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Epigenetics 101 - Nationwide Children`s Hospital
Epigenetics 101 - Nationwide Children`s Hospital

...  Methyl marks (CH3) added to cytosine  CpG islands located at the 5’ end of genes, typically contain promoter and first exons (gene coding regions)  1x108 marks per genome  50-60% of all human genes contain a CpG island  Tissue-specific patterns of CpG island methylation are ...
1. DNA SEQUENCER (Applied Biosystems, 3730xl DNA Analyzer)
1. DNA SEQUENCER (Applied Biosystems, 3730xl DNA Analyzer)

Section 13.2 Summary – pages 341
Section 13.2 Summary – pages 341

... in less than a day. ...
DNA Base Pairing Activity
DNA Base Pairing Activity

... 7. Depending on the size of the group, select 3‐5 bases on one side of the strand to “cut” out of  the strand and have that many other students ready to “insert” into the strand.  a. You make it so that each student in the strand that you “cut” out has something in  common (ie. red shirt, female, et ...
LLog3 - CH 3 - Immortal Genes
LLog3 - CH 3 - Immortal Genes

... arrangements of the four basic nucleotides: A, T, C, and G. To truly understand the four lettered language, one must understand genomes, genes, and how to read DNA. Some vocabulary that should be known to understand DNA is: proteins, bases, amino acids, triplet, and gene. One should also note the th ...
Blueprint for life - Siemens Science Day
Blueprint for life - Siemens Science Day

... Polynucleotide Backbone ...
ppt
ppt

... protein when engineered to contain inactivating mutations in both of its active sites. Dead Cas9 (dCas9) can be fused to: •activator or repressor domains for transcriptional down-regulation or activation . •fused to fluorescent domains, (eg. GFP), for livecell imaging of chromosomal loci. •fused chr ...
Introduction to Molecular Cell Biology (not tought by SK in 2010)
Introduction to Molecular Cell Biology (not tought by SK in 2010)

... Mitochondria have their own DNA, which replicates independent of the nuclear DNA 2. Genetic code of the mitochondria is different from the main code of the cell 3. Mitochondria have their own ribosomes on which some of the mitochondrial proteins are produced. Others are imported from the outside 4. ...
Chapter 25: Molecular Basis of Inheritance
Chapter 25: Molecular Basis of Inheritance

... Angiogenesis is the formation of new blood vessels to bring additional nutrients and oxygen to a tumor; cancer cells stimulate angiogenesis. Metastasis is invasion of other tissues by establishment of tumors at new sites. A patient’s prognosis is dependent on the degree to which the cancer has prog ...
2420 Topics for Examination II
2420 Topics for Examination II

... size? How is DNA transferred from the agarose gel to a special filter membrane? What is a Southern blot? Is bacterial probe DNA able to distinguish human target DNA from bacterial target DNA? Know that this specificity is due to base sequences which have unique homologies, preventing attachment of p ...
1. The Building Blocks of DNA
1. The Building Blocks of DNA

... relatively stable manner because there are enormous numbers of these bonds. It is important that the strands be associated through such weak interactions, since they have to be separated during DNA replication and during transcription into RNA The sugar-phosphate backbone, being connected by covalen ...
Notes - Dr. Bruce Owen
Notes - Dr. Bruce Owen

... − depending on which exons are spliced together after the introns are snipped out − yet most (over 98%) of our DNA does not code for proteins − about half of this is “junk” DNA − it is never transcribed to RNA − there are vast stretches that are simple repeating units − others that are seemingly ran ...
25 transcription, translation
25 transcription, translation

... Using DNA ...
APNucleic Acids
APNucleic Acids

here - IMSS Biology 2014
here - IMSS Biology 2014

Genetics = science of heredity - Suffolk County Community College
Genetics = science of heredity - Suffolk County Community College

... 1. Enzymes, gyrase and helicase, unwind the parental double helix at a site called the origin of replication. 2. Proteins stabilize the unwound parental DNA creating the replication fork. 3. Beginning with an RNA primer complementarily base paired to the single stranded parental DNA, the leading str ...
Manipulating DNA - Emerald Meadow Stables
Manipulating DNA - Emerald Meadow Stables

... Creating Recombinant DNA • In order to create Recombinant DNA, there needs to be: – DNA extraction • Cells opened to separate DNA from other cell parts – Cutting DNA • DNA too large to study, so biologists “cut” them into smaller fragments using restriction enzymes. Many restriction enzymes are kno ...
Gene Regulation of Eukaryotes
Gene Regulation of Eukaryotes

...  Proto-oncogene - is a normal gene that can become an oncogene due to mutations or increased expression. ...
1) - life.illinois.edu
1) - life.illinois.edu

... i). (5 Points). Which mechanism of transposition does this experiment support? Why? (Use a diagram or precise language to explain your answer). The result supports replicative transposition because a transposition event will transfer an element containing a single strand of Tn88-lacZ which is replic ...
Chapter 17 – Molecular genetics
Chapter 17 – Molecular genetics

... Some amino acids (AA) are coded for by more than one codon, while others, only by one Each set of 3 amino acids is called a reading frame ...
Genetic changes - Southington Public Schools
Genetic changes - Southington Public Schools

Chapter 17 – Molecular genetics
Chapter 17 – Molecular genetics

... called DNA ligase ...
Biology Study guide 2 with standards-DNA-evolution
Biology Study guide 2 with standards-DNA-evolution

... If a cat has a body cell of 80 chromosomes than they sperm will have? 40 If an egg of a fish has 100 chromosomes then the tail would have? 200 Mitosis: The Cell Cycle is a process where body cells grow, make copies of chromosomes, and divide to make new cells. Makes cells to heal injuries. In Interp ...
Exam 4
Exam 4

... A) carrying amino acids to ribosomes for protein synthesis. B) serving as a component of ribosomes. C) acting as the template to create DNA. D) splicing of pre-mRNA. E) all of these are functions of RNA. 14. Which of the following is NOT a difference between DNA and RNA? A) RNA nucleotides use a dif ...
Answer Key to Chapter 10 Reading
Answer Key to Chapter 10 Reading

... This means that an organism’s genes (genotype) largely determines the physical outcome of the character. 3. A biochemist identifies a new toxin that closes the nuclear pores of a cell’s nucleus. What cellular process would be disrupted by this toxin? Which one would not be? Briefly explain you ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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