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recombinant dna
recombinant dna

... This mixture is then cooled to about 65°C, enabling doublestranded DNA to reform. ...
Genetics notes, long version
Genetics notes, long version

... about base pairs. A base pair is two molecules that are connected to each other, forming a connection between the two sides of the DNA double helix. (as shown in the left and center drawings above). Because of the size and shape of the bases, they can only connect in one way – Adenine always pairs w ...
DNA! - Chapter 10
DNA! - Chapter 10

... Ribonucleic Acid, present in all living cells Single Stranded Helps with the creation of proteins! Has 3 of the same nitrogenous bases DNA has except uracil takes the place of thymine ...
Mid-Term Review L4
Mid-Term Review L4

... All your tests, quizzes, and papers are in your folders – feel free to take them (leave the manila folder though!) to study from Know your vocab! Vocab will help you understand what the questions are asking – if you don’t understand the question, it makes it much more difficult to answer it. The tes ...
How Does Evolution Occur? - Downtown Magnets High School
How Does Evolution Occur? - Downtown Magnets High School

... • DNA: code that forms your traits. • DNA makes up genes- set of instructions for one trait. • Chromosomes carry the genes. • Some traits are dominant (shows up in offspring) or recessive (doesn’t show). ...
Crossing natural barriers to genetic manipulations
Crossing natural barriers to genetic manipulations

... traits from one plant to another. gene can be spliced to the virus vector. Also, the added gene affects the cell-to-cell movement (invasiveness) of the virus, since the virus can no longer mature. Moreover, the virus is mainly limited to Cruciferae as host plants and therefore the prospects of infec ...
My Genetic Profile Worksheet
My Genetic Profile Worksheet

... strands. For example: DNA strands with the base sequence TTCAGGCAG will be attracted to any cDNA strands with the sequence AAGTCCGTC. In other words each DNA cluster will be attracted to cDNA that were made using mRNA sequences that were transcribed from the same gene. • The microarray is exposed to ...
Mitosis
Mitosis

... 6. ____________________________ - Discovered the shape of DNA through x-ray diffraction 7. ________________ and ___________ - discovered double helix- 3 dimensional shape & structure of DNA Figure 12-1 8. The structure labeled X in Figure 12-1 is a(an) ______________________ (monomer). 9. Monomers c ...
DNA - Mrs-Lamberts-Biology
DNA - Mrs-Lamberts-Biology

... DNA prior to cell division so the daughter cells both get a full set. The next two processes occur back to back, and this is how your genes make your body work. Each gene codes for specific protein(s) each individual cell needs to function properly and keep you alive. Many of these proteins are enz ...
4/17
4/17

... • Genetic distance is measured by recombination frequency • A relative map can be constructed based on genetic distances ...
dna & cell division
dna & cell division

... XI. DNA REPLICATION, cont.  DNA polymerases add complementary nucleotides to each side of the DNA molecule.  DNA polymerase can only add nucleotides to the 3’ end of the growing strand, so the daughter DNA is synthesized 5’ – 3’, which means parental DNA is “read” __ 3’ – 5’__.  This means only ...
Biology (Bio 315) S
Biology (Bio 315) S

... Stop codon). The reason for this is that (choose the best answer): A. mitchondrial protein mRNA is unusual in that the coding reading starts at the second AUG making it appear as thought the protein was smaller than it should have been. Thus, the protein size of the mitochondrial proteins was overes ...
Isolation and Purification of Nucleic Acids
Isolation and Purification of Nucleic Acids

... Cycle sequencing is chain termination sequencing performed in a thermal cycler.  Cycle sequencing requires a heat-stable DNA polymerase. ...
RNA polymerase
RNA polymerase

... Eukaryotic cells modify mRNA after transcription. Splicing of mRNA increases the number of different proteins an organism can produce. Gene expression is regulated by proteins that bind to specific base sequences in DNA. The environment of a cell and of an organism has an impact on gene expression. ...
recombinant dna
recombinant dna

... This mixture is then cooled to about 65°C, enabling doublestranded DNA to reform. ...
Ch 27 bacteria intro..
Ch 27 bacteria intro..

... 1.How does the bacterial chromosome compare to a eukarytotic chromosome? 2. How do variations arise in bacteria considering they reproduce mostly by asexual means? 3. What is a bacterial colony? (see reading on p 340 and Figure 18.12). 4. Briefly distinguish between the three mechanisms of transferr ...
DNA : The Genetic Material
DNA : The Genetic Material

... • 2. The DNA molecule separates into 2 complementary halves. (The areas where the double helix separates are called replication forks.) • 3. Free floating nucleotides join with the complementary nucleotides on the single strands. • 4. DNA polymerase (enzyme) binds to the separated chain and links th ...
jan4
jan4

... multiple ways that DNA can specify a single amino acid ...
nucleotides - UniMAP Portal
nucleotides - UniMAP Portal

...  Regions of DNA rich in GC repeats are often regulatory, binding specific proteins that initiate/block transcription. ...
Nature Rev.Genet. 8
Nature Rev.Genet. 8

... The AS-ICR is nonfunctional in males allowing the PWS-ICR to activate nearby genes The PWS-ICR promotes expression of an antisense Ube3a transcript in males ...
Exam #3 Review
Exam #3 Review

... strands of DNA can always serve as the template for the synthesis of the other strand. c. the hydrogen bonds holding the strands of nucleotides together can be broken in a process called denaturation or melting. d. all of the above. Practice: In what ways is RNA different than DNA? B. DNA can be tra ...
Chromosomal mutations
Chromosomal mutations

... Chromosome Structure DNA wrapped around histone proteins forming bead-like units or “nucleosomes” These are coiled forming a “super coil” which coils to form a “chromosome” ...
Exemplar exam questions – Chapter 7, Nucleic acids and proteins
Exemplar exam questions – Chapter 7, Nucleic acids and proteins

... carboxyl groups (–NH2 groups and –COOH groups). Hydrogen bonds are formed (these are weak). Form may become an α helix; or a β pleated sheet. Tertiary structure is the folding of the polypeptide; disulfide bridges give the structure stability. Quaternary structure is formed when several polypeptides ...
RNA 8.1 Identifying DNA as the Genetic Material
RNA 8.1 Identifying DNA as the Genetic Material

... – Messenger RNA (mRNA) carries the message that will be translated to form a protein. – Ribosomal RNA (rRNA) forms part of ribosomes where proteins are made. – Transfer RNA (tRNA) brings amino acids from the cytoplasm to a ribosome. ...
DNA Replication - Gadjah Mada University
DNA Replication - Gadjah Mada University

... usually contain 2 –6 genes, (up to 20 genes) these genes are transcribed as a polycistronic transcript.  relatively common in prokaryotes  rare in eukaryotes ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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