S4 Text.
... Now that we have isolated good quality genomic DNA from our GAL4 enhancer trap lines, we can perform inverse PCR to identify the locations of the pGawB inserts. We will begin by digesting a portion of our genomic DNA with a restriction enzyme that cuts frequently, HpaII. Recombinant DNA technology w ...
... Now that we have isolated good quality genomic DNA from our GAL4 enhancer trap lines, we can perform inverse PCR to identify the locations of the pGawB inserts. We will begin by digesting a portion of our genomic DNA with a restriction enzyme that cuts frequently, HpaII. Recombinant DNA technology w ...
Breast Cancer
... The alternate forms of a given STR correspond with different alleles. Most STRs occur in gene introns (non-coding regions of DNA), so the variation in the number of repeats does not usually affect gene function, but we can use STRs to differentiate between different alleles. Because pieces of DNA th ...
... The alternate forms of a given STR correspond with different alleles. Most STRs occur in gene introns (non-coding regions of DNA), so the variation in the number of repeats does not usually affect gene function, but we can use STRs to differentiate between different alleles. Because pieces of DNA th ...
Co-dominant SCAR marker, P6-25 - Department of Plant Pathology
... 1 min. These cycles were followed by 72 C for 10 min, and then the reaction was held at 4 C. PCR reactions were performed in the MJ DNA Engine PT200 Thermocycler™ (MJ Research Inc., Waltham, MA). PCR-amplified fragments were separated by gel electrophoresis with 1.5% agarose in 0.5 X TBE buffer, sta ...
... 1 min. These cycles were followed by 72 C for 10 min, and then the reaction was held at 4 C. PCR reactions were performed in the MJ DNA Engine PT200 Thermocycler™ (MJ Research Inc., Waltham, MA). PCR-amplified fragments were separated by gel electrophoresis with 1.5% agarose in 0.5 X TBE buffer, sta ...
preimplantation genetic diagnosis
... blastomere is aspirated and removed from the embryo for analysis. Diagnosing one or two cells isolated from 8-16 cell embryos may occasionally fail to detect mosaicism. ...
... blastomere is aspirated and removed from the embryo for analysis. Diagnosing one or two cells isolated from 8-16 cell embryos may occasionally fail to detect mosaicism. ...
5. Harmful mutations
... The conformation that DNA adopts depends on the hydration level, DNA sequence, the amount and direction of supercoiling, chemical modifications of the bases, the type and concentration of metal ions, as well as the presence of polyamines in solution. 4. DNA replication DNA replication, the basis for ...
... The conformation that DNA adopts depends on the hydration level, DNA sequence, the amount and direction of supercoiling, chemical modifications of the bases, the type and concentration of metal ions, as well as the presence of polyamines in solution. 4. DNA replication DNA replication, the basis for ...
Chapter 19 (Eukaryotic Genome)
... – Is ordered into higher structural levels than – The DNA-protein complex in prokaryotes ...
... – Is ordered into higher structural levels than – The DNA-protein complex in prokaryotes ...
Sequence Enhancer Information - Garvan Institute of Medical
... In this report we have demonstrated the powerful effects of betaine, dimethyl sulfoxide, and 7-deaza-dGTP in combination on the amplification of three sequences with a high GC content. These molecules have been shown in the past to enhance amplification separately or in combinations of two, such as ...
... In this report we have demonstrated the powerful effects of betaine, dimethyl sulfoxide, and 7-deaza-dGTP in combination on the amplification of three sequences with a high GC content. These molecules have been shown in the past to enhance amplification separately or in combinations of two, such as ...
Student Materials - Scope, Sequence, and Coordination
... 4. If you were to make a DNA molecule with six amino acids, do you think that any other team in the room would make the identical molecule? Justify your answer. 5. Many of the amino acids have more than one triplet to code for. Some will have as many as six different codes. Why do you think that it ...
... 4. If you were to make a DNA molecule with six amino acids, do you think that any other team in the room would make the identical molecule? Justify your answer. 5. Many of the amino acids have more than one triplet to code for. Some will have as many as six different codes. Why do you think that it ...
lecture 3
... Non-autonomous (successful freeloaders! ‘borrow’ RT from other sources such as LINEs) ~100-300bp long Internal polymerase III promoter No proteins Share 3’ ends with LINEs 3 related SINE families in humans – active Alu, inactive MIR and Ther2/MIR3. ...
... Non-autonomous (successful freeloaders! ‘borrow’ RT from other sources such as LINEs) ~100-300bp long Internal polymerase III promoter No proteins Share 3’ ends with LINEs 3 related SINE families in humans – active Alu, inactive MIR and Ther2/MIR3. ...
nucleosome antigen - Arotec Diagnostics
... have been found (with a lower prevalence than in SLE) in a number of other autoimmune diseases6,9,10,19 such as systemic sclerosis, Sjogren's syndrome and mixed connective tissue disease and are also found in 40-50% of patients with autoimmune hepatitis type I11,12. Anti-ribosomal P antibodies have ...
... have been found (with a lower prevalence than in SLE) in a number of other autoimmune diseases6,9,10,19 such as systemic sclerosis, Sjogren's syndrome and mixed connective tissue disease and are also found in 40-50% of patients with autoimmune hepatitis type I11,12. Anti-ribosomal P antibodies have ...
GFP plasmid - Kiwi.mendelu.cz
... exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range ...
... exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range ...
The Structure and Function of the DNA from Bacteriophage Lambda
... metric units is 13.2 microns. Under these conditions, normal lambda DNA exhibits a distribution centering at 14.5 to 15 microns (4). The above population of lambda dg wholes represents 58 closed molecules. Six additional closed molecules were observed, but these had lengths between 24.5 and 28 micro ...
... metric units is 13.2 microns. Under these conditions, normal lambda DNA exhibits a distribution centering at 14.5 to 15 microns (4). The above population of lambda dg wholes represents 58 closed molecules. Six additional closed molecules were observed, but these had lengths between 24.5 and 28 micro ...
Rolling circle transcription on smallest size double stranded DNA
... Transcription kinetics of the RNAP T7 on small circular templates .................................................. 30 Summary and prospects .................................................................................................................... 35 Materials and methods ................ ...
... Transcription kinetics of the RNAP T7 on small circular templates .................................................. 30 Summary and prospects .................................................................................................................... 35 Materials and methods ................ ...
Enzyme Mechanisms - Illinois Institute of Technology
... Compare L for real DNA to what it would be if it were relaxed (W=0): That’s L = L - L0 Sometimes we want = superhelix density = specific linking difference = L / L0 Natural circular DNA always has < 0 ...
... Compare L for real DNA to what it would be if it were relaxed (W=0): That’s L = L - L0 Sometimes we want = superhelix density = specific linking difference = L / L0 Natural circular DNA always has < 0 ...
D melanogaster - GEP Community Server
... 1. Satellite DNA, a sequence of tandem repeats, is very difficult to sequence, as there are few markers to help order subclones; hence centromeric regions of the chromosomes are usually left unsequenced. 2. Other repetitious DNA, derived from transposable elements, also causes difficulties; because ...
... 1. Satellite DNA, a sequence of tandem repeats, is very difficult to sequence, as there are few markers to help order subclones; hence centromeric regions of the chromosomes are usually left unsequenced. 2. Other repetitious DNA, derived from transposable elements, also causes difficulties; because ...
DNA mutation bracelets
... 1) What changes in the RNA sequence were caused by the changes in the DNA? When the DNA sequence was changed, the RNA reading frame was shifted. 2) What changes in amino acids were caused by the changes in the DNA? Different amino acid were coded after the deletion shifted the sequence. 3) What happ ...
... 1) What changes in the RNA sequence were caused by the changes in the DNA? When the DNA sequence was changed, the RNA reading frame was shifted. 2) What changes in amino acids were caused by the changes in the DNA? Different amino acid were coded after the deletion shifted the sequence. 3) What happ ...
Novel Roles for Selected Genes in Meiotic DNA Processing
... A large number of high-throughput analyses have been performed to characterize the 6,200 genes of S. cerevisiae. These have included genomic screens for protein–protein [1– 3] and protein complex interactions [4–7], high-throughput genetic interaction analyses [8–13], genome-wide measurements of gen ...
... A large number of high-throughput analyses have been performed to characterize the 6,200 genes of S. cerevisiae. These have included genomic screens for protein–protein [1– 3] and protein complex interactions [4–7], high-throughput genetic interaction analyses [8–13], genome-wide measurements of gen ...
University of Debrecen - DEA
... In contrast to eukaryotic cells, prokaryotic cells have a simpler internal structure without organelles. However, prokaryotes differ from eukaryotes in many other ways as well. In most prokaryotic cells, DNA is present in circular molecule called the chromosome, usually only one. However it must be ...
... In contrast to eukaryotic cells, prokaryotic cells have a simpler internal structure without organelles. However, prokaryotes differ from eukaryotes in many other ways as well. In most prokaryotic cells, DNA is present in circular molecule called the chromosome, usually only one. However it must be ...
Directions and Questions for Lab 9 - San Diego Unified School District
... against the corner. Decant the stain directly to a sink drain and flush with water. e. Add distilled or tap water to the staining tray. To accelerate destaining, gently rock the tray. Destain until bands are distinct, with little background color. This will take between 20 and 30 minutes, depending ...
... against the corner. Decant the stain directly to a sink drain and flush with water. e. Add distilled or tap water to the staining tray. To accelerate destaining, gently rock the tray. Destain until bands are distinct, with little background color. This will take between 20 and 30 minutes, depending ...
Eukaryotic RNA Polymerases and their Promoters
... – Upstream promoter element (UPE) 100 bp farther upstream – Spacing between these elements is important ...
... – Upstream promoter element (UPE) 100 bp farther upstream – Spacing between these elements is important ...
DNA supercoil
DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.