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No Slide Title
No Slide Title

... Chargaff’s rules 1940s 1. Base composition of DNA varies from one species to another 2. DNA from different tissues of same species have same base composition 3. Base composition of DNA in given species does not change with age, nutritional state, environment 4. In all cellular DNAs, regardless of sp ...
CSI: SNAB - NKS | VLE - our Online Classroom
CSI: SNAB - NKS | VLE - our Online Classroom

... Using primers specific for intron 1 of the gene, the gene sequence for the intron can be amplified. The X chromosome gene, AMELX, gives rise to a 106 bp amplification product (amplicon) and the Y chromosome gene, AMELY, a 112 bp amplicon. Hence, the AMELX contains a 6 bp deletion in the intron 1. Wh ...
Cat Coat Color Genetics Part 1
Cat Coat Color Genetics Part 1

... cells into a small plastic ...
DNA: Information Molecule
DNA: Information Molecule

Slide 1
Slide 1

... Need to use different kits adapted to the size of the genome being analyzed. Like RAPD markers need to be converted to quick and easy PCR based marker ...
PicoPure DNA Extraction Kit
PicoPure DNA Extraction Kit

... DNA Extraction Kit. The DNA was used, without further purification, in PCR reactions to amplify a 125 bp human p53 gene fragment and a 245 bp human cystic fibrosis gene fragment. PCR products were separated and detected using the WAVE dHPLC System. (Data courtesy of Scott Hamlin and Nicolas Neckelma ...
Gene Technology Study Guide
Gene Technology Study Guide

... protein called green fluorescent protein (GFP) into various organisms. GFP, which is a substance naturally found in jellyfishes that live in the north Pacific Ocean, emits a green light when it its exposed to ultraviolet light. o Recombinant DNA - newly generated DNA molecule, with DNA from differen ...
Solutions  - MIT OpenCourseWare
Solutions - MIT OpenCourseWare

... The aspartic acid side chain is negatively charged at biological pH. This negatively charged group is highly hydrophilic and will most likely be oriented so that interaction with water is possible. Problem #4 Provide a sketch of a lipid bilayer. Label the polar and nonpolar regions. Solution ...
Slide ()
Slide ()

Green, Ed, NEADERTHAL DNA, UC Santa Cruz, June
Green, Ed, NEADERTHAL DNA, UC Santa Cruz, June

... one set of dna from mom and one set from dad... that is what DIPLOID means. where in that genome are these uniquely human characteristictics encoded... what makes humans unique genetically?. 35M SNP changes (single nucleotide polymorphisms.) 5M insertion/deletions 9 pericentric inversions and 1 chro ...
Transcription/Translation foldable
Transcription/Translation foldable

... foldable Fold your paper so the two ends meet in the middle. Label Transcription on one side and Translation on the other. ...
Applied Biosystems® Arcturus® PicoPure® DNA Extraction Kit
Applied Biosystems® Arcturus® PicoPure® DNA Extraction Kit

A Novel Interacting Protein With The DNA Mismatch Repair Gene
A Novel Interacting Protein With The DNA Mismatch Repair Gene

投影片 1 - NYMU BML
投影片 1 - NYMU BML

... Produced via “translation” of messenger RNA (mRNA) Each protein has one or more specific functions Form body’s major components Carbohydrate and lipid metabolism mediated by proteins ...
PowerPoint 演示文稿
PowerPoint 演示文稿

... Figure 1.36 The central dogma states that information in nucleic acid can be perpetuated or transferred, but the transfer of information into protein is irreversible. ...
Week 4 Pre-Lecture Slides
Week 4 Pre-Lecture Slides

DNA RNA Lecture Website
DNA RNA Lecture Website

... four 2. There are ___ different nucleotides (since there are four different nitrogenous bases). three nucleotides in 3. It was discovered that ______________ amino acid sequence must specify each __________. This would provide for ___ 64 possible combinations of amino acids. triplet of nucleotides i ...
5о end of mRNA 1 2 1 1 2 3 Protein Ribosome RNA
5о end of mRNA 1 2 1 1 2 3 Protein Ribosome RNA

... Sliding clamp holds DNA polymerase III in place ...
Electrophoretic stretching of DNA molecules using microscale T
Electrophoretic stretching of DNA molecules using microscale T

... 共measured17 to be 1.3± 0.2 s兲. In Fig. 3共c兲 we see that strong stretching occurs once De⬎ 0.5, similar to what is observed in hydrodynamic flows.8 We next tried to stretch molecules which have contour lengths much larger than 2 ⫻ w3 共40 ␮m兲. In Fig. 4 we show the stretching of a concatomer of ␭-DNA ...
DNA and RNA Chapter 12
DNA and RNA Chapter 12

ProteinSynthesis11
ProteinSynthesis11

... Check: Are there any T’s in your mRNA? There shouldn’t be!!! ...
2008 exam with answers
2008 exam with answers

... but not without the 5’ to 3’ exo. Therefore it must be the 5’ to 3’ exo of enzyme X that is critical in DNA replication, and cannot be replaced by action of another enzyme. The function of 5’ to 3’ exo is to degrade the primer. The other activities of enzyme X could be used for elongation and proof ...
Epigenetics of Coeliac Disease
Epigenetics of Coeliac Disease

... • It may translate the effects of risk factors in terms of molecular events. • It is feasable with the recent development of micro arrays/Si RNA knowledge. • There is no data published to date on this topic (pubmed June 2012). ...
discov5_lecppt_Ch16
discov5_lecppt_Ch16

... Genetic Engineering • A gene that is introduced into a GMO is called a transgene; therefore, GMO individuals are known as transgenic organisms • Scientists have been able to track gene expression using a light-producing protein known as green fluorescent protein (GFP), which is extracted from jell ...
Gene%20Sequencing[2]
Gene%20Sequencing[2]

... Made of deoxyribose, three phosphate groups, and a nitrogen base Double-stranded molecule; covalent bonds between ribose/phosphate backbone on outside; hydrogen bonds between nitrogen bases on inside ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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