-flotated vacuoles were diluted in 0% Ficoll buffer (10 mM PIPES-KOH [pH 6.8], 200 mM sorbitol) and collected by centrifugation. The pelleted vacuoles were lysed in NP-40 lysis buffer (50 mM Tris-HCl [pH 8], 0.5% NP-40, 150 mM NaCl, 5 mM MgCl2) containing the COMPLETE protease inhibitor c ...
High-Resolution Array-Based Comparative Genomic Hybridization
... borderline melanocytic lesions where prolonged follow-up over many years may be required to ensure benign biologic behavior (ie, absence of metastasis). Gene expression profiling experiments use mRNA harvested from fresh tumor tissue, from which cDNAs are produced and used for hybridization. They ha ...
... borderline melanocytic lesions where prolonged follow-up over many years may be required to ensure benign biologic behavior (ie, absence of metastasis). Gene expression profiling experiments use mRNA harvested from fresh tumor tissue, from which cDNAs are produced and used for hybridization. They ha ...
FORESTRY SCIENCES KLUWER ACADEMIC PUBLISHERS
... Most of the floral homeotic genes belong to the evolutionarily conserved MADS box gene family. The MADS box is a highly conserved protein domain involved in DNA binding and protein dimerization, and is found in regulatory proteins of yeast, plants, and animals. A number of other MADS box genes, term ...
... Most of the floral homeotic genes belong to the evolutionarily conserved MADS box gene family. The MADS box is a highly conserved protein domain involved in DNA binding and protein dimerization, and is found in regulatory proteins of yeast, plants, and animals. A number of other MADS box genes, term ...
Molecular Diagnostics as Basis of Personalized Medicine
... clinically relevant and would fit in with the coming trend in individualized treatment. These technologies have an advantage in diagnosis of some conditions. For example, different proteins such as antibodies, antigens, and enzymes can be immobilized within protein microchips. Miniaturized and highl ...
... clinically relevant and would fit in with the coming trend in individualized treatment. These technologies have an advantage in diagnosis of some conditions. For example, different proteins such as antibodies, antigens, and enzymes can be immobilized within protein microchips. Miniaturized and highl ...
Assay Standards Working Group Recommendations, November 2012
... Recent technological advancements have enabled the reproducible assessment of epigenomic marks across the entire genome of human cells, and large-scale international efforts are now underway to generate high-resolution reference epigenome maps to accelerate the scientific exploitation of human epige ...
... Recent technological advancements have enabled the reproducible assessment of epigenomic marks across the entire genome of human cells, and large-scale international efforts are now underway to generate high-resolution reference epigenome maps to accelerate the scientific exploitation of human epige ...
Expression of gene encoding immunodominant merozoite surface
... 1,2 or 5 µg per mL of carbonate-bicarbonate buffer (pH 9.6) and titrated with two positive and two negative bovine sera diluted 1:50, 1:100 or 1:200 with PBS (pH 7.2) in standard ELISA format10. The combination of antigen and dilutions of serum, which yielded maximum differences in OD values of nega ...
... 1,2 or 5 µg per mL of carbonate-bicarbonate buffer (pH 9.6) and titrated with two positive and two negative bovine sera diluted 1:50, 1:100 or 1:200 with PBS (pH 7.2) in standard ELISA format10. The combination of antigen and dilutions of serum, which yielded maximum differences in OD values of nega ...
Microsoft Word
... glucose isomerase, xylanase, protease etc. by Streptomyces fermentation and the potential for improving the economics of these processes have stimulated interest in the application of novel genetic techniques to Streptomyces species. Recombinant DNA technology offers a means to isolate and manipulat ...
... glucose isomerase, xylanase, protease etc. by Streptomyces fermentation and the potential for improving the economics of these processes have stimulated interest in the application of novel genetic techniques to Streptomyces species. Recombinant DNA technology offers a means to isolate and manipulat ...
Plasmid pIP501 Encoded Transciptional Repressor CopR Binds to
... to 20-fold higher than KD for CopR-dimer dissociation and suggests that CopR is present mainly as a dimer in B. subtilis cells. Therefore, we suggest that CopR in vivo is likely to bind to its operator as a preformed dimer. As shown previously, CopR reduces the plasmid copy number about 10 to 20-fol ...
... to 20-fold higher than KD for CopR-dimer dissociation and suggests that CopR is present mainly as a dimer in B. subtilis cells. Therefore, we suggest that CopR in vivo is likely to bind to its operator as a preformed dimer. As shown previously, CopR reduces the plasmid copy number about 10 to 20-fol ...
Analysis of 16S rRNA Gene of Lactic Acid
... organisms is also warranted. Since the 16S rRNA gene has hypervariable regions which are species specific, the most dependable and widely used techniques for bacterial identification are based on the 16S rRNA gene [9][11].Since these hypervariable regions are also often flanked by strongly conserved ...
... organisms is also warranted. Since the 16S rRNA gene has hypervariable regions which are species specific, the most dependable and widely used techniques for bacterial identification are based on the 16S rRNA gene [9][11].Since these hypervariable regions are also often flanked by strongly conserved ...
6th Annual GS-LSAMP Phase II * NNJ
... Polymerase chain reaction that replicates DNA strands, forward and reverse primers put into two separate tubes containing DNA, dNTPs, and ddNTPs in order to achieve Sanger sequencing, reproduction of a particular segment of the gene was possible… and with the DNA sequence analyzer we were able to ob ...
... Polymerase chain reaction that replicates DNA strands, forward and reverse primers put into two separate tubes containing DNA, dNTPs, and ddNTPs in order to achieve Sanger sequencing, reproduction of a particular segment of the gene was possible… and with the DNA sequence analyzer we were able to ob ...
Table SI. Primers used for creation of the PHAC1co and
... Transformants were selected on SD medium. Correct integration of the UGT1co expression cassette at the UGTB1 locus was controlled by two yeast colony PCR’s. Primers KIUGT1 CtrlFor2 and KIUGT1CtrlRev were used to check 3’ cross-over while primers Ura3endFor and GTII+239Rev were used to check cross-ov ...
... Transformants were selected on SD medium. Correct integration of the UGT1co expression cassette at the UGTB1 locus was controlled by two yeast colony PCR’s. Primers KIUGT1 CtrlFor2 and KIUGT1CtrlRev were used to check 3’ cross-over while primers Ura3endFor and GTII+239Rev were used to check cross-ov ...
The dog genome - Macmillan Learning
... Genome Project began in the late 1990s. Since then the sequences of several dog genomes have been published. Two dogs—a boxer and a poodle—were the first to have their entire genomes sequenced. The dog genome contains 2.8 billion base pairs of DNA in 39 pairs of chromosomes. There are 19,000 protein ...
... Genome Project began in the late 1990s. Since then the sequences of several dog genomes have been published. Two dogs—a boxer and a poodle—were the first to have their entire genomes sequenced. The dog genome contains 2.8 billion base pairs of DNA in 39 pairs of chromosomes. There are 19,000 protein ...
Bioinformatics
Bioinformatics /ˌbaɪ.oʊˌɪnfərˈmætɪks/ is an interdisciplinary field that develops methods and software tools for understanding biological data. As an interdisciplinary field of science, bioinformatics combines computer science, statistics, mathematics, and engineering to analyze and interpret biological data.Bioinformatics is both an umbrella term for the body of biological studies that use computer programming as part of their methodology, as well as a reference to specific analysis ""pipelines"" that are repeatedly used, particularly in the fields of genetics and genomics. Common uses of bioinformatics include the identification of candidate genes and nucleotides (SNPs). Often, such identification is made with the aim of better understanding the genetic basis of disease, unique adaptations, desirable properties (esp. in agricultural species), or differences between populations. In a less formal way, bioinformatics also tries to understand the organisational principles within nucleic acid and protein sequences.