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NCEA Level 1 Science (90948) 2016
NCEA Level 1 Science (90948) 2016

... Sexual reproduction involves combining DNA from two parents using gametes. These gametes (sex cells) are formed during meiosis. Gametes have only one set of chromosomes, and so these can be combined with another parent to make a unique individual. This increases variation [OR crossing over OR indepe ...
Notes
Notes

... ◦ Gel Electrophoresis: a technique in which DNA is cut using a restriction enzyme and separated into bands to create a banding pattern. ◦ Southern Blotting: a technique in which DNA fragments in an electrophoresis gel are transferred to a membrane to check for a specific gene sequence using a marker ...
Exam1 2012 Life Student
Exam1 2012 Life Student

... ____ 88. After the DNA is replicated, and it condenses in prophase, two identical rods of DNA are seen. These are a. sister chromatids. b. centromeres. c. spindle fibers. d. kinetochores. e. chromatin. ____ 89. Spindle fibers a. are composed of DNA. b. only appear during interphase. c. attach to th ...
Practical II - Faculty Websites
Practical II - Faculty Websites

... 10. (351) The following figure shows an agarose gel. Three fragments of DNA (A, B, and C) have been loaded into wells toward the top of the gel, and the positive pole of the electric field is at the bottom. The sizes of the three DNA fragments are indicated below. (a) After a defined period of migra ...
File
File

... (b) What were the phenotype and genotype of the F1 mice? If the RFLP marker and disease gene are linked, show the arrangement of disease/normal alleles and RFLP markers on the homologous chromosomes. Answer: (a) Yes, it appears to be linked to the disease gene. There are several explanations, such a ...
Biology 120 Lab Exam 2 Review Session
Biology 120 Lab Exam 2 Review Session

IN HUMAN EVOLUTION
IN HUMAN EVOLUTION

... times aroused suspicion. Some researchers of contamination—Stringer, for one, had now sequence entire ancient populations, at first refused access to fossils, and the left a trail of his own DNA on fossils across as Reich’s lab is doing, ancient DNA is addCzech police searched his belongings, saying ...
Modeling Mutations Activity
Modeling Mutations Activity

PreAssessment - Boone County Schools
PreAssessment - Boone County Schools

... DNA of the offspring is identical to that of the parent Some plants reproduce this way Requires two different parent (sex) cells. DNA of the offspring is different than that of both parents. Each time offspring are formed, a new combination of traits is passed. Advantage- variation among offspring a ...
central themes in physiology
central themes in physiology

Genetic mechanisms
Genetic mechanisms

... RNA polymerase binds to promoter region upstream of the gene 2. RNA polymerase adds nucleotides complementary to the template strand of a segment of DNA in the 5’ to 3’ direction (downstream of promoter) 3. Uracil is placed as adenine’s complement (U with A) 4. At termination, RNA polymerase recogni ...
Topic 10: « MODERN METHODS OF DNA DIAGNOSIS OF
Topic 10: « MODERN METHODS OF DNA DIAGNOSIS OF

genotypes
genotypes

... consider two steps. The first is to determine if the disorder is autosomal or X-linked. • If the disorder is X-linked most of the males will have the disorder because the Y-chromosome cannot mask the affects of an affected X-chromosome. A female can have the disorder, but it would be a very low perc ...
Unit 5 Notes Outline File
Unit 5 Notes Outline File

... placental cells 3. Fetal Cell Sorting - fetal cells are separated from _____________________________ - can be found in _____ of all pregnancies - still experimental Polyploidy – abnormal multiples of the _______________ number - ___________ in humans (17% of all miscarriages) A) ____________________ ...
Reaction discovery enabled by DNA
Reaction discovery enabled by DNA

... Sequences encoding bond-forming substrate pairs were amplified by PCR with a DNA primer labeled with the cyanine fluorophore Cy3 ...
Exam I - chem.uwec.edu
Exam I - chem.uwec.edu

... of proteins make them particularly well-suited to carrying out their biological roles. The four nucleotides that are used to make DNA have very similar chemical and physical properties. Consequently, DNA is chemically inert and its structure looks pretty much the same, regardless of the nucleotide s ...
Lecture 1. - Government Degree College Pulwama
Lecture 1. - Government Degree College Pulwama

... sequences that would not otherwise be found in the genome. Recombinant DNA is the general name for a piece of DNA that has been created by the combination of at least two strands. Recombinant DNA molecules are sometimes called as Chimeric DNA, because they can be made of material from two different ...
Activity #5b. Plasmid DNA Isolation, Restriction Enzyme Digestion
Activity #5b. Plasmid DNA Isolation, Restriction Enzyme Digestion

... on the pGLO plasmid encode a beta-lactamase enzyme and the green fluorescent protein. Beta-lactamase is an enzyme that breaks down members of beta-lactam family of antibiotics, which includes ampicillin, penicillin and amoxicillin. These antibiotics are used to treat infections because they normally ...
No Slide Title
No Slide Title

... The distance separating the -35 and -10 sites is between 16-18 bp in 90% of promoters. In the exceptions it can go down to 15 or up to 20. Sequence itself is not important. Some promoters have an A-T-rich sequence located farther upstream. It is called UP element and interacts with a subunit of RNA ...
Mapping Mutations in the HIV RNA
Mapping Mutations in the HIV RNA

... When we can determine that a given node in the solution space does not lead to the optimal solutioneither because the given solution and all its successors are infeasible or because we have already found a solution that is guaranteed to be better than any successor of the given solution. In such cas ...
Chapter 11: DNA and the Language of Life - Rebecca Waggett
Chapter 11: DNA and the Language of Life - Rebecca Waggett

... Let’s celebrate with a Super Bowl Activity that you can use in your classroom to: *hook your students to study Molecular Genetics *encourage students to model protein folding and inheritance patterns ...
Comparison of three methods for DNA extraction
Comparison of three methods for DNA extraction

... Their inspection revealed that methods B and C (1.63 and 1.67) produce relatively less contaminated DNA than method A (1.33). Although variations in sample quality and quantity can definitely influence the pu-rity of the obtained DNA, this factor can be ruled out in this occasion, given that identic ...
Comparative Genomic Hybridization for
Comparative Genomic Hybridization for

SBARS: fast creation of dotplots for DNA sequences on different
SBARS: fast creation of dotplots for DNA sequences on different

... where fi , gi are two fragments of GC-content. Note that 0    1, as fi  W1 and the number of terms in the sum is equal to W2. Therefore, the distance does not depend on the sizes of the windows. For recognition of repeats, the following decision rule is used: if 5" where " is a threshold, then ...
0101BWhat characterizes a prokaryotic cell
0101BWhat characterizes a prokaryotic cell

... d) the number of isotopes of the atom b) the number of electrons in the outermost shell e) none of the above is correct c) the number of neutrons in the nucleus __21) The atomic number of nitrogen is 7. 15N is heavier than 14N because 15N has: a) eight protons b) eight neutrons c) seven protons d) s ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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