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2nd problem set
2nd problem set

... a) ______________ : a sequence that immediately precedes a gene and indicates the start of transcription. b) ______________ : a protein that synthesizes a new strand of DNA. c) ______________: a molecule which can terminate a growing DNA strand. 7. Which one of the following molecules is NOT found i ...
DNA
DNA

... Many Origins Of Replication Origins of Replication ...
Genetic Mutations - Velma Jackson High
Genetic Mutations - Velma Jackson High

... cell that has had it’s nucleus removed  The egg is then stimulated by chemicals or electricity to cause it to divide  Next, it is implanted into the uterus of a female until further ...
Biological Molecules Test Review Test covers carbohydrates, lipids
Biological Molecules Test Review Test covers carbohydrates, lipids

... H. Draw a structural formula for each of the 3 types of carbohydrates. ...
Transcription and Translation
Transcription and Translation

... Transcription cont. 7. As synthesis proceeds, the RNA polymerase moves in the 3' to 5' direction on the copied strand. 8. When a specific terminator sequence is reached, transcription stops. 9. Enzymes and the single stranded RNA product are then released. 10. The orientation of the RNA is determin ...
Research Highlights: Highlights from the last year in nanomedicine
Research Highlights: Highlights from the last year in nanomedicine

Study Guide
Study Guide

... are locations where DNA replication is really taking place? Couldn't they represent areas where DNA polymerase molecules congregate when they're not doing anything?" In particular, it lays out the evidence in favor of point (i) -- that "the presence of foci was dependent on continued DNA synthesis." ...
DNA Know your Molecules
DNA Know your Molecules

... Type of RNA made ...
Structure and Function of DNA
Structure and Function of DNA

...  DNA stores all of the genetic information for the cell.  If it located in the nucleus but can also be found in the mitochondria and the chloroplast.  Serves as the blueprint for making proteins. ...
Cloning vectors share four common properties
Cloning vectors share four common properties

... (kilobases or kb [1000bp]) ...
IB Biology 11 SL (H) - Anoka
IB Biology 11 SL (H) - Anoka

... ● Explain the consequence of a base substitution mutation in relation to the processes of transcription and ● How genetic information is transmitted from parents to offspring through the processes of meiosis and translation, using the example of sickle-cell anemia fertilization as they relate to chr ...
RESTRICTION ENDONUCLEASES
RESTRICTION ENDONUCLEASES

... early 1970s and the recognition that these enzymes act as “molecular scissors”, always cutting DNA at specific locations (base sequences), was the key discovery which allowed the cloning of DNA to become a reality (Turner et al., 1997). Restriction enzymes are proteins that bind to a specific DNA se ...
Mutations
Mutations

... Phenotype gets worse and has younger onset with each generation ...
molecular_gene_cloning_restriction
molecular_gene_cloning_restriction

... early 1970s and the recognition that these enzymes act as “molecular scissors”, always cutting DNA at specific locations (base sequences), was the key discovery which allowed the cloning of DNA to become a reality (Turner et al., 1997). Restriction enzymes are proteins that bind to a specific DNA se ...
Document
Document

... 2. Biological - viruses or plasmids (ring of DNA in bacteria that carries non bacterial chromosomes) F. Gene Splicing - rejoining the cut DNA with viral DNA or plasmid (plasmid may have to use restriction enzymes also & can have "sticky" ends) G. DNA is then transferred to the host cell - when host ...
Chapter 28: Chromosomes
Chapter 28: Chromosomes

... – Boundary elements delimit areas of decompaction – Nucleosomes in the decompacted area unwind to allow initiation of transcription • Transcription factors (nonhistone proteins) unwind nucleosomes and dislodge histones at 5’ end of genes • Unwound portion is open to interaction with RNA polymerase w ...
DNA Technology
DNA Technology

... • Restriction enzymes cut DNA into fragments. The fragmented DNA is injected into wells in the gel. A current is sent through the gel and the fragments will move at different speeds that appear as bands under UV light. Bands can be matched up to identify criminals, bodies or fossils or to determine ...
Point Mutations
Point Mutations

Exam 3
Exam 3

... another. This shift alters the hydrogen bonding between bases which results in improper basepairing, allowing the tautomerized base to pair with bases other than the one it is normally paired with during DNA replication. Base analogues are compounds sufficiently similar to basepair with the correct ...
Gene Technology Study Guide KEY
Gene Technology Study Guide KEY

... is that this allows for DNA from other organisms to join this genome in order to make recombinant DNA.  How is recombinant DNA formed?  Recombinant DNA is formed when a restriction enzyme cuts the DNA from one organism and DNA from another organism is added to the sticky ends of the cut DNA. Once ...
How do organisms grow and heal themselves? What instructions do
How do organisms grow and heal themselves? What instructions do

... with roughly 4.5 billion base pairs. • Coil into structures we call chromosomes – Chromosomes are DNA wrapped around a protein called histones ...
dna adducts - dr
dna adducts - dr

... either exogenous (xenobiotic) or endogenous (including metabolites of xenobiotics). The effect of an adduct depends on it’s location on DNA: • An adduct on a gene will usually reduces or blocks that gene’s expression. • An adduct near (on the promotor region of) a gene may lead to over-expression of ...
Name: Period: ______
Name: Period: ______

... So far, we’ve learned that DNA is the genetic material that organisms inherit from their parents, but have you thought about what exactly is encoded for by this DNA? How do our cells use DNA as a set of instructions for life? How is the information in our DNA and genes used by our bodies? And what h ...
HL-SAN for DNA removal in protein purification - A4
HL-SAN for DNA removal in protein purification - A4

... The amount of HL-SAN needed for DNA removal from a cell extract or lysate depends on several factors; expression strain, target protein, lysis buffer composition, NaCl concentration, etc. The following is therefore considered as guidelines: Add 1000 U HL-SAN per ml sample with 0.3–0.75 M NaCl and in ...
Restriction Enzyme
Restriction Enzyme

... Plant Tumor Caused by Agrobacterium tumafaciens ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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