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Comparing Mitosis and Meiosis
Comparing Mitosis and Meiosis

... Four characteristics of genetic material: 1. Replication 2. Information storage 3. Information expression 4. Change (variation) by mutation ...
Bacterial recombination
Bacterial recombination

... Why do we care about homologous recombination?  Universal biological mechanism  Bacteria can pick up new genes  Biotechnology  Gene knockouts in mice via homologous ...
Ch. 13 – Biotechnology
Ch. 13 – Biotechnology

... §  restriction endonucleases §  discovered in 1960s §  evolved in bacteria to cut up foreign DNA §  “restrict” action of attacking organisms (viruses and other bacteria) §  How do bacteria protect their own DNA? §  Methylation ...
Q.No Genetic engineering is the technique of introducing foreign
Q.No Genetic engineering is the technique of introducing foreign

... the ability to dissolve most of the compounds in it. This is possible because of the polarity which water has. The molecule of the water comprise of two 10 elements hydrogen and oxygen. ...
The Avery and Hershey-Chase Experiments
The Avery and Hershey-Chase Experiments

... • Hershey and Chase provided further evidence that heredity material in bacteriophages was found in DNA, not in proteins. – Many did not accept Avery’s conclusions until 1952 when Alfred Hershey and Martha Chase conducted this experiment with bacteriophages (viruses that attack bacteria) ...
Plant Transformation
Plant Transformation

... and additional amino acids that direct the transport of the protein to the organelle inserted into the chromosomal DNA, and, after synthesis, the recombinant protein transported into the targeted ...
Genetic Engineering
Genetic Engineering

... The DNA is pulled through the agarose by running an electric current through the agarose gel. DNA has a negative charge DNA molecules migrate toward the anode which has a positive charge Large fragments of DNA move slowly through the agarose while small DNA fragments move quickly. A molecular weight ...
eprint_12_13279_954
eprint_12_13279_954

... pyrimidine bases on the opposite strands. A on one strand pairs by two hydrogen bonds with T on the opposite strand, or G pairs by three hydrogen bonds with C. The two strands of double-helical DNA are, therefore complementary. Because of complementarity, double-stranded DNA contains equimolar amoun ...
DNA Technology
DNA Technology

... organism, containing the recombinant DNA, into the organism into eukaryote.  Waiting until the eukaryotes genome has been changed by the invading ...
Name - Mr. Spechts world of Science
Name - Mr. Spechts world of Science

... chromosomes on to offspring (3) a loss of genetic information that will produce a genetic disorder in the offspring (4) an increase in the chromosome number of the organism in which this process occurs 15. A change in the order of DNA bases that code for a respiratory protein will most likely ...
Bio07_TR__U04_CH12.QXD
Bio07_TR__U04_CH12.QXD

... RNA is copied from DNA in a process called transcription. The enzyme RNA polymerase binds to DNA and separates the two strands. Then, RNA polymerase builds a strand of RNA using one strand of DNA as the template. The sequence of DNA that signals RNA polymerase where to bind and start making RNA is c ...
Common types of DNA damage Different types of repair fix different
Common types of DNA damage Different types of repair fix different

... Different types of repair fix different types of damage In increasing order of complexity of the problem: • direct repair of specific modification • base excision repair: missing or altered base • (oligo)nucleotide excision repair: distortion of B-DNA with damage on one strand • mismatch repair: bot ...
Molecular Biology -
Molecular Biology -

... (e.g. normal hemoglobin vs. sickle cell hemoglobin)  person's characteristics or traits (e.g. normal health vs. sickle cell anemia) 2. The double helix structure of DNA, transcription and translation all depend on base-pairing rules that match each type of nucleotide in DNA or RNA with another nucl ...
Genetics
Genetics

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Biotechnology Lab
Biotechnology Lab

... Topoisomerases can cut DNA once or twice • Either way can increase or decrease supercoiling • Dimers can be made or removed by topoisomerases ...
OC 28 Nucleic Acids
OC 28 Nucleic Acids

... a right-handed helix 2000 pm thick with 3400 pm per ten base pairs minor groove of 1200pm and major groove of 2200 pm ...
CH 12: Mendel and Heredity
CH 12: Mendel and Heredity

... 13. What is a point mutation and does it always change the protein that is made? ...
Ch 13 student notes
Ch 13 student notes

AP Biology Objectives
AP Biology Objectives

... 10. Describe the structure and function of tRNA, and ribosomes. 11. Describe initiation, elongation, and termination of translation, AND explain which enzymes, protein factors, and energy sources are needed for each stage. 12. Explain what determines the primary structure of a protein and describe h ...
Bacterial Genetics
Bacterial Genetics

Manipulating genes and cells (Kap. 10)
Manipulating genes and cells (Kap. 10)

... ¾ cloning of DNA ¾ PCR and PCR applications ¾ isolating cells and growing them in culture ¾ protein expression in recombinant cell lines ¾ genetically altered animals and plants ...
learning objectives
learning objectives

... A. The first step of genetic engineering is to cleave the DNA that the geneticist wishes to transfer. B. This process involves the use of restriction enzymes that bind specific sequences of nucleotides and split the DNA in that position. C. Since DNA is made up of complementary bases, both strands d ...
Unit #3 Retake Ticket Unit 3 Retake Ticket
Unit #3 Retake Ticket Unit 3 Retake Ticket

... ______, and ______ have specific roles in this process. Structure B/G, known as __________, is important because it carries the DNA message from the (A)_____________ to the _______________. There, the (G) _________ attaches to the surface of (C) ___________, which is made partly of the second type o ...
Biology 1 Exam III Summer2005(ch8-9-10-11).doc
Biology 1 Exam III Summer2005(ch8-9-10-11).doc

... 9) The two sister chromatids of a eukaryotic chromosome are connected at the: a) centromere. b) centriole. c) chiasma. d) telomere. e) centrosome. 10) DNA replication occurs in eukaryotic cells during: a) G1 phase. b) S phase. c) G2 phase. d) mitosis. e) G0 phase. 11) The intertwining (crossing over ...
Transposons: Mobile DNA DNA
Transposons: Mobile DNA DNA

... DNA transposons are able to transpose in direct, DNA-DNA manner and are present in prokaryotes and eukaryotes Two distinct mechanisms of transposition: •Replicative transposition – direct interaction between the donor transposon and the target site, resulting in copying of the donor ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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