The BCM Microarray Core Facility
... single-reads, short- and long-insert paired-end reads for whole-genome sequencing and resequencing, SNP discovery, identification of copy number variations and chromosomal rearrangements. ...
... single-reads, short- and long-insert paired-end reads for whole-genome sequencing and resequencing, SNP discovery, identification of copy number variations and chromosomal rearrangements. ...
Myriad - Tech Transfer Central
... Methods that “compare” and “correlate” mutations in the BRCA genes with an increased risk of breast or ovarian cancer. Claim 2 of US Patent 6,033,857 is representative of this class of claims: A method for diagnosing a predisposition for breast cancer in a human subject which comprises comparing the ...
... Methods that “compare” and “correlate” mutations in the BRCA genes with an increased risk of breast or ovarian cancer. Claim 2 of US Patent 6,033,857 is representative of this class of claims: A method for diagnosing a predisposition for breast cancer in a human subject which comprises comparing the ...
No Slide Title
... • Enables viruses to integrate their genetic material into a host’s genome • Enables host organism to assort alleles (differing copies of same gene) into novel groups - favorable & unfavorable alleles can be shuffled randomly • Enables repair of a damaged gene in an otherwise favorable chromosome • ...
... • Enables viruses to integrate their genetic material into a host’s genome • Enables host organism to assort alleles (differing copies of same gene) into novel groups - favorable & unfavorable alleles can be shuffled randomly • Enables repair of a damaged gene in an otherwise favorable chromosome • ...
macromolecules test 1
... the victim and suspect B. the victim, suspect A, and suspect B. the victim and an unknown individual. ...
... the victim and suspect B. the victim, suspect A, and suspect B. the victim and an unknown individual. ...
Document
... -determine lengths of DNA 2-IDENTIFY the FUNCTION of GEL ELECTROPHORESIS? -SORT & MEASURE DNA strands by length 3-How does GE work? -gel acts as a filter -separates molecules (electrical charge) -sorts DNA strands (banded pattern) SEQUENCE steps of DNA moving thru gel ...
... -determine lengths of DNA 2-IDENTIFY the FUNCTION of GEL ELECTROPHORESIS? -SORT & MEASURE DNA strands by length 3-How does GE work? -gel acts as a filter -separates molecules (electrical charge) -sorts DNA strands (banded pattern) SEQUENCE steps of DNA moving thru gel ...
Nucleic acid crystallography: current progress
... over the past two years makes clear the incredibly versatile nature of nucleic acid tertiary structure. Long gone are the days when it was possible to group nucleic acid crystal structures simply into A-, B- and Z-form DNA double helices [3]. But even in the case of double helical fragments, it is n ...
... over the past two years makes clear the incredibly versatile nature of nucleic acid tertiary structure. Long gone are the days when it was possible to group nucleic acid crystal structures simply into A-, B- and Z-form DNA double helices [3]. But even in the case of double helical fragments, it is n ...
Electrophoresis and Hardy Wienberg notes
... The portion of human DNA that forms genes which code for proteins or RNA molecules is very small – over 98% of human DNA is considered to be non-coding DNA. For a long time, the function of this DNA was unknown, leading to the term junk DNA becoming popular. Scientists now believe that much of this ...
... The portion of human DNA that forms genes which code for proteins or RNA molecules is very small – over 98% of human DNA is considered to be non-coding DNA. For a long time, the function of this DNA was unknown, leading to the term junk DNA becoming popular. Scientists now believe that much of this ...
Lecture NoteIV
... plasmid DNA during the plasmid replication process by enzymes called topoisomerases. The supercoiled conformation can be maintained when both polynucleotide strands are intact, hence called covalently closed-circular (ccc) DNA. If one of the polynucleotide strands is broken, the double helix reverts ...
... plasmid DNA during the plasmid replication process by enzymes called topoisomerases. The supercoiled conformation can be maintained when both polynucleotide strands are intact, hence called covalently closed-circular (ccc) DNA. If one of the polynucleotide strands is broken, the double helix reverts ...
PSI- Genes
... Cells have to reproduce many times. In complex organisms, trillions of copies are made from one original cell. But when cells reproduce, their DNA has to reproduce as well. The structure of DNA reveals how trillions of copies of the DNA in one of your cells can be made, and be almost exactly the sam ...
... Cells have to reproduce many times. In complex organisms, trillions of copies are made from one original cell. But when cells reproduce, their DNA has to reproduce as well. The structure of DNA reveals how trillions of copies of the DNA in one of your cells can be made, and be almost exactly the sam ...
Supplementary Methods
... genes in the A and B samples. It is interesting to note that in every case the Pearson correlation between the microarrays with the transcriptome sequencing is even better (0.76), Affymetrix (0.81), Agilent (0.83), and Illumina (0.79) than with QRTPCR, suggesting that the transcriptome sequencing of ...
... genes in the A and B samples. It is interesting to note that in every case the Pearson correlation between the microarrays with the transcriptome sequencing is even better (0.76), Affymetrix (0.81), Agilent (0.83), and Illumina (0.79) than with QRTPCR, suggesting that the transcriptome sequencing of ...
2014 Personalized Medicine Module Presentation
... DNA variations in special proteins in the liver called enzymes can influence a person’s ability to metabolize certain drugs ...
... DNA variations in special proteins in the liver called enzymes can influence a person’s ability to metabolize certain drugs ...
Introduction to Nucleic Acids
... Structural Properties of Nucleic Acid Building Blocks Function of DNA and RNA DNA and RNA are chainlike macromolecules that function in the storage and transfer of genetic information. They are major components of all cells ~15% of the cells dry weight. Just as the amino acids are building blocks of ...
... Structural Properties of Nucleic Acid Building Blocks Function of DNA and RNA DNA and RNA are chainlike macromolecules that function in the storage and transfer of genetic information. They are major components of all cells ~15% of the cells dry weight. Just as the amino acids are building blocks of ...
Protocol
... the same (two) palindromic oligos can anneal to each other to form a double-strand oligo. This eliminates the need to mix and anneal two different DNA oligos and reduces operational mistakes during the cloning process. The overall chance of mutations introduced during DNA oligo synthesis is also red ...
... the same (two) palindromic oligos can anneal to each other to form a double-strand oligo. This eliminates the need to mix and anneal two different DNA oligos and reduces operational mistakes during the cloning process. The overall chance of mutations introduced during DNA oligo synthesis is also red ...
Next lectures: Differential Gene expression
... initiation complex assembled at the promoter is thought to regulate transcription • Enhancers are modular. Particular combinations of factors (rather than any one factor) determines enhancer function ...
... initiation complex assembled at the promoter is thought to regulate transcription • Enhancers are modular. Particular combinations of factors (rather than any one factor) determines enhancer function ...
ppt - Chair of Computational Biology
... These clusters, or CpG islands, are targets for proteins that bind to unmethylated CpGs and initiate gene transcription. In contrast, methylated CpGs are generally associated with silent DNA, can block methylation-sensitive proteins and can be easily mutated. The loss of normal DNA methylation patte ...
... These clusters, or CpG islands, are targets for proteins that bind to unmethylated CpGs and initiate gene transcription. In contrast, methylated CpGs are generally associated with silent DNA, can block methylation-sensitive proteins and can be easily mutated. The loss of normal DNA methylation patte ...
Understanding Mutation (PowerPoint) WVU 2013
... A small group of animals moves from the mainland to an island, founding a new population. There is no subsequent movement of animals on or off the island. This initial population included coat color variation. Some years afterward, however, a new pattern variation arose that was previously not obser ...
... A small group of animals moves from the mainland to an island, founding a new population. There is no subsequent movement of animals on or off the island. This initial population included coat color variation. Some years afterward, however, a new pattern variation arose that was previously not obser ...
Genetic Technology - McGraw Hill Higher Education
... manufacture short pieces of DNA of any sequence it is programmed to produce. The DNA synthesizer cannot easily make entire genes, but it can make small fragments that can act as primers to DNA replication. If one primer is made for each end of the region of interest, they act to bracket the region t ...
... manufacture short pieces of DNA of any sequence it is programmed to produce. The DNA synthesizer cannot easily make entire genes, but it can make small fragments that can act as primers to DNA replication. If one primer is made for each end of the region of interest, they act to bracket the region t ...
Warm up
... Unit intro and cell types notes Cell types worksheets. Due 10-9 Reading: page 182-189 ...
... Unit intro and cell types notes Cell types worksheets. Due 10-9 Reading: page 182-189 ...
LNA-PNA Comparison4
... oligonucleotide for its complementary RNA or DNA target by increasing the melting temperature (Tm) of the duplex. Additionally, the Tm difference between a perfectly matched target and a mismatched target is substantially higher than that observed when a DNA-based oligonucleotide is used. The proper ...
... oligonucleotide for its complementary RNA or DNA target by increasing the melting temperature (Tm) of the duplex. Additionally, the Tm difference between a perfectly matched target and a mismatched target is substantially higher than that observed when a DNA-based oligonucleotide is used. The proper ...
View Full Text-PDF
... flies, were correlated exactly in how the loss or modification of specific bands of their giant chromosomes (Figure. 1B) were processed. Due to DNA replication without intervention of a cell division, each chromosome in these cells contained more than 1,000 aligned identical DNA molecules. The genes ...
... flies, were correlated exactly in how the loss or modification of specific bands of their giant chromosomes (Figure. 1B) were processed. Due to DNA replication without intervention of a cell division, each chromosome in these cells contained more than 1,000 aligned identical DNA molecules. The genes ...
DNA supercoil
DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.