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A Physical Gene Map of the Bacteriophage P22 Late
A Physical Gene Map of the Bacteriophage P22 Late

... of P22 DNA by EcoRI has been shown to be a consequence of P22 DNA packaging (Jackson et al. 1978a). Because mature P22 DNA molecules are linear and circularly permuted, the cleavage map is circular (Jackson et al., 1978b). These mature chromosomes are generated by sequential headful packaging from a ...
Characterisation of Aeromonas species isolated from diseased fish
Characterisation of Aeromonas species isolated from diseased fish

... further research to test for Minimal Inhibitory Concentrations (MIC) are needed in future to assess the clinical relevance of above mentioned strains. On the basis of biochemical and morphological tests, several of these isolates have been demonstrated to be identical, but genetic studies like PCR-b ...
APGO Case book
APGO Case book

... PE findings of urethrocele and mild cystocele are very common in older women who have had children. This is best detected on physical exam using a Sims speculum and having the patient Valsalva. This allows one to examine the movement and degree of prolapse of the anterior and posterior vaginal wall, ...
Mechanisms of Transcription-Replication
Mechanisms of Transcription-Replication

... throughout the life cycle. The speed of the replication fork progression in Escherichia coli is ⬃1,000 bp per s (16), while the elongation rate for RNA polymerase is just 50 nucleotides (nt) per s (15); i.e., replication is approximately 20-fold faster than transcription. Since the two processes pro ...
Product description P003-D1 MLH1-MSH2-v01 - MRC
Product description P003-D1 MLH1-MSH2-v01 - MRC

... containing MLH1 or MSH2 exon deletions or amplifications: http://www.nibsc.org/documents/ifu/11-218xxx.pdf. As an alternative, an artificial positive duplication DNA sample for MLH1 and MSH2 (product name SD027) can be ordered from MRC-Holland. This FOR RESEARCH USE ONLY SD027 DNA will show a duplic ...
Xq28 duplications
Xq28 duplications

... with an Xq28 duplication page 19). One paper reported that six out of 11 boys with an Xq28 duplication died (from secondary recurrent respiratory infections or pneumonia) before 25 years of age (Friez 2006). In this report the boys also had severe functional problems of the bowel and/or bladder whic ...
Biology Final Exam Review Questions Answer Section SHORT
Biology Final Exam Review Questions Answer Section SHORT

... The nucleotides in a strand of DNA are joined by covalent bonds between their sugar and phosphate groups, and by hydrogen bonds between the complimentary bases. PTS: 1 DIF: L3 REF: p. 344 OBJ: 12.2.1 Identify the chemical components of DNA. STA: CA.BIO.5.a TOP: Foundation Edition BLM: synthesis 31. ...
Exclusion of known corneal dystrophy genes in an autosomal
Exclusion of known corneal dystrophy genes in an autosomal

... Purpose: With advances in phenotyping tools and availability of molecular characterization, an increasing number of phenotypically and genotypically diverse inherited corneal dystrophies are described. We aimed to determine the underlying causative genetic mechanism in a three-generation pedigree af ...
(Pan) Salmonella and Salmonella Serotype Typhi–Specific Real
(Pan) Salmonella and Salmonella Serotype Typhi–Specific Real

... Salmonella species are important causes of enteritis throughout the world. In addition, Salmonella enterica serotypes Typhi and Paratyphi are important causes of enteric fever in underdeveloped countries that lack adequate sewage disposal and water treatment facilities.1,2 It is standard practice in ...
Genetic studies of psoriasis and psoriatic arthritis
Genetic studies of psoriasis and psoriatic arthritis

... The studies in this thesis aimed at refining two susceptibility loci for psoriasis identified with linkage analysis, 3q21 and 5q31-32, with a special emphasis on the PSORS5 region on chromosome 3q21. Another purpose was to investigate whether several autoimmuneassociating genes and genomic regions a ...
Novel pathogen-specific primers for the detection of Agrobacterium
Novel pathogen-specific primers for the detection of Agrobacterium

... crown gall (BURR et al. 1998). Besides A. vitis, Agrobacterium tumefaciens may also occur on grapevines as causative agent of crown gall disease (SZEGEDI et al. 2005). The polymerase chain reaction (PCR) has widely been used to test for the presence of various plant pathogens to select clean plant m ...
Application for laboratory to join the
Application for laboratory to join the

... What is the ISO certification number? What is the date on the most recent schedule of accreditation? Were all the tests that are being proposed for service (appendix 1) declared at the time of the last inspection using the current methodology? ...
Ectopic Pregnancy - Society for Reproductive Endocrinology and
Ectopic Pregnancy - Society for Reproductive Endocrinology and

... for methotrexate treatment, a woman needs to be in stable condition with no evidence of internal bleeding or severe pain. She also needs to maintain communication with her physician during the treatment protocol and be able to return for follow-up blood tests after treatment. Methotrexate is a drug ...
Fulltext - Jultika
Fulltext - Jultika

... spectrum of the phenotypes caused by mutations in the COL2A1 gene coding for collagen II, the main cartilage collagen, is relatively well defined, but there is little data on the phenotypes caused by collagen IX and XI mutations. The structure of the human COL11A1 gene coding for the α1 chain of col ...
In vitro fertilization (IVF) in mammals: epigenetic and developmental
In vitro fertilization (IVF) in mammals: epigenetic and developmental

Warren, ST and Ashley, CT: Triplet repeat expansion mutations: The example of fragile X syndrome. Annual Review of Neuroscience 18:77-99 (1995).
Warren, ST and Ashley, CT: Triplet repeat expansion mutations: The example of fragile X syndrome. Annual Review of Neuroscience 18:77-99 (1995).

... eGG repeat of FMRl across normal populations revealed that it was highly polymorphic, with repeat sizes ranging from 6-54 triplets and a mode of 30 (Fu et al 1991, Brown et al 1993, Jacobs et al 1993, Snow et al 1993). In analysis of repeat sizes in fragile X pedigrees, Fu et al (1991) discovered th ...
T-box-mediated control of the anabolic proline biosynthetic genes of
T-box-mediated control of the anabolic proline biosynthetic genes of

... the isolation of chromosomal DNA from B. subtilis were carried out according to standard procedures (Sambrook et al., 1989). The nucleotide sequences of cloned PCR fragments and of mutations generated via site-directed mutagenesis were verified by the chaintermination method using the Thermo Sequena ...
Chance and Necessity in Arthur Peacocke`s Scientific Work
Chance and Necessity in Arthur Peacocke`s Scientific Work

... known to induce mutations in the DNA, so this clearly was a continuation of his previous interest in DNA damage. More importantly, these dyes and how they bind to DNA gave important clues about the actual structure-function relationship of the DNA molecule. As an aside, it is interesting to note tha ...
Comparison of different PCR tests for detecting Shiga toxin
Comparison of different PCR tests for detecting Shiga toxin

... the need to find a single specific DNA sequence to identify EHEC O157. Moreover, one of the major drawbacks of the published PCR-based approaches for detection of STEC O157 is lack of internal amplification control (IAC) which is required in order to monitor false negatives that may be caused by PCR ...
Nerve activates contraction
Nerve activates contraction

... switches places with a small fragment from the tip of chromosome 9. ...
htp Pseudomonas aeruginosa AMANDA SWANEPOEL
htp Pseudomonas aeruginosa AMANDA SWANEPOEL

... opportunistic pathogen of humans, causing infections in immunocompromised patients such as those with cancer or AIDS and those suffering from cystic fibrosis and burns (Van Delden and Iglewski, 1998; Ramsey and Wozniak, 2005), but it has also been shown to infect plants and insects (Schroth et al., ...
Chapter 4 - DORAS
Chapter 4 - DORAS

... haemin which was prepared at a concentration of 20 mM in 0.1 M sodium hydroxide was added to the wells. The bioassays were incubated for 12-24 hours at 37oC. Antibiotics were used at the following concentrations for M63 minimal media bioassays: spectinomycin 50 µl/ml, amplicillin 50 µl/ml, kanamycin ...
Recurrent Pregnancy Loss and Its Relation to Combined Parental
Recurrent Pregnancy Loss and Its Relation to Combined Parental

... Magna Pure Compact (Roche) (Invitek).  Twelve thrombophilic genes were simultaneously amplified in a biotinlabelled single multiplex amplification reaction which is based on the reversehybridization principle and by Real Time PCR, LightCycler 2.0 methods (Roche). ...
Introduction - Stellenbosch University
Introduction - Stellenbosch University

... https://www.ncbi.nlm.nih.gov/books/NBK179288/ • use esearch to find hemoglobin proteins • use pipe (|) to efetch to retrieve the proteins in the FASTA format • use head to display six lines of the output ...
392 final review game 2012
392 final review game 2012

... type O what are the possible blood types for their child? ...
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Cell-free fetal DNA

Cell-free fetal DNA (cffDNA) is fetal DNA circulating freely in the maternal blood stream. It can be sampled by venipuncture on the mother. Analysis of cffDNA provides a method of non-invasive prenatal diagnosis.cffDNA originates from the trophoblasts making up the placenta. It is estimated that 2-6% of the DNA in the maternal blood is fetal in origin. The fetal DNA is fragmented and makes its way into the maternal bloodstream via shedding of the placental microparticles into the maternal bloodstream (figure 1). Studies have shown that cffDNA can first be observed as early as 7 weeks gestation, and the amount of cffDNA increases as the pregnancy progresses. cffDNA diminishes quickly after the birth of the baby, so that it is no longer detectable in the maternal blood approximately 2 hours after birth. cffDNA is significantly smaller than the maternal DNA in the bloodstream, with fragments approximately 200bp in size. Many protocols to extract the fetal DNA from the maternal plasma use its size to distinguish it from the maternal DNA.Studies have looked at, and some even optimized, protocols for testing non-compatible RhD factors, sex determination for X-linked genetic disorders and testing for single gene disorders. Current studies are now looking at determining aneuploidies in the developing fetus. These protocols can be done earlier than the current prenatal testing methods, and have no risk of spontaneous abortion, unlike current prenatal testing methods. Non-invasive prenatal diagnosis (NIPD) has been implemented in the UK and parts of the US; it has clear benefits above the standard tests of chorionic villi sample (CVS) and amniocentesis which have procedure-related miscarriage risks of about 1 in 100 pregnancies and 1 in 200 pregnancies, respectively.As a method of prenatal diagnosis, cell-free fetal DNA techniques share the same ethical and practical issues, such as the possibility of prenatal sex discernment and sex selection.
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